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The map label for this gene is uvrD [H]

Identifier: 54026506

GI number: 54026506

Start: 4705542

End: 4707692

Strand: Reverse

Name: uvrD [H]

Synonym: nfa45330

Alternate gene names: 54026506

Gene position: 4707692-4705542 (Counterclockwise)

Preceding gene: 54026508

Following gene: 54026505

Centisome position: 78.18

GC content: 73.27

Gene sequence:

>2151_bases
ATGATGGTGACCGTGCCCGCCCTCGATCTCGCCGCCCTGGACCCCGAGCAGGCCGCCGCGGTCCGCGCACCGCGCGGGCC
GGTGTGTGTGCTGGCCGGCGCGGGCACCGGCAAGACCAGGACCATCACCTACCGCATCGCGCACCTGGTCGAGGCAGGCC
ACGTGAAGGCCGATCAGGTGCTGGCGGTCACCTTCACCGCACGGGCGGCGGGGGAGTTGCGAGGCAGGCTGCGCGCGCTC
GGCCTGGGCGGCGCCGCCAACCAGGTGCAGGCGCGCACCTTCCACGCCGCCGCGTTGCGTCAGCTCAAGTACTTCTGGCC
GCAGGTCGTCGGCGAGGTGCCGTGGCGGCTGCTCGAGGCCAAGTTCCCGGTGGTCGCCCAGGCCGCGCACCGCGTCGGCC
TGTCCACGGCCACCGAGAGCGTGCGCGATCTGCTCACCGAGATCGAGTGGGCGAAGTCCTCCCTCGTCGCCCCCGAGGAC
TATCCGGCCGCGGCCGCCGCCCACCACCGCGACATCCCCTACGACGCCGGGCGCATCGCCGCGGTCTACACCGCCTACGA
ATCGCTGAAGACCACACCCGAGGGCCTGCTGCTCGACTTCGACGACCTGCTCCTGCACACCGCCGCGGCGCTGGAGGACT
TCCCCGCCGTCGCCGACGAATTCCGCGGCCGGTACCGCAGTTTCGTCGTCGACGAATACCAGGACGTCACCCCGCTGCAA
CAGCGGGTACTCGACGCCTGGCTGGGCGAGCGCGACGACCTCACCGTCGTCGGCGACGCGAACCAGACGATCTACTCCTT
CACCGGCGCCACCCCGTCCTACCTGCTCGACTTCTCCCGCCGCTTCCCGGACGCCACGGTGGTGCGGCTCGAGCGCGACT
ATCGCTCCACCCCGCAGGTGGTCTCGCTGGCCAACCGCGTCATCGGCGCGGCCCGCGGCCGCATCGCCGGTACCCGGCTG
CAACTGGTCGGCCAGCGCCCCGACGGGCCGGAACCGGAGTTCGCCGAATACGACGACGCCCCCGCCGAGGCCGCCGCGGT
CGCCGCGAAGATCGAGCGGCTCGTCGCGGCGGGCACCCCGCCCGCGGAGATCGCGGTGCTGTACCGGATCAACGCGCAGT
CGGAGGCCTACGAACAGGCATTGGCCGAACGCGGCATCCCGTTCCAGGTGCGCGGCGGCGAAGGCTTCTACCAGCGTGCC
GAGGTGCGGCAGGCGCTCGGGGCGCTGCGGCAGGCCGCGTCTCGCGACGATCTGCCCGCCGAGGCGCGCACGGGTGCGGC
CCTGGTGCGGTTGGTGCGCGCGGCCCTGGCCCCGGTCGGGCTCACCGCCACCGAACCGGCGGGCGCGCAGGCCAGGGAGC
GCTGGGCATCGCTGACGGCCCTGGTGACCCTCACCGAGGAACTGGTCGCCCACGACGCGGCGCTCGACCTGACCGGCCTG
CTCCGTGAGCTGGCCGCCCGCGCCGAGGCCAGGCATCCACCGACAGTGCAGGGCGTCACGCTGGCCTCGCTGCACGCCGC
CAAGGGCCTGGAATGGGATGCGGTGTTCCTGGTCGGTCTCACCGACGGCACGCTGCCGATCGCGCACGTGCTCGCCGACG
ACGGCTCGGTCGCCGATGTCGCCGCCCTCGAGGAGGAGCGCAGGCTGCTCTACGTCGGCGTCACGCGGGCGCGGGAGTAC
CTGCGGCTGTCCTGGTCGCTGTCCCGCACCGAGGGCGGCCGACGCTCGCGCAGGCGGTCGCGCTTCCTCAACGGCCTGGT
CCCGGACGACTCGCCCGCCTCGCGCGTGGCCGCGCCCGCCATGCGGCCCTCCGAGGTGAACCGCCCGCGCTGCCGCGTGT
GCGCGAAGCCGCTGATCGGCACCTACGCGACCATGCTCGGCCGGTGCCGCCGCTGCCCGGCCGAGGTGGACGCGGACCTG
CTGGCCGCGCTGCGGGAATGGCGCGCCGAGAAGGCCGGCGAACTGGGGGTGCGGGAGTTCGTCGTGTTCACCGACACCAC
CCTCACCGCGATCGCCGAGCAACTGCCCGCCGACGACCGGGCACTGGCCGGAATCGCGGGCATCGGCGCGAAGAAGATCG
TCGACTACGGAGCCGAGGTACTGGCGATCGTGGCGTCGCGAGTACGGTCGGGATCACAAAACCGCAGGTAG

Upstream 100 bases:

>100_bases
TCGTCACGGCGGGGTTCACATCGGTCACAGCCGATACAACGCGCTGTGGTCCGGTCCTGTTCCACATCCCGCCGCCCCGG
TGGTGTCGGGGCGGACTGCC

Downstream 100 bases:

>100_bases
AAAATAGGTTGTCGGTTTCGCGGACAACGCATATGGTGGTTTCACGCACCGGGAGGGTATTCCGGCGCGCCAGTTTCGAC
ACCTGATCCACCGATCGGCG

Product: putative ATP-dependent DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 716; Mature: 716

Protein sequence:

>716_residues
MMVTVPALDLAALDPEQAAAVRAPRGPVCVLAGAGTGKTRTITYRIAHLVEAGHVKADQVLAVTFTARAAGELRGRLRAL
GLGGAANQVQARTFHAAALRQLKYFWPQVVGEVPWRLLEAKFPVVAQAAHRVGLSTATESVRDLLTEIEWAKSSLVAPED
YPAAAAAHHRDIPYDAGRIAAVYTAYESLKTTPEGLLLDFDDLLLHTAAALEDFPAVADEFRGRYRSFVVDEYQDVTPLQ
QRVLDAWLGERDDLTVVGDANQTIYSFTGATPSYLLDFSRRFPDATVVRLERDYRSTPQVVSLANRVIGAARGRIAGTRL
QLVGQRPDGPEPEFAEYDDAPAEAAAVAAKIERLVAAGTPPAEIAVLYRINAQSEAYEQALAERGIPFQVRGGEGFYQRA
EVRQALGALRQAASRDDLPAEARTGAALVRLVRAALAPVGLTATEPAGAQARERWASLTALVTLTEELVAHDAALDLTGL
LRELAARAEARHPPTVQGVTLASLHAAKGLEWDAVFLVGLTDGTLPIAHVLADDGSVADVAALEEERRLLYVGVTRAREY
LRLSWSLSRTEGGRRSRRRSRFLNGLVPDDSPASRVAAPAMRPSEVNRPRCRVCAKPLIGTYATMLGRCRRCPAEVDADL
LAALREWRAEKAGELGVREFVVFTDTTLTAIAEQLPADDRALAGIAGIGAKKIVDYGAEVLAIVASRVRSGSQNRR

Sequences:

>Translated_716_residues
MMVTVPALDLAALDPEQAAAVRAPRGPVCVLAGAGTGKTRTITYRIAHLVEAGHVKADQVLAVTFTARAAGELRGRLRAL
GLGGAANQVQARTFHAAALRQLKYFWPQVVGEVPWRLLEAKFPVVAQAAHRVGLSTATESVRDLLTEIEWAKSSLVAPED
YPAAAAAHHRDIPYDAGRIAAVYTAYESLKTTPEGLLLDFDDLLLHTAAALEDFPAVADEFRGRYRSFVVDEYQDVTPLQ
QRVLDAWLGERDDLTVVGDANQTIYSFTGATPSYLLDFSRRFPDATVVRLERDYRSTPQVVSLANRVIGAARGRIAGTRL
QLVGQRPDGPEPEFAEYDDAPAEAAAVAAKIERLVAAGTPPAEIAVLYRINAQSEAYEQALAERGIPFQVRGGEGFYQRA
EVRQALGALRQAASRDDLPAEARTGAALVRLVRAALAPVGLTATEPAGAQARERWASLTALVTLTEELVAHDAALDLTGL
LRELAARAEARHPPTVQGVTLASLHAAKGLEWDAVFLVGLTDGTLPIAHVLADDGSVADVAALEEERRLLYVGVTRAREY
LRLSWSLSRTEGGRRSRRRSRFLNGLVPDDSPASRVAAPAMRPSEVNRPRCRVCAKPLIGTYATMLGRCRRCPAEVDADL
LAALREWRAEKAGELGVREFVVFTDTTLTAIAEQLPADDRALAGIAGIGAKKIVDYGAEVLAIVASRVRSGSQNRR
>Mature_716_residues
MMVTVPALDLAALDPEQAAAVRAPRGPVCVLAGAGTGKTRTITYRIAHLVEAGHVKADQVLAVTFTARAAGELRGRLRAL
GLGGAANQVQARTFHAAALRQLKYFWPQVVGEVPWRLLEAKFPVVAQAAHRVGLSTATESVRDLLTEIEWAKSSLVAPED
YPAAAAAHHRDIPYDAGRIAAVYTAYESLKTTPEGLLLDFDDLLLHTAAALEDFPAVADEFRGRYRSFVVDEYQDVTPLQ
QRVLDAWLGERDDLTVVGDANQTIYSFTGATPSYLLDFSRRFPDATVVRLERDYRSTPQVVSLANRVIGAARGRIAGTRL
QLVGQRPDGPEPEFAEYDDAPAEAAAVAAKIERLVAAGTPPAEIAVLYRINAQSEAYEQALAERGIPFQVRGGEGFYQRA
EVRQALGALRQAASRDDLPAEARTGAALVRLVRAALAPVGLTATEPAGAQARERWASLTALVTLTEELVAHDAALDLTGL
LRELAARAEARHPPTVQGVTLASLHAAKGLEWDAVFLVGLTDGTLPIAHVLADDGSVADVAALEEERRLLYVGVTRAREY
LRLSWSLSRTEGGRRSRRRSRFLNGLVPDDSPASRVAAPAMRPSEVNRPRCRVCAKPLIGTYATMLGRCRRCPAEVDADL
LAALREWRAEKAGELGVREFVVFTDTTLTAIAEQLPADDRALAGIAGIGAKKIVDYGAEVLAIVASRVRSGSQNRR

Specific function: Has both ATPase and helicase activities. Unwinds DNA duplexes with 3' to 5' polarity with respect to the bound strand and initiates unwinding most effectively when a single-stranded region is present. Involved in the post-incision events of nucleotide exc

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI2367296, Length=666, Percent_Identity=28.5285285285285, Blast_Score=186, Evalue=6e-48,
Organism=Escherichia coli, GI48994965, Length=457, Percent_Identity=31.7286652078775, Blast_Score=173, Evalue=3e-44,
Organism=Saccharomyces cerevisiae, GI6324477, Length=404, Percent_Identity=24.7524752475248, Blast_Score=129, Evalue=2e-30,
Organism=Saccharomyces cerevisiae, GI6322369, Length=455, Percent_Identity=26.1538461538462, Blast_Score=124, Evalue=7e-29,

Paralogues:

None

Copy number: 3000 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR002121
- InterPro:   IPR014016
- InterPro:   IPR010997 [H]

Pfam domain/function: PF00570 HRDC; PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 77228; Mature: 77228

Theoretical pI: Translated: 6.92; Mature: 6.92

Prosite motif: PS50967 HRDC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMVTVPALDLAALDPEQAAAVRAPRGPVCVLAGAGTGKTRTITYRIAHLVEAGHVKADQV
CEEEECCCHHHHCCCHHHHHHCCCCCCEEEEECCCCCCCEEEEHHHHHHHHHCCCCCCCE
LAVTFTARAAGELRGRLRALGLGGAANQVQARTFHAAALRQLKYFWPQVVGEVPWRLLEA
EEEEEEHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
KFPVVAQAAHRVGLSTATESVRDLLTEIEWAKSSLVAPEDYPAAAAAHHRDIPYDAGRIA
CCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCCCCCHHH
AVYTAYESLKTTPEGLLLDFDDLLLHTAAALEDFPAVADEFRGRYRSFVVDEYQDVTPLQ
HHHHHHHHHCCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
QRVLDAWLGERDDLTVVGDANQTIYSFTGATPSYLLDFSRRFPDATVVRLERDYRSTPQV
HHHHHHHCCCCCCEEEEECCCCEEEEECCCCHHHHHHHHHHCCCCEEEEEEHHCCCCHHH
VSLANRVIGAARGRIAGTRLQLVGQRPDGPEPEFAEYDDAPAEAAAVAAKIERLVAAGTP
HHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCC
PAEIAVLYRINAQSEAYEQALAERGIPFQVRGGEGFYQRAEVRQALGALRQAASRDDLPA
CHHEEEEEEECCCHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCC
EARTGAALVRLVRAALAPVGLTATEPAGAQARERWASLTALVTLTEELVAHDAALDLTGL
HHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LRELAARAEARHPPTVQGVTLASLHAAKGLEWDAVFLVGLTDGTLPIAHVLADDGSVADV
HHHHHHHHHCCCCCCCCCCCHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHCCCCCHHHH
AALEEERRLLYVGVTRAREYLRLSWSLSRTEGGRRSRRRSRFLNGLVPDDSPASRVAAPA
HHHHHCCEEEEEEHHHHHHHHHEEEEEHHCCCHHHHHHHHHHHCCCCCCCCCHHHHHCCC
MRPSEVNRPRCRVCAKPLIGTYATMLGRCRRCPAEVDADLLAALREWRAEKAGELGVREF
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCEEE
VVFTDTTLTAIAEQLPADDRALAGIAGIGAKKIVDYGAEVLAIVASRVRSGSQNRR
EEEECHHHHHHHHHCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MMVTVPALDLAALDPEQAAAVRAPRGPVCVLAGAGTGKTRTITYRIAHLVEAGHVKADQV
CEEEECCCHHHHCCCHHHHHHCCCCCCEEEEECCCCCCCEEEEHHHHHHHHHCCCCCCCE
LAVTFTARAAGELRGRLRALGLGGAANQVQARTFHAAALRQLKYFWPQVVGEVPWRLLEA
EEEEEEHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
KFPVVAQAAHRVGLSTATESVRDLLTEIEWAKSSLVAPEDYPAAAAAHHRDIPYDAGRIA
CCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCCCCCHHH
AVYTAYESLKTTPEGLLLDFDDLLLHTAAALEDFPAVADEFRGRYRSFVVDEYQDVTPLQ
HHHHHHHHHCCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
QRVLDAWLGERDDLTVVGDANQTIYSFTGATPSYLLDFSRRFPDATVVRLERDYRSTPQV
HHHHHHHCCCCCCEEEEECCCCEEEEECCCCHHHHHHHHHHCCCCEEEEEEHHCCCCHHH
VSLANRVIGAARGRIAGTRLQLVGQRPDGPEPEFAEYDDAPAEAAAVAAKIERLVAAGTP
HHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCC
PAEIAVLYRINAQSEAYEQALAERGIPFQVRGGEGFYQRAEVRQALGALRQAASRDDLPA
CHHEEEEEEECCCHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCC
EARTGAALVRLVRAALAPVGLTATEPAGAQARERWASLTALVTLTEELVAHDAALDLTGL
HHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LRELAARAEARHPPTVQGVTLASLHAAKGLEWDAVFLVGLTDGTLPIAHVLADDGSVADV
HHHHHHHHHCCCCCCCCCCCHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHCCCCCHHHH
AALEEERRLLYVGVTRAREYLRLSWSLSRTEGGRRSRRRSRFLNGLVPDDSPASRVAAPA
HHHHHCCEEEEEEHHHHHHHHHEEEEEHHCCCHHHHHHHHHHHCCCCCCCCCHHHHHCCC
MRPSEVNRPRCRVCAKPLIGTYATMLGRCRRCPAEVDADLLAALREWRAEKAGELGVREF
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCEEE
VVFTDTTLTAIAEQLPADDRALAGIAGIGAKKIVDYGAEVLAIVASRVRSGSQNRR
EEEECHHHHHHHHHCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]