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The map label for this gene is fadD25 [H]

Identifier: 53721334

GI number: 53721334

Start: 401900

End: 403765

Strand: Reverse

Name: fadD25 [H]

Synonym: BPSS0299

Alternate gene names: 53721334

Gene position: 403765-401900 (Counterclockwise)

Preceding gene: 53721335

Following gene: 53721333

Centisome position: 12.73

GC content: 71.28

Gene sequence:

>1866_bases
ATGTCATCCATCCAATCCGCCCTCGCCGCCTGCCGCTCGCTCACCGCGCCGCCGCCGCTCGCGCCCGCGGCCAATTTCGC
CGACGTGCTGCGCTATCGCGCCGAGACGACGCCCGACGAATTCGCATACGGCTACCTCGGCTTCGGCCGCATGCCGGACC
GCGTGATGCGCTACGGCGACATTCACCGCCGCGCGCTCGGGATCGCGCGCGAGATCGTCGCGCACGGCCGCCCGGCAGAC
CCGGTGCTGCTGATCTTCCCGTCCGCCGCCGATTTCGTCGAAGCGTTCTTCGGCTGCCTGTACGCGGGCCGGATGGCCGT
GCCGGCGCTGCCGCCGCGCACCGAGAAGGAGCGCCGCCGGCTGATCTCGATCGCGCGCGACTGCGCGCCGTCGTTCGCGA
TCTGCGGCAACGGCGAGATGGACGCCGTGCTCGCCGAGCTGTGCGCGGCGGGCGTCGTCGCGCCGCCGTGCCGCGAAGTG
GGCGCGATTGCCGACGGCGGCGACATCGGCGACGCGCCGGGCGCGCTGCCCGCCATCGCGCCCGGGAGCATCGCGTTCCT
TCAGTACACGTCGGGCTCGACGTCCGATCCGAAGGGCGTGATGGTCGGCCACGACAACCTGCTCGCGAACGAGCGGCTGC
TGCGCCGGCACTGGGGCAGCGACCGCGAGCGCTGGCTGATCGTGTCGTGGCTGCCGCATTATCACGACATGGGGCTGATC
GGCGGAATCCTGCAGCCGATCTACGCGGGGCGGCCCGCGGTGTTCATGTCGCCGCAGGACTTCCTCCAGCACCCGGCGCG
CTGGCTGCACGCGGTGTCCGACTATGGCGCGACCTGCAGCGGCGCGCCGAATTTCGGCTACGAGCTGTGCCGGCGCCGCG
CGAGCCGGATGGACCTCGCGCGGCTCGATCTGTCGACGTGGGAGCAGGCGTTCAATGGCGCGGAGCCGGTGCGGCCGCGC
ACGCTGCGCGAATTCGCCGACGCGTTCGCCTGCGCCGGCTTTCGCTACGATGCGTTCGCGCCGTGCTACGGGCTCGCGGA
GCTCACGCTCGCCGCGACGTCGAAACAGATCGGCGAGAGCGTCGTCATTCGGCGCGCGGATCGCGCGGCGCTCGCGGCCG
GACGCTTCGAGCCGCAGCAAGACGGCGGCCGCTCGGTCGACGCCGTGTCGGTCGGCGCGCTCGAGCACGCGCACCAGACG
TTTTGCATCGTCGATCCGGCGACGGGCGAGCCGCAACCGACGGGTGCGATCGGCGAGATCTGCGTCGCGAGCGACAGCGT
GTGCCACGGCTATTTCGGCCGGCCGGACGCGACCGAGGCGACCTTCCACGCCTACCGGTCCAGCGCGTTTCCCGACGGCA
TGCTGCGCACCGGCGACCTCGGTTTCATCGACGAAGCGGGCCACCTGTTCGTCTCCGGGCGGATCAAGGATCTGATCATC
CTGAACGGCGTCAACTACTACCCGCAGGACATCGAGGGCGCGGTGCTGAACGTGTCCGACCAGATCCGCGCGAACCGGCT
TGCCGCGATCATGGTCGAGCGCGACGAACAGGCGGGCGTCGTCGTCGTGCTCGAGGCGATCGGGCGCTTCGATCTCGCGG
CGCTCGCGCCGGAGATCGCCCGCGAAGTGTGGGACGCGTGCCAGCTGACGCTGAGCGGCGTGATCCGCGTGAAGAAGGGC
GAGATCCATACGACGTCGAGCGGCAAGATCCAGCGCGCGACCTGCGCGAAGATGCTCGCGGACGGCGCGTTCACGATCGA
GGACGCGCATCTGCACGACACCGCGCGAGCCTGGCTCGCGCCGGTGGTCGAGCGATGCGGACGCGCCTGCCGCGAGCCTT
CGGCGGACACGCCGGCCGTGGCCTGA

Upstream 100 bases:

>100_bases
CGCCGGATCCTCACGCGCCTCGTCGACGCGATCCGGCTCGAACCGCCCGAGCGGCACGCCGCGCCGCAACCCGCACTCCC
TTGAAGAGGACCGATCCGAC

Downstream 100 bases:

>100_bases
GCGCGCGGGAGCGGCGCGGCATCGCATGGGCCATGCGGCGCCGATGCCGCGCGAGGCGATGCGTCACCATGCGGCTGACG
TCAAAACGCGCGACGCGCCG

Product: fatty-acid CoA ligase

Products: AMP; diphosphate +an acyl-CoA [C]

Alternate protein names: Acyl-CoA synthetase [H]

Number of amino acids: Translated: 621; Mature: 620

Protein sequence:

>621_residues
MSSIQSALAACRSLTAPPPLAPAANFADVLRYRAETTPDEFAYGYLGFGRMPDRVMRYGDIHRRALGIAREIVAHGRPAD
PVLLIFPSAADFVEAFFGCLYAGRMAVPALPPRTEKERRRLISIARDCAPSFAICGNGEMDAVLAELCAAGVVAPPCREV
GAIADGGDIGDAPGALPAIAPGSIAFLQYTSGSTSDPKGVMVGHDNLLANERLLRRHWGSDRERWLIVSWLPHYHDMGLI
GGILQPIYAGRPAVFMSPQDFLQHPARWLHAVSDYGATCSGAPNFGYELCRRRASRMDLARLDLSTWEQAFNGAEPVRPR
TLREFADAFACAGFRYDAFAPCYGLAELTLAATSKQIGESVVIRRADRAALAAGRFEPQQDGGRSVDAVSVGALEHAHQT
FCIVDPATGEPQPTGAIGEICVASDSVCHGYFGRPDATEATFHAYRSSAFPDGMLRTGDLGFIDEAGHLFVSGRIKDLII
LNGVNYYPQDIEGAVLNVSDQIRANRLAAIMVERDEQAGVVVVLEAIGRFDLAALAPEIAREVWDACQLTLSGVIRVKKG
EIHTTSSGKIQRATCAKMLADGAFTIEDAHLHDTARAWLAPVVERCGRACREPSADTPAVA

Sequences:

>Translated_621_residues
MSSIQSALAACRSLTAPPPLAPAANFADVLRYRAETTPDEFAYGYLGFGRMPDRVMRYGDIHRRALGIAREIVAHGRPAD
PVLLIFPSAADFVEAFFGCLYAGRMAVPALPPRTEKERRRLISIARDCAPSFAICGNGEMDAVLAELCAAGVVAPPCREV
GAIADGGDIGDAPGALPAIAPGSIAFLQYTSGSTSDPKGVMVGHDNLLANERLLRRHWGSDRERWLIVSWLPHYHDMGLI
GGILQPIYAGRPAVFMSPQDFLQHPARWLHAVSDYGATCSGAPNFGYELCRRRASRMDLARLDLSTWEQAFNGAEPVRPR
TLREFADAFACAGFRYDAFAPCYGLAELTLAATSKQIGESVVIRRADRAALAAGRFEPQQDGGRSVDAVSVGALEHAHQT
FCIVDPATGEPQPTGAIGEICVASDSVCHGYFGRPDATEATFHAYRSSAFPDGMLRTGDLGFIDEAGHLFVSGRIKDLII
LNGVNYYPQDIEGAVLNVSDQIRANRLAAIMVERDEQAGVVVVLEAIGRFDLAALAPEIAREVWDACQLTLSGVIRVKKG
EIHTTSSGKIQRATCAKMLADGAFTIEDAHLHDTARAWLAPVVERCGRACREPSADTPAVA
>Mature_620_residues
SSIQSALAACRSLTAPPPLAPAANFADVLRYRAETTPDEFAYGYLGFGRMPDRVMRYGDIHRRALGIAREIVAHGRPADP
VLLIFPSAADFVEAFFGCLYAGRMAVPALPPRTEKERRRLISIARDCAPSFAICGNGEMDAVLAELCAAGVVAPPCREVG
AIADGGDIGDAPGALPAIAPGSIAFLQYTSGSTSDPKGVMVGHDNLLANERLLRRHWGSDRERWLIVSWLPHYHDMGLIG
GILQPIYAGRPAVFMSPQDFLQHPARWLHAVSDYGATCSGAPNFGYELCRRRASRMDLARLDLSTWEQAFNGAEPVRPRT
LREFADAFACAGFRYDAFAPCYGLAELTLAATSKQIGESVVIRRADRAALAAGRFEPQQDGGRSVDAVSVGALEHAHQTF
CIVDPATGEPQPTGAIGEICVASDSVCHGYFGRPDATEATFHAYRSSAFPDGMLRTGDLGFIDEAGHLFVSGRIKDLIIL
NGVNYYPQDIEGAVLNVSDQIRANRLAAIMVERDEQAGVVVVLEAIGRFDLAALAPEIAREVWDACQLTLSGVIRVKKGE
IHTTSSGKIQRATCAKMLADGAFTIEDAHLHDTARAWLAPVVERCGRACREPSADTPAVA

Specific function: Esterification, Concomitant With Transport, Of Exogenous Long-Chain Fatty Acids Into Metabolically Active CoA Thioesters For Subsequent Degradation Or Incorporation Into Phospholipids. [C]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI44888818, Length=539, Percent_Identity=25.2319109461967, Blast_Score=111, Evalue=3e-24,
Organism=Homo sapiens, GI45827692, Length=559, Percent_Identity=25.9391771019678, Blast_Score=103, Evalue=5e-22,
Organism=Homo sapiens, GI225735629, Length=559, Percent_Identity=25.9391771019678, Blast_Score=103, Evalue=6e-22,
Organism=Homo sapiens, GI55749758, Length=515, Percent_Identity=24.4660194174757, Blast_Score=99, Evalue=1e-20,
Organism=Homo sapiens, GI225735625, Length=508, Percent_Identity=24.6062992125984, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI45827694, Length=510, Percent_Identity=24.3137254901961, Blast_Score=87, Evalue=4e-17,
Organism=Homo sapiens, GI45827696, Length=481, Percent_Identity=24.5322245322245, Blast_Score=87, Evalue=5e-17,
Organism=Homo sapiens, GI225735627, Length=481, Percent_Identity=24.5322245322245, Blast_Score=87, Evalue=5e-17,
Organism=Homo sapiens, GI45827698, Length=446, Percent_Identity=25.1121076233184, Blast_Score=78, Evalue=3e-14,
Organism=Homo sapiens, GI156151445, Length=311, Percent_Identity=24.1157556270096, Blast_Score=78, Evalue=3e-14,
Organism=Escherichia coli, GI1788107, Length=489, Percent_Identity=23.1083844580777, Blast_Score=97, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI17559526, Length=560, Percent_Identity=23.75, Blast_Score=110, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI193202679, Length=530, Percent_Identity=23.5849056603774, Blast_Score=102, Evalue=8e-22,
Organism=Drosophila melanogaster, GI24654656, Length=468, Percent_Identity=24.1452991452991, Blast_Score=87, Evalue=5e-17,
Organism=Drosophila melanogaster, GI21355181, Length=400, Percent_Identity=25, Blast_Score=79, Evalue=8e-15,
Organism=Drosophila melanogaster, GI24581924, Length=344, Percent_Identity=26.453488372093, Blast_Score=79, Evalue=1e-14,
Organism=Drosophila melanogaster, GI18859661, Length=404, Percent_Identity=24.5049504950495, Blast_Score=77, Evalue=4e-14,
Organism=Drosophila melanogaster, GI281365686, Length=171, Percent_Identity=25.1461988304094, Blast_Score=70, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.2.1.3 [C]

Molecular weight: Translated: 66832; Mature: 66700

Theoretical pI: Translated: 6.09; Mature: 6.09

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSIQSALAACRSLTAPPPLAPAANFADVLRYRAETTPDEFAYGYLGFGRMPDRVMRYGD
CCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHCCCCCCHHHHHHHHH
IHRRALGIAREIVAHGRPADPVLLIFPSAADFVEAFFGCLYAGRMAVPALPPRTEKERRR
HHHHHHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHH
LISIARDCAPSFAICGNGEMDAVLAELCAAGVVAPPCREVGAIADGGDIGDAPGALPAIA
HHHHHHHCCCCEEEECCCCHHHHHHHHHHCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCC
PGSIAFLQYTSGSTSDPKGVMVGHDNLLANERLLRRHWGSDRERWLIVSWLPHYHDMGLI
CCCEEEEEECCCCCCCCCCEEEECCHHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHH
GGILQPIYAGRPAVFMSPQDFLQHPARWLHAVSDYGATCSGAPNFGYELCRRRASRMDLA
HHHHHHHHCCCCEEEECHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHH
RLDLSTWEQAFNGAEPVRPRTLREFADAFACAGFRYDAFAPCYGLAELTLAATSKQIGES
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
VVIRRADRAALAAGRFEPQQDGGRSVDAVSVGALEHAHQTFCIVDPATGEPQPTGAIGEI
HHHCCCHHHHHHHCCCCCCCCCCCCCCCEEHHHHHHCCCEEEEEECCCCCCCCCCCHHHH
CVASDSVCHGYFGRPDATEATFHAYRSSAFPDGMLRTGDLGFIDEAGHLFVSGRIKDLII
HHCCCCHHCCCCCCCCCCHHHHHHHHHCCCCCCCEECCCCCCEECCCCEEEECCCEEEEE
LNGVNYYPQDIEGAVLNVSDQIRANRLAAIMVERDEQAGVVVVLEAIGRFDLAALAPEIA
ECCCCCCCCCCCCEEECCHHHHHHHCEEEEEEECCCCCCEEEEEHHHCCHHHHHHHHHHH
REVWDACQLTLSGVIRVKKGEIHTTSSGKIQRATCAKMLADGAFTIEDAHLHDTARAWLA
HHHHHHHHHHHHCEEEEECCCEEECCCCCCHHHHHHHHHHCCCEEEECCHHHHHHHHHHH
PVVERCGRACREPSADTPAVA
HHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
SSIQSALAACRSLTAPPPLAPAANFADVLRYRAETTPDEFAYGYLGFGRMPDRVMRYGD
CHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHCCCCCCHHHHHHHHH
IHRRALGIAREIVAHGRPADPVLLIFPSAADFVEAFFGCLYAGRMAVPALPPRTEKERRR
HHHHHHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHH
LISIARDCAPSFAICGNGEMDAVLAELCAAGVVAPPCREVGAIADGGDIGDAPGALPAIA
HHHHHHHCCCCEEEECCCCHHHHHHHHHHCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCC
PGSIAFLQYTSGSTSDPKGVMVGHDNLLANERLLRRHWGSDRERWLIVSWLPHYHDMGLI
CCCEEEEEECCCCCCCCCCEEEECCHHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHH
GGILQPIYAGRPAVFMSPQDFLQHPARWLHAVSDYGATCSGAPNFGYELCRRRASRMDLA
HHHHHHHHCCCCEEEECHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHH
RLDLSTWEQAFNGAEPVRPRTLREFADAFACAGFRYDAFAPCYGLAELTLAATSKQIGES
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
VVIRRADRAALAAGRFEPQQDGGRSVDAVSVGALEHAHQTFCIVDPATGEPQPTGAIGEI
HHHCCCHHHHHHHCCCCCCCCCCCCCCCEEHHHHHHCCCEEEEEECCCCCCCCCCCHHHH
CVASDSVCHGYFGRPDATEATFHAYRSSAFPDGMLRTGDLGFIDEAGHLFVSGRIKDLII
HHCCCCHHCCCCCCCCCCHHHHHHHHHCCCCCCCEECCCCCCEECCCCEEEECCCEEEEE
LNGVNYYPQDIEGAVLNVSDQIRANRLAAIMVERDEQAGVVVVLEAIGRFDLAALAPEIA
ECCCCCCCCCCCCEEECCHHHHHHHCEEEEEEECCCCCCEEEEEHHHCCHHHHHHHHHHH
REVWDACQLTLSGVIRVKKGEIHTTSSGKIQRATCAKMLADGAFTIEDAHLHDTARAWLA
HHHHHHHHHHHHCEEEEECCCEEECCCCCCHHHHHHHHHHCCCEEEECCHHHHHHHHHHH
PVVERCGRACREPSADTPAVA
HHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Salts [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0408 {octanoate}} 0.0125 {octanoate}} 0.0059 {octanoate}} 0.00072 {octanoate}} 0.0013 {undecanoate}} 0.0022 {pentadecanoate}} 0.053 {CoA}} 0.035 {CoA}} 0.034 {CoA}} 0.0034 {tridecanoate}} 0.002 {laurate}} 0.0019 {laurate}} 0.0

Substrates: ATP; a long-chain carboxylic acid; CoA [C]

Specific reaction: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate +an acyl-CoA [C]

General reaction: Acid-thiol ligation; Phosphorylation [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]