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The map label for this gene is pilN

Identifier: 53722623

GI number: 53722623

Start: 2174832

End: 2176601

Strand: Reverse

Name: pilN

Synonym: BPSS1600

Alternate gene names: 53722623

Gene position: 2176601-2174832 (Counterclockwise)

Preceding gene: 53722624

Following gene: 53722622

Centisome position: 68.6

GC content: 71.53

Gene sequence:

>1770_bases
ATGCGCGTGCTGTTCGCCATTTCATTGCTGGCCGTCGCATGGCTCGCCGGCTGCACCGGCCTTCGCGGCGGCATCGAGCG
CGACGCGCGGCGCGATTCGAACGAAACCGGCGCGCTGCTCAAGCGCGCATCCGACGGCGACAACAGCGTGCGCTCGCTCG
CGCCCGTCGTCGTCGACAACGGGCTGTGGGTGTCCGCGGGCACCGTCAAGCTGCGCAACAGCGAGCAGTTGCCGCCGCTG
TTCGACGAGCCGGCGTCGTTCGATCGCTCGGTCGCTTCGTTGTCCGAATTCGCCGAGCAGATCACGCGCCTGACCCAGGT
GCCGACGCAGGTCGCCGCGAGCGCGCAGCAGGCGGCCGCGCGTTCGCAGCACGGCGGCGCGGGCGACGCCGCGCGCGGCG
CGCCGGCCTTTCTCGACGCATCGGGCGCGCGCGCGGTGCCGCCGCTGCCCCCCGGCCTGCCGGGCGGCGCATCGGCGGGC
GGCGGCAAGACGGGCGGCGGCGCGGCGGGCGCGGGCTCGGAGGGCGGCGGCACGTCGTTCGCGCCGGTGCGCGTGCTGTA
CGCGGGCGGCACGCTGCGCGGCCTGCTCGACGCGGCCTGCGCGCGCTTCGGCGTGTCCTGGAAGTACGAGCAGGGGGCGA
TCCGCTTCTTCTTCACCGATACCCGCACGTTCCAGGTCAACGCGATTCCGGGCGACTCGTCGCTGAACGCATCGGTCGTG
AGCGGCGCGACGAGCGACGGCGCCGCGGGCGGCTCGCAGTCGGGCGGCTCGGGGGGCTCGGGCGGCGGCATCAACGGCGC
GGGCTCCGGCACGACGGGCCTCACCGCGAACAACACGGCGAACACCGCGGTGAACTCGCAGCTGTCGGTGTTCAACGGCC
TGCAAAGCGCGATCCAATCGATGCTCTCGCGCTACGGCAGCTCGGTCGCCTCGCCCGCGACGGGATCGATCTCGGTGACC
GACACGCCCGACGTGCTCGAGCGCGTCGCCGCGTTCATGGCGCAGCAGAACCGCTCGCTGTCGCGGCAGGTGCTGCTCAA
CGTGACCGTGCTCAGCGTATCGCTGCAGGCGGGCGACGCGTACGGGATCGACTGGAACCTCGTCTACAAGACGATGTCCG
CGGGGTTCGGCATCACCAATCCGTTCCATCCGACGGCGCTGACGGCGCCCGCCGACATGTCCGCGGCGGTGCTCAGCCCG
ACGAGCCGCTTCAACGGCACGAAGCTGCTGATCCGCGCGCTGTCGCAGCAGGGGACGGTGCGGCGCAAGACGTCGGCGTC
GGTCACGACGCTCAACAACCAGCCGGTGCCCGTGCAGGTCGCGACGCAGACGGGCTACCTCGCGTCGGTGTCGACGACGA
ACACCGCGAACGTCGGCTCGTCGACGGCGCTCACGCCGGGCGTCGTGACGACGGGCTTCAACATGACGCTGCTGCCGCAC
GTGCTCGACGACGGCACCGTGATGCTGCAGTTCTCGACCAACATCTCGTCGCTGCTCGAGCTGAAGGAGGTGACGAGCAG
CACCGGCGGCAGCGGCCTGGCGCGCATCCAGACGCCCGACGTCGACATGCGCAACTTCCTGCAGCGCGTCGCGATGAAAT
CGGGCGAGACGCTTGTCATCAGCGGCTACGAAGGCGCGAACGATTCGCTCGACGAGCGCGGCGTGGGCACGCCGAAGATG
ATCGCGCTCGGCGGCGGCTACGAGGCGCAGCGCGCGCGCGAGGTGATCGTGATCCTGATCACGCCCGTCACGCAGCGCGG
CGGCGCCTGA

Upstream 100 bases:

>100_bases
AGGCGCTCGTCGCGAGCCTCGGCCGTACGTCGACGCCGATCCAGGCGATTCTCTATCGAGGCAATCACGTGTTGCGCGTC
GTCGCGCAAGGAGCGGGCTG

Downstream 100 bases:

>100_bases
CGGAGCCGGCGATGAGCGCGCAGGTGATTCAAATCGGCCGCCAGCGTTTCGTCGGCGGCCTGTTCTGGCAATCGTTGTCG
AGGCGCAACGAGTTGCGCGC

Product: type IV pilus biosynthesis protein

Products: NA

Alternate protein names: Bundle-forming pilus B [H]

Number of amino acids: Translated: 589; Mature: 589

Protein sequence:

>589_residues
MRVLFAISLLAVAWLAGCTGLRGGIERDARRDSNETGALLKRASDGDNSVRSLAPVVVDNGLWVSAGTVKLRNSEQLPPL
FDEPASFDRSVASLSEFAEQITRLTQVPTQVAASAQQAAARSQHGGAGDAARGAPAFLDASGARAVPPLPPGLPGGASAG
GGKTGGGAAGAGSEGGGTSFAPVRVLYAGGTLRGLLDAACARFGVSWKYEQGAIRFFFTDTRTFQVNAIPGDSSLNASVV
SGATSDGAAGGSQSGGSGGSGGGINGAGSGTTGLTANNTANTAVNSQLSVFNGLQSAIQSMLSRYGSSVASPATGSISVT
DTPDVLERVAAFMAQQNRSLSRQVLLNVTVLSVSLQAGDAYGIDWNLVYKTMSAGFGITNPFHPTALTAPADMSAAVLSP
TSRFNGTKLLIRALSQQGTVRRKTSASVTTLNNQPVPVQVATQTGYLASVSTTNTANVGSSTALTPGVVTTGFNMTLLPH
VLDDGTVMLQFSTNISSLLELKEVTSSTGGSGLARIQTPDVDMRNFLQRVAMKSGETLVISGYEGANDSLDERGVGTPKM
IALGGGYEAQRAREVIVILITPVTQRGGA

Sequences:

>Translated_589_residues
MRVLFAISLLAVAWLAGCTGLRGGIERDARRDSNETGALLKRASDGDNSVRSLAPVVVDNGLWVSAGTVKLRNSEQLPPL
FDEPASFDRSVASLSEFAEQITRLTQVPTQVAASAQQAAARSQHGGAGDAARGAPAFLDASGARAVPPLPPGLPGGASAG
GGKTGGGAAGAGSEGGGTSFAPVRVLYAGGTLRGLLDAACARFGVSWKYEQGAIRFFFTDTRTFQVNAIPGDSSLNASVV
SGATSDGAAGGSQSGGSGGSGGGINGAGSGTTGLTANNTANTAVNSQLSVFNGLQSAIQSMLSRYGSSVASPATGSISVT
DTPDVLERVAAFMAQQNRSLSRQVLLNVTVLSVSLQAGDAYGIDWNLVYKTMSAGFGITNPFHPTALTAPADMSAAVLSP
TSRFNGTKLLIRALSQQGTVRRKTSASVTTLNNQPVPVQVATQTGYLASVSTTNTANVGSSTALTPGVVTTGFNMTLLPH
VLDDGTVMLQFSTNISSLLELKEVTSSTGGSGLARIQTPDVDMRNFLQRVAMKSGETLVISGYEGANDSLDERGVGTPKM
IALGGGYEAQRAREVIVILITPVTQRGGA
>Mature_589_residues
MRVLFAISLLAVAWLAGCTGLRGGIERDARRDSNETGALLKRASDGDNSVRSLAPVVVDNGLWVSAGTVKLRNSEQLPPL
FDEPASFDRSVASLSEFAEQITRLTQVPTQVAASAQQAAARSQHGGAGDAARGAPAFLDASGARAVPPLPPGLPGGASAG
GGKTGGGAAGAGSEGGGTSFAPVRVLYAGGTLRGLLDAACARFGVSWKYEQGAIRFFFTDTRTFQVNAIPGDSSLNASVV
SGATSDGAAGGSQSGGSGGSGGGINGAGSGTTGLTANNTANTAVNSQLSVFNGLQSAIQSMLSRYGSSVASPATGSISVT
DTPDVLERVAAFMAQQNRSLSRQVLLNVTVLSVSLQAGDAYGIDWNLVYKTMSAGFGITNPFHPTALTAPADMSAAVLSP
TSRFNGTKLLIRALSQQGTVRRKTSASVTTLNNQPVPVQVATQTGYLASVSTTNTANVGSSTALTPGVVTTGFNMTLLPH
VLDDGTVMLQFSTNISSLLELKEVTSSTGGSGLARIQTPDVDMRNFLQRVAMKSGETLVISGYEGANDSLDERGVGTPKM
IALGGGYEAQRAREVIVILITPVTQRGGA

Specific function: Is absolutely required for pilus biogenesis, and for EPEC localized adherence and autoaggregation. Acts at a step in the BFP biogenic pathway after production and processing of the structural pilus subunit BfpA [H]

COG id: COG1450

COG function: function code NU; Type II secretory pathway, component PulD

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011514
- InterPro:   IPR004846 [H]

Pfam domain/function: PF00263 Secretin; PF07655 Secretin_N_2 [H]

EC number: NA

Molecular weight: Translated: 59924; Mature: 59924

Theoretical pI: Translated: 9.01; Mature: 9.01

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVLFAISLLAVAWLAGCTGLRGGIERDARRDSNETGALLKRASDGDNSVRSLAPVVVDN
CHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCHHHHHHHCCCCCCHHHHHCCEEECC
GLWVSAGTVKLRNSEQLPPLFDEPASFDRSVASLSEFAEQITRLTQVPTQVAASAQQAAA
CEEEEECEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RSQHGGAGDAARGAPAFLDASGARAVPPLPPGLPGGASAGGGKTGGGAAGAGSEGGGTSF
HHCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
APVRVLYAGGTLRGLLDAACARFGVSWKYEQGAIRFFFTDTRTFQVNAIPGDSSLNASVV
CEEEEEEECCHHHHHHHHHHHHHCCCEEECCCCEEEEEECCEEEEEEECCCCCCCCCCEE
SGATSDGAAGGSQSGGSGGSGGGINGAGSGTTGLTANNTANTAVNSQLSVFNGLQSAIQS
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEECCCCCCHHHCCHHHHHHHHHHHHHH
MLSRYGSSVASPATGSISVTDTPDVLERVAAFMAQQNRSLSRQVLLNVTVLSVSLQAGDA
HHHHHCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHCCCCCHHHEEEEEEEEEEEECCCC
YGIDWNLVYKTMSAGFGITNPFHPTALTAPADMSAAVLSPTSRFNGTKLLIRALSQQGTV
CCCCHHHHHHHHHCCCCCCCCCCCCEECCCCCCCHHEECCCCCCCCHHHHHHHHHHCCCE
RRKTSASVTTLNNQPVPVQVATQTGYLASVSTTNTANVGSSTALTPGVVTTGFNMTLLPH
EECCCCEEEEECCCCCEEEEECCCCEEEEECCCCCCCCCCCCCCCCCEEECCCCCEEHHH
VLDDGTVMLQFSTNISSLLELKEVTSSTGGSGLARIQTPDVDMRNFLQRVAMKSGETLVI
CCCCCEEEEEECCCHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHCCCCEEEE
SGYEGANDSLDERGVGTPKMIALGGGYEAQRAREVIVILITPVTQRGGA
ECCCCCCCCCHHCCCCCCEEEEECCCCCCCCCCEEEEEEEECCCCCCCC
>Mature Secondary Structure
MRVLFAISLLAVAWLAGCTGLRGGIERDARRDSNETGALLKRASDGDNSVRSLAPVVVDN
CHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCHHHHHHHCCCCCCHHHHHCCEEECC
GLWVSAGTVKLRNSEQLPPLFDEPASFDRSVASLSEFAEQITRLTQVPTQVAASAQQAAA
CEEEEECEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RSQHGGAGDAARGAPAFLDASGARAVPPLPPGLPGGASAGGGKTGGGAAGAGSEGGGTSF
HHCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
APVRVLYAGGTLRGLLDAACARFGVSWKYEQGAIRFFFTDTRTFQVNAIPGDSSLNASVV
CEEEEEEECCHHHHHHHHHHHHHCCCEEECCCCEEEEEECCEEEEEEECCCCCCCCCCEE
SGATSDGAAGGSQSGGSGGSGGGINGAGSGTTGLTANNTANTAVNSQLSVFNGLQSAIQS
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEECCCCCCHHHCCHHHHHHHHHHHHHH
MLSRYGSSVASPATGSISVTDTPDVLERVAAFMAQQNRSLSRQVLLNVTVLSVSLQAGDA
HHHHHCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHCCCCCHHHEEEEEEEEEEEECCCC
YGIDWNLVYKTMSAGFGITNPFHPTALTAPADMSAAVLSPTSRFNGTKLLIRALSQQGTV
CCCCHHHHHHHHHCCCCCCCCCCCCEECCCCCCCHHEECCCCCCCCHHHHHHHHHHCCCE
RRKTSASVTTLNNQPVPVQVATQTGYLASVSTTNTANVGSSTALTPGVVTTGFNMTLLPH
EECCCCEEEEECCCCCEEEEECCCCEEEEECCCCCCCCCCCCCCCCCEEECCCCCEEHHH
VLDDGTVMLQFSTNISSLLELKEVTSSTGGSGLARIQTPDVDMRNFLQRVAMKSGETLVI
CCCCCEEEEEECCCHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHCCCCEEEE
SGYEGANDSLDERGVGTPKMIALGGGYEAQRAREVIVILITPVTQRGGA
ECCCCCCCCCHHCCCCCCEEEEECCCCCCCCCCEEEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8626330; 10496929; 8932312 [H]