Click here to switch to the map view.

The map label for this gene is prfB

Identifier: 54026461

GI number: 54026461

Start: 4651237

End: 4652355

Strand: Reverse

Name: prfB

Synonym: nfa44880

Alternate gene names: 54026461

Gene position: 4652355-4651237 (Counterclockwise)

Preceding gene: 54026462

Following gene: 54026460

Centisome position: 77.27

GC content: 67.56

Gene sequence:

>1119_bases
GTGCATCCTGACGTTTCCGCTGATCTCGCCGAACTCGACGCCACCCTCAAGACGGTCGAGTCGGTCCTCGACATCGAGGA
ACTGCGCCGCCGCATCGATGAGCTCGAACACCAGGCCGCGGACCCGGACCTGTGGAACGACCAGGACCATGCCCAGCGCG
TGACCAGCGAACTCTCCCACGCGCAGGGCGAGTTGCGCCGGGTCGAAGACCTGCGCAGGCGGCTCGAGGACCTGCCGGTG
CTCTACGAACTCGCCGAGGGCGAGGAGGGCGAGGCCAGGACGGCCGCGCTCGAGGAGGCCGACGCCGAGCGCGCCGCGCT
GCACAGCGACGTGGAGGCGATGGAGGTCCGCACCCTGCTCTCGGGCGAGTACGACAAGCGCGAGGCGCTGGTCAACATCC
GTTCCGGCGCCGGTGGCGTGGACGCCGCGGACTGGGCCGAGATGCTGATGCGCATGTACATCCGCTGGGCCGACCGGCAC
GGCTACCCGGTGGAGGTCTACGACACCTCCTACGCCGAGGAGGCCGGGATCAAGAGCGCCACCTTCGCGGTGAAGACCCC
CTACGCCTACGGCACCCTCTCGGTCGAGATGGGCACCCACCGGCTGGTGCGGATCAGCCCGTTCGACAACCAGGGCCGCA
GGCAGACCTCCTTCGCCGAGGTCGAGGTGCTGCCGGTGGTGGAGACCACCGACCACATCGAGGTGCCCGAGACCGAGATC
CGCGTCGACGTCTACCGTTCGTCGGGTCCGGGCGGCCAGTCGGTCAACACCACCGACTCCGCGGTGCGCATCACCCACAT
CCCCACCGGCATCGTCGTGACCTGCCAGAACGAGAAATCGCAGCTGCAGAACAAGATCTCGGCCATGCGCGTGCTGCAGG
CCAAGCTGCTCGAACGCAAGCGGCAGGAGGAGCGCGCCGAGATGGACGCCCTCAAGACCAACGAGGGCGCGTCCTGGGGC
AACCAGATGCGGTCGTACGTGCTGCACCCGTACCAGATGGTCAAGGACCTGCGCACCAACTACGAGGTGAACAATCCCTC
GGCCGTGCTCAATGGTGACATCGACGGCTTCATCGAGAGCGGCATCCGCTGGCGCATGCGCGAGAGTCAGGCGAGTTAG

Upstream 100 bases:

>100_bases
GGCCATGGACACCCTGGCCGGGCGGCGTCCCGGTGGCCTGCGCGCGGTCTCCGGGTGAGCGGTTTCACCGGCTCGCGGGG
GACCGGTAGCCTTGGAGATC

Downstream 100 bases:

>100_bases
CCTGCGGCGGAGCGGCTTTCGATCGACGGGTGGATGATGTCGGTGACCTCGGATGCCCGCGTGGACGAGTCGCCGCGGCG
CGAGCGGGGTAGCCTGTCTG

Product: peptide chain release factor 2

Products: NA

Alternate protein names: RF-2

Number of amino acids: Translated: 372; Mature: 372

Protein sequence:

>372_residues
MHPDVSADLAELDATLKTVESVLDIEELRRRIDELEHQAADPDLWNDQDHAQRVTSELSHAQGELRRVEDLRRRLEDLPV
LYELAEGEEGEARTAALEEADAERAALHSDVEAMEVRTLLSGEYDKREALVNIRSGAGGVDAADWAEMLMRMYIRWADRH
GYPVEVYDTSYAEEAGIKSATFAVKTPYAYGTLSVEMGTHRLVRISPFDNQGRRQTSFAEVEVLPVVETTDHIEVPETEI
RVDVYRSSGPGGQSVNTTDSAVRITHIPTGIVVTCQNEKSQLQNKISAMRVLQAKLLERKRQEERAEMDALKTNEGASWG
NQMRSYVLHPYQMVKDLRTNYEVNNPSAVLNGDIDGFIESGIRWRMRESQAS

Sequences:

>Translated_372_residues
MHPDVSADLAELDATLKTVESVLDIEELRRRIDELEHQAADPDLWNDQDHAQRVTSELSHAQGELRRVEDLRRRLEDLPV
LYELAEGEEGEARTAALEEADAERAALHSDVEAMEVRTLLSGEYDKREALVNIRSGAGGVDAADWAEMLMRMYIRWADRH
GYPVEVYDTSYAEEAGIKSATFAVKTPYAYGTLSVEMGTHRLVRISPFDNQGRRQTSFAEVEVLPVVETTDHIEVPETEI
RVDVYRSSGPGGQSVNTTDSAVRITHIPTGIVVTCQNEKSQLQNKISAMRVLQAKLLERKRQEERAEMDALKTNEGASWG
NQMRSYVLHPYQMVKDLRTNYEVNNPSAVLNGDIDGFIESGIRWRMRESQAS
>Mature_372_residues
MHPDVSADLAELDATLKTVESVLDIEELRRRIDELEHQAADPDLWNDQDHAQRVTSELSHAQGELRRVEDLRRRLEDLPV
LYELAEGEEGEARTAALEEADAERAALHSDVEAMEVRTLLSGEYDKREALVNIRSGAGGVDAADWAEMLMRMYIRWADRH
GYPVEVYDTSYAEEAGIKSATFAVKTPYAYGTLSVEMGTHRLVRISPFDNQGRRQTSFAEVEVLPVVETTDHIEVPETEI
RVDVYRSSGPGGQSVNTTDSAVRITHIPTGIVVTCQNEKSQLQNKISAMRVLQAKLLERKRQEERAEMDALKTNEGASWG
NQMRSYVLHPYQMVKDLRTNYEVNNPSAVLNGDIDGFIESGIRWRMRESQAS

Specific function: Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family

Homologues:

Organism=Homo sapiens, GI166795303, Length=244, Percent_Identity=34.0163934426229, Blast_Score=130, Evalue=3e-30,
Organism=Homo sapiens, GI34577120, Length=237, Percent_Identity=32.4894514767932, Blast_Score=115, Evalue=7e-26,
Organism=Homo sapiens, GI166795305, Length=149, Percent_Identity=34.2281879194631, Blast_Score=91, Evalue=2e-18,
Organism=Escherichia coli, GI2367172, Length=343, Percent_Identity=44.8979591836735, Blast_Score=291, Evalue=4e-80,
Organism=Escherichia coli, GI1787462, Length=243, Percent_Identity=37.4485596707819, Blast_Score=157, Evalue=1e-39,
Organism=Caenorhabditis elegans, GI17542784, Length=213, Percent_Identity=29.1079812206573, Blast_Score=89, Evalue=5e-18,
Organism=Saccharomyces cerevisiae, GI6321295, Length=281, Percent_Identity=34.1637010676157, Blast_Score=133, Evalue=4e-32,
Organism=Drosophila melanogaster, GI19921226, Length=232, Percent_Identity=31.8965517241379, Blast_Score=129, Evalue=4e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RF2_NOCFA (Q5YR52)

Other databases:

- EMBL:   AP006618
- RefSeq:   YP_120703.1
- ProteinModelPortal:   Q5YR52
- SMR:   Q5YR52
- GeneID:   3111389
- GenomeReviews:   AP006618_GR
- KEGG:   nfa:nfa44880
- NMPDR:   fig|247156.1.peg.4314
- HOGENOM:   HBG629764
- OMA:   TEMLLRM
- ProtClustDB:   PRK00578
- BioCyc:   NFAR247156:NFA44880-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00094
- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR020853
- InterPro:   IPR004374
- PANTHER:   PTHR11075:SF6
- SMART:   SM00937
- TIGRFAMs:   TIGR00020

Pfam domain/function: PF03462 PCRF; PF00472 RF-1

EC number: NA

Molecular weight: Translated: 41970; Mature: 41970

Theoretical pI: Translated: 4.58; Mature: 4.58

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHPDVSADLAELDATLKTVESVLDIEELRRRIDELEHQAADPDLWNDQDHAQRVTSELSH
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHH
AQGELRRVEDLRRRLEDLPVLYELAEGEEGEARTAALEEADAERAALHSDVEAMEVRTLL
HHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
SGEYDKREALVNIRSGAGGVDAADWAEMLMRMYIRWADRHGYPVEVYDTSYAEEAGIKSA
CCCCCCHHHHEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHCCCCCE
TFAVKTPYAYGTLSVEMGTHRLVRISPFDNQGRRQTSFAEVEVLPVVETTDHIEVPETEI
EEEEECCCEEEEEEEEECCCEEEEECCCCCCCCCCCCCEEEEEEEEEECCCCCCCCCCEE
RVDVYRSSGPGGQSVNTTDSAVRITHIPTGIVVTCQNEKSQLQNKISAMRVLQAKLLERK
EEEEEECCCCCCCCCCCCCCEEEEEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHH
RQEERAEMDALKTNEGASWGNQMRSYVLHPYQMVKDLRTNYEVNNPSAVLNGDIDGFIES
HHHHHHHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHHHHCCCCCCCCEEECCCHHHHHHC
GIRWRMRESQAS
CHHEEHHHCCCC
>Mature Secondary Structure
MHPDVSADLAELDATLKTVESVLDIEELRRRIDELEHQAADPDLWNDQDHAQRVTSELSH
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHH
AQGELRRVEDLRRRLEDLPVLYELAEGEEGEARTAALEEADAERAALHSDVEAMEVRTLL
HHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
SGEYDKREALVNIRSGAGGVDAADWAEMLMRMYIRWADRHGYPVEVYDTSYAEEAGIKSA
CCCCCCHHHHEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHCCCCCE
TFAVKTPYAYGTLSVEMGTHRLVRISPFDNQGRRQTSFAEVEVLPVVETTDHIEVPETEI
EEEEECCCEEEEEEEEECCCEEEEECCCCCCCCCCCCCEEEEEEEEEECCCCCCCCCCEE
RVDVYRSSGPGGQSVNTTDSAVRITHIPTGIVVTCQNEKSQLQNKISAMRVLQAKLLERK
EEEEEECCCCCCCCCCCCCCEEEEEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHH
RQEERAEMDALKTNEGASWGNQMRSYVLHPYQMVKDLRTNYEVNNPSAVLNGDIDGFIES
HHHHHHHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHHHHCCCCCCCCEEECCCHHHHHHC
GIRWRMRESQAS
CHHEEHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA