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The map label for this gene is hisS

Identifier: 54025634

GI number: 54025634

Start: 3879518

End: 3880786

Strand: Reverse

Name: hisS

Synonym: nfa36640

Alternate gene names: 54025634

Gene position: 3880786-3879518 (Counterclockwise)

Preceding gene: 54025637

Following gene: 54025633

Centisome position: 64.45

GC content: 71.47

Gene sequence:

>1269_bases
GTGACCAAGACCAGCAGCTTCTCCGCCCCGAAGGGGGTACCGGACTACGTGCCACCCGGCTCGGCCGAGTTCGTCGCGGT
GCGCGACGCGCTCCTGCGGGCCGCCCGACTGGCCGGGTACGGGCATATCGAGCTGCCGATCTTCGAGGACACCGGCCTGT
TCGCGCGCGGCGTCGGCGAGTCCACCGACGTGGTGACCAAGGAGATGTACACCTTCGCCGATCGCGGCGAGCGCAGCGTC
ACGCTGCGGCCGGAGGGCACCGCGGGCGTGATGCGCGCGGTCATCGAGCACGGTCTCGACCGTGGCCAGCTGCCCGTCAA
GCTGGTCTACTCCGGTCCCTTCTTCCGCTACGAACGCCCGCAGGCGGGCCGCTACCGGCAGTTGCAGCAGGTGGGCGTGG
AGGCGATCGGCGTCGACGATCCGGCGCTGGACGCCGAGGTCATCGCCATCGCCGACGCGGGCTTCCGTTCGCTGGGCCTG
GACGGGTTCCGGCTGGAGCTGACCTCGTTGGGCGACGACACCTGCCGCCCGCGCTACCGCGAGCTGCTACAGGAGTTCCT
GTTCGGGCTGCCACTCGACGAGGAGACCCGCCGTCGCGCCGAGCTCAACCCGCTGCGCGTGCTCGACGACAAGCGCCCGG
AGGTGCGGGAGCTGCTCGCCGACGCGCCGCTGATGATCGACCACCTCTCCGAATCGGCCAAGGCCCACTTCGAGCAGGTG
CTCGGCCACCTCGACGCGCTGGGCGTGCCGTATGTGGTGAACCCGCGGATGGTGCGCGGCCTGGACTACTACACCAAGAC
CACCTTCGAGTTCGTGCACGACGGCCTCGGCGCGCAGTCCGGCATCGGCGGCGGCGGCCGCTACGACGGGCTGATGGCCC
AGCTCGGTGGCCAGCCGCTGTCGGGCATCGGCTTCGGGCTCGGCGTCGACCGCACCATGCTGGCCTTGCAGGCCGAGGGC
AAATCGGCGGGCGACCCGGCGCGCTGCGACGTCTTCGGGGTGCCGCTCGGTGAGGCCGCCAAGCAGCGAATGGTGGTGCT
GGCCGCCCAGCTGCGCGCCGCCGGCGTGCGGGTCGACCTCGCCTACGGCGGTCGGGGTGTCAAGGGTGCGATGAAGGCGG
CCGACCGCTCCGGCGCCCGCTACACCCTGGTGCTGGGCGACCGGGATCTGGCCGAGGACACCATCGGTGTCAAGGACATG
AGCACCGGGGACCAACGGCAGGTGCCGCTGGGCGAGGTCGTCGGCGTGCTGCGCAGCGAACTCGGATAG

Upstream 100 bases:

>100_bases
AGGTAAGAGTGTGCCATCCCTTCCTGAGAGGAGGGTCGGGTCGGCGCGATGGGGTAACCGGTTGGACTTGGCGGGCCGCG
GTGGGGAAAACTGGAACATC

Downstream 100 bases:

>100_bases
GTCCCGAGCGGGCGCACCCGCGGGGTACGGTTGCTGCGCACCACGGGTGCGGTGGCTGATGCGCACCACGGGTGCGGTGG
CGTGTGCGAAGAGGTGGAAC

Product: histidyl-tRNA synthetase

Products: NA

Alternate protein names: Histidine--tRNA ligase; HisRS

Number of amino acids: Translated: 422; Mature: 421

Protein sequence:

>422_residues
MTKTSSFSAPKGVPDYVPPGSAEFVAVRDALLRAARLAGYGHIELPIFEDTGLFARGVGESTDVVTKEMYTFADRGERSV
TLRPEGTAGVMRAVIEHGLDRGQLPVKLVYSGPFFRYERPQAGRYRQLQQVGVEAIGVDDPALDAEVIAIADAGFRSLGL
DGFRLELTSLGDDTCRPRYRELLQEFLFGLPLDEETRRRAELNPLRVLDDKRPEVRELLADAPLMIDHLSESAKAHFEQV
LGHLDALGVPYVVNPRMVRGLDYYTKTTFEFVHDGLGAQSGIGGGGRYDGLMAQLGGQPLSGIGFGLGVDRTMLALQAEG
KSAGDPARCDVFGVPLGEAAKQRMVVLAAQLRAAGVRVDLAYGGRGVKGAMKAADRSGARYTLVLGDRDLAEDTIGVKDM
STGDQRQVPLGEVVGVLRSELG

Sequences:

>Translated_422_residues
MTKTSSFSAPKGVPDYVPPGSAEFVAVRDALLRAARLAGYGHIELPIFEDTGLFARGVGESTDVVTKEMYTFADRGERSV
TLRPEGTAGVMRAVIEHGLDRGQLPVKLVYSGPFFRYERPQAGRYRQLQQVGVEAIGVDDPALDAEVIAIADAGFRSLGL
DGFRLELTSLGDDTCRPRYRELLQEFLFGLPLDEETRRRAELNPLRVLDDKRPEVRELLADAPLMIDHLSESAKAHFEQV
LGHLDALGVPYVVNPRMVRGLDYYTKTTFEFVHDGLGAQSGIGGGGRYDGLMAQLGGQPLSGIGFGLGVDRTMLALQAEG
KSAGDPARCDVFGVPLGEAAKQRMVVLAAQLRAAGVRVDLAYGGRGVKGAMKAADRSGARYTLVLGDRDLAEDTIGVKDM
STGDQRQVPLGEVVGVLRSELG
>Mature_421_residues
TKTSSFSAPKGVPDYVPPGSAEFVAVRDALLRAARLAGYGHIELPIFEDTGLFARGVGESTDVVTKEMYTFADRGERSVT
LRPEGTAGVMRAVIEHGLDRGQLPVKLVYSGPFFRYERPQAGRYRQLQQVGVEAIGVDDPALDAEVIAIADAGFRSLGLD
GFRLELTSLGDDTCRPRYRELLQEFLFGLPLDEETRRRAELNPLRVLDDKRPEVRELLADAPLMIDHLSESAKAHFEQVL
GHLDALGVPYVVNPRMVRGLDYYTKTTFEFVHDGLGAQSGIGGGGRYDGLMAQLGGQPLSGIGFGLGVDRTMLALQAEGK
SAGDPARCDVFGVPLGEAAKQRMVVLAAQLRAAGVRVDLAYGGRGVKGAMKAADRSGARYTLVLGDRDLAEDTIGVKDMS
TGDQRQVPLGEVVGVLRSELG

Specific function: Unknown

COG id: COG0124

COG function: function code J; Histidyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Escherichia coli, GI1788861, Length=424, Percent_Identity=44.1037735849057, Blast_Score=328, Evalue=4e-91,
Organism=Drosophila melanogaster, GI24643061, Length=470, Percent_Identity=23.6170212765957, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24643059, Length=486, Percent_Identity=23.2510288065844, Blast_Score=74, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYH_NOCFA (Q5YTH9)

Other databases:

- EMBL:   AP006618
- RefSeq:   YP_119876.1
- ProteinModelPortal:   Q5YTH9
- SMR:   Q5YTH9
- GeneID:   3109574
- GenomeReviews:   AP006618_GR
- KEGG:   nfa:nfa36640
- NMPDR:   fig|247156.1.peg.3574
- HOGENOM:   HBG616575
- OMA:   VFEWVTT
- ProtClustDB:   PRK00037
- BioCyc:   NFAR247156:NFA36640-MONOMER
- BRENDA:   6.1.1.21
- GO:   GO:0005737
- HAMAP:   MF_00127
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR015807
- InterPro:   IPR004516
- Gene3D:   G3DSA:3.40.50.800
- PANTHER:   PTHR11476
- PIRSF:   PIRSF001549
- TIGRFAMs:   TIGR00442

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; SSF52954 Anticodon_bd

EC number: =6.1.1.21

Molecular weight: Translated: 45477; Mature: 45346

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKTSSFSAPKGVPDYVPPGSAEFVAVRDALLRAARLAGYGHIELPIFEDTGLFARGVGE
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCEECCCC
STDVVTKEMYTFADRGERSVTLRPEGTAGVMRAVIEHGLDRGQLPVKLVYSGPFFRYERP
CHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHCCCCCCCCCEEEEECCCCEEECCC
QAGRYRQLQQVGVEAIGVDDPALDAEVIAIADAGFRSLGLDGFRLELTSLGDDTCRPRYR
CCHHHHHHHHCCCEEECCCCCCCCCEEEEEECCCHHHCCCCCEEEEEECCCCCCCCHHHH
ELLQEFLFGLPLDEETRRRAELNPLRVLDDKRPEVRELLADAPLMIDHLSESAKAHFEQV
HHHHHHHHCCCCCHHHHHHHCCCCCEECCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHH
LGHLDALGVPYVVNPRMVRGLDYYTKTTFEFVHDGLGAQSGIGGGGRYDGLMAQLGGQPL
HHHHHHCCCCEEECCHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCC
SGIGFGLGVDRTMLALQAEGKSAGDPARCDVFGVPLGEAAKQRMVVLAAQLRAAGVRVDL
CCCCCCCCCCCEEEEEEECCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHCCEEEEE
AYGGRGVKGAMKAADRSGARYTLVLGDRDLAEDTIGVKDMSTGDQRQVPLGEVVGVLRSE
EECCCCCHHHHHHHCCCCCEEEEEECCCCHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHH
LG
CC
>Mature Secondary Structure 
TKTSSFSAPKGVPDYVPPGSAEFVAVRDALLRAARLAGYGHIELPIFEDTGLFARGVGE
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCEECCCC
STDVVTKEMYTFADRGERSVTLRPEGTAGVMRAVIEHGLDRGQLPVKLVYSGPFFRYERP
CHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHCCCCCCCCCEEEEECCCCEEECCC
QAGRYRQLQQVGVEAIGVDDPALDAEVIAIADAGFRSLGLDGFRLELTSLGDDTCRPRYR
CCHHHHHHHHCCCEEECCCCCCCCCEEEEEECCCHHHCCCCCEEEEEECCCCCCCCHHHH
ELLQEFLFGLPLDEETRRRAELNPLRVLDDKRPEVRELLADAPLMIDHLSESAKAHFEQV
HHHHHHHHCCCCCHHHHHHHCCCCCEECCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHH
LGHLDALGVPYVVNPRMVRGLDYYTKTTFEFVHDGLGAQSGIGGGGRYDGLMAQLGGQPL
HHHHHHCCCCEEECCHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCC
SGIGFGLGVDRTMLALQAEGKSAGDPARCDVFGVPLGEAAKQRMVVLAAQLRAAGVRVDL
CCCCCCCCCCCEEEEEEECCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHCCEEEEE
AYGGRGVKGAMKAADRSGARYTLVLGDRDLAEDTIGVKDMSTGDQRQVPLGEVVGVLRSE
EECCCCCHHHHHHHCCCCCEEEEEECCCCHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHH
LG
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA