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The map label for this gene is gcvA [H]

Identifier: 53721170

GI number: 53721170

Start: 173732

End: 174673

Strand: Reverse

Name: gcvA [H]

Synonym: BPSS0134

Alternate gene names: 53721170

Gene position: 174673-173732 (Counterclockwise)

Preceding gene: 53721172

Following gene: 53721161

Centisome position: 5.5

GC content: 72.08

Gene sequence:

>942_bases
ATGTCCGAACAATCGAAAAATTCGCAAGCCGGGCATGCGCCGCTTGCATACCCGCTCGCCGCCCTCACGCGCGCGCTGCC
GACGCTGGCCGCGCTCCAGTCGTTCGTCGCGGCCGCGCAGCTCGGCAGTCTCAGCAAGGCCGCCGCACATCTATGCCGCA
CGCAGGGCGCGGTGAGCCGCCAGATTCAGCAGCTCGAGGCCCACTATCGATGCGCGCTGTTCGTCCGCCAGCCGACCGGA
CTGACGCTCACCGCCGACGGCGACGCGCTCTTCGCCGTGGCGGCCGACGTGCTCGCGCGGCTCGTTCGGCACGCGCGCGA
GCGCGACGAAGCGGCGGCGGCCGTCACCGTGCGCGTGCCCTCGACATTCGCGATTCGATGGCTGCTGCCGCGCCTGCCGG
CGATTCGGCGGGCGCTCGGCGCGACGCAATTGTGCCTGTCGACGTCGGCGAACGACACGCCGGATTTCTCCGAGCCGGAC
ATCGATGCGATCGTCGCCCGCGGCGACGGGCGCTGGCCCGGCGTCGAAGCGATTCCGCTGTTCGCGGAAACGCTCGCGCC
GATGTGCGGCCCCGCCCTCGCCGCGTCGCTGAAATCGGTCGACGATCTCGCGCACGTCACGCTGCTTCATCCAGGGCGCG
GCCGCGCGGAATGGCGCTGCTGGCTCGACGCGGTCGGCGCCGCGCACATCGACGCGACGCGCGGCCCGGTATTCGACACG
CTCGAGCTGACGCTGAGCGCGGCGGCGGAAGGACACGGCGTCGCGATCGGCGATCCGCGCATGGCGGGCGACCGGCTCCG
CGCGGGCACGCTGACGATGCCGCTGCGCGAGACCGCGGCGAACGGCCTGTCGTACTACCTCGTGTATCCGGCGCAGCGCG
CGACGCAGCCGAAGATTCGCGCGCTGGCGGACGTGCTCGTCAAACTCGCGCGAGCGCGCTGA

Upstream 100 bases:

>100_bases
ATGACGAGTCCACGATCTTCAATCCTCCGATCATTCGCCGATGCGCGCCACGCGCGGCGCGAGCAGGCGCAACGGAAGCA
GCCAAGATAGCGGCGCGCAG

Downstream 100 bases:

>100_bases
GCACTCCGCGCGGCCCCGGCGCGGTCCGGGGCCGCCGCCGTCACGGCTTGACGCCTTCGATCACGCTGTTCATGCCTTGC
GTCGGGCGCACGGCCGTCAT

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 313; Mature: 312

Protein sequence:

>313_residues
MSEQSKNSQAGHAPLAYPLAALTRALPTLAALQSFVAAAQLGSLSKAAAHLCRTQGAVSRQIQQLEAHYRCALFVRQPTG
LTLTADGDALFAVAADVLARLVRHARERDEAAAAVTVRVPSTFAIRWLLPRLPAIRRALGATQLCLSTSANDTPDFSEPD
IDAIVARGDGRWPGVEAIPLFAETLAPMCGPALAASLKSVDDLAHVTLLHPGRGRAEWRCWLDAVGAAHIDATRGPVFDT
LELTLSAAAEGHGVAIGDPRMAGDRLRAGTLTMPLRETAANGLSYYLVYPAQRATQPKIRALADVLVKLARAR

Sequences:

>Translated_313_residues
MSEQSKNSQAGHAPLAYPLAALTRALPTLAALQSFVAAAQLGSLSKAAAHLCRTQGAVSRQIQQLEAHYRCALFVRQPTG
LTLTADGDALFAVAADVLARLVRHARERDEAAAAVTVRVPSTFAIRWLLPRLPAIRRALGATQLCLSTSANDTPDFSEPD
IDAIVARGDGRWPGVEAIPLFAETLAPMCGPALAASLKSVDDLAHVTLLHPGRGRAEWRCWLDAVGAAHIDATRGPVFDT
LELTLSAAAEGHGVAIGDPRMAGDRLRAGTLTMPLRETAANGLSYYLVYPAQRATQPKIRALADVLVKLARAR
>Mature_312_residues
SEQSKNSQAGHAPLAYPLAALTRALPTLAALQSFVAAAQLGSLSKAAAHLCRTQGAVSRQIQQLEAHYRCALFVRQPTGL
TLTADGDALFAVAADVLARLVRHARERDEAAAAVTVRVPSTFAIRWLLPRLPAIRRALGATQLCLSTSANDTPDFSEPDI
DAIVARGDGRWPGVEAIPLFAETLAPMCGPALAASLKSVDDLAHVTLLHPGRGRAEWRCWLDAVGAAHIDATRGPVFDTL
ELTLSAAAEGHGVAIGDPRMAGDRLRAGTLTMPLRETAANGLSYYLVYPAQRATQPKIRALADVLVKLARAR

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=286, Percent_Identity=34.2657342657343, Blast_Score=154, Evalue=5e-39,
Organism=Escherichia coli, GI1786448, Length=275, Percent_Identity=30.1818181818182, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1788706, Length=285, Percent_Identity=27.719298245614, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI145693193, Length=277, Percent_Identity=29.6028880866426, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33209; Mature: 33078

Theoretical pI: Translated: 9.02; Mature: 9.02

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEQSKNSQAGHAPLAYPLAALTRALPTLAALQSFVAAAQLGSLSKAAAHLCRTQGAVSR
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
QIQQLEAHYRCALFVRQPTGLTLTADGDALFAVAADVLARLVRHARERDEAAAAVTVRVP
HHHHHHHHCEEEEEEECCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHCHHHEEEEEECC
STFAIRWLLPRLPAIRRALGATQLCLSTSANDTPDFSEPDIDAIVARGDGRWPGVEAIPL
CHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCCCCCCCHHHH
FAETLAPMCGPALAASLKSVDDLAHVTLLHPGRGRAEWRCWLDAVGAAHIDATRGPVFDT
HHHHHHHHCCHHHHHHHHHHHHHHHEEEECCCCCCCHHHHHHHHHCHHHCCCCCCCCHHH
LELTLSAAAEGHGVAIGDPRMAGDRLRAGTLTMPLRETAANGLSYYLVYPAQRATQPKIR
HHHHHHHCCCCCCEEECCCCCCCCCCCCCEEECCHHHHHCCCCEEEEEECCCCCCCHHHH
ALADVLVKLARAR
HHHHHHHHHHHCC
>Mature Secondary Structure 
SEQSKNSQAGHAPLAYPLAALTRALPTLAALQSFVAAAQLGSLSKAAAHLCRTQGAVSR
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
QIQQLEAHYRCALFVRQPTGLTLTADGDALFAVAADVLARLVRHARERDEAAAAVTVRVP
HHHHHHHHCEEEEEEECCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHCHHHEEEEEECC
STFAIRWLLPRLPAIRRALGATQLCLSTSANDTPDFSEPDIDAIVARGDGRWPGVEAIPL
CHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCCCCCCCHHHH
FAETLAPMCGPALAASLKSVDDLAHVTLLHPGRGRAEWRCWLDAVGAAHIDATRGPVFDT
HHHHHHHHCCHHHHHHHHHHHHHHHEEEECCCCCCCHHHHHHHHHCHHHCCCCCCCCHHH
LELTLSAAAEGHGVAIGDPRMAGDRLRAGTLTMPLRETAANGLSYYLVYPAQRATQPKIR
HHHHHHHCCCCCCEEECCCCCCCCCCCCCEEECCHHHHHCCCCEEEEEECCCCCCCHHHH
ALADVLVKLARAR
HHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]