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The map label for this gene is thiE [H]

Identifier: 53720758

GI number: 53720758

Start: 3749822

End: 3750916

Strand: Reverse

Name: thiE [H]

Synonym: BPSL3151

Alternate gene names: 53720758

Gene position: 3750916-3749822 (Counterclockwise)

Preceding gene: 53720759

Following gene: 53720757

Centisome position: 92.06

GC content: 74.52

Gene sequence:

>1095_bases
ATGAGCGCCGCGTTGCCCGACGCGTTCTGGCCGCCCGCCGACGAGCTTACCGAGGCCGCCGAGCGGATTCGCGCGACGCT
CGGTGCGTGGCAGCGGCCGGCCGTGCGCACGCGGATCTGTCTCGCGCCGCCGGAGCAGCCGCGCGCGGCCGACCTGTGGG
TCGCCATCGCCGGCGCGCACGCCGCGCACATCGCGCGGCTGAACGCGGCGGGCGCGCAGGCGATCGTCATCGACGATGCA
TCGGCGACGCTCCACACGGGCGCGGCGCGCCATGCGCTCGCGTCGCGCGCGCCGCTCGCCGACGACTGGATCGCGGCGCT
CGCGGCGTTTCTCGATTGCGGCTTCGCCGCGTCCGACGCGCTCGTGCTCGCGCTCGCATGGCGCGACGGCGACGAGGCGC
GCGGCGGCGATCCGTGGCCCGTCGATCCGGCACGCTTTCCGCGCGTGCTCGGCCTGCCCGCCGCGCCCGAACCGGCGTTT
GCGCCGTGCCCGCAGCGGCTCGGCCTGTATCCGGTGCTGCCGAGCGCCGAATGGGTCGAGCGCGTGCTCGATTGCGGCGT
GCGGACCGTGCAACTGCGCGTGAAGGACGCCTCGCCCGACGCGCTGCGCGCGGAGATCGAGCGGGCCGTTGCCGCGGGCC
GCCGCCATCCGGACGCGCGCGTGTTCATCAACGATCACTGGCGGCTCGCGCTCGACGCGGGCGCATACGGCGTGCACCTC
GGCCAGGAGGATCTGGAGACCGCCGATCTCGGCGCGATCGCGCGGGCGGGCGCGCGGCTCGGCCTGTCGAGCCACGGGTA
TTACGAAATGCTCGTCGCGCTGCAGTTCAAGCCGAGCTATCTCGCGCTCGGCCCGGTGTTCGCGACCGCGACGAAGGCGG
TTGCCGCGCCGCCGCAAGGCCTCGCGCGGCTTGCGCGCTACGTGCGCTTCGCCGGGCCGCAGGCGCCGCTCGTCGCGATC
GGCGGAATCGCGCCCGACACGCTCGGCGCGGTGCTGGCGGCGGGCGTCGGCAGCGCGGCCGTCGTCAGCGCGATCACGGC
GGCGGCCGATTACCAGGAAGCGATTGTTGCATTGCAGCAAAACTTCGGACGATAA

Upstream 100 bases:

>100_bases
GCGCCGCGTATCTCGCGGGGCCGATGGATGCGCGCGAGAGCGCGCACGCGAGCACGCCCGTCGTCGGCATGCCGTTCTGG
CACCAGGACGGGAGCCACGC

Downstream 100 bases:

>100_bases
TTTGACAATTTCTGACTGGAGCGCCTGAGCGGCCTTTGCGGCATCATTCCGATGCAGGCCCTATAATTCGGCGTTCTGCG
TATAAGGATTGTCCGCTCCG

Product: thiamine-phosphate pyrophosphorylase

Products: NA

Alternate protein names: TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase [H]

Number of amino acids: Translated: 364; Mature: 363

Protein sequence:

>364_residues
MSAALPDAFWPPADELTEAAERIRATLGAWQRPAVRTRICLAPPEQPRAADLWVAIAGAHAAHIARLNAAGAQAIVIDDA
SATLHTGAARHALASRAPLADDWIAALAAFLDCGFAASDALVLALAWRDGDEARGGDPWPVDPARFPRVLGLPAAPEPAF
APCPQRLGLYPVLPSAEWVERVLDCGVRTVQLRVKDASPDALRAEIERAVAAGRRHPDARVFINDHWRLALDAGAYGVHL
GQEDLETADLGAIARAGARLGLSSHGYYEMLVALQFKPSYLALGPVFATATKAVAAPPQGLARLARYVRFAGPQAPLVAI
GGIAPDTLGAVLAAGVGSAAVVSAITAAADYQEAIVALQQNFGR

Sequences:

>Translated_364_residues
MSAALPDAFWPPADELTEAAERIRATLGAWQRPAVRTRICLAPPEQPRAADLWVAIAGAHAAHIARLNAAGAQAIVIDDA
SATLHTGAARHALASRAPLADDWIAALAAFLDCGFAASDALVLALAWRDGDEARGGDPWPVDPARFPRVLGLPAAPEPAF
APCPQRLGLYPVLPSAEWVERVLDCGVRTVQLRVKDASPDALRAEIERAVAAGRRHPDARVFINDHWRLALDAGAYGVHL
GQEDLETADLGAIARAGARLGLSSHGYYEMLVALQFKPSYLALGPVFATATKAVAAPPQGLARLARYVRFAGPQAPLVAI
GGIAPDTLGAVLAAGVGSAAVVSAITAAADYQEAIVALQQNFGR
>Mature_363_residues
SAALPDAFWPPADELTEAAERIRATLGAWQRPAVRTRICLAPPEQPRAADLWVAIAGAHAAHIARLNAAGAQAIVIDDAS
ATLHTGAARHALASRAPLADDWIAALAAFLDCGFAASDALVLALAWRDGDEARGGDPWPVDPARFPRVLGLPAAPEPAFA
PCPQRLGLYPVLPSAEWVERVLDCGVRTVQLRVKDASPDALRAEIERAVAAGRRHPDARVFINDHWRLALDAGAYGVHLG
QEDLETADLGAIARAGARLGLSSHGYYEMLVALQFKPSYLALGPVFATATKAVAAPPQGLARLARYVRFAGPQAPLVAIG
GIAPDTLGAVLAAGVGSAAVVSAITAAADYQEAIVALQQNFGR

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP) [H]

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family [H]

Homologues:

Organism=Escherichia coli, GI1790426, Length=208, Percent_Identity=55.2884615384615, Blast_Score=203, Evalue=1e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733 [H]

Pfam domain/function: PF02581 TMP-TENI [H]

EC number: =2.5.1.3 [H]

Molecular weight: Translated: 38047; Mature: 37915

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAALPDAFWPPADELTEAAERIRATLGAWQRPAVRTRICLAPPEQPRAADLWVAIAGAH
CCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHEEEEECCCCCCCCCEEEEEEECCH
AAHIARLNAAGAQAIVIDDASATLHTGAARHALASRAPLADDWIAALAAFLDCGFAASDA
HHHHHHHCCCCCEEEEEECCCCHHHCCHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCE
LVLALAWRDGDEARGGDPWPVDPARFPRVLGLPAAPEPAFAPCPQRLGLYPVLPSAEWVE
EEEEEEECCCCCCCCCCCCCCCHHHCCCEECCCCCCCCCCCCCHHHCCCEECCCCHHHHH
RVLDCGVRTVQLRVKDASPDALRAEIERAVAAGRRHPDARVFINDHWRLALDAGAYGVHL
HHHHCCCEEEEEEEECCCCHHHHHHHHHHHHHCCCCCCCEEEECCCEEEEEECCCCEEEC
GQEDLETADLGAIARAGARLGLSSHGYYEMLVALQFKPSYLALGPVFATATKAVAAPPQG
CHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEECCCEEEHHHHHHHHHHHHCCCHHH
LARLARYVRFAGPQAPLVAIGGIAPDTLGAVLAAGVGSAAVVSAITAAADYQEAIVALQQ
HHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
NFGR
HCCC
>Mature Secondary Structure 
SAALPDAFWPPADELTEAAERIRATLGAWQRPAVRTRICLAPPEQPRAADLWVAIAGAH
CCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHEEEEECCCCCCCCCEEEEEEECCH
AAHIARLNAAGAQAIVIDDASATLHTGAARHALASRAPLADDWIAALAAFLDCGFAASDA
HHHHHHHCCCCCEEEEEECCCCHHHCCHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCE
LVLALAWRDGDEARGGDPWPVDPARFPRVLGLPAAPEPAFAPCPQRLGLYPVLPSAEWVE
EEEEEEECCCCCCCCCCCCCCCHHHCCCEECCCCCCCCCCCCCHHHCCCEECCCCHHHHH
RVLDCGVRTVQLRVKDASPDALRAEIERAVAAGRRHPDARVFINDHWRLALDAGAYGVHL
HHHHCCCEEEEEEEECCCCHHHHHHHHHHHHHCCCCCCCEEEECCCEEEEEECCCCEEEC
GQEDLETADLGAIARAGARLGLSSHGYYEMLVALQFKPSYLALGPVFATATKAVAAPPQG
CHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEECCCEEEHHHHHHHHHHHHCCCHHH
LARLARYVRFAGPQAPLVAIGGIAPDTLGAVLAAGVGSAAVVSAITAAADYQEAIVALQQ
HHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
NFGR
HCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA