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The map label for this gene is thiG

Identifier: 53720759

GI number: 53720759

Start: 3750913

End: 3751728

Strand: Reverse

Name: thiG

Synonym: BPSL3152

Alternate gene names: 53720759

Gene position: 3751728-3750913 (Counterclockwise)

Preceding gene: 53720760

Following gene: 53720758

Centisome position: 92.08

GC content: 69.49

Gene sequence:

>816_bases
ATGACGCCTCTTTCTTCCGCCGATACGCTCACGCTGCACGGCCAAACCTTCGCGAGCCGCGTGCTGCTCGGCACGTCGCG
CTATCCGTCGCTGCAATCGCTGTCCGATTCGATCGCGGCCGCGCGCCCGGGCATGGTGACGGTCGCGCTGCGTCGCCAGA
TGAACGCCGGCGCGGCCGAGGCCGGTTTCTTCGAACTGCTCAAGCGCCACGACGTGCCGCTCTTGCCGAACACGGCGGGC
TGCCAGACGATCGCCGAGGCGGTGACGACCGCACAGATGGCGCGCGAGGTATTCGAGACCGACTGGATCAAGCTCGAGCT
GATCGGCGACGACTACACGTTGCAGCCGGACCCGGTCGGCTTGATCGAAGCGGCGACGCTGCTCGTGAAGGACGGCTTCA
AGGTGCTGCCGTATTGCACCGAGGATCTCGTGATCGCCCGGCGCCTGCTCGACGCCGGCTGCGAGGCGCTGATGCCGTGG
GGCGCGCCGATCGGCACCGGCAAGGGCATCGTGAATCCGTACGGGCTGCGCGTGCTGCGCGAGCGGCTGCCGGACGTGCC
GCTCATCGTTGACGCGGGGCTCGGCGTGCCGTCGCACGCGTGCCAGGTGATGGAGTGGGGCTTCGACGGCGTGTTGCTGA
ACACGGCCGTGTCGCAGGCGACGCATCCGGAAATCATGGCGCGCGCATTCGCACGGGGCGTCGAGGCGGGGCGCGCCGCG
TATCTCGCGGGGCCGATGGATGCGCGCGAGAGCGCGCACGCGAGCACGCCCGTCGTCGGCATGCCGTTCTGGCACCAGGA
CGGGAGCCACGCATGA

Upstream 100 bases:

>100_bases
GCCGCGGGCGACAAGCTCGACATCGTGCATCCGGTCGCGGGCGGCTGACGCGCCGCCGCGCGCGCCTCGCCCCCGCTCTC
CGTTCCCACAGGATCTTCCG

Downstream 100 bases:

>100_bases
GCGCCGCGTTGCCCGACGCGTTCTGGCCGCCCGCCGACGAGCTTACCGAGGCCGCCGAGCGGATTCGCGCGACGCTCGGT
GCGTGGCAGCGGCCGGCCGT

Product: thiazole synthase

Products: 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate; 4-hydroxy-benzyl-alcohol; C1 of tyrosine; ThiS protein [C]

Alternate protein names: NA

Number of amino acids: Translated: 271; Mature: 270

Protein sequence:

>271_residues
MTPLSSADTLTLHGQTFASRVLLGTSRYPSLQSLSDSIAAARPGMVTVALRRQMNAGAAEAGFFELLKRHDVPLLPNTAG
CQTIAEAVTTAQMAREVFETDWIKLELIGDDYTLQPDPVGLIEAATLLVKDGFKVLPYCTEDLVIARRLLDAGCEALMPW
GAPIGTGKGIVNPYGLRVLRERLPDVPLIVDAGLGVPSHACQVMEWGFDGVLLNTAVSQATHPEIMARAFARGVEAGRAA
YLAGPMDARESAHASTPVVGMPFWHQDGSHA

Sequences:

>Translated_271_residues
MTPLSSADTLTLHGQTFASRVLLGTSRYPSLQSLSDSIAAARPGMVTVALRRQMNAGAAEAGFFELLKRHDVPLLPNTAG
CQTIAEAVTTAQMAREVFETDWIKLELIGDDYTLQPDPVGLIEAATLLVKDGFKVLPYCTEDLVIARRLLDAGCEALMPW
GAPIGTGKGIVNPYGLRVLRERLPDVPLIVDAGLGVPSHACQVMEWGFDGVLLNTAVSQATHPEIMARAFARGVEAGRAA
YLAGPMDARESAHASTPVVGMPFWHQDGSHA
>Mature_270_residues
TPLSSADTLTLHGQTFASRVLLGTSRYPSLQSLSDSIAAARPGMVTVALRRQMNAGAAEAGFFELLKRHDVPLLPNTAGC
QTIAEAVTTAQMAREVFETDWIKLELIGDDYTLQPDPVGLIEAATLLVKDGFKVLPYCTEDLVIARRLLDAGCEALMPWG
APIGTGKGIVNPYGLRVLRERLPDVPLIVDAGLGVPSHACQVMEWGFDGVLLNTAVSQATHPEIMARAFARGVEAGRAAY
LAGPMDARESAHASTPVVGMPFWHQDGSHA

Specific function: Catalyzes the rearrangement of 1-deoxy-D-xylulose 5- phosphate (DXP) to produce the thiazole phosphate moiety of thiamine. Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS. In vitro, sulfur can be provided by H(2)S

COG id: COG2022

COG function: function code H; Uncharacterized enzyme of thiazole biosynthesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiG family

Homologues:

Organism=Escherichia coli, GI48994993, Length=252, Percent_Identity=42.0634920634921, Blast_Score=190, Evalue=9e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIG_BURMA (Q62GC6)

Other databases:

- EMBL:   CP000010
- RefSeq:   YP_104248.1
- ProteinModelPortal:   Q62GC6
- SMR:   Q62GC6
- GeneID:   3091410
- GenomeReviews:   CP000010_GR
- KEGG:   bma:BMA2728
- TIGR:   BMA2728
- HOGENOM:   HBG296821
- OMA:   VAIRRTN
- PhylomeDB:   Q62GC6
- ProtClustDB:   PRK00208
- BioCyc:   BMAL243160:BMA_2728-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00443
- InterPro:   IPR013785
- InterPro:   IPR008867
- Gene3D:   G3DSA:3.20.20.70

Pfam domain/function: PF05690 ThiG; SSF110399 ThiG

EC number: NA

Molecular weight: Translated: 28943; Mature: 28811

Theoretical pI: Translated: 5.30; Mature: 5.30

Prosite motif: PS00133 CARBOXYPEPT_ZN_2

Important sites: ACT_SITE 104-104 BINDING 165-165

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPLSSADTLTLHGQTFASRVLLGTSRYPSLQSLSDSIAAARPGMVTVALRRQMNAGAAE
CCCCCCCCEEEECCHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCEEEHHHHHHHCCCCCH
AGFFELLKRHDVPLLPNTAGCQTIAEAVTTAQMAREVFETDWIKLELIGDDYTLQPDPVG
HHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCEEECCCCCH
LIEAATLLVKDGFKVLPYCTEDLVIARRLLDAGCEALMPWGAPIGTGKGIVNPYGLRVLR
HHHHHHHHHHCCCEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHH
ERLPDVPLIVDAGLGVPSHACQVMEWGFDGVLLNTAVSQATHPEIMARAFARGVEAGRAA
HHCCCCCEEEECCCCCCHHHHHHHHCCCCCEEHHHHHHHCCCHHHHHHHHHHHHHCCCEE
YLAGPMDARESAHASTPVVGMPFWHQDGSHA
EEECCCHHHHHCCCCCCEEECCCCCCCCCCC
>Mature Secondary Structure 
TPLSSADTLTLHGQTFASRVLLGTSRYPSLQSLSDSIAAARPGMVTVALRRQMNAGAAE
CCCCCCCEEEECCHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCEEEHHHHHHHCCCCCH
AGFFELLKRHDVPLLPNTAGCQTIAEAVTTAQMAREVFETDWIKLELIGDDYTLQPDPVG
HHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCEEECCCCCH
LIEAATLLVKDGFKVLPYCTEDLVIARRLLDAGCEALMPWGAPIGTGKGIVNPYGLRVLR
HHHHHHHHHHCCCEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHH
ERLPDVPLIVDAGLGVPSHACQVMEWGFDGVLLNTAVSQATHPEIMARAFARGVEAGRAA
HHCCCCCEEEECCCCCCHHHHHHHHCCCCCEEHHHHHHHCCCHHHHHHHHHHHHHCCCEE
YLAGPMDARESAHASTPVVGMPFWHQDGSHA
EEECCCHHHHHCCCCCCEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: deoxyxylulose-5-phosphate; ThiS-COSH; L-tyrosine [C]

Specific reaction: deoxyxylulose-5-phosphate + ThiS-COSH + L-tyrosine = 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate + 4-hydroxy-benzyl-alcohol + C1 of tyrosine + ThiS protein [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA