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The map label for this gene is cyoE

Identifier: 54025542

GI number: 54025542

Start: 3779657

End: 3780592

Strand: Reverse

Name: cyoE

Synonym: nfa35720

Alternate gene names: 54025542

Gene position: 3780592-3779657 (Counterclockwise)

Preceding gene: 54025550

Following gene: 54025540

Centisome position: 62.79

GC content: 69.55

Gene sequence:

>936_bases
GTGCTTGCCGACCGGTTCACCGGTTCGGGCCTGCCGTCCCGGCTGATGCGACGGGTGCTGGCCTACATCGCGTTGACCAA
GCCGCGGGTGATCGAGCTGCTGCTGGTGGCGACCATCCCGACCATGCTGCTGGCCGATCGCGGGCACATCGACATCCGGC
TGATCCTGGCCACGCTGTTCGGCGGGTGGATGGGCGCGGCGAGCGCCAACACGCTCAACTGCGTCGCCGACGCCGACATC
GACAAGGTCATGAAGCGCACCGCCAAGCGGCCGCTGGCCAGGGACGCGGTGCCCACCCGCAACGCGTTCGTCTTCGGCGT
GGTGCTGGGCCTGGCCTCCTTCGCCTGGCTGTGGTGGCAGGCGAACCTGCTCAGCGGCGCGCTGGTGGTCGCGACCATCC
TGTTCTACGTCTTCGTCTACACCCTCGGCCTCAAGCGCCGCACCTCGCAGAACGTGGTGTGGGGCGGCGCGGCGGGCTGC
ATGCCCGCGCTGGTCGGTTGGTCGGCGGTGACGGGCACCATCGGCTGGCCCGCGCTGGCGCTGTTCGGCGTGATCTTCTT
CTGGACGCCGCCGCACACCTGGGCGCTGGCGATGCGCTACAAGGAGGACTACCGCGCGGCGGGGGTGCCGATGCTGCCGG
TGGTCGCCACCGAGCGCACCGTCACCAAGCAGATCGTGATCTACACCTGGCTGACCGTGCTCACCACGCTCGCGCTGGTG
CCGGCCACCGGCGTGATCTACGCGGCGGTCGCGCTGGTCGCCGGCGCCTGGTTCCTGCTGATGGCCCACCAGCTGTACGC
GGGCGTGCGCCGTGGCGAGTCGGTCAAGCCGCTGCGGTTGTTCCTACAGTCCAACAACTACCTGGCCGTGGTGTTCTGCG
GTCTGGCCGTGGACTCGGTGCTCGGCTGGGACACCATCGGCAGCTTCTTCGGCTGA

Upstream 100 bases:

>100_bases
GCTTTTGTTCGGTGTCGATGCGATGCACAGCGTGCGAGGAGAGACAGTGCGGATTGGGGAACAGCCGAGTGGCAATGGTG
CGCACGGCTCCTCCGCGACG

Downstream 100 bases:

>100_bases
CCGACTCGTCTTCCGGCCGTTGCCGGGCGGCCGCCGGAACGGCGACCGCCCGCGTGTTCTCAGGGGATCAGCACGATCGA
ACCGGTGGTCTTGCGGCCCT

Product: protoheme IX farnesyltransferase

Products: heme O; pyrophosphate [C]

Alternate protein names: Heme B farnesyltransferase; Heme O synthase

Number of amino acids: Translated: 311; Mature: 311

Protein sequence:

>311_residues
MLADRFTGSGLPSRLMRRVLAYIALTKPRVIELLLVATIPTMLLADRGHIDIRLILATLFGGWMGAASANTLNCVADADI
DKVMKRTAKRPLARDAVPTRNAFVFGVVLGLASFAWLWWQANLLSGALVVATILFYVFVYTLGLKRRTSQNVVWGGAAGC
MPALVGWSAVTGTIGWPALALFGVIFFWTPPHTWALAMRYKEDYRAAGVPMLPVVATERTVTKQIVIYTWLTVLTTLALV
PATGVIYAAVALVAGAWFLLMAHQLYAGVRRGESVKPLRLFLQSNNYLAVVFCGLAVDSVLGWDTIGSFFG

Sequences:

>Translated_311_residues
MLADRFTGSGLPSRLMRRVLAYIALTKPRVIELLLVATIPTMLLADRGHIDIRLILATLFGGWMGAASANTLNCVADADI
DKVMKRTAKRPLARDAVPTRNAFVFGVVLGLASFAWLWWQANLLSGALVVATILFYVFVYTLGLKRRTSQNVVWGGAAGC
MPALVGWSAVTGTIGWPALALFGVIFFWTPPHTWALAMRYKEDYRAAGVPMLPVVATERTVTKQIVIYTWLTVLTTLALV
PATGVIYAAVALVAGAWFLLMAHQLYAGVRRGESVKPLRLFLQSNNYLAVVFCGLAVDSVLGWDTIGSFFG
>Mature_311_residues
MLADRFTGSGLPSRLMRRVLAYIALTKPRVIELLLVATIPTMLLADRGHIDIRLILATLFGGWMGAASANTLNCVADADI
DKVMKRTAKRPLARDAVPTRNAFVFGVVLGLASFAWLWWQANLLSGALVVATILFYVFVYTLGLKRRTSQNVVWGGAAGC
MPALVGWSAVTGTIGWPALALFGVIFFWTPPHTWALAMRYKEDYRAAGVPMLPVVATERTVTKQIVIYTWLTVLTTLALV
PATGVIYAAVALVAGAWFLLMAHQLYAGVRRGESVKPLRLFLQSNNYLAVVFCGLAVDSVLGWDTIGSFFG

Specific function: Converts heme B (protoheme IX) to heme O by substitution of the vinyl group on carbon 2 of heme B porphyrin ring with a hydroxyethyl farnesyl side group

COG id: COG0109

COG function: function code O; Polyprenyltransferase (cytochrome oxidase assembly factor)

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ubiA prenyltransferase family. Protoheme IX farnesyltransferase subfamily

Homologues:

Organism=Homo sapiens, GI17921982, Length=263, Percent_Identity=28.8973384030418, Blast_Score=84, Evalue=2e-16,
Organism=Escherichia coli, GI1786631, Length=247, Percent_Identity=34.8178137651822, Blast_Score=130, Evalue=9e-32,
Organism=Caenorhabditis elegans, GI71998235, Length=258, Percent_Identity=26.7441860465116, Blast_Score=73, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6325085, Length=289, Percent_Identity=25.2595155709343, Blast_Score=85, Evalue=1e-17,
Organism=Drosophila melanogaster, GI19921064, Length=264, Percent_Identity=27.2727272727273, Blast_Score=85, Evalue=6e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): COXX_NOCFA (Q5YTS1)

Other databases:

- EMBL:   AP006618
- RefSeq:   YP_119784.1
- GeneID:   3109791
- GenomeReviews:   AP006618_GR
- KEGG:   nfa:nfa35720
- NMPDR:   fig|247156.1.peg.3486
- HOGENOM:   HBG656003
- OMA:   IPHFMAL
- ProtClustDB:   PRK04375
- BioCyc:   NFAR247156:NFA35720-MONOMER
- HAMAP:   MF_00154
- InterPro:   IPR006369
- InterPro:   IPR000537
- PANTHER:   PTHR11048:SF3
- TIGRFAMs:   TIGR01473

Pfam domain/function: PF01040 UbiA

EC number: 2.5.1.- [C]

Molecular weight: Translated: 33867; Mature: 33867

Theoretical pI: Translated: 10.47; Mature: 10.47

Prosite motif: PS00943 UBIA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x19f9548c)-; HASH(0x40c30174)-; HASH(0x195857b8)-; HASH(0x1ad610e4)-; HASH(0x409e1bb8)-; HASH(0x1ad323fc)-; HASH(0x19fa6f18)-; HASH(0x409c10a4)-;

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLADRFTGSGLPSRLMRRVLAYIALTKPRVIELLLVATIPTMLLADRGHIDIRLILATLF
CCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
GGWMGAASANTLNCVADADIDKVMKRTAKRPLARDAVPTRNAFVFGVVLGLASFAWLWWQ
HHHHCCCCCCCEEEEECCCHHHHHHHHHHCCCHHCCCCCCHHHHHHHHHHHHHHHHHHHH
ANLLSGALVVATILFYVFVYTLGLKRRTSQNVVWGGAAGCMPALVGWSAVTGTIGWPALA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHHHHCCCCHHHHH
LFGVIFFWTPPHTWALAMRYKEDYRAAGVPMLPVVATERTVTKQIVIYTWLTVLTTLALV
HHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHH
PATGVIYAAVALVAGAWFLLMAHQLYAGVRRGESVKPLRLFLQSNNYLAVVFCGLAVDSV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHEEECCCEEEEEEHHHHHHHH
LGWDTIGSFFG
HHHHHHHHHCH
>Mature Secondary Structure
MLADRFTGSGLPSRLMRRVLAYIALTKPRVIELLLVATIPTMLLADRGHIDIRLILATLF
CCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
GGWMGAASANTLNCVADADIDKVMKRTAKRPLARDAVPTRNAFVFGVVLGLASFAWLWWQ
HHHHCCCCCCCEEEEECCCHHHHHHHHHHCCCHHCCCCCCHHHHHHHHHHHHHHHHHHHH
ANLLSGALVVATILFYVFVYTLGLKRRTSQNVVWGGAAGCMPALVGWSAVTGTIGWPALA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHHHHCCCCHHHHH
LFGVIFFWTPPHTWALAMRYKEDYRAAGVPMLPVVATERTVTKQIVIYTWLTVLTTLALV
HHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHH
PATGVIYAAVALVAGAWFLLMAHQLYAGVRRGESVKPLRLFLQSNNYLAVVFCGLAVDSV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHEEECCCEEEEEEHHHHHHHH
LGWDTIGSFFG
HHHHHHHHHCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protoheme; farnesyl-PP [C]

Specific reaction: protoheme + farnesyl-PP = heme O + pyrophosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA