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The map label for this gene is traI [H]

Identifier: 53720874

GI number: 53720874

Start: 3880126

End: 3882111

Strand: Reverse

Name: traI [H]

Synonym: BPSL3264

Alternate gene names: 53720874

Gene position: 3882111-3880126 (Counterclockwise)

Preceding gene: 53720875

Following gene: 53720873

Centisome position: 95.28

GC content: 61.33

Gene sequence:

>1986_bases
ATGATTGCCAAGCACGTTCCCATGCGCTCGCTTGGCAAGAGTGATTTCGCGGGCTTAGTGAAGTACATCACGGCAGACCA
GGCCAAAGAGCACCGTATCGGGGAGGTACGGCTGACGAATTGCGCGGCGCTCACGGTTCGTGACGCGGTGACCGAGGTGC
TGGCCACCCAACAAGCGAACACCAGAGCCAAGAGCGACAAGACTTATCACCTCCTGGTCAGCTTCCGCGCGGGCGAACTG
CCCGATGCTGCGACGCTCGAAGCTATCGAGGCCCGGATATGTGCCGGCCTTGGGTTTAGCGATCACCAGCGCGTCAGTGC
AGTGCATCACGACACCGACAACCTGCACGTACACATTGCCATCAACAAGATTCACCCGACGCGAAACACCATTCATGAAC
CGTATTACCCGCACCGAACGTTGGCCGAGTTGTGCGACGTGCTCGAGCGGGGTTACGGGTTGCAACGCGACAACCATGCA
CCGCGCCGGCGTGGCGCCGAGTCGCGTGCGGTCGACATGGAAAGGCACTCCGGCATCGAGAGCCTGGTCGGCTGGATCAA
GCGCGAGTGCTTGGAAGAAATTCGAGGTGCGCGGAGCTGGGCTGGCCTGCACCAGATCGCGCGTGCCAACGGGCTGGAAA
TCCGGGCAAAAGGGAACGGGCTGGTGATCGAGGCCAGCAACGGTACGCAGATCAAGGTAAGCACGTTGGGCCGCGACTTC
TCAAAAGTGAAGCTGGAAGGTCGGTATGGTCCATTCGAGTCATTGCCGGTACGGCGGGCCGATGTCTCGTCGAAGCGCCA
GTATCAAAAGGATCCTATACCGCTGCGGGTGAACACCGTGGAGTTGTATGCGAAATATCAGATTGCCCAGCAGCGTGCGA
CGACCGACCGGGCGGCCGCCTTGGCCGAGCTGCGCAGCCAGAAGGAAAAGCAGATTGAGAACGCCAAGAGGAAAGGGCAG
ACCCGGCGCGCGGCAATCAAGCTGTTGGGTGGCGGCCGCCTGACGAAAAAGGTGCTGTATGCCCAAGCGAGCGGCGCCCT
GAAATCGGAGCTGCAAGCAATTTACACGCAGCATCGGGTTGCGTGCCAGCGCCTCTACGAGCAGCACCGCCGGCATACGT
GGGCCGACTGGCTCAAGCAGGAGGCTTTGCAAGGCAACGCAGAGGCCCTGACGGCGCTGCGTGCCAGAGAGGCCGCCCAA
GGCCTAAAAGGCAGCACGATAAAGGCGGAGGGACGGCCGCAACCTGGCCATGCGCCCGTAATCGACAACATCACCAAAAC
GGGAACGATCATCTATCGAGCCGGTAATAGCGCTGTGCGGGACGATGGCGACCGCTTGCAGGTATCTGGACAGGCAGGCC
AAGAGAGTGTGCAGCAGGCGCTACGGCTGGCGGTCGAGCGATACGGCGATCGCATCACGGTCGCTGGCAGCGCGGAATTC
AAAGCGCGTGTCATCCGAGCGGCAATTGATATTCGGCTTCCCATCATGTTCGATGATCCATCGATGGAGCGGTTGCGGCA
ACAGTACATGACGACCCGTCAACCTGCGCGGGTCAGTCGTCCGGCGGTGAAGCCTGTGGGTTCGGCGCCTCCTCCTCAGG
CTCGAAATCGTCTGCGCACACTGTCCCAACTCGAAGTGCCGTCGATCAAAGGTGGCGATCAAGCGGTAAGCCGTCACTCG
GTCAAGAAGCCGATCGAAAGCGATCCCGCGCGTCAGCAGGTCGAGCATGCCGTCCAACAGGCAGTTAGCCGATTTAAGGC
GGTTGCGAATCGGCGGAAGGTGCGAGCAAACGGCTATGGCGACCAAGGAAAGGCCTGGCAGAACTTGCCAGGCGAGTTGC
GAGAGCGGATCGATCGTTTCAATTCTCAGCCACCAGAGCGCCAAGAGATCGAGTTGGCACGGATGCGCCGCGAGGTCGCG
GAGCGCCGCGGCGGTGAATCGTCGTCGCTGGCCGGTGCGACACGGCAGAAAGGTCGCTCCCGATGA

Upstream 100 bases:

>100_bases
TCACTCCGGCCACGATCCGTGCGTTGCTGTCCAAGATCGAAACGATGCGGGATGAGCTGAGCCGCATCATGGAAAAAGTA
GTGCGGCCGAGGGCCGAGCC

Downstream 100 bases:

>100_bases
GGCAGAGAACCGGCAAGGCATAGACGCTAGGCGGTCCATCGACGGTCAAGTGTGCTGATACCGTGACATCACAACAAATG
AGGCCGGGCTTCGGTGTCGC

Product: conjugal transfer relaxase TraI

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 661; Mature: 661

Protein sequence:

>661_residues
MIAKHVPMRSLGKSDFAGLVKYITADQAKEHRIGEVRLTNCAALTVRDAVTEVLATQQANTRAKSDKTYHLLVSFRAGEL
PDAATLEAIEARICAGLGFSDHQRVSAVHHDTDNLHVHIAINKIHPTRNTIHEPYYPHRTLAELCDVLERGYGLQRDNHA
PRRRGAESRAVDMERHSGIESLVGWIKRECLEEIRGARSWAGLHQIARANGLEIRAKGNGLVIEASNGTQIKVSTLGRDF
SKVKLEGRYGPFESLPVRRADVSSKRQYQKDPIPLRVNTVELYAKYQIAQQRATTDRAAALAELRSQKEKQIENAKRKGQ
TRRAAIKLLGGGRLTKKVLYAQASGALKSELQAIYTQHRVACQRLYEQHRRHTWADWLKQEALQGNAEALTALRAREAAQ
GLKGSTIKAEGRPQPGHAPVIDNITKTGTIIYRAGNSAVRDDGDRLQVSGQAGQESVQQALRLAVERYGDRITVAGSAEF
KARVIRAAIDIRLPIMFDDPSMERLRQQYMTTRQPARVSRPAVKPVGSAPPPQARNRLRTLSQLEVPSIKGGDQAVSRHS
VKKPIESDPARQQVEHAVQQAVSRFKAVANRRKVRANGYGDQGKAWQNLPGELRERIDRFNSQPPERQEIELARMRREVA
ERRGGESSSLAGATRQKGRSR

Sequences:

>Translated_661_residues
MIAKHVPMRSLGKSDFAGLVKYITADQAKEHRIGEVRLTNCAALTVRDAVTEVLATQQANTRAKSDKTYHLLVSFRAGEL
PDAATLEAIEARICAGLGFSDHQRVSAVHHDTDNLHVHIAINKIHPTRNTIHEPYYPHRTLAELCDVLERGYGLQRDNHA
PRRRGAESRAVDMERHSGIESLVGWIKRECLEEIRGARSWAGLHQIARANGLEIRAKGNGLVIEASNGTQIKVSTLGRDF
SKVKLEGRYGPFESLPVRRADVSSKRQYQKDPIPLRVNTVELYAKYQIAQQRATTDRAAALAELRSQKEKQIENAKRKGQ
TRRAAIKLLGGGRLTKKVLYAQASGALKSELQAIYTQHRVACQRLYEQHRRHTWADWLKQEALQGNAEALTALRAREAAQ
GLKGSTIKAEGRPQPGHAPVIDNITKTGTIIYRAGNSAVRDDGDRLQVSGQAGQESVQQALRLAVERYGDRITVAGSAEF
KARVIRAAIDIRLPIMFDDPSMERLRQQYMTTRQPARVSRPAVKPVGSAPPPQARNRLRTLSQLEVPSIKGGDQAVSRHS
VKKPIESDPARQQVEHAVQQAVSRFKAVANRRKVRANGYGDQGKAWQNLPGELRERIDRFNSQPPERQEIELARMRREVA
ERRGGESSSLAGATRQKGRSR
>Mature_661_residues
MIAKHVPMRSLGKSDFAGLVKYITADQAKEHRIGEVRLTNCAALTVRDAVTEVLATQQANTRAKSDKTYHLLVSFRAGEL
PDAATLEAIEARICAGLGFSDHQRVSAVHHDTDNLHVHIAINKIHPTRNTIHEPYYPHRTLAELCDVLERGYGLQRDNHA
PRRRGAESRAVDMERHSGIESLVGWIKRECLEEIRGARSWAGLHQIARANGLEIRAKGNGLVIEASNGTQIKVSTLGRDF
SKVKLEGRYGPFESLPVRRADVSSKRQYQKDPIPLRVNTVELYAKYQIAQQRATTDRAAALAELRSQKEKQIENAKRKGQ
TRRAAIKLLGGGRLTKKVLYAQASGALKSELQAIYTQHRVACQRLYEQHRRHTWADWLKQEALQGNAEALTALRAREAAQ
GLKGSTIKAEGRPQPGHAPVIDNITKTGTIIYRAGNSAVRDDGDRLQVSGQAGQESVQQALRLAVERYGDRITVAGSAEF
KARVIRAAIDIRLPIMFDDPSMERLRQQYMTTRQPARVSRPAVKPVGSAPPPQARNRLRTLSQLEVPSIKGGDQAVSRHS
VKKPIESDPARQQVEHAVQQAVSRFKAVANRRKVRANGYGDQGKAWQNLPGELRERIDRFNSQPPERQEIELARMRREVA
ERRGGESSSLAGATRQKGRSR

Specific function: The initiation process of transfer DNA synthesis requires the interaction of at least three plasmid-specific components (traH, traI, and traJ) at the transfer origin resulting in the assembly of a specialized nucleoprotein complex - the relaxosome. Site a

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005094 [H]

Pfam domain/function: PF03432 Relaxase [H]

EC number: NA

Molecular weight: Translated: 73699; Mature: 73699

Theoretical pI: Translated: 10.87; Mature: 10.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIAKHVPMRSLGKSDFAGLVKYITADQAKEHRIGEVRLTNCAALTVRDAVTEVLATQQAN
CCCCCCCHHHCCCHHHHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHHHHHHHHC
TRAKSDKTYHLLVSFRAGELPDAATLEAIEARICAGLGFSDHQRVSAVHHDTDNLHVHIA
CCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCEEEEEE
INKIHPTRNTIHEPYYPHRTLAELCDVLERGYGLQRDNHAPRRRGAESRAVDMERHSGIE
EEEECCCHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHCCCCHHCCCCHHHCCHH
SLVGWIKRECLEEIRGARSWAGLHQIARANGLEIRAKGNGLVIEASNGTQIKVSTLGRDF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCEEEEECCCCCEEEEEECCCCC
SKVKLEGRYGPFESLPVRRADVSSKRQYQKDPIPLRVNTVELYAKYQIAQQRATTDRAAA
HHEEEECCCCCCCCCCCCHHCCHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHH
LAELRSQKEKQIENAKRKGQTRRAAIKLLGGGRLTKKVLYAQASGALKSELQAIYTQHRV
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHH
ACQRLYEQHRRHTWADWLKQEALQGNAEALTALRAREAAQGLKGSTIKAEGRPQPGHAPV
HHHHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCH
IDNITKTGTIIYRAGNSAVRDDGDRLQVSGQAGQESVQQALRLAVERYGDRITVAGSAEF
HCCCCCCCEEEEECCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHCCCEEEEECCHHH
KARVIRAAIDIRLPIMFDDPSMERLRQQYMTTRQPARVSRPAVKPVGSAPPPQARNRLRT
HHHHHHHHHHEEEEEEECCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
LSQLEVPSIKGGDQAVSRHSVKKPIESDPARQQVEHAVQQAVSRFKAVANRRKVRANGYG
HHHCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEECCCCC
DQGKAWQNLPGELRERIDRFNSQPPERQEIELARMRREVAERRGGESSSLAGATRQKGRS
CCCCHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHCCHHHHCCCC
R
C
>Mature Secondary Structure
MIAKHVPMRSLGKSDFAGLVKYITADQAKEHRIGEVRLTNCAALTVRDAVTEVLATQQAN
CCCCCCCHHHCCCHHHHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHHHHHHHHC
TRAKSDKTYHLLVSFRAGELPDAATLEAIEARICAGLGFSDHQRVSAVHHDTDNLHVHIA
CCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCEEEEEE
INKIHPTRNTIHEPYYPHRTLAELCDVLERGYGLQRDNHAPRRRGAESRAVDMERHSGIE
EEEECCCHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHCCCCHHCCCCHHHCCHH
SLVGWIKRECLEEIRGARSWAGLHQIARANGLEIRAKGNGLVIEASNGTQIKVSTLGRDF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCEEEEECCCCCEEEEEECCCCC
SKVKLEGRYGPFESLPVRRADVSSKRQYQKDPIPLRVNTVELYAKYQIAQQRATTDRAAA
HHEEEECCCCCCCCCCCCHHCCHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHH
LAELRSQKEKQIENAKRKGQTRRAAIKLLGGGRLTKKVLYAQASGALKSELQAIYTQHRV
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHH
ACQRLYEQHRRHTWADWLKQEALQGNAEALTALRAREAAQGLKGSTIKAEGRPQPGHAPV
HHHHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCH
IDNITKTGTIIYRAGNSAVRDDGDRLQVSGQAGQESVQQALRLAVERYGDRITVAGSAEF
HCCCCCCCEEEEECCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHCCCEEEEECCHHH
KARVIRAAIDIRLPIMFDDPSMERLRQQYMTTRQPARVSRPAVKPVGSAPPPQARNRLRT
HHHHHHHHHHEEEEEEECCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
LSQLEVPSIKGGDQAVSRHSVKKPIESDPARQQVEHAVQQAVSRFKAVANRRKVRANGYG
HHHCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEECCCCC
DQGKAWQNLPGELRERIDRFNSQPPERQEIELARMRREVAERRGGESSSLAGATRQKGRS
CCCCHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHCCHHHHCCCC
R
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1665997 [H]