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The map label for this gene is tsf

Identifier: 54020009

GI number: 54020009

Start: 68596

End: 69507

Strand: Direct

Name: tsf

Synonym: mhp059

Alternate gene names: 54020009

Gene position: 68596-69507 (Clockwise)

Preceding gene: 54020008

Following gene: 54020010

Centisome position: 7.68

GC content: 31.58

Gene sequence:

>912_bases
ATGTCACAAATTGATAAAATGGCAAAAATCAAAAAATTAAGGGAAATTAGTGATGCTCCCTTTGTTGATTGTAAAAAAGC
ATTAGAAAATTCCGATTATGATATTGATTTAGCAATTAATTGACTTAATAAAAACGGAAAATCAAAAGCTTTGAAAAAAT
CAGATCGAATTGCCGCTGAAGGGTTGGTTTTAGCAAAAAAAGATGCTAATTCCGTTTTAGTTTTTGAGCTAAATTCGGAA
ACTGATTTTGTTGCGAAAAATCAAAATTTTATAAATTTGCAGCAAAAGATTGGTGAATTACTACTTGCAAATGATTTTGT
AAATTTAGAAGACGCCTTACTTATTCAGGATGAGGCAGGTAGGTCAATTTCAGAATTAATGATTTTAGCAACAGCAACAA
TAGGCGAAAAAATTACCTTGCGTCGCGTTTTTAAAACAAAATATTCTTTAGAACAAAGTGTTGAAGTTTATACCCACTCA
AATGGTCAAATTGCAGTAATTACGATTTTAAAAGGCGGAAATTTAGAAATAGCAAAAAATATTTCCATGCATGTAGCGGC
ATTAAATCCCCAATATATTTTAAAAGTTGAAGTTCCAAACGAGAAATTGCAGGAAATTCAATTAGAAGTTGAGAAAAAGG
CTTTTGCTGAAGTTAAAAATTTTGAGAAAAAACCAGAAAATGTTAGGGTCGGCATTTTAAAAGGAATGATAGACAAGCAA
CTTTCAGAATTTGTTTTAGAACTTCAGCCTTTAGCGACTGACAGTGCTGTTACAGTCGAAAAATATTTAGCTCAAAATTC
AGCAACATTAGAAAAAGTAGTTCGTTTTGAGGTAGGCGAGGGGATCCAAAAGCAAAATGTTGATTTTTCCGCTGAGGTTA
ATCAGCAAATTCAGGAATTTCAGAAAAAATAG

Upstream 100 bases:

>100_bases
TGAAAAATTTGCTCGCCAAAAAAATACTTTAAAAGATAGTCAAGTTTTAAAACCAAACACAGAAATTCAAGCCTAAAAAA
TAAAAATACGAGAGGTTAAG

Downstream 100 bases:

>100_bases
AAAAATTATCAAACTAAAAATCTGAAGAGGGGTATAAAATTTTTCCTTTAAAAATAGGAAAAATTTTGTATTTTTTATAA
ACAAATGCTAGAATTTTTAA

Product: elongation factor Ts

Products: NA

Alternate protein names: EF-Ts [H]

Number of amino acids: Translated: 303; Mature: 302

Protein sequence:

>303_residues
MSQIDKMAKIKKLREISDAPFVDCKKALENSDYDIDLAINWLNKNGKSKALKKSDRIAAEGLVLAKKDANSVLVFELNSE
TDFVAKNQNFINLQQKIGELLLANDFVNLEDALLIQDEAGRSISELMILATATIGEKITLRRVFKTKYSLEQSVEVYTHS
NGQIAVITILKGGNLEIAKNISMHVAALNPQYILKVEVPNEKLQEIQLEVEKKAFAEVKNFEKKPENVRVGILKGMIDKQ
LSEFVLELQPLATDSAVTVEKYLAQNSATLEKVVRFEVGEGIQKQNVDFSAEVNQQIQEFQKK

Sequences:

>Translated_303_residues
MSQIDKMAKIKKLREISDAPFVDCKKALENSDYDIDLAIN*LNKNGKSKALKKSDRIAAEGLVLAKKDANSVLVFELNSE
TDFVAKNQNFINLQQKIGELLLANDFVNLEDALLIQDEAGRSISELMILATATIGEKITLRRVFKTKYSLEQSVEVYTHS
NGQIAVITILKGGNLEIAKNISMHVAALNPQYILKVEVPNEKLQEIQLEVEKKAFAEVKNFEKKPENVRVGILKGMIDKQ
LSEFVLELQPLATDSAVTVEKYLAQNSATLEKVVRFEVGEGIQKQNVDFSAEVNQQIQEFQKK
>Mature_302_residues
SQIDKMAKIKKLREISDAPFVDCKKALENSDYDIDLAIN*LNKNGKSKALKKSDRIAAEGLVLAKKDANSVLVFELNSET
DFVAKNQNFINLQQKIGELLLANDFVNLEDALLIQDEAGRSISELMILATATIGEKITLRRVFKTKYSLEQSVEVYTHSN
GQIAVITILKGGNLEIAKNISMHVAALNPQYILKVEVPNEKLQEIQLEVEKKAFAEVKNFEKKPENVRVGILKGMIDKQL
SEFVLELQPLATDSAVTVEKYLAQNSATLEKVVRFEVGEGIQKQNVDFSAEVNQQIQEFQKK

Specific function: Associates with the EF-Tu.GDP complex and induces the exchange of GDP to GTP. It remains bound to the aminoacyl-tRNA.EF- Tu.GTP complex up to the GTP hydrolysis stage on the ribosome [H]

COG id: COG0264

COG function: function code J; Translation elongation factor Ts

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the EF-Ts family [H]

Homologues:

Organism=Escherichia coli, GI1786366, Length=287, Percent_Identity=34.8432055749129, Blast_Score=149, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI17561440, Length=298, Percent_Identity=25.8389261744966, Blast_Score=64, Evalue=8e-11,
Organism=Drosophila melanogaster, GI19921466, Length=163, Percent_Identity=31.9018404907975, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: 2670 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1100 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 4173 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 14563 Molecules/Cell In: Growth Phase, Gluco

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001816
- InterPro:   IPR014039
- InterPro:   IPR018101
- InterPro:   IPR009060
- InterPro:   IPR000449 [H]

Pfam domain/function: PF00889 EF_TS; PF00627 UBA [H]

EC number: NA

Molecular weight: Translated: 33806; Mature: 33675

Theoretical pI: Translated: 5.59; Mature: 5.59

Prosite motif: PS01127 EF_TS_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQIDKMAKIKKLREISDAPFVDCKKALENSDYDIDLAINLNKNGKSKALKKSDRIAAEG
CCHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCEEEEEEEECCCCCHHHHHHHCCHHHCC
LVLAKKDANSVLVFELNSETDFVAKNQNFINLQQKIGELLLANDFVNLEDALLIQDEAGR
EEEEECCCCEEEEEEECCCCCEEECCCCEEEHHHHHHHHHHHCCCCCCCCEEEEECCCCC
SISELMILATATIGEKITLRRVFKTKYSLEQSVEVYTHSNGQIAVITILKGGNLEIAKNI
CHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCEEEEEECCCCEEEEEEEECCCEEEEECC
SMHVAALNPQYILKVEVPNEKLQEIQLEVEKKAFAEVKNFEKKPENVRVGILKGMIDKQL
EEEEEEECCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHH
SEFVLELQPLATDSAVTVEKYLAQNSATLEKVVRFEVGEGIQKQNVDFSAEVNQQIQEFQ
HHHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHCCCCHHCCCCCHHHHHHHHHHHH
KK
CC
>Mature Secondary Structure 
SQIDKMAKIKKLREISDAPFVDCKKALENSDYDIDLAINLNKNGKSKALKKSDRIAAEG
CHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCEEEEEEEECCCCCHHHHHHHCCHHHCC
LVLAKKDANSVLVFELNSETDFVAKNQNFINLQQKIGELLLANDFVNLEDALLIQDEAGR
EEEEECCCCEEEEEEECCCCCEEECCCCEEEHHHHHHHHHHHCCCCCCCCEEEEECCCCC
SISELMILATATIGEKITLRRVFKTKYSLEQSVEVYTHSNGQIAVITILKGGNLEIAKNI
CHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCEEEEEECCCCEEEEEEEECCCEEEEECC
SMHVAALNPQYILKVEVPNEKLQEIQLEVEKKAFAEVKNFEKKPENVRVGILKGMIDKQL
EEEEEEECCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHH
SEFVLELQPLATDSAVTVEKYLAQNSATLEKVVRFEVGEGIQKQNVDFSAEVNQQIQEFQ
HHHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHCCCCHHCCCCCHHHHHHHHHHHH
KK
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA