Click here to switch to the map view.

The map label for this gene is ilvE

Identifier: 53722829

GI number: 53722829

Start: 2468994

End: 2469938

Strand: Reverse

Name: ilvE

Synonym: BPSS1810

Alternate gene names: 53722829

Gene position: 2469938-2468994 (Counterclockwise)

Preceding gene: 53722830

Following gene: 53722828

Centisome position: 77.84

GC content: 65.5

Gene sequence:

>945_bases
ATGAAATCGAGCTACGTGTGGAAGAACGGTGAACTGGTGCCGTGGGAGCATGCGCAGGTGCATGTGATGAGCCATGCGCT
GCATTACGGGACGAGTGTGTTCGAAGGCGTGCGCGCGTACGAGATCGGCGAGAAGGCGGCGATCCTCTGCGGCCGTGAGC
ACTTCGAGCGCCTGCTGTTCAGCTGCAAGGTCGCGCGGATTGCGTCGCCGATGAGCGTCGGGCAATGGATGGAGGCGACG
GTCGAGACGCTGCGCGCGAACGGTCAGCGCAGCGCGTACATCCGGCCGCTCGTCTATCGCGGCGCGGGCGAATCGCTCGG
GCTCGACGCGCGACAGTGCCCGGCCGAGGCGCTGCTCATCACCACACCGTGGGGCGCGTATCTCGGCGACGAGGCGTTGC
AGCAAGGCGTCGACGTGCAGGTGAGCAGCTGGCGGCGCAACGGCGCGGGAGCGGCAAGCACGCTGGCGAAGATCGGCGGC
CAGTACGTCAACGGTCAGGCGATCGTGATGGAGGCGCACGAGAACGGGATGAGCGAAGGCATCGCGCTCGATACGAACGG
TTTCGTCAGCGAAGGCAGCGGCGAGAACGTGTTCCTTGTCTACAAGAACGAAATCTTCACGCCGTCGATCGGCAGCAGCA
TCCTGAGCGGCATCACGCGCCACTGCGTGATCCGGATCGCGCGCGATCTCGGCTACACCGTGACCGAAACGAGCATTCCG
CGCGAAATGCTCTACCTCGCCGACGAAATCTTCTTCACGGGCACCGCCGTCGAGATTTGTCCCGTTCGCTCGGTCGACCG
GATGCCGGTCGGCAGCGGCAAGCGCGGCGCGGTGACGAAGGCGATCCAGGATCAGTACTTCGGCATCGTGCGCGGCACGC
ACGCGGACAAATGGAACTGGCTCACGCCCGTGGCGGTGCCGTCGCGCGACGGAGCACGGGCATGA

Upstream 100 bases:

>100_bases
AGAACCGGCTCGAGCGCAAGGTACGCGGCGCGATCGCAGAGGGGCGCAGCGTCGTCGAGGCATTCAATCAATACAACGTC
ATGTGAGGAGCGCAACAACG

Downstream 100 bases:

>100_bases
GCGCGCGCGAAGCCGCAGCCGAATCGCCTTGGCTGATCGTCCATGAGGCGCGCAACGCGCGCGTGCGGCTCTTCTGCTTT
CACTACGCGGGCGCGACCGC

Product: branched-chain amino acid aminotransferase

Products: NA

Alternate protein names: BCAT; Transaminase B [H]

Number of amino acids: Translated: 314; Mature: 314

Protein sequence:

>314_residues
MKSSYVWKNGELVPWEHAQVHVMSHALHYGTSVFEGVRAYEIGEKAAILCGREHFERLLFSCKVARIASPMSVGQWMEAT
VETLRANGQRSAYIRPLVYRGAGESLGLDARQCPAEALLITTPWGAYLGDEALQQGVDVQVSSWRRNGAGAASTLAKIGG
QYVNGQAIVMEAHENGMSEGIALDTNGFVSEGSGENVFLVYKNEIFTPSIGSSILSGITRHCVIRIARDLGYTVTETSIP
REMLYLADEIFFTGTAVEICPVRSVDRMPVGSGKRGAVTKAIQDQYFGIVRGTHADKWNWLTPVAVPSRDGARA

Sequences:

>Translated_314_residues
MKSSYVWKNGELVPWEHAQVHVMSHALHYGTSVFEGVRAYEIGEKAAILCGREHFERLLFSCKVARIASPMSVGQWMEAT
VETLRANGQRSAYIRPLVYRGAGESLGLDARQCPAEALLITTPWGAYLGDEALQQGVDVQVSSWRRNGAGAASTLAKIGG
QYVNGQAIVMEAHENGMSEGIALDTNGFVSEGSGENVFLVYKNEIFTPSIGSSILSGITRHCVIRIARDLGYTVTETSIP
REMLYLADEIFFTGTAVEICPVRSVDRMPVGSGKRGAVTKAIQDQYFGIVRGTHADKWNWLTPVAVPSRDGARA
>Mature_314_residues
MKSSYVWKNGELVPWEHAQVHVMSHALHYGTSVFEGVRAYEIGEKAAILCGREHFERLLFSCKVARIASPMSVGQWMEAT
VETLRANGQRSAYIRPLVYRGAGESLGLDARQCPAEALLITTPWGAYLGDEALQQGVDVQVSSWRRNGAGAASTLAKIGG
QYVNGQAIVMEAHENGMSEGIALDTNGFVSEGSGENVFLVYKNEIFTPSIGSSILSGITRHCVIRIARDLGYTVTETSIP
REMLYLADEIFFTGTAVEICPVRSVDRMPVGSGKRGAVTKAIQDQYFGIVRGTHADKWNWLTPVAVPSRDGARA

Specific function: Acts on leucine, isoleucine and valine [H]

COG id: COG0115

COG function: function code EH; Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Escherichia coli, GI48994963, Length=307, Percent_Identity=49.185667752443, Blast_Score=294, Evalue=4e-81,
Organism=Saccharomyces cerevisiae, GI6322002, Length=337, Percent_Identity=25.5192878338279, Blast_Score=69, Evalue=1e-12,

Paralogues:

None

Copy number: 2342 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 11,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001544
- InterPro:   IPR018300
- InterPro:   IPR005785 [H]

Pfam domain/function: PF01063 Aminotran_4 [H]

EC number: =2.6.1.42 [H]

Molecular weight: Translated: 34214; Mature: 34214

Theoretical pI: Translated: 6.94; Mature: 6.94

Prosite motif: PS00770 AA_TRANSFER_CLASS_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSSYVWKNGELVPWEHAQVHVMSHALHYGTSVFEGVRAYEIGEKAAILCGREHFERLLF
CCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCHHHHHCCCHHEEEECHHHHHHHHH
SCKVARIASPMSVGQWMEATVETLRANGQRSAYIRPLVYRGAGESLGLDARQCPAEALLI
HHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCEEECEEEEECCCCCCCCCHHHCCCCEEEE
TTPWGAYLGDEALQQGVDVQVSSWRRNGAGAASTLAKIGGQYVNGQAIVMEAHENGMSEG
ECCCCHHCCHHHHHCCCCEEEECCCCCCCCHHHHHHHHCCEEECCEEEEEEECCCCCCCC
IALDTNGFVSEGSGENVFLVYKNEIFTPSIGSSILSGITRHCVIRIARDLGYTVTETSIP
EEECCCCCEECCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEECCCCC
REMLYLADEIFFTGTAVEICPVRSVDRMPVGSGKRGAVTKAIQDQYFGIVRGTHADKWNW
HHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHEEECCCCCCCCC
LTPVAVPSRDGARA
CCCEECCCCCCCCC
>Mature Secondary Structure
MKSSYVWKNGELVPWEHAQVHVMSHALHYGTSVFEGVRAYEIGEKAAILCGREHFERLLF
CCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCHHHHHCCCHHEEEECHHHHHHHHH
SCKVARIASPMSVGQWMEATVETLRANGQRSAYIRPLVYRGAGESLGLDARQCPAEALLI
HHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCEEECEEEEECCCCCCCCCHHHCCCCEEEE
TTPWGAYLGDEALQQGVDVQVSSWRRNGAGAASTLAKIGGQYVNGQAIVMEAHENGMSEG
ECCCCHHCCHHHHHCCCCEEEECCCCCCCCHHHHHHHHCCEEECCEEEEEEECCCCCCCC
IALDTNGFVSEGSGENVFLVYKNEIFTPSIGSSILSGITRHCVIRIARDLGYTVTETSIP
EEECCCCCEECCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEECCCCC
REMLYLADEIFFTGTAVEICPVRSVDRMPVGSGKRGAVTKAIQDQYFGIVRGTHADKWNW
HHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHEEECCCCCCCCC
LTPVAVPSRDGARA
CCCEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9371463 [H]