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The map label for this gene is ysgA [C]

Identifier: 53722788

GI number: 53722788

Start: 2421642

End: 2422517

Strand: Reverse

Name: ysgA [C]

Synonym: BPSS1767

Alternate gene names: 53722788

Gene position: 2422517-2421642 (Counterclockwise)

Preceding gene: 53722790

Following gene: 53722785

Centisome position: 76.35

GC content: 66.78

Gene sequence:

>876_bases
ATGTTGAAACCCGAAGTCGACAGTCTAGTTCCGCACGTTCCGTTCAGCCGCCGCAAGTTCGTCCAGGCGGCGCTCGGCGG
CACGTTCGCCGCGGCGGTGCTGCCCGTGTCCGCGCAGACGATCACCACCGATGCCGCCGGCCTAGACGTCGACACCGTCC
AGATCCGCTCGGGCGACGCGAGCGTGCCCGCCTATCGCGCGCAGCCGGACGGCAAGAGCAATCTGCCGGTGATCGTCGTG
ATCCACGAGGTGTTCGGCGTGCATGCGCACATCGCCGACATCTGCCGGCGCTTCGCGAAGCTCGGCTATCTGGCGATCGC
GCCGGATCTGTATGCGCGGCAGGGCGATCCGTCGAAGCATGCGTCGATCCAGGAACTGATCGATCAGGTGGTCAGCAAGG
TGCCCGACCGTCAGGTGATCGAGGATCTCGACGCGACGGTGCGATGGGCGGGCAAGAACGGCGGGGACCTGTCGCGGCTC
GGCGTGACGGGTTTTTGTTGGGGCGGCCGGCAGACGTGGCTTTTCGCCGAGCACAATCCGCACGTGCGCGCCGCTGTCGC
GTGGTACGGCAAGGTGGCCGGCGAGACGAACGAGATGACGCCGTTCAATCCTGACGATCATGCGGCGCAACTGAAGGCGC
CGACGCTCGGCCTCTACGGCGGCAAGGACGACAGCATTTCGCAGCGCTCGCTCGCGCGGATGCGCGAGCGTCTCGCGGCG
GCCGGCACGCAGGCCGCGCGCGAATCGGAAATCGTCGTGTATCCGGATGCGGGCCACGCGTTCTTCGCCGATTACCGCCC
GAGTTACGTGAAGGCCGATGCCGACGACGGCTGGAAGCGCGCTATCGCATGGTTTCGGCATCACGGCGTGATGTGA

Upstream 100 bases:

>100_bases
CGCGCGGCGCGACTGCATCGCGCCGGCCGCCGTGATTCGAATTCATGTCGGCGACGCGTGTGCCGCGCGTGCGCGACAGC
GAGAAGAGAGGAGCGCATCC

Downstream 100 bases:

>100_bases
TGCCGCGCGTGCGCCGCCACGGGTGGGCGGCGCACGCGGCAAGGCGCGGCTGGCGCGCTTACGGGTTCGCGCCCGTCGCG
ACGGGGCGGGACGGGTCCGC

Product: carboxymethylenebutenolidase

Products: NA

Alternate protein names: Dienelactone hydrolase; DLH [H]

Number of amino acids: Translated: 291; Mature: 291

Protein sequence:

>291_residues
MLKPEVDSLVPHVPFSRRKFVQAALGGTFAAAVLPVSAQTITTDAAGLDVDTVQIRSGDASVPAYRAQPDGKSNLPVIVV
IHEVFGVHAHIADICRRFAKLGYLAIAPDLYARQGDPSKHASIQELIDQVVSKVPDRQVIEDLDATVRWAGKNGGDLSRL
GVTGFCWGGRQTWLFAEHNPHVRAAVAWYGKVAGETNEMTPFNPDDHAAQLKAPTLGLYGGKDDSISQRSLARMRERLAA
AGTQAARESEIVVYPDAGHAFFADYRPSYVKADADDGWKRAIAWFRHHGVM

Sequences:

>Translated_291_residues
MLKPEVDSLVPHVPFSRRKFVQAALGGTFAAAVLPVSAQTITTDAAGLDVDTVQIRSGDASVPAYRAQPDGKSNLPVIVV
IHEVFGVHAHIADICRRFAKLGYLAIAPDLYARQGDPSKHASIQELIDQVVSKVPDRQVIEDLDATVRWAGKNGGDLSRL
GVTGFCWGGRQTWLFAEHNPHVRAAVAWYGKVAGETNEMTPFNPDDHAAQLKAPTLGLYGGKDDSISQRSLARMRERLAA
AGTQAARESEIVVYPDAGHAFFADYRPSYVKADADDGWKRAIAWFRHHGVM
>Mature_291_residues
MLKPEVDSLVPHVPFSRRKFVQAALGGTFAAAVLPVSAQTITTDAAGLDVDTVQIRSGDASVPAYRAQPDGKSNLPVIVV
IHEVFGVHAHIADICRRFAKLGYLAIAPDLYARQGDPSKHASIQELIDQVVSKVPDRQVIEDLDATVRWAGKNGGDLSRL
GVTGFCWGGRQTWLFAEHNPHVRAAVAWYGKVAGETNEMTPFNPDDHAAQLKAPTLGLYGGKDDSISQRSLARMRERLAA
AGTQAARESEIVVYPDAGHAFFADYRPSYVKADADDGWKRAIAWFRHHGVM

Specific function: Unknown

COG id: COG0412

COG function: function code Q; Dienelactone hydrolase and related enzymes

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dienelactone hydrolase family [H]

Homologues:

Organism=Escherichia coli, GI48994982, Length=262, Percent_Identity=52.6717557251908, Blast_Score=283, Evalue=8e-78,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002925
- InterPro:   IPR006311 [H]

Pfam domain/function: PF01738 DLH [H]

EC number: =3.1.1.45 [H]

Molecular weight: Translated: 31662; Mature: 31662

Theoretical pI: Translated: 7.40; Mature: 7.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKPEVDSLVPHVPFSRRKFVQAALGGTFAAAVLPVSAQTITTDAAGLDVDTVQIRSGDA
CCCCCHHHHCCCCCCHHHHHHHHHHCCHHHHHHEECCCCEEECCCCCCCEEEEEEECCCC
SVPAYRAQPDGKSNLPVIVVIHEVFGVHAHIADICRRFAKLGYLAIAPDLYARQGDPSKH
CCCCEECCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHCCEEEECCHHHHCCCCCHHH
ASIQELIDQVVSKVPDRQVIEDLDATVRWAGKNGGDLSRLGVTGFCWGGRQTWLFAEHNP
HHHHHHHHHHHHHCCCHHHHHHHCHHEEECCCCCCCCHHCCCEEEEECCCEEEEEECCCC
HVRAAVAWYGKVAGETNEMTPFNPDDHAAQLKAPTLGLYGGKDDSISQRSLARMRERLAA
CEEEEEEEHHHHCCCCCCCCCCCCCCCHHEECCCEEEEECCCCCCHHHHHHHHHHHHHHH
AGTQAARESEIVVYPDAGHAFFADYRPSYVKADADDGWKRAIAWFRHHGVM
HCCHHHCCCCEEEECCCCCEEEECCCCCEEECCCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure
MLKPEVDSLVPHVPFSRRKFVQAALGGTFAAAVLPVSAQTITTDAAGLDVDTVQIRSGDA
CCCCCHHHHCCCCCCHHHHHHHHHHCCHHHHHHEECCCCEEECCCCCCCEEEEEEECCCC
SVPAYRAQPDGKSNLPVIVVIHEVFGVHAHIADICRRFAKLGYLAIAPDLYARQGDPSKH
CCCCEECCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHCCEEEECCHHHHCCCCCHHH
ASIQELIDQVVSKVPDRQVIEDLDATVRWAGKNGGDLSRLGVTGFCWGGRQTWLFAEHNP
HHHHHHHHHHHHHCCCHHHHHHHCHHEEECCCCCCCCHHCCCEEEEECCCEEEEEECCCC
HVRAAVAWYGKVAGETNEMTPFNPDDHAAQLKAPTLGLYGGKDDSISQRSLARMRERLAA
CEEEEEEEHHHHCCCCCCCCCCCCCCCHHEECCCEEEEECCCCCCHHHHHHHHHHHHHHH
AGTQAARESEIVVYPDAGHAFFADYRPSYVKADADDGWKRAIAWFRHHGVM
HCCHHHCCCCEEEECCCCCEEEECCCCCEEECCCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9168622 [H]