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The map label for this gene is ahpF

Identifier: 53721527

GI number: 53721527

Start: 667638

End: 669245

Strand: Reverse

Name: ahpF

Synonym: BPSS0491

Alternate gene names: 53721527

Gene position: 669245-667638 (Counterclockwise)

Preceding gene: 53721528

Following gene: 53721515

Centisome position: 21.09

GC content: 68.47

Gene sequence:

>1608_bases
ATGCTCGACGCCCATCTCAAGACTCAGTTGAAATCGTACCTCGAACGGATCACGCGCCCCGTCGAGCTCGTCGCATCGCT
CGACGCAGGCGACCAGTCGCGCGAGCTGCGCGCGCTGCTCGACGAGATCGCGGCGCTGTCGCCGCAGATCTTCGTGACCG
AACGCCGCGACGCGGCCGAGCGCGCGCCGTCGTTCTCGGTCGGCGAGCCGGGCAAGGCCGCGGGCATTCGCTTCGCCGGT
ATGCCGATGGGCCACGAATTCACGTCGCTCGTGCTCGCACTGCTGCAGACGGGCGGCCATCCGATCAAGCTCGACGACGC
GACGATCGAGCAGATTCGCGCGCTCGACGGCGACTACGCATTCGAGACGTATATCTCGCTCACCTGCCAGAACTGCCCGG
AAGTCGTACAGGCGCTCAACGTGATGGCGCTCGTCAACCCGCGCATCCGCCACGTGATGATCGACGGCGCGCTGTTCCAG
GACGAAGTCGAGGCGCGCCGGATCATGGCCGTGCCGACGATCTTCCTGAACGGCGAGACGTTCGGCCAGGGCCGCAGCAG
CGTGAAGGAGATCCTCGCGAAACTCGACGGCGGCGCGAGCCGGCGCGCGGCGCAGGCGCTCGCGGAGAAACCCGTGTTCG
ACATGCTGATCGTCGGCGGCGGCCCCGCGGGCGCGGCGGCGGCGATCTACGCGGCGCGCAAGGGCATCTCGACGGGCGTC
GTCGCCGAGCGCTTCGGCGGCCAGGTGCTCGATACGATGGCGATCGAGAATTTCGTGTCGGTCCAGGAAACCGAGGGGCC
GAAGTTCGCGACCGCGCTCGAGCAGCACGTGAAGCAATACGACGTCGACGTGATCGACGTAGAGCGCGCCGACCGGCTCG
TGCCGGGCCGCGTTCATGAAATTCGCCTCGCGAGCGGCGCGGTGCTGAAGGCGAAGACCGTCGTGCTCGCGACGGGCGCG
CGCTGGCGCGAGATCGGCGTGCCCGGCGAGCGCGAGTATCGCAACCGCGGCGTCGCGTACTGCCCGCATTGCGACGGGCC
GCTCTTCAAGGGCAAGCGCGTCGCGGTGGTCGGCGGCGGCAACTCGGGTGTCGAGGCGGCGATCGATCTCGCCGGCCTTG
CCGCCGCCGTGACGCTGATCGAATACGGCGAGCAGTTGCATGCGGACGACGTGCTGCAACGCAAACTGCGCAGCCTCGCG
AACGTGACGGTGATCACGCAGGCACGCACGACCGAGCTCACGGGCGACGGCGCGAAGCTGAACGGGCTCGTCTACGAGGA
CAGGCGCACGGGCGAGGCGAAGCGCATCGCGCTCGAAGGCGTGTTCGTGCAGATCGGCCTCGTGCCGAACACCGAATGGC
TGAAGGAAACCGTCGAGCTGTCGAGGCACGGCGAGATCGTCGTCGACACGCGCGGCGCGACGTCGGTGCCCGGCGTGTTC
GCCGCCGGCGACGTGACGACGGTGCCGTACAAGCAGATCGTGATCGCGGTCGGCGAAGGCGCGAAGGCATCGCTCGGCGC
ATTCGACCACCTGATTCGAAGCGAGGCGCATGCCGTGCAGGCGGCGGATATCGAAGCTTCGGCAAACGCCGAGGCCATGG
CCGCCTGA

Upstream 100 bases:

>100_bases
CGCCCGTTTCGCGGCCCCGCTCCCGGCCTCACCCGCTCCATCGCCGCCTCGCCCGCGTTCGACGGGCAAGGCCCCCTTTC
GCTATGGAATCCGAATCGTC

Downstream 100 bases:

>100_bases
TTCGCGAAACGGGCCTTTCAACCGTCTACCGTTGGCCCTCGCCGCGATGTGATGAGCGTCGTGGCGGGGGCGCTTTTTGA
CGCATGGGCACTTTTTGCCC

Product: alkyl hydroperoxide reductase subunit

Products: an alcohol; H2O; NAD [C]

Alternate protein names: NA

Number of amino acids: Translated: 535; Mature: 535

Protein sequence:

>535_residues
MLDAHLKTQLKSYLERITRPVELVASLDAGDQSRELRALLDEIAALSPQIFVTERRDAAERAPSFSVGEPGKAAGIRFAG
MPMGHEFTSLVLALLQTGGHPIKLDDATIEQIRALDGDYAFETYISLTCQNCPEVVQALNVMALVNPRIRHVMIDGALFQ
DEVEARRIMAVPTIFLNGETFGQGRSSVKEILAKLDGGASRRAAQALAEKPVFDMLIVGGGPAGAAAAIYAARKGISTGV
VAERFGGQVLDTMAIENFVSVQETEGPKFATALEQHVKQYDVDVIDVERADRLVPGRVHEIRLASGAVLKAKTVVLATGA
RWREIGVPGEREYRNRGVAYCPHCDGPLFKGKRVAVVGGGNSGVEAAIDLAGLAAAVTLIEYGEQLHADDVLQRKLRSLA
NVTVITQARTTELTGDGAKLNGLVYEDRRTGEAKRIALEGVFVQIGLVPNTEWLKETVELSRHGEIVVDTRGATSVPGVF
AAGDVTTVPYKQIVIAVGEGAKASLGAFDHLIRSEAHAVQAADIEASANAEAMAA

Sequences:

>Translated_535_residues
MLDAHLKTQLKSYLERITRPVELVASLDAGDQSRELRALLDEIAALSPQIFVTERRDAAERAPSFSVGEPGKAAGIRFAG
MPMGHEFTSLVLALLQTGGHPIKLDDATIEQIRALDGDYAFETYISLTCQNCPEVVQALNVMALVNPRIRHVMIDGALFQ
DEVEARRIMAVPTIFLNGETFGQGRSSVKEILAKLDGGASRRAAQALAEKPVFDMLIVGGGPAGAAAAIYAARKGISTGV
VAERFGGQVLDTMAIENFVSVQETEGPKFATALEQHVKQYDVDVIDVERADRLVPGRVHEIRLASGAVLKAKTVVLATGA
RWREIGVPGEREYRNRGVAYCPHCDGPLFKGKRVAVVGGGNSGVEAAIDLAGLAAAVTLIEYGEQLHADDVLQRKLRSLA
NVTVITQARTTELTGDGAKLNGLVYEDRRTGEAKRIALEGVFVQIGLVPNTEWLKETVELSRHGEIVVDTRGATSVPGVF
AAGDVTTVPYKQIVIAVGEGAKASLGAFDHLIRSEAHAVQAADIEASANAEAMAA
>Mature_535_residues
MLDAHLKTQLKSYLERITRPVELVASLDAGDQSRELRALLDEIAALSPQIFVTERRDAAERAPSFSVGEPGKAAGIRFAG
MPMGHEFTSLVLALLQTGGHPIKLDDATIEQIRALDGDYAFETYISLTCQNCPEVVQALNVMALVNPRIRHVMIDGALFQ
DEVEARRIMAVPTIFLNGETFGQGRSSVKEILAKLDGGASRRAAQALAEKPVFDMLIVGGGPAGAAAAIYAARKGISTGV
VAERFGGQVLDTMAIENFVSVQETEGPKFATALEQHVKQYDVDVIDVERADRLVPGRVHEIRLASGAVLKAKTVVLATGA
RWREIGVPGEREYRNRGVAYCPHCDGPLFKGKRVAVVGGGNSGVEAAIDLAGLAAAVTLIEYGEQLHADDVLQRKLRSLA
NVTVITQARTTELTGDGAKLNGLVYEDRRTGEAKRIALEGVFVQIGLVPNTEWLKETVELSRHGEIVVDTRGATSVPGVF
AAGDVTTVPYKQIVIAVGEGAKASLGAFDHLIRSEAHAVQAADIEASANAEAMAA

Specific function: Serves to protect the cell against DNA damage by alkyl hydroperoxides. It can use either NADH or NADPH as electron donor for direct reduction of redox dyes or of alkyl hydroperoxides when combined with the AhpC protein [H]

COG id: COG3634

COG function: function code O; Alkyl hydroperoxide reductase, large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI87081763, Length=519, Percent_Identity=60.5009633911368, Blast_Score=649, Evalue=0.0,
Organism=Escherichia coli, GI1787114, Length=310, Percent_Identity=31.9354838709677, Blast_Score=147, Evalue=2e-36,
Organism=Saccharomyces cerevisiae, GI6321898, Length=312, Percent_Identity=32.0512820512821, Blast_Score=139, Evalue=1e-33,
Organism=Saccharomyces cerevisiae, GI6320560, Length=312, Percent_Identity=30.4487179487179, Blast_Score=135, Evalue=2e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012081
- InterPro:   IPR013027
- InterPro:   IPR002109
- InterPro:   IPR008255
- InterPro:   IPR001327
- InterPro:   IPR000103
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.6.4.- [C]

Molecular weight: Translated: 57083; Mature: 57083

Theoretical pI: Translated: 5.54; Mature: 5.54

Prosite motif: PS00573 PYRIDINE_REDOX_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDAHLKTQLKSYLERITRPVELVASLDAGDQSRELRALLDEIAALSPQIFVTERRDAAE
CCCHHHHHHHHHHHHHHCHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCEEEECCHHHHH
RAPSFSVGEPGKAAGIRFAGMPMGHEFTSLVLALLQTGGHPIKLDDATIEQIRALDGDYA
HCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHCCCCEEEECHHHHHHHHHCCCCCE
FETYISLTCQNCPEVVQALNVMALVNPRIRHVMIDGALFQDEVEARRIMAVPTIFLNGET
EEHHHHHHHCCCHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHEEEEEEEEECCCC
FGQGRSSVKEILAKLDGGASRRAAQALAEKPVFDMLIVGGGPAGAAAAIYAARKGISTGV
CCCCHHHHHHHHHHCCCCCHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHCCCCCCH
VAERFGGQVLDTMAIENFVSVQETEGPKFATALEQHVKQYDVDVIDVERADRLVPGRVHE
HHHHHCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCEEEHHHHCCCCCCCCEE
IRLASGAVLKAKTVVLATGARWREIGVPGEREYRNRGVAYCPHCDGPLFKGKRVAVVGGG
EEECCCCEEEEEEEEEECCCCEEECCCCCCHHHHCCCCEECCCCCCCCCCCCEEEEEECC
NSGVEAAIDLAGLAAAVTLIEYGEQLHADDVLQRKLRSLANVTVITQARTTELTGDGAKL
CCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCEEEEEECCEEEECCCCCEE
NGLVYEDRRTGEAKRIALEGVFVQIGLVPNTEWLKETVELSRHGEIVVDTRGATSVPGVF
CCEEEECCCCCCHHEEEEEEEEEEEEECCCHHHHHHHHHHHCCCCEEEECCCCCCCCCEE
AAGDVTTVPYKQIVIAVGEGAKASLGAFDHLIRSEAHAVQAADIEASANAEAMAA
ECCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHEEEECCCCCCCCHHHCC
>Mature Secondary Structure
MLDAHLKTQLKSYLERITRPVELVASLDAGDQSRELRALLDEIAALSPQIFVTERRDAAE
CCCHHHHHHHHHHHHHHCHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCEEEECCHHHHH
RAPSFSVGEPGKAAGIRFAGMPMGHEFTSLVLALLQTGGHPIKLDDATIEQIRALDGDYA
HCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHCCCCEEEECHHHHHHHHHCCCCCE
FETYISLTCQNCPEVVQALNVMALVNPRIRHVMIDGALFQDEVEARRIMAVPTIFLNGET
EEHHHHHHHCCCHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHEEEEEEEEECCCC
FGQGRSSVKEILAKLDGGASRRAAQALAEKPVFDMLIVGGGPAGAAAAIYAARKGISTGV
CCCCHHHHHHHHHHCCCCCHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHCCCCCCH
VAERFGGQVLDTMAIENFVSVQETEGPKFATALEQHVKQYDVDVIDVERADRLVPGRVHE
HHHHHCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCEEEHHHHCCCCCCCCEE
IRLASGAVLKAKTVVLATGARWREIGVPGEREYRNRGVAYCPHCDGPLFKGKRVAVVGGG
EEECCCCEEEEEEEEEECCCCEEECCCCCCHHHHCCCCEECCCCCCCCCCCCEEEEEECC
NSGVEAAIDLAGLAAAVTLIEYGEQLHADDVLQRKLRSLANVTVITQARTTELTGDGAKL
CCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCEEEEEECCEEEECCCCCEE
NGLVYEDRRTGEAKRIALEGVFVQIGLVPNTEWLKETVELSRHGEIVVDTRGATSVPGVF
CCEEEECCCCCCHHEEEEEEEEEEEEECCCHHHHHHHHHHHCCCCEEEECCCCCCCCCEE
AAGDVTTVPYKQIVIAVGEGAKASLGAFDHLIRSEAHAVQAADIEASANAEAMAA
ECCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHEEEECCCCCCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ROOH; Proton; NADH [C]

Specific reaction: ROOH + Proton + NADH = an alcohol + H2O + NAD [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12534463 [H]