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The map label for this gene is ddl

Identifier: 54026176

GI number: 54026176

Start: 4354408

End: 4355499

Strand: Reverse

Name: ddl

Synonym: nfa42050

Alternate gene names: 54026176

Gene position: 4355499-4354408 (Counterclockwise)

Preceding gene: 54026177

Following gene: 54026172

Centisome position: 72.34

GC content: 70.15

Gene sequence:

>1092_bases
ATGACGAACCGGATCAGGGTGGCTGTGGTGTTCGGTGGGCGGAGCAACGAGCACGCGGTGTCGTGTGTCTCGGCCGGGAG
CGTGCTGCGGCACCTCGATCCCACCAGATACGAGGCCGTCCCCATCGGCATCACCCCGCAGGGCGGCTGGGTGCTGGCGA
ATTCCGACGTCGCCGCGATCGGCCTGAGCCACGAGGCGCTGCCCTCGGTGGACGCCGGCGGCACCGCCCTGACCCTGACC
GCCGACCCCACCCGCGGTGGCGAGCTGGTCGCGCTCGACGACGCGGGCGCGGTGCTCGGGACGGTGGACGTGGTGTTCCC
GATCCTGCACGGTCCCTTCGGTGAGGACGGCACGCTCCAGGGCATGCTCGAACTCGCCGGGGTGCCCTATGTCGGGCCCG
GCGTGTTGGCCAGCGCCGCGGGCATGGACAAGGAGTTCACCAAGAAACTGCTCGCCGCCGAGGGGCTTCCGGTCGGCACC
CAGGTGGTGCTGCGGCCCGGGACCGCGACCCTGACCGAGGAACAGCGCGAGCGGCTCGGACTGCCGGTGTTCGTGAAACC
GGCGCGGGCGGGTTCCTCGATCGGCATCACCAAGGTCGACGACTGGGCGGCGCTGGACACCGCGATCGCGGCGGCGCGCG
AACACGATCCGAAGGTGATCGTGGAGGCGGGCATCGTCGGCCGCGAGGTGGAATGCGGCGTGCTGGAATTCCCGGACGGC
CGGATCTCGGCCAGCGTGCTCGCCGAGATCCGGATGCCCGAGGGCGACGGGCCGCAGTTCTACGACTTCGACACCAAGTA
CCTCGACGACGTGTGCGAATTCGACGTCCCGGCCAAGCTCGACGACGACATCGCCGAGCAGGTCCGGGAACTGGCCGTGC
GGGCCTTCCAGGCCCTGGACTGCCAGGGCCTGGCCCGCGTGGACTTCTTCGTCACCGCCGACGGCCCGGTCATCAACGAG
ATCAACACCATGCCGGGCTTCACCTCGATCTCGATGTACCCGCGCATGTGGGAGGCCACCGGCATCGACTACGGGACACT
GGTGTCGACGCTGATCGAGACGGCGCTGGCGCGGGGGACCGGGCTGCGGTAG

Upstream 100 bases:

>100_bases
GCAGCCTGCTCGGACGCCGCATCAAGCCGGAGTAGGGACCCCAGAAGTCCGGGCACGGGCAGCGACGGAGAAGCCCGCCG
CGCCCGGTACCGTTCGGTGC

Downstream 100 bases:

>100_bases
CGGACGGGGTCACACCGCGCGGGCGGCGCGGGCGCACGCGGCGACGGCCGCGCGGGCGGCGTGTAGTGCGCTGTTGATCG
AGATCTCGCCGCGCATCCCG

Product: D-alanyl-alanine synthetase A

Products: NA

Alternate protein names: D-Ala-D-Ala ligase; D-alanylalanine synthetase

Number of amino acids: Translated: 363; Mature: 362

Protein sequence:

>363_residues
MTNRIRVAVVFGGRSNEHAVSCVSAGSVLRHLDPTRYEAVPIGITPQGGWVLANSDVAAIGLSHEALPSVDAGGTALTLT
ADPTRGGELVALDDAGAVLGTVDVVFPILHGPFGEDGTLQGMLELAGVPYVGPGVLASAAGMDKEFTKKLLAAEGLPVGT
QVVLRPGTATLTEEQRERLGLPVFVKPARAGSSIGITKVDDWAALDTAIAAAREHDPKVIVEAGIVGREVECGVLEFPDG
RISASVLAEIRMPEGDGPQFYDFDTKYLDDVCEFDVPAKLDDDIAEQVRELAVRAFQALDCQGLARVDFFVTADGPVINE
INTMPGFTSISMYPRMWEATGIDYGTLVSTLIETALARGTGLR

Sequences:

>Translated_363_residues
MTNRIRVAVVFGGRSNEHAVSCVSAGSVLRHLDPTRYEAVPIGITPQGGWVLANSDVAAIGLSHEALPSVDAGGTALTLT
ADPTRGGELVALDDAGAVLGTVDVVFPILHGPFGEDGTLQGMLELAGVPYVGPGVLASAAGMDKEFTKKLLAAEGLPVGT
QVVLRPGTATLTEEQRERLGLPVFVKPARAGSSIGITKVDDWAALDTAIAAAREHDPKVIVEAGIVGREVECGVLEFPDG
RISASVLAEIRMPEGDGPQFYDFDTKYLDDVCEFDVPAKLDDDIAEQVRELAVRAFQALDCQGLARVDFFVTADGPVINE
INTMPGFTSISMYPRMWEATGIDYGTLVSTLIETALARGTGLR
>Mature_362_residues
TNRIRVAVVFGGRSNEHAVSCVSAGSVLRHLDPTRYEAVPIGITPQGGWVLANSDVAAIGLSHEALPSVDAGGTALTLTA
DPTRGGELVALDDAGAVLGTVDVVFPILHGPFGEDGTLQGMLELAGVPYVGPGVLASAAGMDKEFTKKLLAAEGLPVGTQ
VVLRPGTATLTEEQRERLGLPVFVKPARAGSSIGITKVDDWAALDTAIAAAREHDPKVIVEAGIVGREVECGVLEFPDGR
ISASVLAEIRMPEGDGPQFYDFDTKYLDDVCEFDVPAKLDDDIAEQVRELAVRAFQALDCQGLARVDFFVTADGPVINEI
NTMPGFTSISMYPRMWEATGIDYGTLVSTLIETALARGTGLR

Specific function: Cell wall formation

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Escherichia coli, GI1786579, Length=359, Percent_Identity=44.2896935933148, Blast_Score=277, Evalue=8e-76,
Organism=Escherichia coli, GI1786280, Length=258, Percent_Identity=39.5348837209302, Blast_Score=163, Evalue=1e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DDL_NOCFA (Q5YRY7)

Other databases:

- EMBL:   AP006618
- RefSeq:   YP_120418.1
- HSSP:   P07862
- ProteinModelPortal:   Q5YRY7
- SMR:   Q5YRY7
- GeneID:   3109811
- GenomeReviews:   AP006618_GR
- KEGG:   nfa:nfa42050
- NMPDR:   fig|247156.1.peg.4054
- HOGENOM:   HBG427092
- OMA:   FTDISMY
- ProtClustDB:   PRK01966
- BioCyc:   NFAR247156:NFA42050-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00047
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.40.50.20
- TIGRFAMs:   TIGR01205

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N; SSF52440 PreATP-grasp-like

EC number: =6.3.2.4

Molecular weight: Translated: 38229; Mature: 38098

Theoretical pI: Translated: 4.27; Mature: 4.27

Prosite motif: PS50975 ATP_GRASP; PS00843 DALA_DALA_LIGASE_1; PS00844 DALA_DALA_LIGASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNRIRVAVVFGGRSNEHAVSCVSAGSVLRHLDPTRYEAVPIGITPQGGWVLANSDVAAI
CCCEEEEEEEECCCCCCCEEEHHHHHHHHHHCCCCCEEEEEEEECCCCCEEEECCCEEEE
GLSHEALPSVDAGGTALTLTADPTRGGELVALDDAGAVLGTVDVVFPILHGPFGEDGTLQ
ECCCHHCCCCCCCCEEEEEEECCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCCCHHH
GMLELAGVPYVGPGVLASAAGMDKEFTKKLLAAEGLPVGTQVVLRPGTATLTEEQRERLG
HHHHHCCCCCCCCHHHHHHCCCCHHHHHHHHHHCCCCCCCEEEEECCCCCCCHHHHHHCC
LPVFVKPARAGSSIGITKVDDWAALDTAIAAAREHDPKVIVEAGIVGREVECGVLEFPDG
CCEEEECCCCCCCCCCEECCCHHHHHHHHHHHHCCCCEEEEEECCCCCEECCCEEECCCC
RISASVLAEIRMPEGDGPQFYDFDTKYLDDVCEFDVPAKLDDDIAEQVRELAVRAFQALD
CCCCEEEEEEECCCCCCCEEECCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCC
CQGLARVDFFVTADGPVINEINTMPGFTSISMYPRMWEATGIDYGTLVSTLIETALARGT
CCCCEEEEEEEECCCCEECCCCCCCCCCEEECCCCHHHHCCCCHHHHHHHHHHHHHHCCC
GLR
CCC
>Mature Secondary Structure 
TNRIRVAVVFGGRSNEHAVSCVSAGSVLRHLDPTRYEAVPIGITPQGGWVLANSDVAAI
CCEEEEEEEECCCCCCCEEEHHHHHHHHHHCCCCCEEEEEEEECCCCCEEEECCCEEEE
GLSHEALPSVDAGGTALTLTADPTRGGELVALDDAGAVLGTVDVVFPILHGPFGEDGTLQ
ECCCHHCCCCCCCCEEEEEEECCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCCCHHH
GMLELAGVPYVGPGVLASAAGMDKEFTKKLLAAEGLPVGTQVVLRPGTATLTEEQRERLG
HHHHHCCCCCCCCHHHHHHCCCCHHHHHHHHHHCCCCCCCEEEEECCCCCCCHHHHHHCC
LPVFVKPARAGSSIGITKVDDWAALDTAIAAAREHDPKVIVEAGIVGREVECGVLEFPDG
CCEEEECCCCCCCCCCEECCCHHHHHHHHHHHHCCCCEEEEEECCCCCEECCCEEECCCC
RISASVLAEIRMPEGDGPQFYDFDTKYLDDVCEFDVPAKLDDDIAEQVRELAVRAFQALD
CCCCEEEEEEECCCCCCCEEECCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCC
CQGLARVDFFVTADGPVINEINTMPGFTSISMYPRMWEATGIDYGTLVSTLIETALARGT
CCCCEEEEEEEECCCCEECCCCCCCCCCEEECCCCHHHHCCCCHHHHHHHHHHHHHHCCC
GLR
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA