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The map label for this gene is greA

Identifier: 54020301

GI number: 54020301

Start: 878976

End: 879458

Strand: Direct

Name: greA

Synonym: mhp690

Alternate gene names: 54020301

Gene position: 878976-879458 (Clockwise)

Preceding gene: 54020300

Following gene: 54020302

Centisome position: 98.46

GC content: 35.2

Gene sequence:

>483_bases
ATGAAAAACACAGTTAATGACAAAATCCTCTTGACCCAGCAAAAACTTGAGGAAATTGAAAAAGAGCTTGAACATTTAAT
CAATGTCGAGCGCGTGAATGTAATCCAAGAAATCAAAGATGCCCGTTCACAAGGTGATCTTTCAGAAAATGCCGAATATG
ATGTTGCTCGCGAAAAACAGGGTATAATTGAATCGCGAATCCGTGAATTGGAAACAATTATTTCCAAAGCAAAAATAATT
AAGGCCGATTTAGGGTCAAGCAGAGTTTCAATTGGCTCAAAAGTTAGCCTTGAAAATGTTGAAAGTGGCGAGATTCAAAC
TTTCCAAATTGTCAGTTCAATAGATGCTGATCCTTTTAAGAGCAAAATTTCTAATTTTTCACCGATTGCACAGGCTCTTT
TAGGACAACACCAAGGTGATGAAGTTGAAGTTGATGTAAATGAAAAATATTCTGTAAGAATTTTGGAAGTAATTAACGAA
TAA

Upstream 100 bases:

>100_bases
GCAAAAAATCGTTTTTGATGAATATTTTTACTACGATTTTTTGCTAATTAAGAAAAAATAAATTAAAATTTAATGACTAC
CACCAAAAAAGGAGTATAAT

Downstream 100 bases:

>100_bases
TGCGTCTTTCAGTCCGTCAATTTTTCCCTGATTCGGCTGAAAAATGAAAAAAATTTGTTAAAATTGTAATTCCAGTTATA
TTTGCTACACTTTTTATCTC

Product: transcription elongation factor GreA

Products: NA

Alternate protein names: Transcript cleavage factor greA

Number of amino acids: Translated: 160; Mature: 160

Protein sequence:

>160_residues
MKNTVNDKILLTQQKLEEIEKELEHLINVERVNVIQEIKDARSQGDLSENAEYDVAREKQGIIESRIRELETIISKAKII
KADLGSSRVSIGSKVSLENVESGEIQTFQIVSSIDADPFKSKISNFSPIAQALLGQHQGDEVEVDVNEKYSVRILEVINE

Sequences:

>Translated_160_residues
MKNTVNDKILLTQQKLEEIEKELEHLINVERVNVIQEIKDARSQGDLSENAEYDVAREKQGIIESRIRELETIISKAKII
KADLGSSRVSIGSKVSLENVESGEIQTFQIVSSIDADPFKSKISNFSPIAQALLGQHQGDEVEVDVNEKYSVRILEVINE
>Mature_160_residues
MKNTVNDKILLTQQKLEEIEKELEHLINVERVNVIQEIKDARSQGDLSENAEYDVAREKQGIIESRIRELETIISKAKII
KADLGSSRVSIGSKVSLENVESGEIQTFQIVSSIDADPFKSKISNFSPIAQALLGQHQGDEVEVDVNEKYSVRILEVINE

Specific function: Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary

COG id: COG0782

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the greA/greB family

Homologues:

Organism=Escherichia coli, GI87082225, Length=136, Percent_Identity=40.4411764705882, Blast_Score=94, Evalue=6e-21,
Organism=Escherichia coli, GI87082261, Length=132, Percent_Identity=33.3333333333333, Blast_Score=63, Evalue=8e-12,

Paralogues:

None

Copy number: 259 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): GREA_MYCH2 (Q5ZZL8)

Other databases:

- EMBL:   AE017332
- RefSeq:   YP_116197.1
- HSSP:   P0A6W5
- ProteinModelPortal:   Q5ZZL8
- SMR:   Q5ZZL8
- STRING:   Q5ZZL8
- GeneID:   3105443
- GenomeReviews:   AE017332_GR
- KEGG:   mhy:mhp690
- eggNOG:   COG0782
- HOGENOM:   HBG753387
- ProtClustDB:   PRK00226
- BioCyc:   MHYO295358:MHP690-MONOMER
- GO:   GO:0006350
- HAMAP:   MF_00105
- InterPro:   IPR018151
- InterPro:   IPR006359
- InterPro:   IPR001437
- InterPro:   IPR022691
- Gene3D:   G3DSA:1.10.287.180
- Gene3D:   G3DSA:3.10.50.30
- TIGRFAMs:   TIGR01462

Pfam domain/function: PF01272 GreA_GreB; PF03449 GreA_GreB_N; SSF46557 GreA_GreB

EC number: NA

Molecular weight: Translated: 17976; Mature: 17976

Theoretical pI: Translated: 4.49; Mature: 4.49

Prosite motif: PS00829 GREAB_1; PS00830 GREAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
0.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
0.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNTVNDKILLTQQKLEEIEKELEHLINVERVNVIQEIKDARSQGDLSENAEYDVAREKQ
CCCCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHH
GIIESRIRELETIISKAKIIKADLGSSRVSIGSKVSLENVESGEIQTFQIVSSIDADPFK
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCHHH
SKISNFSPIAQALLGQHQGDEVEVDVNEKYSVRILEVINE
HHHCCCCHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHCC
>Mature Secondary Structure
MKNTVNDKILLTQQKLEEIEKELEHLINVERVNVIQEIKDARSQGDLSENAEYDVAREKQ
CCCCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHH
GIIESRIRELETIISKAKIIKADLGSSRVSIGSKVSLENVESGEIQTFQIVSSIDADPFK
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCHHH
SKISNFSPIAQALLGQHQGDEVEVDVNEKYSVRILEVINE
HHHCCCCHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA