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The map label for this gene is mutB [H]

Identifier: 54025436

GI number: 54025436

Start: 3673972

End: 3676254

Strand: Reverse

Name: mutB [H]

Synonym: nfa34660

Alternate gene names: 54025436

Gene position: 3676254-3673972 (Counterclockwise)

Preceding gene: 54025437

Following gene: 54025435

Centisome position: 61.05

GC content: 68.68

Gene sequence:

>2283_bases
ATGACCCTCAGTCACATCGAAGAACCGGGCGACGTGGCCCATCTGGTGGGCAACTTCGCCGAGGTGCCGCTGACCGAGCA
CGATCCCGAGCCCGCCGCGGTGGACGCCGCGCAGGTCGAGGAGTTCGTGCGCACGGCCGCCGCGGCCAACAATTACACCC
CCGAGCAGTTGACCTGGTCGACCCCGGAGGGCATCGACGTGCCGCCGGTGTTCACCAAGGCCGACCGGGACGCCGTCGCC
GCGGCGGGCTACCCGCTCGACAGCGTGCCGGGTGTGGCGCCGTTCGTGCGCGGCCCGTACCCGACCATGTATGTCAACCA
GCCGTGGACCATCCGCCAGTACGCGGGCTTCTCCACGGCCGCCGACTCCAACGCCTTCTACCGTCGCAACCTGCAGGCGG
GCCAGAAGGGTCTGTCGGTCGCCTTCGACCTGGCCACCCACCGTGGCTACGACTCCGATCACCCGCGCGTGCAGGGTGAT
GTCGGCATGGCCGGCGTGGCGATCGACTCGATCCTGGACATGCGCCAGCTCTTCGATCACATCCCGCTGGACCAGGTCTC
GGTGTCGATGACCATGAACGGCGCGGTGTTGCCGATCCTGGCGCTGTACGTGGTGGCCGCCGAGGAGCAGGGCGTCAAGC
CCGAGCAGCTGGCCGGAACGATTCAGAACGACATTCTGAAGGAGTTCATGGTCCGCAACACCTACATCTACCCGCCCAAG
CCCTCGATGCGGATCATCTCCGACATCTTCGCCTACACCAGCGCGAAGATGCCGAAGTTCAACTCGATCTCCATCTCCGG
CTACCACATCCAGGAGGCGGGCGCGACCGCCGACCTGGAACTCGCCTACACCCTCGCCGACGGCGTGGAGTACATCCGGG
CCGGTATCGACGCGGGCATGGAGGTCGACAAGTTCGCCCCCCGGCTGTCGTTCTTCTGGGCGATCGGCATGAACTTCTTC
ATGGAGGTCGCCAAGCTGCGCGCGGGCCGGCTGCTGTGGAGCGAGCTGGTGTCGAAGTTCGAGCCGAAGAACTCGAAATC
GCTGTCGCTGCGCACCCATTCGCAGACCTCCGGCTGGTCGCTGACCGCCCAGGACGTCTACAACAACGTGGCGCGCACCT
GCATCGAGGCGATGGCCGCCACCCAGGGCCACACCCAGTCCCTGCACACCAACGCCCTGGACGAGGCGCTGGCGCTGCCG
ACCGACTTCTCGGCCCGCATCGCCCGCAACACCCAGCTGCTGATCCAGCAGGAGTCCAACACCACCCGGCCGATCGACCC
GTGGGGCGGCTCCTACTACGTCGAATGGCTGACCCATCAGCTGGCCGAGCGCGCCCGCGCGCACATCGCCGAGATCGAGG
CGCACGGCGGTATGGCGCAGGCGATCTCGGAGGGCATCCCGAAGCTGCGCATCGAGGAGGCCGCCGCCCGCACCCAGGCG
CGCATCGACACCGGCCAGCAGCCGGTGATCGGCGTCAACAAGTACCAGGTCGAGGAGGACCAGGCGGTCGAGGTCCTCAA
GGTCGAGAACTCCCGCGTGCGCGCCGAGCAGATCGAGAAGCTGCGCCGCCTGCGCGCCGAGCGCGACCAGGACGCCGTCG
ACCGTGCCCTGGCCGAGCTCACCCGCGCCGCCGCCGCCACCCAGGGCGGTATGGAGAACAACCTGCTGGCCCTGGCCATC
GACGCCGCCCGCGCCAAGGCCACCGTCGGGGAGATCTCCGACGCGCTGGAGAAGGTCTACGGCCGCCACCAGGCCGAGAT
CCGTACCCTGTCGGGTGTGTACCGGGACGAGGCCGGAAAGGTCACCAACATCACCATCGCCGGCAAGCTGGTCGAGGAGT
TCGCCGAGGCCGAGGGCAGGCGTCCCCGCATCCTCGTCGCCAAGATGGGTCAGGACGGGCACGACCGAGGCCAGAAGGTG
ATCGCCACCGCCTTCGCCGACCTCGGCTTCGACGTCGATGTGGGCCCGCTGTTCCAGACCCCCGAGGAGGTGGCGCAGCA
GGCGGCCGACAACGACGTCCACATCGTCGGTGTCTCGTCGCTGGCCGCGGGCCATCTCACGCTGGTGCCCGCCCTGCGGC
AGGCACTGGCCGATGTCGGACGCCCCGACATCATGGTCGTGGTCGGCGGCGTCATCCCGCCCGGTGACTTCGACGAGCTG
TACCAGGCGGGCGCCGCGGCGATCTTCCCGCCCGGCACCGTCATCGCCGACGCCGCCATCGACCTGCTGCGCAAGCTCGC
CGGCGAGCTGGGTCACGAACTGGAGGGTTCCGCGGCGGAATGA

Upstream 100 bases:

>100_bases
TCGCCGACCTCGACGCGGCGCAGCGCCCGGACGGCTTCCTCACCGCGAAGATCGACGCGGTGGCGCAACTGTCCGGCCTG
CTGGAGAAGGTGGGAGCCTG

Downstream 100 bases:

>100_bases
GCGACCCGGGTTCCGCCGCGCCCACCCGGGCGCGGCGGAAGATCGACGTAGACGAACTCGCCGCCGCCGTCCGGCGCAAC
GACCGAGCGGCCCTGGCACG

Product: methylmalonyl-CoA mutase

Products: NA

Alternate protein names: MCM [H]

Number of amino acids: Translated: 760; Mature: 759

Protein sequence:

>760_residues
MTLSHIEEPGDVAHLVGNFAEVPLTEHDPEPAAVDAAQVEEFVRTAAAANNYTPEQLTWSTPEGIDVPPVFTKADRDAVA
AAGYPLDSVPGVAPFVRGPYPTMYVNQPWTIRQYAGFSTAADSNAFYRRNLQAGQKGLSVAFDLATHRGYDSDHPRVQGD
VGMAGVAIDSILDMRQLFDHIPLDQVSVSMTMNGAVLPILALYVVAAEEQGVKPEQLAGTIQNDILKEFMVRNTYIYPPK
PSMRIISDIFAYTSAKMPKFNSISISGYHIQEAGATADLELAYTLADGVEYIRAGIDAGMEVDKFAPRLSFFWAIGMNFF
MEVAKLRAGRLLWSELVSKFEPKNSKSLSLRTHSQTSGWSLTAQDVYNNVARTCIEAMAATQGHTQSLHTNALDEALALP
TDFSARIARNTQLLIQQESNTTRPIDPWGGSYYVEWLTHQLAERARAHIAEIEAHGGMAQAISEGIPKLRIEEAAARTQA
RIDTGQQPVIGVNKYQVEEDQAVEVLKVENSRVRAEQIEKLRRLRAERDQDAVDRALAELTRAAAATQGGMENNLLALAI
DAARAKATVGEISDALEKVYGRHQAEIRTLSGVYRDEAGKVTNITIAGKLVEEFAEAEGRRPRILVAKMGQDGHDRGQKV
IATAFADLGFDVDVGPLFQTPEEVAQQAADNDVHIVGVSSLAAGHLTLVPALRQALADVGRPDIMVVVGGVIPPGDFDEL
YQAGAAAIFPPGTVIADAAIDLLRKLAGELGHELEGSAAE

Sequences:

>Translated_760_residues
MTLSHIEEPGDVAHLVGNFAEVPLTEHDPEPAAVDAAQVEEFVRTAAAANNYTPEQLTWSTPEGIDVPPVFTKADRDAVA
AAGYPLDSVPGVAPFVRGPYPTMYVNQPWTIRQYAGFSTAADSNAFYRRNLQAGQKGLSVAFDLATHRGYDSDHPRVQGD
VGMAGVAIDSILDMRQLFDHIPLDQVSVSMTMNGAVLPILALYVVAAEEQGVKPEQLAGTIQNDILKEFMVRNTYIYPPK
PSMRIISDIFAYTSAKMPKFNSISISGYHIQEAGATADLELAYTLADGVEYIRAGIDAGMEVDKFAPRLSFFWAIGMNFF
MEVAKLRAGRLLWSELVSKFEPKNSKSLSLRTHSQTSGWSLTAQDVYNNVARTCIEAMAATQGHTQSLHTNALDEALALP
TDFSARIARNTQLLIQQESNTTRPIDPWGGSYYVEWLTHQLAERARAHIAEIEAHGGMAQAISEGIPKLRIEEAAARTQA
RIDTGQQPVIGVNKYQVEEDQAVEVLKVENSRVRAEQIEKLRRLRAERDQDAVDRALAELTRAAAATQGGMENNLLALAI
DAARAKATVGEISDALEKVYGRHQAEIRTLSGVYRDEAGKVTNITIAGKLVEEFAEAEGRRPRILVAKMGQDGHDRGQKV
IATAFADLGFDVDVGPLFQTPEEVAQQAADNDVHIVGVSSLAAGHLTLVPALRQALADVGRPDIMVVVGGVIPPGDFDEL
YQAGAAAIFPPGTVIADAAIDLLRKLAGELGHELEGSAAE
>Mature_759_residues
TLSHIEEPGDVAHLVGNFAEVPLTEHDPEPAAVDAAQVEEFVRTAAAANNYTPEQLTWSTPEGIDVPPVFTKADRDAVAA
AGYPLDSVPGVAPFVRGPYPTMYVNQPWTIRQYAGFSTAADSNAFYRRNLQAGQKGLSVAFDLATHRGYDSDHPRVQGDV
GMAGVAIDSILDMRQLFDHIPLDQVSVSMTMNGAVLPILALYVVAAEEQGVKPEQLAGTIQNDILKEFMVRNTYIYPPKP
SMRIISDIFAYTSAKMPKFNSISISGYHIQEAGATADLELAYTLADGVEYIRAGIDAGMEVDKFAPRLSFFWAIGMNFFM
EVAKLRAGRLLWSELVSKFEPKNSKSLSLRTHSQTSGWSLTAQDVYNNVARTCIEAMAATQGHTQSLHTNALDEALALPT
DFSARIARNTQLLIQQESNTTRPIDPWGGSYYVEWLTHQLAERARAHIAEIEAHGGMAQAISEGIPKLRIEEAAARTQAR
IDTGQQPVIGVNKYQVEEDQAVEVLKVENSRVRAEQIEKLRRLRAERDQDAVDRALAELTRAAAATQGGMENNLLALAID
AARAKATVGEISDALEKVYGRHQAEIRTLSGVYRDEAGKVTNITIAGKLVEEFAEAEGRRPRILVAKMGQDGHDRGQKVI
ATAFADLGFDVDVGPLFQTPEEVAQQAADNDVHIVGVSSLAAGHLTLVPALRQALADVGRPDIMVVVGGVIPPGDFDELY
QAGAAAIFPPGTVIADAAIDLLRKLAGELGHELEGSAAE

Specific function: Catalyzes the isomerization of succinyl-CoA to methylmalonyl-CoA during synthesis of propionate from tricarboxylic acid-cycle intermediates [H]

COG id: COG1884

COG function: function code I; Methylmalonyl-CoA mutase, N-terminal domain/subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 B12-binding domain [H]

Homologues:

Organism=Homo sapiens, GI156105689, Length=692, Percent_Identity=64.8843930635838, Blast_Score=940, Evalue=0.0,
Organism=Escherichia coli, GI1789284, Length=699, Percent_Identity=60.6580829756795, Blast_Score=837, Evalue=0.0,
Organism=Caenorhabditis elegans, GI25144500, Length=697, Percent_Identity=63.4146341463415, Blast_Score=923, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006159
- InterPro:   IPR016176
- InterPro:   IPR014348
- InterPro:   IPR006158
- InterPro:   IPR006099
- InterPro:   IPR006098 [H]

Pfam domain/function: PF02310 B12-binding; PF01642 MM_CoA_mutase [H]

EC number: =5.4.99.2 [H]

Molecular weight: Translated: 82351; Mature: 82220

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: PS00544 METMALONYL_COA_MUTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLSHIEEPGDVAHLVGNFAEVPLTEHDPEPAAVDAAQVEEFVRTAAAANNYTPEQLTWS
CCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEC
TPEGIDVPPVFTKADRDAVAAAGYPLDSVPGVAPFVRGPYPTMYVNQPWTIRQYAGFSTA
CCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCHHCCCCCEEEECCCEEEHHHCCCCCC
ADSNAFYRRNLQAGQKGLSVAFDLATHRGYDSDHPRVQGDVGMAGVAIDSILDMRQLFDH
CCCCHHHHHHHHHCCCCCCEEEEHHHHCCCCCCCCEECCCCCHHHHHHHHHHHHHHHHHC
IPLDQVSVSMTMNGAVLPILALYVVAAEEQGVKPEQLAGTIQNDILKEFMVRNTYIYPPK
CCCCCEEEEEEECCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCEEECCC
PSMRIISDIFAYTSAKMPKFNSISISGYHIQEAGATADLELAYTLADGVEYIRAGIDAGM
CHHHHHHHHHHHHHCCCCCCCEEEEECEEECCCCCCCCEEEEHHHHHHHHHHHHHHCCCC
EVDKFAPRLSFFWAIGMNFFMEVAKLRAGRLLWSELVSKFEPKNSKSLSLRTHSQTSGWS
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCE
LTAQDVYNNVARTCIEAMAATQGHTQSLHTNALDEALALPTDFSARIARNTQLLIQQESN
EEHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCHHHHHCCCEEEEEECCC
TTRPIDPWGGSYYVEWLTHQLAERARAHIAEIEAHGGMAQAISEGIPKLRIEEAAARTQA
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCEEHHHHHHHHHH
RIDTGQQPVIGVNKYQVEEDQAVEVLKVENSRVRAEQIEKLRRLRAERDQDAVDRALAEL
HHCCCCCCEECCCCEECCCHHHEEEEEECCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHH
TRAAAATQGGMENNLLALAIDAARAKATVGEISDALEKVYGRHQAEIRTLSGVYRDEAGK
HHHHHHHCCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCC
VTNITIAGKLVEEFAEAEGRRPRILVAKMGQDGHDRGQKVIATAFADLGFDVDVGPLFQT
EEEEEEHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCC
PEEVAQQAADNDVHIVGVSSLAAGHLTLVPALRQALADVGRPDIMVVVGGVIPPGDFDEL
HHHHHHHHCCCCEEEEEEHHHCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHH
YQAGAAAIFPPGTVIADAAIDLLRKLAGELGHELEGSAAE
HHCCCCEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
TLSHIEEPGDVAHLVGNFAEVPLTEHDPEPAAVDAAQVEEFVRTAAAANNYTPEQLTWS
CCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEC
TPEGIDVPPVFTKADRDAVAAAGYPLDSVPGVAPFVRGPYPTMYVNQPWTIRQYAGFSTA
CCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCHHCCCCCEEEECCCEEEHHHCCCCCC
ADSNAFYRRNLQAGQKGLSVAFDLATHRGYDSDHPRVQGDVGMAGVAIDSILDMRQLFDH
CCCCHHHHHHHHHCCCCCCEEEEHHHHCCCCCCCCEECCCCCHHHHHHHHHHHHHHHHHC
IPLDQVSVSMTMNGAVLPILALYVVAAEEQGVKPEQLAGTIQNDILKEFMVRNTYIYPPK
CCCCCEEEEEEECCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCEEECCC
PSMRIISDIFAYTSAKMPKFNSISISGYHIQEAGATADLELAYTLADGVEYIRAGIDAGM
CHHHHHHHHHHHHHCCCCCCCEEEEECEEECCCCCCCCEEEEHHHHHHHHHHHHHHCCCC
EVDKFAPRLSFFWAIGMNFFMEVAKLRAGRLLWSELVSKFEPKNSKSLSLRTHSQTSGWS
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCE
LTAQDVYNNVARTCIEAMAATQGHTQSLHTNALDEALALPTDFSARIARNTQLLIQQESN
EEHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCHHHHHCCCEEEEEECCC
TTRPIDPWGGSYYVEWLTHQLAERARAHIAEIEAHGGMAQAISEGIPKLRIEEAAARTQA
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCEEHHHHHHHHHH
RIDTGQQPVIGVNKYQVEEDQAVEVLKVENSRVRAEQIEKLRRLRAERDQDAVDRALAEL
HHCCCCCCEECCCCEECCCHHHEEEEEECCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHH
TRAAAATQGGMENNLLALAIDAARAKATVGEISDALEKVYGRHQAEIRTLSGVYRDEAGK
HHHHHHHCCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCC
VTNITIAGKLVEEFAEAEGRRPRILVAKMGQDGHDRGQKVIATAFADLGFDVDVGPLFQT
EEEEEEHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCC
PEEVAQQAADNDVHIVGVSSLAAGHLTLVPALRQALADVGRPDIMVVVGGVIPPGDFDEL
HHHHHHHHCCCCEEEEEEHHHCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHH
YQAGAAAIFPPGTVIADAAIDLLRKLAGELGHELEGSAAE
HHCCCCEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]