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The map label for this gene is nifA [H]

Identifier: 53723148

GI number: 53723148

Start: 2887571

End: 2889091

Strand: Reverse

Name: nifA [H]

Synonym: BPSS2132

Alternate gene names: 53723148

Gene position: 2889091-2887571 (Counterclockwise)

Preceding gene: 53723152

Following gene: 53723147

Centisome position: 91.05

GC content: 69.69

Gene sequence:

>1521_bases
GTGGAGACGAATGCACCGATTTCGGGCGGCTGGGTTCGGCTGCCCGCCGATTACGGCGACGTGCTGCGGCGCGCGGCGGA
GTCGCTGTTCAAGACCTTCGAGCACTCGAGCGTCGGCACGCTGATCGTCGACAAGGATGCGCGCGTCGTCTGGATCAATC
AGCGTTACGCGGCGCGTTTCGGGTTTGCCGATCCGCAGCAGGCGATCGGCCGCGATTGCGAAGCGGTGATTCCGCACAGC
CTGATGCGCGAGGTGGTCGCGACCGGCCGCCCGATCCTGCTCGACATCATGGAGACGGGCCGTGAGCCGCTCGTCGTCAC
GCGCCTGCCGCTGACGGACGACGCGGGCGAGACCGTCGGCGCGATCGGCTTCGCGCTGTTCGACGAGCTGAAGACGCTCA
CGCCGCTCTTTTCGCGCTACATGCAGGTCCAGCAGGAGCTGATCGCGACGCAACGCTCGCTCGCGCAGGCGCGGCGGGCG
AAATACACGTTCGCGAGCTTCGTCGGCACGAGCGCGGTGAGCCTCGAGACGAAGCGGCAGGGGCGGCGCGCCGCGCAGGT
CGATTCGCCGGTGCTGCTGCTCGGCGAGACGGGCACCGGTAAGGAGCTGCTCGCGCATGCGATCCACGCGGCGTCCGCGC
GGGCATTGAAGCCGCTCGTGACCGTCAACGTCGCGGCGATTCCCGATGCGCTGCTCGAAACCGAGTTCTTCGGCGCGGCG
CCGGGCGCGTACACGGGCGCGGATCGCAAGGGGCGCGTCGGCAAGTTCGAGCTTGCCGACGGCGGCACGCTCTTTCTCGA
CGAAATCGGCGACATGCCGGTGCCGCTGCAGGGCAAGCTGCTGCGCGTGCTGCAGGACAAGGAGTTCGAGCCGGTCGGCT
CGAACCGGATCGTGCGCGCGAATGTGCGGATCATCGCGGCGACGTCGGCCGAATTGCCGGCGCTCGTCGCGGAAGGGCGC
TTTCGCGCGGACCTTTATTACCGGCTGAACGTGCTGACGATCCATGCGCCGCCGCTGCGCGAGCGCGCATCGGACATCGA
GGCGCTCGTCTACACGATGCTCGAGGAACTCGCCGCGCAGCATGGGCTGGCCGAGCACTGCGAACTGACCGACGACGCGC
TGCGCCTGCTGTGCGCGTATCCGTGGCCCGGTAACGTGCGCGAACTGCGCAACACGCTCGAGCGCGCGCTGATGCTGTCC
GATCGCGCGTTGATCGATGCGCGCGCGCTCGCGCCGTTCATCGGGCCGGCGCGCGGCGCGGGGGGCGGTGTCGGGGCGGG
CGGTGGGGCGGGGGCGGGCGGCGTCGGTCCGGCTGCGGTCGCCGTCGCGGCGCAGACTGCCATGGCCGATACGCGCGCGG
CGGCGTCATCCTATGCGGACGCATTCGCCGCGTGGGAGCGTCAATTCCTGATCGACGCGCTTGCCGCGTCCAACGGCAAG
GTGACGGAAGCGGCCGCGCGCATCGGCATCGGGCGTGCGACGTTCTACAAGAAGCTCGCGACGCTCGGCATCGATACGTA
G

Upstream 100 bases:

>100_bases
GCGATGCTCGGCGATTCATGGCCGGGGCTTTTATGACGGGCATTTGCCGAATGTCTCTTGCATGAGATAATTCGTCTCGA
TTTGTGGACAAGAAAGAAAA

Downstream 100 bases:

>100_bases
CGGGCGGCTCGCGCGACGCGCGGGATGCGCTTGCGTTCGCATCGATCCCATCGATCGCGACAATCGCATCGATGCTCGCG
CATGCGCGGGCGCATTCGCA

Product: sigma-54 dependent transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 506; Mature: 506

Protein sequence:

>506_residues
METNAPISGGWVRLPADYGDVLRRAAESLFKTFEHSSVGTLIVDKDARVVWINQRYAARFGFADPQQAIGRDCEAVIPHS
LMREVVATGRPILLDIMETGREPLVVTRLPLTDDAGETVGAIGFALFDELKTLTPLFSRYMQVQQELIATQRSLAQARRA
KYTFASFVGTSAVSLETKRQGRRAAQVDSPVLLLGETGTGKELLAHAIHAASARALKPLVTVNVAAIPDALLETEFFGAA
PGAYTGADRKGRVGKFELADGGTLFLDEIGDMPVPLQGKLLRVLQDKEFEPVGSNRIVRANVRIIAATSAELPALVAEGR
FRADLYYRLNVLTIHAPPLRERASDIEALVYTMLEELAAQHGLAEHCELTDDALRLLCAYPWPGNVRELRNTLERALMLS
DRALIDARALAPFIGPARGAGGGVGAGGGAGAGGVGPAAVAVAAQTAMADTRAAASSYADAFAAWERQFLIDALAASNGK
VTEAAARIGIGRATFYKKLATLGIDT

Sequences:

>Translated_506_residues
METNAPISGGWVRLPADYGDVLRRAAESLFKTFEHSSVGTLIVDKDARVVWINQRYAARFGFADPQQAIGRDCEAVIPHS
LMREVVATGRPILLDIMETGREPLVVTRLPLTDDAGETVGAIGFALFDELKTLTPLFSRYMQVQQELIATQRSLAQARRA
KYTFASFVGTSAVSLETKRQGRRAAQVDSPVLLLGETGTGKELLAHAIHAASARALKPLVTVNVAAIPDALLETEFFGAA
PGAYTGADRKGRVGKFELADGGTLFLDEIGDMPVPLQGKLLRVLQDKEFEPVGSNRIVRANVRIIAATSAELPALVAEGR
FRADLYYRLNVLTIHAPPLRERASDIEALVYTMLEELAAQHGLAEHCELTDDALRLLCAYPWPGNVRELRNTLERALMLS
DRALIDARALAPFIGPARGAGGGVGAGGGAGAGGVGPAAVAVAAQTAMADTRAAASSYADAFAAWERQFLIDALAASNGK
VTEAAARIGIGRATFYKKLATLGIDT
>Mature_506_residues
METNAPISGGWVRLPADYGDVLRRAAESLFKTFEHSSVGTLIVDKDARVVWINQRYAARFGFADPQQAIGRDCEAVIPHS
LMREVVATGRPILLDIMETGREPLVVTRLPLTDDAGETVGAIGFALFDELKTLTPLFSRYMQVQQELIATQRSLAQARRA
KYTFASFVGTSAVSLETKRQGRRAAQVDSPVLLLGETGTGKELLAHAIHAASARALKPLVTVNVAAIPDALLETEFFGAA
PGAYTGADRKGRVGKFELADGGTLFLDEIGDMPVPLQGKLLRVLQDKEFEPVGSNRIVRANVRIIAATSAELPALVAEGR
FRADLYYRLNVLTIHAPPLRERASDIEALVYTMLEELAAQHGLAEHCELTDDALRLLCAYPWPGNVRELRNTLERALMLS
DRALIDARALAPFIGPARGAGGGVGAGGGAGAGGVGPAAVAVAAQTAMADTRAAASSYADAFAAWERQFLIDALAASNGK
VTEAAARIGIGRATFYKKLATLGIDT

Specific function: Required for activation of most nif operons, which are directly involved in nitrogen fixation [H]

COG id: COG3829

COG function: function code KT; Transcriptional regulator containing PAS, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1789087, Length=353, Percent_Identity=41.3597733711048, Blast_Score=241, Evalue=9e-65,
Organism=Escherichia coli, GI1788550, Length=390, Percent_Identity=36.6666666666667, Blast_Score=234, Evalue=1e-62,
Organism=Escherichia coli, GI87082117, Length=366, Percent_Identity=38.7978142076503, Blast_Score=230, Evalue=1e-61,
Organism=Escherichia coli, GI1788905, Length=340, Percent_Identity=40.2941176470588, Blast_Score=230, Evalue=1e-61,
Organism=Escherichia coli, GI1789233, Length=335, Percent_Identity=38.5074626865672, Blast_Score=223, Evalue=2e-59,
Organism=Escherichia coli, GI1790437, Length=313, Percent_Identity=42.8115015974441, Blast_Score=221, Evalue=8e-59,
Organism=Escherichia coli, GI1790299, Length=320, Percent_Identity=42.5, Blast_Score=206, Evalue=3e-54,
Organism=Escherichia coli, GI87082152, Length=252, Percent_Identity=45.6349206349206, Blast_Score=203, Evalue=2e-53,
Organism=Escherichia coli, GI1786524, Length=264, Percent_Identity=45.0757575757576, Blast_Score=200, Evalue=2e-52,
Organism=Escherichia coli, GI87081872, Length=232, Percent_Identity=43.9655172413793, Blast_Score=191, Evalue=1e-49,
Organism=Escherichia coli, GI1787583, Length=238, Percent_Identity=43.2773109243698, Blast_Score=187, Evalue=9e-49,
Organism=Escherichia coli, GI87081858, Length=351, Percent_Identity=33.6182336182336, Blast_Score=170, Evalue=2e-43,
Organism=Escherichia coli, GI1789828, Length=221, Percent_Identity=38.0090497737557, Blast_Score=126, Evalue=3e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR003018
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR010113
- InterPro:   IPR002078 [H]

Pfam domain/function: PF01590 GAF; PF02954 HTH_8; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 53999; Mature: 53999

Theoretical pI: Translated: 6.65; Mature: 6.65

Prosite motif: PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METNAPISGGWVRLPADYGDVLRRAAESLFKTFEHSSVGTLIVDKDARVVWINQRYAARF
CCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCEEEEEECHHHHHC
GFADPQQAIGRDCEAVIPHSLMREVVATGRPILLDIMETGREPLVVTRLPLTDDAGETVG
CCCCHHHHHCCCCHHHCCHHHHHHHHHCCCCEEEEHHHCCCCCEEEEECCCCCCCCCHHH
AIGFALFDELKTLTPLFSRYMQVQQELIATQRSLAQARRAKYTFASFVGTSAVSLETKRQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHC
GRRAAQVDSPVLLLGETGTGKELLAHAIHAASARALKPLVTVNVAAIPDALLETEFFGAA
CCHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCHHHEEEHHHCCHHHHHHHHCCCC
PGAYTGADRKGRVGKFELADGGTLFLDEIGDMPVPLQGKLLRVLQDKEFEPVGSNRIVRA
CCCCCCCCCCCCCCEEEECCCCEEEEECCCCCCCCCHHHHHHHHHCCCCCCCCCCCEEEE
NVRIIAATSAELPALVAEGRFRADLYYRLNVLTIHAPPLRERASDIEALVYTMLEELAAQ
EEEEEEECCCCCCHHHHCCCEEEEEEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHH
HGLAEHCELTDDALRLLCAYPWPGNVRELRNTLERALMLSDRALIDARALAPFIGPARGA
CCCHHHCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCC
GGGVGAGGGAGAGGVGPAAVAVAAQTAMADTRAAASSYADAFAAWERQFLIDALAASNGK
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
VTEAAARIGIGRATFYKKLATLGIDT
HHHHHHHHCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure
METNAPISGGWVRLPADYGDVLRRAAESLFKTFEHSSVGTLIVDKDARVVWINQRYAARF
CCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCEEEEEECHHHHHC
GFADPQQAIGRDCEAVIPHSLMREVVATGRPILLDIMETGREPLVVTRLPLTDDAGETVG
CCCCHHHHHCCCCHHHCCHHHHHHHHHCCCCEEEEHHHCCCCCEEEEECCCCCCCCCHHH
AIGFALFDELKTLTPLFSRYMQVQQELIATQRSLAQARRAKYTFASFVGTSAVSLETKRQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHC
GRRAAQVDSPVLLLGETGTGKELLAHAIHAASARALKPLVTVNVAAIPDALLETEFFGAA
CCHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCHHHEEEHHHCCHHHHHHHHCCCC
PGAYTGADRKGRVGKFELADGGTLFLDEIGDMPVPLQGKLLRVLQDKEFEPVGSNRIVRA
CCCCCCCCCCCCCCEEEECCCCEEEEECCCCCCCCCHHHHHHHHHCCCCCCCCCCCEEEE
NVRIIAATSAELPALVAEGRFRADLYYRLNVLTIHAPPLRERASDIEALVYTMLEELAAQ
EEEEEEECCCCCCHHHHCCCEEEEEEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHH
HGLAEHCELTDDALRLLCAYPWPGNVRELRNTLERALMLSDRALIDARALAPFIGPARGA
CCCHHHCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCC
GGGVGAGGGAGAGGVGPAAVAVAAQTAMADTRAAASSYADAFAAWERQFLIDALAASNGK
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
VTEAAARIGIGRATFYKKLATLGIDT
HHHHHHHHCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2840552; 2450865 [H]