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The map label for this gene is thrS

Identifier: 54020620

GI number: 54020620

Start: 759944

End: 761683

Strand: Reverse

Name: thrS

Synonym: mhp608

Alternate gene names: 54020620

Gene position: 761683-759944 (Counterclockwise)

Preceding gene: 54020621

Following gene: 54020619

Centisome position: 85.32

GC content: 28.97

Gene sequence:

>1740_bases
ATGGACTTAAAAATTGACTATAAATTAAATCATTCAAGTTCACACCTCTTAGCATTAGCAATTAAAATTCTTTATCCGGA
TGTAAAATTTGGAATTGGCCCTGTTTTTGATGATGGTTTTTATTATGATTTTGATCTTTCAGTCTCAATTTCACAATCTG
ATTTTCCAAAAATTGAGAAATTAATGAAAAAGTTAGTTTCAAAAAATCTAAAAATTGTTGAAACCGATGGGAAAAACCTT
GATTTTCAAAATCAAATTTATAAAGCGGAATTAAAAAATGATCTAGAAAAAAAAGGTGAAAAAATCACTTATTTTTCAAT
AGTTGATAGTAAAAACAACATACTTTTTGAGGATTTATGTTATGGAAATCATCTTGAATCTCTAGGAAAAATTCAAAATT
TTAAACTTTTAAAAATTGCCGGCGCTTATTGAAAAGGTGATTCTAAAAACAAACAGTTAACCCGAATTTATGGAACTAGT
TGAGATTCAAAAGAAAATCTTGATAAATTTCTTGGAATTTTACAAGAAAGACAAGAAAGAGATCATCGAAAAATCGGGGC
AAAATTAAAACTGTTTACATTTAGTTCTTATTTTGGCCATGGTTTTCCAGTTTGACTTGAAAATGGAATGCGAATTTATA
ATAAAATTCGTCAAAAAATTCTTGAATTTGACCGTAATTACGGATTTAAAGAGGTTCTTACCCCACATTTTGGCCATAAG
GAATTATATAAAATTTCCGGACATTTATTACATTATCGCGATGATATGTTTAAAGGCTTAAAAATTGAAAATGAGGAGTT
AATTCCGCGTCCGATGACTTGTCCACATCATATTATCCTTTTTTCACAGCAACTTTTTAGTTATCGAAATTTACCATATC
GAATCTCTGAACAATCACGATTATATCGTTATGAAAAATCAGGAGCACTTTTAGGCCTTGAACGCGTTCGAGCTATGGAT
TTAACGGAAGGGCATATTTTTGTTAGTAAAAGTCAAATTTTTAGTGAAATTAGTCATCTTTTTCAAATGATTTCAGAGGT
ACTTGATTTTTTTAAGGTCCAAATTTTTTATATCTCATTTTCAAAAAGAGACCCTAAAAACAAGGATAAATTTTTTCAAG
ATGATCAAATGTGGGATCAGGCAGAAGCTGATTTAAAGCAATTTTTAGAACAAAATAAAATTAATTATATAGAAAAAATC
GGTGAAGCTGCTTTTTATGGTCCCAAAATTGACTTCCAAGTTAAAACTGCACTAAATAAAGAGTTAACAATTTCAACTTT
ACAACTTGATTTCCTTTTACCTAAAAAATTTAAAATTTCCTTTATAAATGAGCATAATAAAAAAGAAACTCCAATTTTAA
TTCACCGTGGTTTAATAGGCACTTATGAACGGTTTATAGCGCTTTTATTAGAACAAAGTAAGGGAAATTTACCTTTCTGA
ATCTCGCCAAAACAGATAATTGTTATCCCAGTGGCTGAAAAACATAATGATTTTGCCCAAAAAATTCATAAAATTCTTTT
TGATCTTGGATTTAATTCGGAAATTGATCTCAGATCTGAACGCATTTCCCGTAAAATCCGGGAGGCAAATTTAGAAAAAA
TAAATTTTCAGGTAATAATCGGGGATGAGGAAATCACAAAAAACGAAATTACCTATAGAAAATTTGGTTCTCCTGAATTA
TTTAGAATTTCCTTAGTTGATTTTGTCGATTTGTTGAAAAGCAGCGAAAAAAATGTATAA

Upstream 100 bases:

>100_bases
CGATTTAGTCGAGATTTATGCAAAAAAAGGCGCTAAAAAAGCAAAAGAAGTTGCTGTTTCTAACTTTGGAAAAATCAAAG
AAAAAATAGGATTATAATTT

Downstream 100 bases:

>100_bases
AAAAATAACAAAAATTCCTGATTTTTTATTTGCATTGCTTACAATAATTCTTCCGATTAGCCTTATTTATGCATTTTTTT
CACCTGATTTTTATGGAAAA

Product: threonyl-tRNA synthetase

Products: NA

Alternate protein names: Threonine--tRNA ligase; ThrRS [H]

Number of amino acids: Translated: 579; Mature: 579

Protein sequence:

>579_residues
MDLKIDYKLNHSSSHLLALAIKILYPDVKFGIGPVFDDGFYYDFDLSVSISQSDFPKIEKLMKKLVSKNLKIVETDGKNL
DFQNQIYKAELKNDLEKKGEKITYFSIVDSKNNILFEDLCYGNHLESLGKIQNFKLLKIAGAYWKGDSKNKQLTRIYGTS
WDSKENLDKFLGILQERQERDHRKIGAKLKLFTFSSYFGHGFPVWLENGMRIYNKIRQKILEFDRNYGFKEVLTPHFGHK
ELYKISGHLLHYRDDMFKGLKIENEELIPRPMTCPHHIILFSQQLFSYRNLPYRISEQSRLYRYEKSGALLGLERVRAMD
LTEGHIFVSKSQIFSEISHLFQMISEVLDFFKVQIFYISFSKRDPKNKDKFFQDDQMWDQAEADLKQFLEQNKINYIEKI
GEAAFYGPKIDFQVKTALNKELTISTLQLDFLLPKKFKISFINEHNKKETPILIHRGLIGTYERFIALLLEQSKGNLPFW
ISPKQIIVIPVAEKHNDFAQKIHKILFDLGFNSEIDLRSERISRKIREANLEKINFQVIIGDEEITKNEITYRKFGSPEL
FRISLVDFVDLLKSSEKNV

Sequences:

>Translated_579_residues
MDLKIDYKLNHSSSHLLALAIKILYPDVKFGIGPVFDDGFYYDFDLSVSISQSDFPKIEKLMKKLVSKNLKIVETDGKNL
DFQNQIYKAELKNDLEKKGEKITYFSIVDSKNNILFEDLCYGNHLESLGKIQNFKLLKIAGAY*KGDSKNKQLTRIYGTS
*DSKENLDKFLGILQERQERDHRKIGAKLKLFTFSSYFGHGFPV*LENGMRIYNKIRQKILEFDRNYGFKEVLTPHFGHK
ELYKISGHLLHYRDDMFKGLKIENEELIPRPMTCPHHIILFSQQLFSYRNLPYRISEQSRLYRYEKSGALLGLERVRAMD
LTEGHIFVSKSQIFSEISHLFQMISEVLDFFKVQIFYISFSKRDPKNKDKFFQDDQMWDQAEADLKQFLEQNKINYIEKI
GEAAFYGPKIDFQVKTALNKELTISTLQLDFLLPKKFKISFINEHNKKETPILIHRGLIGTYERFIALLLEQSKGNLPF*
ISPKQIIVIPVAEKHNDFAQKIHKILFDLGFNSEIDLRSERISRKIREANLEKINFQVIIGDEEITKNEITYRKFGSPEL
FRISLVDFVDLLKSSEKNV
>Mature_579_residues
MDLKIDYKLNHSSSHLLALAIKILYPDVKFGIGPVFDDGFYYDFDLSVSISQSDFPKIEKLMKKLVSKNLKIVETDGKNL
DFQNQIYKAELKNDLEKKGEKITYFSIVDSKNNILFEDLCYGNHLESLGKIQNFKLLKIAGAY*KGDSKNKQLTRIYGTS
*DSKENLDKFLGILQERQERDHRKIGAKLKLFTFSSYFGHGFPV*LENGMRIYNKIRQKILEFDRNYGFKEVLTPHFGHK
ELYKISGHLLHYRDDMFKGLKIENEELIPRPMTCPHHIILFSQQLFSYRNLPYRISEQSRLYRYEKSGALLGLERVRAMD
LTEGHIFVSKSQIFSEISHLFQMISEVLDFFKVQIFYISFSKRDPKNKDKFFQDDQMWDQAEADLKQFLEQNKINYIEKI
GEAAFYGPKIDFQVKTALNKELTISTLQLDFLLPKKFKISFINEHNKKETPILIHRGLIGTYERFIALLLEQSKGNLPF*
ISPKQIIVIPVAEKHNDFAQKIHKILFDLGFNSEIDLRSERISRKIREANLEKINFQVIIGDEEITKNEITYRKFGSPEL
FRISLVDFVDLLKSSEKNV

Specific function: Thrs Is Also A Translational Repressor Protein, It Controls The Translation Of Its Own Gene By Binding To Its mRNA. [C]

COG id: COG0441

COG function: function code J; Threonyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI38202255, Length=551, Percent_Identity=33.5753176043557, Blast_Score=294, Evalue=1e-79,
Organism=Homo sapiens, GI38570111, Length=577, Percent_Identity=32.5823223570191, Blast_Score=280, Evalue=2e-75,
Organism=Homo sapiens, GI20070344, Length=567, Percent_Identity=31.7460317460317, Blast_Score=259, Evalue=4e-69,
Organism=Escherichia coli, GI1788013, Length=574, Percent_Identity=33.7979094076655, Blast_Score=357, Evalue=1e-99,
Organism=Caenorhabditis elegans, GI71984192, Length=545, Percent_Identity=33.7614678899083, Blast_Score=301, Evalue=6e-82,
Organism=Caenorhabditis elegans, GI71984184, Length=545, Percent_Identity=33.7614678899083, Blast_Score=301, Evalue=7e-82,
Organism=Saccharomyces cerevisiae, GI6322112, Length=560, Percent_Identity=33.5714285714286, Blast_Score=315, Evalue=1e-86,
Organism=Saccharomyces cerevisiae, GI6322654, Length=369, Percent_Identity=36.8563685636856, Blast_Score=224, Evalue=3e-59,
Organism=Drosophila melanogaster, GI24583842, Length=569, Percent_Identity=33.2161687170475, Blast_Score=306, Evalue=2e-83,
Organism=Drosophila melanogaster, GI28574141, Length=569, Percent_Identity=33.2161687170475, Blast_Score=306, Evalue=2e-83,
Organism=Drosophila melanogaster, GI24583839, Length=569, Percent_Identity=33.2161687170475, Blast_Score=306, Evalue=3e-83,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002320
- InterPro:   IPR018163
- InterPro:   IPR012947 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; PF07973 tRNA_SAD [H]

EC number: =6.1.1.3 [H]

Molecular weight: Translated: 67239; Mature: 67239

Theoretical pI: Translated: 9.48; Mature: 9.48

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLKIDYKLNHSSSHLLALAIKILYPDVKFGIGPVFDDGFYYDFDLSVSISQSDFPKIEK
CCEEEEEEECCCCCHHHHHHHHHHCCCHHCCCCCEECCCEEEEEEEEEEECCCCCHHHHH
LMKKLVSKNLKIVETDGKNLDFQNQIYKAELKNDLEKKGEKITYFSIVDSKNNILFEDLC
HHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCEEEEHHH
YGNHLESLGKIQNFKLLKIAGAYKGDSKNKQLTRIYGTSDSKENLDKFLGILQERQERDH
CCCHHHHHHHHCCEEEEEEECCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHH
RKIGAKLKLFTFSSYFGHGFPVLENGMRIYNKIRQKILEFDRNYGFKEVLTPHFGHKELY
HHHCCEEEEEEEEHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCHHHH
KISGHLLHYRDDMFKGLKIENEELIPRPMTCPHHIILFSQQLFSYRNLPYRISEQSRLYR
HHCCCEEEEHHHHHCCCEECCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEECCHHHHHE
YEKSGALLGLERVRAMDLTEGHIFVSKSQIFSEISHLFQMISEVLDFFKVQIFYISFSKR
ECCCCCEEEHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEECCC
DPKNKDKFFQDDQMWDQAEADLKQFLEQNKINYIEKIGEAAFYGPKIDFQVKTALNKELT
CCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCEEEEECCCCCEE
ISTLQLDFLLPKKFKISFINEHNKKETPILIHRGLIGTYERFIALLLEQSKGNLPFISPK
EEEEEEEEECCCCEEEEEECCCCCCCCCEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCC
QIIVIPVAEKHNDFAQKIHKILFDLGFNSEIDLRSERISRKIREANLEKINFQVIIGDEE
CEEEEEECCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCEEEEEEEEECCCC
ITKNEITYRKFGSPELFRISLVDFVDLLKSSEKNV
CCCCCCEEEECCCCCEEEEHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MDLKIDYKLNHSSSHLLALAIKILYPDVKFGIGPVFDDGFYYDFDLSVSISQSDFPKIEK
CCEEEEEEECCCCCHHHHHHHHHHCCCHHCCCCCEECCCEEEEEEEEEEECCCCCHHHHH
LMKKLVSKNLKIVETDGKNLDFQNQIYKAELKNDLEKKGEKITYFSIVDSKNNILFEDLC
HHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCEEEEHHH
YGNHLESLGKIQNFKLLKIAGAYKGDSKNKQLTRIYGTSDSKENLDKFLGILQERQERDH
CCCHHHHHHHHCCEEEEEEECCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHH
RKIGAKLKLFTFSSYFGHGFPVLENGMRIYNKIRQKILEFDRNYGFKEVLTPHFGHKELY
HHHCCEEEEEEEEHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCHHHH
KISGHLLHYRDDMFKGLKIENEELIPRPMTCPHHIILFSQQLFSYRNLPYRISEQSRLYR
HHCCCEEEEHHHHHCCCEECCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEECCHHHHHE
YEKSGALLGLERVRAMDLTEGHIFVSKSQIFSEISHLFQMISEVLDFFKVQIFYISFSKR
ECCCCCEEEHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEECCC
DPKNKDKFFQDDQMWDQAEADLKQFLEQNKINYIEKIGEAAFYGPKIDFQVKTALNKELT
CCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCEEEEECCCCCEE
ISTLQLDFLLPKKFKISFINEHNKKETPILIHRGLIGTYERFIALLLEQSKGNLPFISPK
EEEEEEEEECCCCEEEEEECCCCCCCCCEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCC
QIIVIPVAEKHNDFAQKIHKILFDLGFNSEIDLRSERISRKIREANLEKINFQVIIGDEE
CEEEEEECCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCEEEEEEEEECCCC
ITKNEITYRKFGSPELFRISLVDFVDLLKSSEKNV
CCCCCCEEEECCCCCEEEEHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11353084 [H]