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The map label for this gene is lip2 [H]

Identifier: 54026067

GI number: 54026067

Start: 4247452

End: 4248426

Strand: Reverse

Name: lip2 [H]

Synonym: nfa40960

Alternate gene names: 54026067

Gene position: 4248426-4247452 (Counterclockwise)

Preceding gene: 54026068

Following gene: 54026066

Centisome position: 70.56

GC content: 73.23

Gene sequence:

>975_bases
GTGACCGAGATGGGCGCACAGCGACGGTCCGATCTGCACCCCGACGTGGCGGCGATGCTCGCGGCACTGGAGTCGTTCCC
GGATGTCACCCGCTACGAGGCCGCCGAACTCCGCGAGATCATCGCCGCCCGGCGCGCGCCGCTGACCCGTCAGCCGGACA
TGCGGACGGCGCGGGATCACGTGATACCCGGTCCGGGCGGGGATCTCACGGTGCGGGTGTACGTCCCGCACGGTGACGCC
CTGCGGCCGGTGGTGGTGTTCGCCCACGGCGGCGGATTCGTCTTCTGCGATCTGGACAGCCACGACGAGTTCTGCCGCAG
CACCGCGCAGGCGGTGGACGCGGTGGTCGTCTCGGTCGACTACCGCCGCGCGCCCGAGCACCCCGGTCCGGCGGCGATGG
AGGACCTCTACGCCGCGGTGACCTGGGTGCACCGGCACGCGGGCGAGTTCGGCGGCGACCCGGAGCGGATCGCGGTGGCC
GGTGACAGCGCGGGCGGGAACCTCGCCGCCACGGTGAGCCTGGCCGCGCGCGACCGGGGCGGCCCGCCGATCGCGGCGCA
GGTGCTGATCTATCCGGTGATCGACGACGACTTCGACACCGAGTCCTACCGCCGTTACGGCGCGGGGTACTACAACACCA
CCGAGGCCATGCGGTGGTACTGGCAGCAGTACGCCCCGCACGGCACCGACAGTCCGTATCTGGTGCCCACCAGGGCCGAC
AGCCTCGCCGGGCTGCCGCCCGCGGTGGTCGTCACCGCCGAACTCGACCCGCCCTGCTCGGCGGGGGAGGACTACGCCCG
GCGGCTGGCAGCGGCGGGCGTGCCGGTCACCGCGCACCGCTTCGACGGTCTGTTCCACGGGTTCCTGACCTTCCCGAAGC
TCTCGCTCACCGGACCCGCCCGCGCGGAACTCTGGCAGATGATGCGCGCGGTCCTCGCCTCGCCGCGCCCCGCGGTCGGC
GAGGGCACGGCATGA

Upstream 100 bases:

>100_bases
CATCGGCGGGTTCGGCAACTACAGCCAGATCTGCGACGAGGTGGCCGCCGCCGGGTACAAGGGCTTCGTCCTCGACGGCG
CCGCGGCGGACGAGTGAGGG

Downstream 100 bases:

>100_bases
GCCGGCGGTCGACGGGCAGGCCGCGCGACACCCACGCCCGCACGGGCGCCGCACTCCTGGAAGGTGACCCATGACCGACA
CCCTCACCGGCACTGACCTG

Product: putative esterase

Products: NA

Alternate protein names: Triacylglycerol lipase [H]

Number of amino acids: Translated: 324; Mature: 323

Protein sequence:

>324_residues
MTEMGAQRRSDLHPDVAAMLAALESFPDVTRYEAAELREIIAARRAPLTRQPDMRTARDHVIPGPGGDLTVRVYVPHGDA
LRPVVVFAHGGGFVFCDLDSHDEFCRSTAQAVDAVVVSVDYRRAPEHPGPAAMEDLYAAVTWVHRHAGEFGGDPERIAVA
GDSAGGNLAATVSLAARDRGGPPIAAQVLIYPVIDDDFDTESYRRYGAGYYNTTEAMRWYWQQYAPHGTDSPYLVPTRAD
SLAGLPPAVVVTAELDPPCSAGEDYARRLAAAGVPVTAHRFDGLFHGFLTFPKLSLTGPARAELWQMMRAVLASPRPAVG
EGTA

Sequences:

>Translated_324_residues
MTEMGAQRRSDLHPDVAAMLAALESFPDVTRYEAAELREIIAARRAPLTRQPDMRTARDHVIPGPGGDLTVRVYVPHGDA
LRPVVVFAHGGGFVFCDLDSHDEFCRSTAQAVDAVVVSVDYRRAPEHPGPAAMEDLYAAVTWVHRHAGEFGGDPERIAVA
GDSAGGNLAATVSLAARDRGGPPIAAQVLIYPVIDDDFDTESYRRYGAGYYNTTEAMRWYWQQYAPHGTDSPYLVPTRAD
SLAGLPPAVVVTAELDPPCSAGEDYARRLAAAGVPVTAHRFDGLFHGFLTFPKLSLTGPARAELWQMMRAVLASPRPAVG
EGTA
>Mature_323_residues
TEMGAQRRSDLHPDVAAMLAALESFPDVTRYEAAELREIIAARRAPLTRQPDMRTARDHVIPGPGGDLTVRVYVPHGDAL
RPVVVFAHGGGFVFCDLDSHDEFCRSTAQAVDAVVVSVDYRRAPEHPGPAAMEDLYAAVTWVHRHAGEFGGDPERIAVAG
DSAGGNLAATVSLAARDRGGPPIAAQVLIYPVIDDDFDTESYRRYGAGYYNTTEAMRWYWQQYAPHGTDSPYLVPTRADS
LAGLPPAVVVTAELDPPCSAGEDYARRLAAAGVPVTAHRFDGLFHGFLTFPKLSLTGPARAELWQMMRAVLASPRPAVGE
GTA

Specific function: Has An Esterase Activity. Triacetyl Glycerol (Triacetin) Is A Substrate Of The Enzyme. [C]

COG id: COG0657

COG function: function code I; Esterase/lipase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDXG' lipolytic enzyme family [H]

Homologues:

Organism=Homo sapiens, GI68299767, Length=167, Percent_Identity=38.3233532934132, Blast_Score=104, Evalue=1e-22,
Organism=Homo sapiens, GI206597554, Length=312, Percent_Identity=28.8461538461538, Blast_Score=99, Evalue=7e-21,
Organism=Homo sapiens, GI157041239, Length=287, Percent_Identity=27.5261324041812, Blast_Score=95, Evalue=1e-19,
Organism=Homo sapiens, GI68051721, Length=279, Percent_Identity=27.9569892473118, Blast_Score=92, Evalue=7e-19,
Organism=Homo sapiens, GI21328446, Length=95, Percent_Identity=46.3157894736842, Blast_Score=90, Evalue=3e-18,
Organism=Homo sapiens, GI226423947, Length=287, Percent_Identity=27.1777003484321, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI61966717, Length=132, Percent_Identity=35.6060606060606, Blast_Score=86, Evalue=6e-17,
Organism=Homo sapiens, GI157041237, Length=265, Percent_Identity=26.4150943396226, Blast_Score=80, Evalue=4e-15,
Organism=Escherichia coli, GI1786682, Length=253, Percent_Identity=30.0395256916996, Blast_Score=108, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI17567059, Length=277, Percent_Identity=27.4368231046931, Blast_Score=94, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI71996133, Length=178, Percent_Identity=35.3932584269663, Blast_Score=93, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI72001146, Length=125, Percent_Identity=36.8, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17540028, Length=178, Percent_Identity=34.8314606741573, Blast_Score=90, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17562530, Length=151, Percent_Identity=31.7880794701987, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI115533410, Length=91, Percent_Identity=39.5604395604396, Blast_Score=75, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI115533412, Length=91, Percent_Identity=39.5604395604396, Blast_Score=75, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI86563868, Length=146, Percent_Identity=31.5068493150685, Blast_Score=71, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI17559616, Length=135, Percent_Identity=34.0740740740741, Blast_Score=71, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24656084, Length=99, Percent_Identity=41.4141414141414, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24656076, Length=99, Percent_Identity=41.4141414141414, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI20130169, Length=99, Percent_Identity=41.4141414141414, Blast_Score=80, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013094
- InterPro:   IPR002168 [H]

Pfam domain/function: PF07859 Abhydrolase_3 [H]

EC number: =3.1.1.3 [H]

Molecular weight: Translated: 34936; Mature: 34804

Theoretical pI: Translated: 5.35; Mature: 5.35

Prosite motif: PS01174 LIPASE_GDXG_SER ; PS00122 CARBOXYLESTERASE_B_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEMGAQRRSDLHPDVAAMLAALESFPDVTRYEAAELREIIAARRAPLTRQPDMRTARDH
CCCCCCHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCC
VIPGPGGDLTVRVYVPHGDALRPVVVFAHGGGFVFCDLDSHDEFCRSTAQAVDAVVVSVD
CCCCCCCCEEEEEEECCCCCCCEEEEEEECCCEEEEECCCCHHHHHHHHHHHHEEEEEEE
YRRAPEHPGPAAMEDLYAAVTWVHRHAGEFGGDPERIAVAGDSAGGNLAATVSLAARDRG
CCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCEEEEEEEEECCCC
GPPIAAQVLIYPVIDDDFDTESYRRYGAGYYNTTEAMRWYWQQYAPHGTDSPYLVPTRAD
CCCEEEEEEEEEEECCCCCHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCEEEECCCC
SLAGLPPAVVVTAELDPPCSAGEDYARRLAAAGVPVTAHRFDGLFHGFLTFPKLSLTGPA
CCCCCCCEEEEEEECCCCCCCCHHHHHHHHHCCCCEEHHHHHHHHHHHHHCCCEECCCCH
RAELWQMMRAVLASPRPAVGEGTA
HHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
TEMGAQRRSDLHPDVAAMLAALESFPDVTRYEAAELREIIAARRAPLTRQPDMRTARDH
CCCCCHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCC
VIPGPGGDLTVRVYVPHGDALRPVVVFAHGGGFVFCDLDSHDEFCRSTAQAVDAVVVSVD
CCCCCCCCEEEEEEECCCCCCCEEEEEEECCCEEEEECCCCHHHHHHHHHHHHEEEEEEE
YRRAPEHPGPAAMEDLYAAVTWVHRHAGEFGGDPERIAVAGDSAGGNLAATVSLAARDRG
CCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCEEEEEEEEECCCC
GPPIAAQVLIYPVIDDDFDTESYRRYGAGYYNTTEAMRWYWQQYAPHGTDSPYLVPTRAD
CCCEEEEEEEEEEECCCCCHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCEEEECCCC
SLAGLPPAVVVTAELDPPCSAGEDYARRLAAAGVPVTAHRFDGLFHGFLTFPKLSLTGPA
CCCCCCCEEEEEEECCCCCCCCHHHHHHHHHCCCCEEHHHHHHHHHHHHHCCCEECCCCH
RAELWQMMRAVLASPRPAVGEGTA
HHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1907455 [H]