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The map label for this gene is citH [H]

Identifier: 53720481

GI number: 53720481

Start: 3438714

End: 3439337

Strand: Reverse

Name: citH [H]

Synonym: BPSL2873

Alternate gene names: 53720481

Gene position: 3439337-3438714 (Counterclockwise)

Preceding gene: 53720482

Following gene: 53720475

Centisome position: 84.41

GC content: 68.11

Gene sequence:

>624_bases
TTGGCGTGCGTGGCGCATGCCTATAATCGTGCGCGTTCGTCTTCGTCCGCCTTCGCCGTTCGAGGCGGCGCGGCGATCGG
CGCGGTCGTGCTCGGCACCGTCGCGACTTACCTCGTGCTGTTCATGCCGACCTACGGCGTGAAGGAGTTGAAGCTCGCGC
CGTCCGCGGCGTTCGCCGCGATTCTCGTCGTCGGCGTGATCCAGATGGGTTTCGCGCCGGTGGTCGGGCACTGGTCCGAT
CGCCACGGCCGCGTGCGGACGATGTGCGTGCCCGCGCTCGCGATCCTCGTGCTGATCTACCCGGCGTTCGCGTGGCTCGC
CGCGCATCCGACGTTCGGCGCGCTGATCGCCGTGCAGATCGTGTTCGCGTTCCTGATGACCGGCTATTTCGCCGCGCTGC
CGGGCCTGCTTTCGGAGATCTTTCCGGTTGCGACGCGCACGACCGGCATGTCGCTCGCGTATAACGTCGCGGTGACGGTC
TTCGGCGGATTCGGCCCGTTCATCATCGCGTGGCTGATCCGTGCGACGGGCACGAAAGCCGCGCCCAGCTTCTATCTGAT
CTTCGCCGCGCTCGCGAGCCTCGCGGCGCTCGTCGCGCTGCGGCGGCGCTTCGGGTTGCGCTGA

Upstream 100 bases:

>100_bases
GCGTTTTCGCGAGGCCGCCGCGTGCCGCGTGCGATGCGCGGCCGCCGCGTCGCGCCGACGAACCGCCGCGTTCCATATCG
GGCGCGGCGGTTTTCTTTTT

Downstream 100 bases:

>100_bases
CGCGCGGCGTTCGGCGCGCCCCGCATCCGGCGACCCAAGCCTCGTCGGCTCAAACGTCGCCGACTTGGGCCTCTGCGCCA
CGGCCGGCGGAGCCGGAACG

Product: hypothetical protein

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Citrate carrier protein; Citrate transporter [H]

Number of amino acids: Translated: 207; Mature: 206

Protein sequence:

>207_residues
MACVAHAYNRARSSSSAFAVRGGAAIGAVVLGTVATYLVLFMPTYGVKELKLAPSAAFAAILVVGVIQMGFAPVVGHWSD
RHGRVRTMCVPALAILVLIYPAFAWLAAHPTFGALIAVQIVFAFLMTGYFAALPGLLSEIFPVATRTTGMSLAYNVAVTV
FGGFGPFIIAWLIRATGTKAAPSFYLIFAALASLAALVALRRRFGLR

Sequences:

>Translated_207_residues
MACVAHAYNRARSSSSAFAVRGGAAIGAVVLGTVATYLVLFMPTYGVKELKLAPSAAFAAILVVGVIQMGFAPVVGHWSD
RHGRVRTMCVPALAILVLIYPAFAWLAAHPTFGALIAVQIVFAFLMTGYFAALPGLLSEIFPVATRTTGMSLAYNVAVTV
FGGFGPFIIAWLIRATGTKAAPSFYLIFAALASLAALVALRRRFGLR
>Mature_206_residues
ACVAHAYNRARSSSSAFAVRGGAAIGAVVLGTVATYLVLFMPTYGVKELKLAPSAAFAAILVVGVIQMGFAPVVGHWSDR
HGRVRTMCVPALAILVLIYPAFAWLAAHPTFGALIAVQIVFAFLMTGYFAALPGLLSEIFPVATRTTGMSLAYNVAVTVF
GGFGPFIIAWLIRATGTKAAPSFYLIFAALASLAALVALRRRFGLR

Specific function: Uptake of citrate across the boundary membrane with the concomitant transport of protons into the cell (symport system) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=178, Percent_Identity=26.4044943820225, Blast_Score=66, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 21799; Mature: 21668

Theoretical pI: Translated: 11.15; Mature: 11.15

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MACVAHAYNRARSSSSAFAVRGGAAIGAVVLGTVATYLVLFMPTYGVKELKLAPSAAFAA
CCHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCHHHHHH
ILVVGVIQMGFAPVVGHWSDRHGRVRTMCVPALAILVLIYPAFAWLAAHPTFGALIAVQI
HHHHHHHHHCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
VFAFLMTGYFAALPGLLSEIFPVATRTTGMSLAYNVAVTVFGGFGPFIIAWLIRATGTKA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHEECCHHHHHHHHHHHHCCCCC
APSFYLIFAALASLAALVALRRRFGLR
CHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
ACVAHAYNRARSSSSAFAVRGGAAIGAVVLGTVATYLVLFMPTYGVKELKLAPSAAFAA
CHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCHHHHHH
ILVVGVIQMGFAPVVGHWSDRHGRVRTMCVPALAILVLIYPAFAWLAAHPTFGALIAVQI
HHHHHHHHHCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
VFAFLMTGYFAALPGLLSEIFPVATRTTGMSLAYNVAVTVFGGFGPFIIAWLIRATGTKA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHEECCHHHHHHHHHHHHCCCCC
APSFYLIFAALASLAALVALRRRFGLR
CHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2186908 [H]