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The map label for this gene is hmp [H]

Identifier: 54025655

GI number: 54025655

Start: 3902214

End: 3903449

Strand: Reverse

Name: hmp [H]

Synonym: nfa36850

Alternate gene names: 54025655

Gene position: 3903449-3902214 (Counterclockwise)

Preceding gene: 54025656

Following gene: 54025653

Centisome position: 64.83

GC content: 71.6

Gene sequence:

>1236_bases
ATGACGATCGTTTCGCCGTCGGCGCTCGGCGCCCCCGCGGAATTGGAAGCCCACCATGCCGAGGTGGTTCGCGCCACCCT
CCCGGTCATCGCCGCGCACATCGACGACATCACCGCGCTGTTCTACCGGAAGCTGTTCACCGCGCACCCCGAGCTGCTCG
CGAATCTGTTCAACCGTGGCAACCAGGCGCAGGGCGCACAGCAGCGGGCACTGGCCGCCTCGATCGCCACCTTCGCCGCC
CACCTGGTGGACCCGGCGCTGCCGCATCCGGCCGACCTGCTCGCCCGGATCGGCCACAAGCACGCCTCCCTCGGCGTCGT
CGCCGAGCAGTACCCGATCGTGCACGAGCACCTGTTCGCCGCCATCGTGGCGGTGCTCGACGCCGACACGGTGACACCCG
AGGTCGCCGCCGCCTGGGATCGGGTCTACTGGCTGATGGCCGACGCGCTGATCGGTTTCGAGGGCGAGCTCTACCGGGCC
GCGGGCGTGGCACCCGGTGACGTGTTCCGCGAGACCGTCGTGGTCGACCGTGTCGAGGATCCCTCGGGTGTCGTCACCTT
CGTCGTCGAGTCCGCCGACCCGGCCGCCCCGCTGCCGGATTTCCTTCCCGGTCAGTATGTCTCGGTCGGCGCGCGGCTAC
TCGACGGTGCCCGCCAGCTGCGCCAGTACAGCCTGGTCGCCGCACCCGGCGGCGGCCGCCTCGCCTTCGCCGTCCGCCGT
GTCGAGCCGACCCCGGAACAGCCCGCCGGCGAGGTCTCCACCTGGCTGCACACCCAGGTCCGTCCCGGCGACACCCTCGA
GATCACCCTCCCGTTCGGCGACCTGGTCGTCGACACCGACGCCGCCACCCCGCTGGTGCTGATCTCGGCGGGCATCGGCA
TCACCCCCATGATCGGCATCCTCGAACACCTCGCCGCCGCGACCCCGAACCGCCGCGTCCTCGCGGTCCACGCCGACCGC
ACCCCCGACACCCACCCGCTGCGCGCCGTCCAGGCCGACCTGATCGCCGCCCTCCCCGCGGCCACCCTCGAACTCTGGTA
CGAACACCCGACCCCGGGCGCGCACGCGGGCATGCCGACCCTCACCGACCTCACTCTGCCCCCCGACGCCGACATCTACC
TCTGCGGCGGACCCGCCTTCCTCCAGTCCGCCCGGGCCCAACTCGCCGCCATCGGAATCCCCACCGCCCGTATCCATTTC
GAGCTGTTCACCCCGAACGACTGGCTCCTGGACTGA

Upstream 100 bases:

>100_bases
TTACTTCACCGCCGCTCGCCTCGCTTTTTCCGAGCGGAATTGGTTACCGTCATTTCCGTGGCGTTCGTTGCCACGATGGA
ATCTCGACGAGGAGAACCCC

Downstream 100 bases:

>100_bases
GCCCGGAAGACGCCGCGGGGCGGGTGATGTCCGGGCCGGGCACGCCGTCAGGGTGGCGGCCGGCGCGGTCAGGCGGCGGA
GGTGACGCGGTAGACGTCGT

Product: putative flavohemoprotein

Products: NA

Alternate protein names: Flavohemoglobin; Hemoglobin-like protein; Nitric oxide dioxygenase; NO oxygenase; NOD [H]

Number of amino acids: Translated: 411; Mature: 410

Protein sequence:

>411_residues
MTIVSPSALGAPAELEAHHAEVVRATLPVIAAHIDDITALFYRKLFTAHPELLANLFNRGNQAQGAQQRALAASIATFAA
HLVDPALPHPADLLARIGHKHASLGVVAEQYPIVHEHLFAAIVAVLDADTVTPEVAAAWDRVYWLMADALIGFEGELYRA
AGVAPGDVFRETVVVDRVEDPSGVVTFVVESADPAAPLPDFLPGQYVSVGARLLDGARQLRQYSLVAAPGGGRLAFAVRR
VEPTPEQPAGEVSTWLHTQVRPGDTLEITLPFGDLVVDTDAATPLVLISAGIGITPMIGILEHLAAATPNRRVLAVHADR
TPDTHPLRAVQADLIAALPAATLELWYEHPTPGAHAGMPTLTDLTLPPDADIYLCGGPAFLQSARAQLAAIGIPTARIHF
ELFTPNDWLLD

Sequences:

>Translated_411_residues
MTIVSPSALGAPAELEAHHAEVVRATLPVIAAHIDDITALFYRKLFTAHPELLANLFNRGNQAQGAQQRALAASIATFAA
HLVDPALPHPADLLARIGHKHASLGVVAEQYPIVHEHLFAAIVAVLDADTVTPEVAAAWDRVYWLMADALIGFEGELYRA
AGVAPGDVFRETVVVDRVEDPSGVVTFVVESADPAAPLPDFLPGQYVSVGARLLDGARQLRQYSLVAAPGGGRLAFAVRR
VEPTPEQPAGEVSTWLHTQVRPGDTLEITLPFGDLVVDTDAATPLVLISAGIGITPMIGILEHLAAATPNRRVLAVHADR
TPDTHPLRAVQADLIAALPAATLELWYEHPTPGAHAGMPTLTDLTLPPDADIYLCGGPAFLQSARAQLAAIGIPTARIHF
ELFTPNDWLLD
>Mature_410_residues
TIVSPSALGAPAELEAHHAEVVRATLPVIAAHIDDITALFYRKLFTAHPELLANLFNRGNQAQGAQQRALAASIATFAAH
LVDPALPHPADLLARIGHKHASLGVVAEQYPIVHEHLFAAIVAVLDADTVTPEVAAAWDRVYWLMADALIGFEGELYRAA
GVAPGDVFRETVVVDRVEDPSGVVTFVVESADPAAPLPDFLPGQYVSVGARLLDGARQLRQYSLVAAPGGGRLAFAVRRV
EPTPEQPAGEVSTWLHTQVRPGDTLEITLPFGDLVVDTDAATPLVLISAGIGITPMIGILEHLAAATPNRRVLAVHADRT
PDTHPLRAVQADLIAALPAATLELWYEHPTPGAHAGMPTLTDLTLPPDADIYLCGGPAFLQSARAQLAAIGIPTARIHFE
LFTPNDWLLD

Specific function: Is involved in NO detoxification in an aerobic process, termed nitric oxide dioxygenase (NOD) reaction that utilizes O(2) and NAD(P)H to convert NO to nitrate, which protects the bacterium from various noxious nitrogen compounds. Therefore, plays a centra

COG id: COG1017

COG function: function code C; Hemoglobin-like flavoprotein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding FR-type domain [H]

Homologues:

Organism=Escherichia coli, GI1788903, Length=409, Percent_Identity=32.518337408313, Blast_Score=196, Evalue=3e-51,
Organism=Escherichia coli, GI1788104, Length=241, Percent_Identity=29.045643153527, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1787098, Length=222, Percent_Identity=27.4774774774775, Blast_Score=67, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6321673, Length=399, Percent_Identity=33.3333333333333, Blast_Score=187, Evalue=2e-48,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017927
- InterPro:   IPR001709
- InterPro:   IPR012292
- InterPro:   IPR009050
- InterPro:   IPR000971
- InterPro:   IPR008333
- InterPro:   IPR001433
- InterPro:   IPR001221
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00970 FAD_binding_6; PF00042 Globin; PF00175 NAD_binding_1 [H]

EC number: =1.14.12.17 [H]

Molecular weight: Translated: 43686; Mature: 43555

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: PS01033 GLOBIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIVSPSALGAPAELEAHHAEVVRATLPVIAAHIDDITALFYRKLFTAHPELLANLFNRG
CCEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCC
NQAQGAQQRALAASIATFAAHLVDPALPHPADLLARIGHKHASLGVVAEQYPIVHEHLFA
CCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCHHCCHHHHCCHHHHHHHH
AIVAVLDADTVTPEVAAAWDRVYWLMADALIGFEGELYRAAGVAPGDVFRETVVVDRVED
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEHCCCCCCHHHHHHHHHEECCCC
PSGVVTFVVESADPAAPLPDFLPGQYVSVGARLLDGARQLRQYSLVAAPGGGRLAFAVRR
CCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHEEEEECCCCCEEEEEEEE
VEPTPEQPAGEVSTWLHTQVRPGDTLEITLPFGDLVVDTDAATPLVLISAGIGITPMIGI
CCCCCCCCCCHHHHHHEEECCCCCEEEEEECCCCEEEECCCCCCEEEEECCCCHHHHHHH
LEHLAAATPNRRVLAVHADRTPDTHPLRAVQADLIAALPAATLELWYEHPTPGAHAGMPT
HHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHCCHHHHHHEECCCCCCCCCCCCC
LTDLTLPPDADIYLCGGPAFLQSARAQLAAIGIPTARIHFELFTPNDWLLD
CEECCCCCCCCEEEECCHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCCCC
>Mature Secondary Structure 
TIVSPSALGAPAELEAHHAEVVRATLPVIAAHIDDITALFYRKLFTAHPELLANLFNRG
CEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCC
NQAQGAQQRALAASIATFAAHLVDPALPHPADLLARIGHKHASLGVVAEQYPIVHEHLFA
CCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCHHCCHHHHCCHHHHHHHH
AIVAVLDADTVTPEVAAAWDRVYWLMADALIGFEGELYRAAGVAPGDVFRETVVVDRVED
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEHCCCCCCHHHHHHHHHEECCCC
PSGVVTFVVESADPAAPLPDFLPGQYVSVGARLLDGARQLRQYSLVAAPGGGRLAFAVRR
CCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHEEEEECCCCCEEEEEEEE
VEPTPEQPAGEVSTWLHTQVRPGDTLEITLPFGDLVVDTDAATPLVLISAGIGITPMIGI
CCCCCCCCCCHHHHHHEEECCCCCEEEEEECCCCEEEECCCCCCEEEEECCCCHHHHHHH
LEHLAAATPNRRVLAVHADRTPDTHPLRAVQADLIAALPAATLELWYEHPTPGAHAGMPT
HHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHCCHHHHHHEECCCCCCCCCCCCC
LTDLTLPPDADIYLCGGPAFLQSARAQLAAIGIPTARIHFELFTPNDWLLD
CEECCCCCCCCEEEECCHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14521231; 11481432 [H]