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The map label for this gene is amt [H]

Identifier: 54026135

GI number: 54026135

Start: 4311614

End: 4312960

Strand: Reverse

Name: amt [H]

Synonym: nfa41640

Alternate gene names: 54026135

Gene position: 4312960-4311614 (Counterclockwise)

Preceding gene: 54026136

Following gene: 54026134

Centisome position: 71.63

GC content: 67.63

Gene sequence:

>1347_bases
GTGGCTTTTCCCGTAATCGGCGCACCGGACGCCGGTGACACCGCATGGATGCTGGCCAGTGCCGCACTCGTGCTGTTGAT
GACGCCAGGACTCGCGTTCTTCTACGGCGGCATGGTCCGCAGCAAGAACGTGCTCAACATGATCATGATGAGCATCAGCG
CGATGGGTTTGATCACCATCCTCTGGGTGCTGTACGGCTTCTCCGTCGCCTTCGGTGAGGACAAGGCCAACCTCATCGGC
GACCCCGGCCAGTACTTCGGCCTGAAGGGGGTGTTCGGCGGCAGCTACCTGGCCTCCACCGATCCCGCCGAGCCCGCCGC
GATCCCGCTCAACGGCACCATCCCGCTGTCGCTGTTCGTCGCGTTCCAGCTCATGTTCGCGATCATCACCGTCGCACTCA
TCTCGGGCGCGGTCGCCGACCGCCTGAAGTTCGGCTCGTGGCTGGTCTTCGCGGGCGTGTGGGCCACCATCGTCTACTTC
CCGGTGGCGCACTGGGTGTTCGCCTTCGACGGGTACACCGGCGAGGAGGGCGGCTGGATCGCCAACAACCTCAAGGCCAT
CGACTTCGCGGGCGGCACCGCGGTGCACATCAACGCCGGCGCCGCCGGTCTGGTGCTCGCGCTGATCCTCGGGAAGCGCA
AGGGCTGGCCGCAGACGCCGTTCCGCCCGCACAACCTGCCCTTCGTCATGCTCGGCGCCGGTCTGCTCTGGTTCGGCTGG
TACGGCTTCAACGCCGGGTCGGCCGTCGGCTCCAACGGGATCGCCGGCGCGACGTTCCTCACCACCACCATCGCCACCGC
CGCCGCGATGCTCGCCTGGCTGGTGGTGGAGAAGATCCGCGACGGCCACCCCACCTCGCTGGGCGCGGCGTCGGGCATCG
TGGCAGGCCTGGTCGCCATCACCCCGGCGTGCTCCTCGGTGAACGTGCTCGGTGCGCTGGCCATCGGTGTGGTCTCCGGT
GCGCTGTGTGCGCTGGCGGTCGGCCTGAAGTTCAGGTTCGGCTTCGACGACTCGCTCGACGTGGTCGGCGTGCACCTGGT
CGGCGGCCTGGTCGGCACCCTGATGGTCGGCCTGGTGGCGGCGCCGGAAGCGCCCGCGGCGGTCGAGGGCTTGTTCTACG
GTGGTGGGATCGAGCAGTTGAAGCTGCAGGCCGTCGGTGCGTTCACCGTGCTTGCCTACTCGCTGGTCGCGACGGCCGTC
ATTGCTCTGATCCTGAAGTTCACGATCGGTCTGCGCGCCGACGACGAGGCCGAGTCGGTGGGTCTGGACGAGTCCGAGCA
CGCGGAAACCGCATACGATTTCGCTGCTGTGGGTGGCACGGCACGCTCGCTCGTCAAGGAGGCATGA

Upstream 100 bases:

>100_bases
TGAAACACCAAAGCTCTCCGGATGAAACACCGAGCGAACACCCTTCTCTGCAGGTCCATTTGCCACAGGCGGCTGGGCCC
GAGATGAGGAGGACATTAAG

Downstream 100 bases:

>100_bases
CGACATGAAACTGATCACCGCAATCGTCAAACCGTTCACGCTCGAGGACGTCAAGACCGGTCTGGAACAGGCGGGCGTGT
TCGGCATGACCGTCAGCGAG

Product: putative ammonium transporter

Products: NH3 [Cytoplasm] [C]

Alternate protein names: Ammonia transporter [H]

Number of amino acids: Translated: 448; Mature: 447

Protein sequence:

>448_residues
MAFPVIGAPDAGDTAWMLASAALVLLMTPGLAFFYGGMVRSKNVLNMIMMSISAMGLITILWVLYGFSVAFGEDKANLIG
DPGQYFGLKGVFGGSYLASTDPAEPAAIPLNGTIPLSLFVAFQLMFAIITVALISGAVADRLKFGSWLVFAGVWATIVYF
PVAHWVFAFDGYTGEEGGWIANNLKAIDFAGGTAVHINAGAAGLVLALILGKRKGWPQTPFRPHNLPFVMLGAGLLWFGW
YGFNAGSAVGSNGIAGATFLTTTIATAAAMLAWLVVEKIRDGHPTSLGAASGIVAGLVAITPACSSVNVLGALAIGVVSG
ALCALAVGLKFRFGFDDSLDVVGVHLVGGLVGTLMVGLVAAPEAPAAVEGLFYGGGIEQLKLQAVGAFTVLAYSLVATAV
IALILKFTIGLRADDEAESVGLDESEHAETAYDFAAVGGTARSLVKEA

Sequences:

>Translated_448_residues
MAFPVIGAPDAGDTAWMLASAALVLLMTPGLAFFYGGMVRSKNVLNMIMMSISAMGLITILWVLYGFSVAFGEDKANLIG
DPGQYFGLKGVFGGSYLASTDPAEPAAIPLNGTIPLSLFVAFQLMFAIITVALISGAVADRLKFGSWLVFAGVWATIVYF
PVAHWVFAFDGYTGEEGGWIANNLKAIDFAGGTAVHINAGAAGLVLALILGKRKGWPQTPFRPHNLPFVMLGAGLLWFGW
YGFNAGSAVGSNGIAGATFLTTTIATAAAMLAWLVVEKIRDGHPTSLGAASGIVAGLVAITPACSSVNVLGALAIGVVSG
ALCALAVGLKFRFGFDDSLDVVGVHLVGGLVGTLMVGLVAAPEAPAAVEGLFYGGGIEQLKLQAVGAFTVLAYSLVATAV
IALILKFTIGLRADDEAESVGLDESEHAETAYDFAAVGGTARSLVKEA
>Mature_447_residues
AFPVIGAPDAGDTAWMLASAALVLLMTPGLAFFYGGMVRSKNVLNMIMMSISAMGLITILWVLYGFSVAFGEDKANLIGD
PGQYFGLKGVFGGSYLASTDPAEPAAIPLNGTIPLSLFVAFQLMFAIITVALISGAVADRLKFGSWLVFAGVWATIVYFP
VAHWVFAFDGYTGEEGGWIANNLKAIDFAGGTAVHINAGAAGLVLALILGKRKGWPQTPFRPHNLPFVMLGAGLLWFGWY
GFNAGSAVGSNGIAGATFLTTTIATAAAMLAWLVVEKIRDGHPTSLGAASGIVAGLVAITPACSSVNVLGALAIGVVSGA
LCALAVGLKFRFGFDDSLDVVGVHLVGGLVGTLMVGLVAAPEAPAAVEGLFYGGGIEQLKLQAVGAFTVLAYSLVATAVI
ALILKFTIGLRADDEAESVGLDESEHAETAYDFAAVGGTARSLVKEA

Specific function: Involved in the uptake of ammonia (Probable) [H]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=441, Percent_Identity=43.7641723356009, Blast_Score=289, Evalue=3e-79,
Organism=Caenorhabditis elegans, GI17550180, Length=456, Percent_Identity=28.2894736842105, Blast_Score=130, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI17550182, Length=451, Percent_Identity=28.3813747228381, Blast_Score=130, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI133931100, Length=436, Percent_Identity=25.9174311926606, Blast_Score=122, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI32564042, Length=433, Percent_Identity=27.4826789838337, Blast_Score=117, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6325396, Length=437, Percent_Identity=33.1807780320366, Blast_Score=233, Evalue=7e-62,
Organism=Saccharomyces cerevisiae, GI6324187, Length=441, Percent_Identity=34.0136054421769, Blast_Score=228, Evalue=1e-60,
Organism=Saccharomyces cerevisiae, GI6321559, Length=437, Percent_Identity=31.5789473684211, Blast_Score=218, Evalue=2e-57,
Organism=Drosophila melanogaster, GI161078314, Length=435, Percent_Identity=28.9655172413793, Blast_Score=131, Evalue=1e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 46076; Mature: 45945

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAFPVIGAPDAGDTAWMLASAALVLLMTPGLAFFYGGMVRSKNVLNMIMMSISAMGLITI
CCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
LWVLYGFSVAFGEDKANLIGDPGQYFGLKGVFGGSYLASTDPAEPAAIPLNGTIPLSLFV
HHHHHHCHHEECCCCCCCCCCCCHHCCCEECCCCCCCCCCCCCCCCEECCCCCCHHHHHH
AFQLMFAIITVALISGAVADRLKFGSWLVFAGVWATIVYFPVAHWVFAFDGYTGEEGGWI
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEE
ANNLKAIDFAGGTAVHINAGAAGLVLALILGKRKGWPQTPFRPHNLPFVMLGAGLLWFGW
ECCCEEEEECCCCEEEEECCHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
YGFNAGSAVGSNGIAGATFLTTTIATAAAMLAWLVVEKIRDGHPTSLGAASGIVAGLVAI
HCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
TPACSSVNVLGALAIGVVSGALCALAVGLKFRFGFDDSLDVVGVHLVGGLVGTLMVGLVA
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHHHHHHHHHHHC
APEAPAAVEGLFYGGGIEQLKLQAVGAFTVLAYSLVATAVIALILKFTIGLRADDEAESV
CCCCCHHHHHEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHC
GLDESEHAETAYDFAAVGGTARSLVKEA
CCCCCHHHHHHHHHHHHCHHHHHHHHCC
>Mature Secondary Structure 
AFPVIGAPDAGDTAWMLASAALVLLMTPGLAFFYGGMVRSKNVLNMIMMSISAMGLITI
CCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
LWVLYGFSVAFGEDKANLIGDPGQYFGLKGVFGGSYLASTDPAEPAAIPLNGTIPLSLFV
HHHHHHCHHEECCCCCCCCCCCCHHCCCEECCCCCCCCCCCCCCCCEECCCCCCHHHHHH
AFQLMFAIITVALISGAVADRLKFGSWLVFAGVWATIVYFPVAHWVFAFDGYTGEEGGWI
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEE
ANNLKAIDFAGGTAVHINAGAAGLVLALILGKRKGWPQTPFRPHNLPFVMLGAGLLWFGW
ECCCEEEEECCCCEEEEECCHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
YGFNAGSAVGSNGIAGATFLTTTIATAAAMLAWLVVEKIRDGHPTSLGAASGIVAGLVAI
HCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
TPACSSVNVLGALAIGVVSGALCALAVGLKFRFGFDDSLDVVGVHLVGGLVGTLMVGLVA
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHHHHHHHHHHHC
APEAPAAVEGLFYGGGIEQLKLQAVGAFTVLAYSLVATAVIALILKFTIGLRADDEAESV
CCCCCHHHHHEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHC
GLDESEHAETAYDFAAVGGTARSLVKEA
CCCCCHHHHHHHHHHHHCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]