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The map label for this gene is efpA [H]

Identifier: 54026047

GI number: 54026047

Start: 4225693

End: 4227177

Strand: Reverse

Name: efpA [H]

Synonym: nfa40760

Alternate gene names: 54026047

Gene position: 4227177-4225693 (Counterclockwise)

Preceding gene: 54026048

Following gene: 54026044

Centisome position: 70.2

GC content: 71.18

Gene sequence:

>1485_bases
GTGCTCGAGAACCCCGCTGCGACGACGCAGTTTCCGGTGACGCAGCGCGCCTTCGGGCTCGCCATCCTCGTGTTGAGCGG
GCTACAGCTGATGGTCGTGCTCGACGGCACGGTGGTGATCTTCGCGCTGCCTCGGCTGCAGGACCAGATGGGGCTGTCCA
GCGCGGGCAGCGCCTGGATCGTCACCGCCTACGGCCTGACCTTCGCCGGGCTGATGCTGCTCGGCGGGCGTCTCGGCGAC
GCGTTCGGCCGCAAACGCATGCTCATCGCCGGCGTCGGGCTGTTCACGGTCGCCTCGCTGCTGTGCGGCCTCGCCCACTG
GCAAGCGATGCTCATCGCGGCGCGCGCGCTGCAGGGCGCGGGCGCGGCCATCGCGGCCCCCGTCGCCTTCGCGCTGGTCG
CCACCACCTTCGCACCCGGCAAGGCCCGCAACCAGGCGATCGCCATCGTCGGCTCGATGGTCGGCATCGGTTCGGTGGGC
GGGCTGGTGGTCGGCGGCGCGCTGACCCAGCTGTCGTGGCGCTGGATCTTCCTGATCAACGTGCCGATCGGCGCCCTGAT
CATCCTCGGCGCGATCTACTGCCTGGCCGACACCGGCCACCACCGGGTCGCCCTCGACGCGCGCGGCGCCGTGCTCGGCA
CGCTGGCCTGCGCGGCGATCGTCTTCGGCGCCACCGAGGGCCCGGAGCTGGGCTGGTCGCATCCGGCCGTGATCGGCGCG
CTGATCGGCGGCGCGATCCTGCTCGTGGTCTTCGTGATCGCCGAACGCAACGTCGACGATCCGCTGCTGCCCTGGTCGCT
GTTCGACAGCCGCGACCGGGTCACCACCTTCGTGCTGATCCTGCTCGCCGGCGGTGTGCTCGGCGCGATGACCTACTTCG
TCGCCCAGTTCCTGCAGAACGTGCTCGGCTACGGTCCGCTGCAAGCGGGCGTGGCCTCCATCCCGTTCACCGTCGGCATC
GGCATCGGCGGCGCGCTGGCCTCCAAACTTGCCATGACGGTCGCGCCGCGCTGGCTGCTGTTCGGCGCCGCGCTGGTGCT
GGCCGTCGGCCTGCTGTTCGGTTCCACCCTCGACGGCGAGGTGTCCTACCTGCCGACGCTGCTGCCGCTGCTGATCGTGA
TCGGCTTCGGCGTCGGTGTCGCGATGGTCGTCACCCCGCTGTGCGTGCTCGTCGGCGTACCGCCCTCCGACATCGGCCCG
CTCTCGGCGGTCGGCCAGATGTTCATGAACCTCGGCACCCCGATGGCGATCGGCATCCTCACCCCGGTCGCGGTCTCGCG
CACGCTGTCGCTGGGCGGCACCACCGGCAAGGTCTCGGCGATGACCGACGCCCAGATCGTCGCGCTCGGCGAGGGCTACA
CACTGGTGCTGGCGGTGTGCGCGGGCGTGGCCGCCGTCATCGGCCTGATCGCGCTCACCCTGCGCTTCACCCCCGAACAG
ATCGCCAGGGCCCAGCACGCCCAGGAGGAAGCGCAGCGGAGCTGA

Upstream 100 bases:

>100_bases
TTCGCGCTCGCGGACATCGGCGCGCTCACCGGTTCGGCAGGTCAGTAGCCGTACCCGTGCGTACCGGAAACCGTCGAGCC
GTCCACTACAGTGGCTCACT

Downstream 100 bases:

>100_bases
CCCGGCTCCGGCCGCTGACCCGGCTCAGAGGCGGTGTCCGACGTGCTCTGAGCCGGGGCGGCCTCCGTGGTGACGCTCCG
CTGCCGGCTCCGGCCGCTGA

Product: putative transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Efflux protein A [H]

Number of amino acids: Translated: 494; Mature: 494

Protein sequence:

>494_residues
MLENPAATTQFPVTQRAFGLAILVLSGLQLMVVLDGTVVIFALPRLQDQMGLSSAGSAWIVTAYGLTFAGLMLLGGRLGD
AFGRKRMLIAGVGLFTVASLLCGLAHWQAMLIAARALQGAGAAIAAPVAFALVATTFAPGKARNQAIAIVGSMVGIGSVG
GLVVGGALTQLSWRWIFLINVPIGALIILGAIYCLADTGHHRVALDARGAVLGTLACAAIVFGATEGPELGWSHPAVIGA
LIGGAILLVVFVIAERNVDDPLLPWSLFDSRDRVTTFVLILLAGGVLGAMTYFVAQFLQNVLGYGPLQAGVASIPFTVGI
GIGGALASKLAMTVAPRWLLFGAALVLAVGLLFGSTLDGEVSYLPTLLPLLIVIGFGVGVAMVVTPLCVLVGVPPSDIGP
LSAVGQMFMNLGTPMAIGILTPVAVSRTLSLGGTTGKVSAMTDAQIVALGEGYTLVLAVCAGVAAVIGLIALTLRFTPEQ
IARAQHAQEEAQRS

Sequences:

>Translated_494_residues
MLENPAATTQFPVTQRAFGLAILVLSGLQLMVVLDGTVVIFALPRLQDQMGLSSAGSAWIVTAYGLTFAGLMLLGGRLGD
AFGRKRMLIAGVGLFTVASLLCGLAHWQAMLIAARALQGAGAAIAAPVAFALVATTFAPGKARNQAIAIVGSMVGIGSVG
GLVVGGALTQLSWRWIFLINVPIGALIILGAIYCLADTGHHRVALDARGAVLGTLACAAIVFGATEGPELGWSHPAVIGA
LIGGAILLVVFVIAERNVDDPLLPWSLFDSRDRVTTFVLILLAGGVLGAMTYFVAQFLQNVLGYGPLQAGVASIPFTVGI
GIGGALASKLAMTVAPRWLLFGAALVLAVGLLFGSTLDGEVSYLPTLLPLLIVIGFGVGVAMVVTPLCVLVGVPPSDIGP
LSAVGQMFMNLGTPMAIGILTPVAVSRTLSLGGTTGKVSAMTDAQIVALGEGYTLVLAVCAGVAAVIGLIALTLRFTPEQ
IARAQHAQEEAQRS
>Mature_494_residues
MLENPAATTQFPVTQRAFGLAILVLSGLQLMVVLDGTVVIFALPRLQDQMGLSSAGSAWIVTAYGLTFAGLMLLGGRLGD
AFGRKRMLIAGVGLFTVASLLCGLAHWQAMLIAARALQGAGAAIAAPVAFALVATTFAPGKARNQAIAIVGSMVGIGSVG
GLVVGGALTQLSWRWIFLINVPIGALIILGAIYCLADTGHHRVALDARGAVLGTLACAAIVFGATEGPELGWSHPAVIGA
LIGGAILLVVFVIAERNVDDPLLPWSLFDSRDRVTTFVLILLAGGVLGAMTYFVAQFLQNVLGYGPLQAGVASIPFTVGI
GIGGALASKLAMTVAPRWLLFGAALVLAVGLLFGSTLDGEVSYLPTLLPLLIVIGFGVGVAMVVTPLCVLVGVPPSDIGP
LSAVGQMFMNLGTPMAIGILTPVAVSRTLSLGGTTGKVSAMTDAQIVALGEGYTLVLAVCAGVAAVIGLIALTLRFTPEQ
IARAQHAQEEAQRS

Specific function: Translocase That Confers Resistance To Substances Of High Hydrophobicity. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=429, Percent_Identity=28.2051282051282, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI1788710, Length=419, Percent_Identity=27.2076372315036, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI87081983, Length=331, Percent_Identity=28.3987915407855, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1790195, Length=330, Percent_Identity=29.0909090909091, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI1788392, Length=298, Percent_Identity=29.8657718120805, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1790146, Length=183, Percent_Identity=27.3224043715847, Blast_Score=63, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6323520, Length=385, Percent_Identity=27.5324675324675, Blast_Score=127, Evalue=6e-30,
Organism=Saccharomyces cerevisiae, GI6324954, Length=389, Percent_Identity=26.2210796915167, Blast_Score=113, Evalue=6e-26,
Organism=Saccharomyces cerevisiae, GI6323935, Length=424, Percent_Identity=24.2924528301887, Blast_Score=101, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6319770, Length=387, Percent_Identity=27.6485788113695, Blast_Score=92, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6322958, Length=475, Percent_Identity=24.6315789473684, Blast_Score=83, Evalue=8e-17,
Organism=Saccharomyces cerevisiae, GI6325455, Length=152, Percent_Identity=34.2105263157895, Blast_Score=76, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6321663, Length=153, Percent_Identity=31.3725490196078, Blast_Score=70, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 50418; Mature: 50418

Theoretical pI: Translated: 7.89; Mature: 7.89

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLENPAATTQFPVTQRAFGLAILVLSGLQLMVVLDGTVVIFALPRLQDQMGLSSAGSAWI
CCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEECCCEEEEEEHHHHHHCCCCCCCCEEE
VTAYGLTFAGLMLLGGRLGDAFGRKRMLIAGVGLFTVASLLCGLAHWQAMLIAARALQGA
EEHHHHHHHHHHHHCCHHHHHHCCCCHHEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GAAIAAPVAFALVATTFAPGKARNQAIAIVGSMVGIGSVGGLVVGGALTQLSWRWIFLIN
CCHHHHHHHHHHHHHHCCCCCCCCCCEEEHHHHHCCCCCCHHHHHHHHHHCCEEEEEEEE
VPIGALIILGAIYCLADTGHHRVALDARGAVLGTLACAAIVFGATEGPELGWSHPAVIGA
CCHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHH
LIGGAILLVVFVIAERNVDDPLLPWSLFDSRDRVTTFVLILLAGGVLGAMTYFVAQFLQN
HHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VLGYGPLQAGVASIPFTVGIGIGGALASKLAMTVAPRWLLFGAALVLAVGLLFGSTLDGE
HHCCCCHHHHHHHCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
VSYLPTLLPLLIVIGFGVGVAMVVTPLCVLVGVPPSDIGPLSAVGQMFMNLGTPMAIGIL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCHHHHHH
TPVAVSRTLSLGGTTGKVSAMTDAQIVALGEGYTLVLAVCAGVAAVIGLIALTLRFTPEQ
HHHHHHHHHCCCCCCCCEEEECCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCHHH
IARAQHAQEEAQRS
HHHHHHHHHHHHCC
>Mature Secondary Structure
MLENPAATTQFPVTQRAFGLAILVLSGLQLMVVLDGTVVIFALPRLQDQMGLSSAGSAWI
CCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEECCCEEEEEEHHHHHHCCCCCCCCEEE
VTAYGLTFAGLMLLGGRLGDAFGRKRMLIAGVGLFTVASLLCGLAHWQAMLIAARALQGA
EEHHHHHHHHHHHHCCHHHHHHCCCCHHEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GAAIAAPVAFALVATTFAPGKARNQAIAIVGSMVGIGSVGGLVVGGALTQLSWRWIFLIN
CCHHHHHHHHHHHHHHCCCCCCCCCCEEEHHHHHCCCCCCHHHHHHHHHHCCEEEEEEEE
VPIGALIILGAIYCLADTGHHRVALDARGAVLGTLACAAIVFGATEGPELGWSHPAVIGA
CCHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHH
LIGGAILLVVFVIAERNVDDPLLPWSLFDSRDRVTTFVLILLAGGVLGAMTYFVAQFLQN
HHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VLGYGPLQAGVASIPFTVGIGIGGALASKLAMTVAPRWLLFGAALVLAVGLLFGSTLDGE
HHCCCCHHHHHHHCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
VSYLPTLLPLLIVIGFGVGVAMVVTPLCVLVGVPPSDIGPLSAVGQMFMNLGTPMAIGIL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCHHHHHH
TPVAVSRTLSLGGTTGKVSAMTDAQIVALGEGYTLVLAVCAGVAAVIGLIALTLRFTPEQ
HHHHHHHHHCCCCCCCCEEEECCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCHHH
IARAQHAQEEAQRS
HHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]