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The map label for this gene is truB

Identifier: 53719530

GI number: 53719530

Start: 2283918

End: 2284853

Strand: Reverse

Name: truB

Synonym: BPSL1916

Alternate gene names: 53719530

Gene position: 2284853-2283918 (Counterclockwise)

Preceding gene: 53719531

Following gene: 53719525

Centisome position: 56.08

GC content: 71.05

Gene sequence:

>936_bases
ATGACGACTGTTTCGCCCCGCCCCCGGATGGCCCGCCGCGCGCTCGATGGCGTGCTGCTGCTCGACAAGCCGGTCGGCCT
GTCGAGCAACGACGCGCTGATGCGCGCGAAACGCCTGTATCAGGCGAAGAAGGCGGGCCACACGGGCACGCTCGATCCGC
TCGCGTCGGGACTGCTGCCGCTTTGCTTCGGCGAAGCGACGAAGTTCTCGCAGGACCTGCTCGAAGCCGACAAGACCTAC
GAAGCGACGATGCGGCTCGGCGTGCGCACGACGACGGGCGACGCCGAAGGCGACGTGCTCGACACGCGCGACGTGAGCTG
CGACGAAGCGGCGGTCCGCGCGGCGCTCGCGCGCTTCGTCGGCGAGATCGTGCAAGTGCCGCCGATGTACTCGGCGCTCA
AGCGCGACGGCAAGCCGCTTTACGAATACGCGCGCGCCGGCCAGACGGTCGAGCGCGAAGGGCGCACGGTGACGATCCGC
GCGCTCGCGCTCGTGTCGTGCGCGTTGCCCGACGTCACGTTTCGCGTGACGTGCAGCAAGGGCACGTACGTGCGCACGCT
CGCCGAGGATATCGGCGAAGCGCTCGGTTGCGGCGCGCATCTGACGATGTTGCGGCGCACCGGGGTCGGCCCGCTGACGC
TCGAGCACGCGGTGACGCTCGATGCGCTCGACGCCGCGACGCAGGACGAGCGCGACGCGCGGCTCGCGCCCGTCGATGCA
TTGCTGTCGACGTTTCCGTGCGTGAAGCTCGATGCGGCGCTCGCGACGCGCTTTCTGCACGGCCAGCGGCTGAAGCTCTC
CGAGCTTGCGGCGCGGCCCGACGCGGCCGAAGGTGGGCGCGTGCGCGTCTACGATGCCGATGACCGGCTGCTCGGCGTCG
CGCGCGCGTCGGAAGGCGTGCTCGCGCCGGAGCGGCTCGTCGTGACGGGCGCGTGA

Upstream 100 bases:

>100_bases
AGACGATCGAGAAGGCCGTCGCGATGTCGCGCCTCATCGACGAGGCGAACGCGACGCGCGCGAAGGACGATTGAGCATCG
CACGAGCCTTGGTGCAGACC

Downstream 100 bases:

>100_bases
TCGCCCGAACGCGCGGGGCGGCGATTTGATCCGCGCTCGCGCGGCACGCGATGCGCCGGGCACGGCCGACGGTCGATGCG
GCGGTGATCGCCGATGGCGC

Product: tRNA pseudouridine synthase B

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase

Number of amino acids: Translated: 311; Mature: 310

Protein sequence:

>311_residues
MTTVSPRPRMARRALDGVLLLDKPVGLSSNDALMRAKRLYQAKKAGHTGTLDPLASGLLPLCFGEATKFSQDLLEADKTY
EATMRLGVRTTTGDAEGDVLDTRDVSCDEAAVRAALARFVGEIVQVPPMYSALKRDGKPLYEYARAGQTVEREGRTVTIR
ALALVSCALPDVTFRVTCSKGTYVRTLAEDIGEALGCGAHLTMLRRTGVGPLTLEHAVTLDALDAATQDERDARLAPVDA
LLSTFPCVKLDAALATRFLHGQRLKLSELAARPDAAEGGRVRVYDADDRLLGVARASEGVLAPERLVVTGA

Sequences:

>Translated_311_residues
MTTVSPRPRMARRALDGVLLLDKPVGLSSNDALMRAKRLYQAKKAGHTGTLDPLASGLLPLCFGEATKFSQDLLEADKTY
EATMRLGVRTTTGDAEGDVLDTRDVSCDEAAVRAALARFVGEIVQVPPMYSALKRDGKPLYEYARAGQTVEREGRTVTIR
ALALVSCALPDVTFRVTCSKGTYVRTLAEDIGEALGCGAHLTMLRRTGVGPLTLEHAVTLDALDAATQDERDARLAPVDA
LLSTFPCVKLDAALATRFLHGQRLKLSELAARPDAAEGGRVRVYDADDRLLGVARASEGVLAPERLVVTGA
>Mature_310_residues
TTVSPRPRMARRALDGVLLLDKPVGLSSNDALMRAKRLYQAKKAGHTGTLDPLASGLLPLCFGEATKFSQDLLEADKTYE
ATMRLGVRTTTGDAEGDVLDTRDVSCDEAAVRAALARFVGEIVQVPPMYSALKRDGKPLYEYARAGQTVEREGRTVTIRA
LALVSCALPDVTFRVTCSKGTYVRTLAEDIGEALGCGAHLTMLRRTGVGPLTLEHAVTLDALDAATQDERDARLAPVDAL
LSTFPCVKLDAALATRFLHGQRLKLSELAARPDAAEGGRVRVYDADDRLLGVARASEGVLAPERLVVTGA

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily

Homologues:

Organism=Homo sapiens, GI21040257, Length=251, Percent_Identity=35.4581673306773, Blast_Score=125, Evalue=6e-29,
Organism=Homo sapiens, GI4503337, Length=261, Percent_Identity=28.735632183908, Blast_Score=75, Evalue=5e-14,
Organism=Homo sapiens, GI215599015, Length=261, Percent_Identity=28.735632183908, Blast_Score=75, Evalue=6e-14,
Organism=Escherichia coli, GI2367200, Length=314, Percent_Identity=45.5414012738853, Blast_Score=255, Evalue=2e-69,
Organism=Caenorhabditis elegans, GI17553978, Length=287, Percent_Identity=28.5714285714286, Blast_Score=96, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6324037, Length=196, Percent_Identity=34.1836734693878, Blast_Score=106, Evalue=6e-24,
Organism=Saccharomyces cerevisiae, GI6323204, Length=261, Percent_Identity=29.8850574712644, Blast_Score=95, Evalue=2e-20,
Organism=Drosophila melanogaster, GI281364189, Length=270, Percent_Identity=26.6666666666667, Blast_Score=82, Evalue=7e-16,
Organism=Drosophila melanogaster, GI281364187, Length=270, Percent_Identity=26.6666666666667, Blast_Score=82, Evalue=7e-16,
Organism=Drosophila melanogaster, GI281364185, Length=270, Percent_Identity=26.6666666666667, Blast_Score=82, Evalue=7e-16,
Organism=Drosophila melanogaster, GI281364183, Length=270, Percent_Identity=26.6666666666667, Blast_Score=82, Evalue=7e-16,
Organism=Drosophila melanogaster, GI62471759, Length=270, Percent_Identity=26.6666666666667, Blast_Score=82, Evalue=7e-16,
Organism=Drosophila melanogaster, GI17975520, Length=270, Percent_Identity=26.6666666666667, Blast_Score=82, Evalue=7e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUB_BURMA (Q62KL1)

Other databases:

- EMBL:   CP000010
- RefSeq:   YP_102758.1
- ProteinModelPortal:   Q62KL1
- SMR:   Q62KL1
- GeneID:   3090885
- GenomeReviews:   CP000010_GR
- KEGG:   bma:BMA1059
- TIGR:   BMA1059
- HOGENOM:   HBG397258
- OMA:   LGCGAYV
- PhylomeDB:   Q62KL1
- ProtClustDB:   PRK01851
- BioCyc:   BMAL243160:BMA_1059-MONOMER
- HAMAP:   MF_01080
- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR015947
- InterPro:   IPR014780
- InterPro:   IPR015240
- TIGRFAMs:   TIGR00431

Pfam domain/function: PF09157 TruB-C_2; PF01509 TruB_N; SSF55120 PsdUridine_synth_cat_dom; SSF88697 PUA-like

EC number: 4.2.1.70

Molecular weight: Translated: 33304; Mature: 33172

Theoretical pI: Translated: 7.37; Mature: 7.37

Prosite motif: NA

Important sites: ACT_SITE 52-52

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTVSPRPRMARRALDGVLLLDKPVGLSSNDALMRAKRLYQAKKAGHTGTLDPLASGLLP
CCCCCCCHHHHHHHHCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHCCHH
LCFGEATKFSQDLLEADKTYEATMRLGVRTTTGDAEGDVLDTRDVSCDEAAVRAALARFV
HHHCCHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCEECCCCCCHHHHHHHHHHHHHH
GEIVQVPPMYSALKRDGKPLYEYARAGQTVEREGRTVTIRALALVSCALPDVTFRVTCSK
HHHHCCCCHHHHHHHCCCHHHHHHHCCCCCHHCCCEEEEHHHHHHHHCCCCEEEEEEECC
GTYVRTLAEDIGEALGCGAHLTMLRRTGVGPLTLEHAVTLDALDAATQDERDARLAPVDA
CCHHHHHHHHHHHHHCCCHHHHHHHHCCCCCEEHHHHHHHHHHHCCCCCCCCCCCCHHHH
LLSTFPCVKLDAALATRFLHGQRLKLSELAARPDAAEGGRVRVYDADDRLLGVARASEGV
HHHHCCCHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCCCEEEEEECCCCEEEEHHCCCCC
LAPERLVVTGA
CCCCCEEEECC
>Mature Secondary Structure 
TTVSPRPRMARRALDGVLLLDKPVGLSSNDALMRAKRLYQAKKAGHTGTLDPLASGLLP
CCCCCCHHHHHHHHCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHCCHH
LCFGEATKFSQDLLEADKTYEATMRLGVRTTTGDAEGDVLDTRDVSCDEAAVRAALARFV
HHHCCHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCEECCCCCCHHHHHHHHHHHHHH
GEIVQVPPMYSALKRDGKPLYEYARAGQTVEREGRTVTIRALALVSCALPDVTFRVTCSK
HHHHCCCCHHHHHHHCCCHHHHHHHCCCCCHHCCCEEEEHHHHHHHHCCCCEEEEEEECC
GTYVRTLAEDIGEALGCGAHLTMLRRTGVGPLTLEHAVTLDALDAATQDERDARLAPVDA
CCHHHHHHHHHHHHHCCCHHHHHHHHCCCCCEEHHHHHHHHHHHCCCCCCCCCCCCHHHH
LLSTFPCVKLDAALATRFLHGQRLKLSELAARPDAAEGGRVRVYDADDRLLGVARASEGV
HHHHCCCHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCCCEEEEEECCCCEEEEHHCCCCC
LAPERLVVTGA
CCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA