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The map label for this gene is proS

Identifier: 54026044

GI number: 54026044

Start: 4222401

End: 4224158

Strand: Reverse

Name: proS

Synonym: nfa40730

Alternate gene names: 54026044

Gene position: 4224158-4222401 (Counterclockwise)

Preceding gene: 54026047

Following gene: 54026043

Centisome position: 70.15

GC content: 70.48

Gene sequence:

>1758_bases
GTGATCACCCGCCTCTCCCACCTGTTCCTGCGTACCCTGCGCGACGACCCCGCCGACGCCGAGGTGCCCAGTCACAAACT
GCTGGTCCGCGCCGGGTACGTGCGCCGTGTCGCCCCGGGTGTGTATTCGTGGTTGCCGCTGGGGCTACGGGTGCTGCGCA
AGGTCGAGGACGTCGTGCGGGCGGAGATGAACGCCATCGGCGGCCAGGAGATCTCGCTGCCCGCGCTGCTGCCGCGCGAC
CCCTACGAGACCACCAACCGCTGGACCGAATACGGCGACAGCCTGTTCCGGCTGCACGATCGCAAGGGCGCGGACTACCT
GCTCGGCCCCACCCACGAGGAGCTGTTCGCGCTCACCGTGAAGGGTGAGTACAACTCCTACAAGGATCTGCCGGTCACGC
TGTACCAGATCCAGACCAAGTACCGCGACGAGGAGCGGCCGCGCGCGGGGATCCTGCGCGGGCGCGAGTTCGTCATGAAG
GACTCCTACTCCTTCGATCTGGACGAGGACGGGCTGAAGGCGAGCTACAACGCGCACCGCGAGGCCTACCAGCGCATCTT
CGACCGGCTCGGCGTCAAGTACGTGATCGTGGCGGCCACCTCCGGCGCGATGGGCGGCAGCGCCTCCGAGGAGTTCCTGG
CCGACAGCCCGGTCGGCGAGGACACCTACGTCATCTGCCGCGAGTCCGGCTACGCGGCGAATGTGGAGGCGGTGGTCACG
CCCGCGCCCGAACCGCAGCCGATCGAGGGCAGGCCCGCCGCGGTCGTGCACGACACCCCCGACACCCCCACCATCGCGTC
GCTGGTCGAGTGGGCCAACGCCGCGGGCATCGCCGAGCAGTTCGGGCGTCCGGTCACCGCCGCCGACACGCTCAAGAACG
TGATGGTCAAGCTGCGCCACCCGGACGGCAAGACCGAGATCGTCGGCATCGGCGTGCCCGGCGACCGCGAGGTGGACGAC
AAGCGGCTCGGCGCTTCCGTCGAGCCCGCCGAGGTGGAACTGCTCACCGACGCCGACTTCGCCGCCAATCCCTTCCTGGT
GAAGGGCTACATCGGCCCGAAGGCGTTGCAGGAGAACGGCATCCGCTACCTCGTCGATCCGCGGGTCGGCACCGGCACCG
CCTGGATCACCGGCGCGGACAGCCCGGGCAAGCACGTGGTCGGCTTGATCGCGGGCCGCGACTTCACCCCCGACGGCACC
ATCGAGGCCGCCGAGGTCCGCGACGGCGACCCGTCGCCGGACGGGCGCGGCACCCTGGAGGCGGCGCGCGGCATCGAGAT
CGGCCACATCTTCCAGCTCGGCTACAAGTACACCGACGCGTTCGAGGTCGACGTGCTCGGCGAGAACGGCAAGCCGGTGC
GGCTCATCATGGGCTCCTACGGCATCGGCGTCTCCCGCATGGTCGCGGTGATCGCCGAGCAGCAGCACGACGAGAAGGGC
CTGCGCTGGCCCGCCGCGGTCGCACCGTTCGACGTGCACGTGGTCGTCGCGAACAAGGACGAGGCCGCCCGCACCGGCGC
CGAGCAGGTGGTGGCCGGACTGGACGCGCAGGGGCTGGACGTGCTGTTCGACGACCGCACCGCCTCGCCCGGGGTGAAGT
TCAAGGACGCCGAACTGCTCGGCATGCCGTGGGTGGTGGTGATCGGCCGTGGCTGGGCCGACGGCACGGTGGAGTTGCGC
AACCGCTTCACCGGCGAGGCCGAGAACGTGCCCGCCGCGGAGGCGGTGGCGACGGTGGTCGCGCGGATCCGCGGCTGA

Upstream 100 bases:

>100_bases
ACCGTCGGACTTGGTTTCGGAAGGAGGCAGCAGCACCAGCACGAGCGGATACGGTAATGCCTTTGAGCGGCGATTCGAGC
GGGGACTACGCTGTGGAGCC

Downstream 100 bases:

>100_bases
ATCCTGGCGCTGCCCCGGCCCGTCTCGGGCCGGGGCAGCGCCGTTCCCAGGCCGGGCGAACCGGCCGTGAGAGAGTTCGG
CTCCACCTACGCCACCGTAA

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS

Number of amino acids: Translated: 585; Mature: 585

Protein sequence:

>585_residues
MITRLSHLFLRTLRDDPADAEVPSHKLLVRAGYVRRVAPGVYSWLPLGLRVLRKVEDVVRAEMNAIGGQEISLPALLPRD
PYETTNRWTEYGDSLFRLHDRKGADYLLGPTHEELFALTVKGEYNSYKDLPVTLYQIQTKYRDEERPRAGILRGREFVMK
DSYSFDLDEDGLKASYNAHREAYQRIFDRLGVKYVIVAATSGAMGGSASEEFLADSPVGEDTYVICRESGYAANVEAVVT
PAPEPQPIEGRPAAVVHDTPDTPTIASLVEWANAAGIAEQFGRPVTAADTLKNVMVKLRHPDGKTEIVGIGVPGDREVDD
KRLGASVEPAEVELLTDADFAANPFLVKGYIGPKALQENGIRYLVDPRVGTGTAWITGADSPGKHVVGLIAGRDFTPDGT
IEAAEVRDGDPSPDGRGTLEAARGIEIGHIFQLGYKYTDAFEVDVLGENGKPVRLIMGSYGIGVSRMVAVIAEQQHDEKG
LRWPAAVAPFDVHVVVANKDEAARTGAEQVVAGLDAQGLDVLFDDRTASPGVKFKDAELLGMPWVVVIGRGWADGTVELR
NRFTGEAENVPAAEAVATVVARIRG

Sequences:

>Translated_585_residues
MITRLSHLFLRTLRDDPADAEVPSHKLLVRAGYVRRVAPGVYSWLPLGLRVLRKVEDVVRAEMNAIGGQEISLPALLPRD
PYETTNRWTEYGDSLFRLHDRKGADYLLGPTHEELFALTVKGEYNSYKDLPVTLYQIQTKYRDEERPRAGILRGREFVMK
DSYSFDLDEDGLKASYNAHREAYQRIFDRLGVKYVIVAATSGAMGGSASEEFLADSPVGEDTYVICRESGYAANVEAVVT
PAPEPQPIEGRPAAVVHDTPDTPTIASLVEWANAAGIAEQFGRPVTAADTLKNVMVKLRHPDGKTEIVGIGVPGDREVDD
KRLGASVEPAEVELLTDADFAANPFLVKGYIGPKALQENGIRYLVDPRVGTGTAWITGADSPGKHVVGLIAGRDFTPDGT
IEAAEVRDGDPSPDGRGTLEAARGIEIGHIFQLGYKYTDAFEVDVLGENGKPVRLIMGSYGIGVSRMVAVIAEQQHDEKG
LRWPAAVAPFDVHVVVANKDEAARTGAEQVVAGLDAQGLDVLFDDRTASPGVKFKDAELLGMPWVVVIGRGWADGTVELR
NRFTGEAENVPAAEAVATVVARIRG
>Mature_585_residues
MITRLSHLFLRTLRDDPADAEVPSHKLLVRAGYVRRVAPGVYSWLPLGLRVLRKVEDVVRAEMNAIGGQEISLPALLPRD
PYETTNRWTEYGDSLFRLHDRKGADYLLGPTHEELFALTVKGEYNSYKDLPVTLYQIQTKYRDEERPRAGILRGREFVMK
DSYSFDLDEDGLKASYNAHREAYQRIFDRLGVKYVIVAATSGAMGGSASEEFLADSPVGEDTYVICRESGYAANVEAVVT
PAPEPQPIEGRPAAVVHDTPDTPTIASLVEWANAAGIAEQFGRPVTAADTLKNVMVKLRHPDGKTEIVGIGVPGDREVDD
KRLGASVEPAEVELLTDADFAANPFLVKGYIGPKALQENGIRYLVDPRVGTGTAWITGADSPGKHVVGLIAGRDFTPDGT
IEAAEVRDGDPSPDGRGTLEAARGIEIGHIFQLGYKYTDAFEVDVLGENGKPVRLIMGSYGIGVSRMVAVIAEQQHDEKG
LRWPAAVAPFDVHVVVANKDEAARTGAEQVVAGLDAQGLDVLFDDRTASPGVKFKDAELLGMPWVVVIGRGWADGTVELR
NRFTGEAENVPAAEAVATVVARIRG

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids su

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily

Homologues:

Organism=Homo sapiens, GI34303926, Length=228, Percent_Identity=42.1052631578947, Blast_Score=202, Evalue=1e-51,
Organism=Escherichia coli, GI1786392, Length=592, Percent_Identity=42.0608108108108, Blast_Score=434, Evalue=1e-122,
Organism=Caenorhabditis elegans, GI115532348, Length=210, Percent_Identity=35.7142857142857, Blast_Score=136, Evalue=3e-32,
Organism=Saccharomyces cerevisiae, GI6320931, Length=231, Percent_Identity=41.991341991342, Blast_Score=183, Evalue=6e-47,
Organism=Drosophila melanogaster, GI24656200, Length=223, Percent_Identity=40.3587443946188, Blast_Score=174, Evalue=2e-43,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): SYP_NOCFA (Q5YSB9)

Other databases:

- EMBL:   AP006618
- RefSeq:   YP_120286.1
- ProteinModelPortal:   Q5YSB9
- SMR:   Q5YSB9
- GeneID:   3111394
- GenomeReviews:   AP006618_GR
- KEGG:   nfa:nfa40730
- NMPDR:   fig|247156.1.peg.3932
- HOGENOM:   HBG403504
- OMA:   DFVLGPT
- ProtClustDB:   PRK09194
- BioCyc:   NFAR247156:NFA40730-MONOMER
- BRENDA:   6.1.1.15
- GO:   GO:0005737
- HAMAP:   MF_01569
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004500
- InterPro:   IPR007214
- Gene3D:   G3DSA:3.40.50.800
- PRINTS:   PR01046
- TIGRFAMs:   TIGR00409

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; PF04073 YbaK; SSF52954 Anticodon_bd; SSF55826 YbaK/aa-tRNA-synth-assoc-reg

EC number: =6.1.1.15

Molecular weight: Translated: 63550; Mature: 63550

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITRLSHLFLRTLRDDPADAEVPSHKLLVRAGYVRRVAPGVYSWLPLGLRVLRKVEDVVR
CCCHHHHHHHHHHCCCCCCCCCCCCEEEEEECHHHHHCCHHHHHHHHHHHHHHHHHHHHH
AEMNAIGGQEISLPALLPRDPYETTNRWTEYGDSLFRLHDRKGADYLLGPTHEELFALTV
HHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHCCHHEEECCCCCCCEEECCCCCCEEEEEE
KGEYNSYKDLPVTLYQIQTKYRDEERPRAGILRGREFVMKDSYSFDLDEDGLKASYNAHR
ECCCCCCCCCCEEEEEEEHHHCCCCCCCHHHHCCCEEEEECCCCCCCCCCCCCCCHHHHH
EAYQRIFDRLGVKYVIVAATSGAMGGSASEEFLADSPVGEDTYVICRESGYAANVEAVVT
HHHHHHHHHCCCEEEEEEEECCCCCCCCCHHHHCCCCCCCCCEEEEECCCCEECCEEEEE
PAPEPQPIEGRPAAVVHDTPDTPTIASLVEWANAAGIAEQFGRPVTAADTLKNVMVKLRH
CCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHCCHHHHHCCCCCHHHHHHHHHEEEEC
PDGKTEIVGIGVPGDREVDDKRLGASVEPAEVELLTDADFAANPFLVKGYIGPKALQENG
CCCCEEEEEEECCCCCCCCHHHCCCCCCCCEEEEEECCCCCCCCEEEEECCCHHHHHHCC
IRYLVDPRVGTGTAWITGADSPGKHVVGLIAGRDFTPDGTIEAAEVRDGDPSPDGRGTLE
CEEEECCCCCCCEEEEECCCCCCCEEEEEEECCCCCCCCCEEEEECCCCCCCCCCCCHHH
AARGIEIGHIFQLGYKYTDAFEVDVLGENGKPVRLIMGSYGIGVSRMVAVIAEQQHDEKG
HHCCCEEEEEEEECCEECCEEEEEEECCCCCEEEEEEECCCCCHHHHHHHHHHHCCCCCC
LRWPAAVAPFDVHVVVANKDEAARTGAEQVVAGLDAQGLDVLFDDRTASPGVKFKDAELL
CCCCCEECCEEEEEEEECCCHHHHCCHHHHHHCCCCCCCEEEEECCCCCCCCEECCCEEE
GMPWVVVIGRGWADGTVELRNRFTGEAENVPAAEAVATVVARIRG
CCCEEEEEECCCCCCCHHHHHCCCCCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MITRLSHLFLRTLRDDPADAEVPSHKLLVRAGYVRRVAPGVYSWLPLGLRVLRKVEDVVR
CCCHHHHHHHHHHCCCCCCCCCCCCEEEEEECHHHHHCCHHHHHHHHHHHHHHHHHHHHH
AEMNAIGGQEISLPALLPRDPYETTNRWTEYGDSLFRLHDRKGADYLLGPTHEELFALTV
HHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHCCHHEEECCCCCCCEEECCCCCCEEEEEE
KGEYNSYKDLPVTLYQIQTKYRDEERPRAGILRGREFVMKDSYSFDLDEDGLKASYNAHR
ECCCCCCCCCCEEEEEEEHHHCCCCCCCHHHHCCCEEEEECCCCCCCCCCCCCCCHHHHH
EAYQRIFDRLGVKYVIVAATSGAMGGSASEEFLADSPVGEDTYVICRESGYAANVEAVVT
HHHHHHHHHCCCEEEEEEEECCCCCCCCCHHHHCCCCCCCCCEEEEECCCCEECCEEEEE
PAPEPQPIEGRPAAVVHDTPDTPTIASLVEWANAAGIAEQFGRPVTAADTLKNVMVKLRH
CCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHCCHHHHHCCCCCHHHHHHHHHEEEEC
PDGKTEIVGIGVPGDREVDDKRLGASVEPAEVELLTDADFAANPFLVKGYIGPKALQENG
CCCCEEEEEEECCCCCCCCHHHCCCCCCCCEEEEEECCCCCCCCEEEEECCCHHHHHHCC
IRYLVDPRVGTGTAWITGADSPGKHVVGLIAGRDFTPDGTIEAAEVRDGDPSPDGRGTLE
CEEEECCCCCCCEEEEECCCCCCCEEEEEEECCCCCCCCCEEEEECCCCCCCCCCCCHHH
AARGIEIGHIFQLGYKYTDAFEVDVLGENGKPVRLIMGSYGIGVSRMVAVIAEQQHDEKG
HHCCCEEEEEEEECCEECCEEEEEEECCCCCEEEEEEECCCCCHHHHHHHHHHHCCCCCC
LRWPAAVAPFDVHVVVANKDEAARTGAEQVVAGLDAQGLDVLFDDRTASPGVKFKDAELL
CCCCCEECCEEEEEEEECCCHHHHCCHHHHHHCCCCCCCEEEEECCCCCCCCEECCCEEE
GMPWVVVIGRGWADGTVELRNRFTGEAENVPAAEAVATVVARIRG
CCCEEEEEECCCCCCCHHHHHCCCCCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA