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The map label for this gene is aph [H]

Identifier: 54025818

GI number: 54025818

Start: 4066002

End: 4066793

Strand: Reverse

Name: aph [H]

Synonym: nfa38480

Alternate gene names: 54025818

Gene position: 4066793-4066002 (Counterclockwise)

Preceding gene: 54025819

Following gene: 54025816

Centisome position: 67.54

GC content: 73.61

Gene sequence:

>792_bases
GTGCGGCTGGCCGAGGGCGATCCGGAGCTGATCGAATCGACCCGCACCGCCCTGGACACCCTCGACGACCTGCGCGAGCC
GCTGCCGGAGCCGGTGGCGGCGGTTCTCGGGCCGCGCCCGCGGTGGTCGGACGAGCACGAGGGCCGCAGCGGCGGCGTGG
TCCGCGCGGGCGGGCACTGGGTCAAACGCGGCCCGCGCGCGGTGGCGGAGTACGAACGACTGGAATGGTTGCGGCACAAC
GGTATCCGCGTGCCCGAGGTGGTGGTGTGCGCCGGTGGCGTGCTGGTGCTCGCCGACGCGGGGGTGCCCAGTCTGGCCAC
CCGCGCCGACACGGAGGGCGTCCCGATCGGCGCGACGATGGGCGCGCTGCTGCGGCGCCTGCACGCGTTGCCCGTCGCCG
GCTGCCCGTTCGACGGCCGCCTGGACGGTGTGCTGGCCCAGGCGCGCCGCAACGTGATCGAAGGGCTGGTCGACGCCGAC
GACTTCGACGACGACAACGCCGACCACACGCCCGAATCGGTGCTGGCCGAGCTGCTGGATCGGCGGCCGCCCGAGGCGGA
TCTGGTGGTCGCCCACGGCGACTACACCCCGCCCAACGTGCTCGACGGCGACCTGCTGATCGACGTCGGCGCACTCGGGG
TCGCCGATCGCTACCGCGACCTGGCGCTGGCCGAACGTGACCTGCGCGAGTACGGACCGGCGGAGGTGACGGCCTTCTTC
GACGCCTACGGCCTGCCCGAGCCGGATCCCGCGCGGCTGGAGTACTACCGCCTGCTCGACGAACTGTTCTGA

Upstream 100 bases:

>100_bases
TCCGGCGGCGTTCTCCTTCGCCGCCCATCATCTCGGACTGACCTTGGCCGCACGCGGCGAGCGGGATGCGGCGCGGGAGG
CGCTCACCGAGGCGCTGCGG

Downstream 100 bases:

>100_bases
GTCGGGACTGCTCGGCGGTCATGGCTTCTCGGCGGTCAGGATTTCTTGGCGTAGTGCCGGGCGGCCTTGGCGCGATTGCC
GCAGGTGGCCATCGAATGCC

Product: putative aminoglycoside 3'-O-phosphotransferase

Products: NA

Alternate protein names: APH(3')V; Kanamycin kinase, type V; Neomycin-kanamycin phosphotransferase type V [H]

Number of amino acids: Translated: 263; Mature: 263

Protein sequence:

>263_residues
MRLAEGDPELIESTRTALDTLDDLREPLPEPVAAVLGPRPRWSDEHEGRSGGVVRAGGHWVKRGPRAVAEYERLEWLRHN
GIRVPEVVVCAGGVLVLADAGVPSLATRADTEGVPIGATMGALLRRLHALPVAGCPFDGRLDGVLAQARRNVIEGLVDAD
DFDDDNADHTPESVLAELLDRRPPEADLVVAHGDYTPPNVLDGDLLIDVGALGVADRYRDLALAERDLREYGPAEVTAFF
DAYGLPEPDPARLEYYRLLDELF

Sequences:

>Translated_263_residues
MRLAEGDPELIESTRTALDTLDDLREPLPEPVAAVLGPRPRWSDEHEGRSGGVVRAGGHWVKRGPRAVAEYERLEWLRHN
GIRVPEVVVCAGGVLVLADAGVPSLATRADTEGVPIGATMGALLRRLHALPVAGCPFDGRLDGVLAQARRNVIEGLVDAD
DFDDDNADHTPESVLAELLDRRPPEADLVVAHGDYTPPNVLDGDLLIDVGALGVADRYRDLALAERDLREYGPAEVTAFF
DAYGLPEPDPARLEYYRLLDELF
>Mature_263_residues
MRLAEGDPELIESTRTALDTLDDLREPLPEPVAAVLGPRPRWSDEHEGRSGGVVRAGGHWVKRGPRAVAEYERLEWLRHN
GIRVPEVVVCAGGVLVLADAGVPSLATRADTEGVPIGATMGALLRRLHALPVAGCPFDGRLDGVLAQARRNVIEGLVDAD
DFDDDNADHTPESVLAELLDRRPPEADLVVAHGDYTPPNVLDGDLLIDVGALGVADRYRDLALAERDLREYGPAEVTAFF
DAYGLPEPDPARLEYYRLLDELF

Specific function: Resistance to kanamycin and structurally-related aminoglycosides, including amikacin [H]

COG id: COG3231

COG function: function code J; Aminoglycoside phosphotransferase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aminoglycoside phosphotransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002575
- InterPro:   IPR011009 [H]

Pfam domain/function: PF01636 APH [H]

EC number: =2.7.1.95 [H]

Molecular weight: Translated: 28602; Mature: 28602

Theoretical pI: Translated: 4.29; Mature: 4.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLAEGDPELIESTRTALDTLDDLREPLPEPVAAVLGPRPRWSDEHEGRSGGVVRAGGHW
CCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCCCCCEEECCCHH
VKRGPRAVAEYERLEWLRHNGIRVPEVVVCAGGVLVLADAGVPSLATRADTEGVPIGATM
HHCCCHHHHHHHHHHHHHHCCCCCCHHEEECCCEEEEECCCCCHHHHHCCCCCCCCHHHH
GALLRRLHALPVAGCPFDGRLDGVLAQARRNVIEGLVDADDFDDDNADHTPESVLAELLD
HHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHH
RRPPEADLVVAHGDYTPPNVLDGDLLIDVGALGVADRYRDLALAERDLREYGPAEVTAFF
CCCCCCCEEEEECCCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
DAYGLPEPDPARLEYYRLLDELF
HHCCCCCCCHHHHHHHHHHHHHC
>Mature Secondary Structure
MRLAEGDPELIESTRTALDTLDDLREPLPEPVAAVLGPRPRWSDEHEGRSGGVVRAGGHW
CCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCCCCCEEECCCHH
VKRGPRAVAEYERLEWLRHNGIRVPEVVVCAGGVLVLADAGVPSLATRADTEGVPIGATM
HHCCCHHHHHHHHHHHHHHCCCCCCHHEEECCCEEEEECCCCCHHHHHCCCCCCCCHHHH
GALLRRLHALPVAGCPFDGRLDGVLAQARRNVIEGLVDADDFDDDNADHTPESVLAELLD
HHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHH
RRPPEADLVVAHGDYTPPNVLDGDLLIDVGALGVADRYRDLALAERDLREYGPAEVTAFF
CCCCCCCEEEEECCCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
DAYGLPEPDPARLEYYRLLDELF
HHCCCCCCCHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6310563; 2987648 [H]