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The map label for this gene is gcl [H]

Identifier: 53719088

GI number: 53719088

Start: 1688070

End: 1689845

Strand: Reverse

Name: gcl [H]

Synonym: BPSL1452

Alternate gene names: 53719088

Gene position: 1689845-1688070 (Counterclockwise)

Preceding gene: 53719090

Following gene: 53719087

Centisome position: 41.47

GC content: 68.19

Gene sequence:

>1776_bases
ATGGCCAGGATGAGAGCCGTCGACGCAGCCGTGCTCGTGCTCGAGAAGGAAGGCGTGCAGACCGCGTTCGGCGTGCCGGG
CGCCGCGATCAACCCGCTGTATTCGGCGCTGCGCCGCTCGGGCGCGATCGGCCATGTGCTCGCGCGGCACGTCGAGGGCG
CGTCGCACATGGCGGAAGGCTACACGCGGGCCGCGCCCGGCAACATCGGCGTGTGCATCGGCACGTCGGGCCCGGCCGGC
ACCGACATGATCACGGGCCTGTACTCGGCGTCGGCCGATTCGATCCCGATCCTCGCGATCACCGGCCAGGCGCCGCGCGC
GCGGCTCCACAAGGAAGACTTCCAGGCGGTCGACATCGAATCGATCGCGAAGCCCGTCACGAAATGGGCGGTCACGGTGC
GCGAGCCGGCGCTCGTGCCGCGCGTGTTCCAGCAGGCGTTCCACCTGATGCGCTCGGGCCGCCCGGGCCCCGTGCTGATC
GATCTGCCGATCGACGTGCAACTCGCCGAGATCGAATTCGACATCGCGACCTACGAGCCGCTGCCCGTCTACAAGCCGCG
CGCGACGCGCGCGCAGATCGAGGCCGCGCTCGCGATGCTGAACGACGCCGAGCGCCCGCTCATCGTGTCGGGCGGCGGCG
TGATCAACGCGGCGGCCGAGGAACTGCTCGTGCAGTTCGCCGAAACGCTCGGCGTGCCGGTGATCCCGACGCTGATGTCG
TGGGGCGCGATTGCCGACGATCATCCGCTGATGGCCGGCATGGTCGGCCTGCAGACGTCGCACCGCTACGGCAACGCGAC
GCTGCTCGCCTCCGACTTCGTGCTCGGCATCGGCAACCGCTGGGCCAATCGCCACACGGGCAGCATCGAGGTCTACACGA
AGGGCCGCAAGTTCGTGCACGTCGACATCGAACCGACGCAGATCGGCCGCGTGTTCGGCCCCGATCTCGGGATCGTGTCG
GACGCGAAGGCGGCGCTCGAGCTGTTCGTCGAGATCGCGCGCGAATGGAAGGCCGCGGGCAAGCTGAAGAACCGCGGCGC
GTGGGTCGCCGACTGTCAGCAGCGCAAGCGCACGCTGCAGCGCAAGACGCATTTCGACGACGTGCCCATCAAGCCGCAGC
GCGTGTACGAAGAGATGAACAAGGCATTCGGCCGCGATACGTGCTACGTGAGCACGATCGGGCTGTCGCAGATCGCGGCC
GCGCAGTTCCTGCACGTGTTCAAGGCGCGCAACTGGATCAACTGCGGCCAGGCGGGCCCGCTCGGCTGGACGATTCCCGC
CGCGCTCGGCGTGCGCGCGGCCGATCCGCAGCGCCCGATCGTCGCGCTGTCGGGCGACTACGACTTCCAGTTCATGATCG
AGGAGCTCGCCGCGGGCGCGCAGTTCAAGCTGCCCTATGTGCACGTCGTCGTCAACAACGCCTATCTCGGCCTGATCCGC
CAGGCACAGCGCGCGTTCGACATGGACTATTGCGTGCAGCTCGCGTTCGACAACGTCAACGCGCCCGAGATGAACGGCTA
TGGCGTCGATCATGTCGCCGTCGCCGAAGGCCTCGGCTGCAAGGCGCTGCGCGTGTTCAAGCCCGAGGAGATCGCGCCGG
CGCTCGAACGCGCGCGGCAGATGACGGCCGAGTTCAGCGTGCCCGTCGTCGTCGAGGTGATTCTCGAGCGGGTGACCAAC
ATATCGATGGGCGCCGAGATCGACGCGATCAACGAGTTCGAGGATCTCGCGGACAAGGCCGAGCACGCGCCCACCGCGAT
CTCGCTGCTCGACTGA

Upstream 100 bases:

>100_bases
AATAAGCGACTCGACAGGATCTTATCAAACCTTTCCTGCATCCCTACAGTGTCCTCGACACCGCGATACCCGCGATTCGC
ATCGAACAAGGAGACATTTC

Downstream 100 bases:

>100_bases
CGCGCGCCGCGCCTATCGCACCGCCCGCCCGGTCGATCAGTTCGACTGATCGATTCGGCCGGCTCGCTCACCTGGCTTCA
TCCGACCTCAACTGACCGAC

Product: glyoxylate carboligase

Products: NA

Alternate protein names: Tartronate-semialdehyde synthase [H]

Number of amino acids: Translated: 591; Mature: 590

Protein sequence:

>591_residues
MARMRAVDAAVLVLEKEGVQTAFGVPGAAINPLYSALRRSGAIGHVLARHVEGASHMAEGYTRAAPGNIGVCIGTSGPAG
TDMITGLYSASADSIPILAITGQAPRARLHKEDFQAVDIESIAKPVTKWAVTVREPALVPRVFQQAFHLMRSGRPGPVLI
DLPIDVQLAEIEFDIATYEPLPVYKPRATRAQIEAALAMLNDAERPLIVSGGGVINAAAEELLVQFAETLGVPVIPTLMS
WGAIADDHPLMAGMVGLQTSHRYGNATLLASDFVLGIGNRWANRHTGSIEVYTKGRKFVHVDIEPTQIGRVFGPDLGIVS
DAKAALELFVEIAREWKAAGKLKNRGAWVADCQQRKRTLQRKTHFDDVPIKPQRVYEEMNKAFGRDTCYVSTIGLSQIAA
AQFLHVFKARNWINCGQAGPLGWTIPAALGVRAADPQRPIVALSGDYDFQFMIEELAAGAQFKLPYVHVVVNNAYLGLIR
QAQRAFDMDYCVQLAFDNVNAPEMNGYGVDHVAVAEGLGCKALRVFKPEEIAPALERARQMTAEFSVPVVVEVILERVTN
ISMGAEIDAINEFEDLADKAEHAPTAISLLD

Sequences:

>Translated_591_residues
MARMRAVDAAVLVLEKEGVQTAFGVPGAAINPLYSALRRSGAIGHVLARHVEGASHMAEGYTRAAPGNIGVCIGTSGPAG
TDMITGLYSASADSIPILAITGQAPRARLHKEDFQAVDIESIAKPVTKWAVTVREPALVPRVFQQAFHLMRSGRPGPVLI
DLPIDVQLAEIEFDIATYEPLPVYKPRATRAQIEAALAMLNDAERPLIVSGGGVINAAAEELLVQFAETLGVPVIPTLMS
WGAIADDHPLMAGMVGLQTSHRYGNATLLASDFVLGIGNRWANRHTGSIEVYTKGRKFVHVDIEPTQIGRVFGPDLGIVS
DAKAALELFVEIAREWKAAGKLKNRGAWVADCQQRKRTLQRKTHFDDVPIKPQRVYEEMNKAFGRDTCYVSTIGLSQIAA
AQFLHVFKARNWINCGQAGPLGWTIPAALGVRAADPQRPIVALSGDYDFQFMIEELAAGAQFKLPYVHVVVNNAYLGLIR
QAQRAFDMDYCVQLAFDNVNAPEMNGYGVDHVAVAEGLGCKALRVFKPEEIAPALERARQMTAEFSVPVVVEVILERVTN
ISMGAEIDAINEFEDLADKAEHAPTAISLLD
>Mature_590_residues
ARMRAVDAAVLVLEKEGVQTAFGVPGAAINPLYSALRRSGAIGHVLARHVEGASHMAEGYTRAAPGNIGVCIGTSGPAGT
DMITGLYSASADSIPILAITGQAPRARLHKEDFQAVDIESIAKPVTKWAVTVREPALVPRVFQQAFHLMRSGRPGPVLID
LPIDVQLAEIEFDIATYEPLPVYKPRATRAQIEAALAMLNDAERPLIVSGGGVINAAAEELLVQFAETLGVPVIPTLMSW
GAIADDHPLMAGMVGLQTSHRYGNATLLASDFVLGIGNRWANRHTGSIEVYTKGRKFVHVDIEPTQIGRVFGPDLGIVSD
AKAALELFVEIAREWKAAGKLKNRGAWVADCQQRKRTLQRKTHFDDVPIKPQRVYEEMNKAFGRDTCYVSTIGLSQIAAA
QFLHVFKARNWINCGQAGPLGWTIPAALGVRAADPQRPIVALSGDYDFQFMIEELAAGAQFKLPYVHVVVNNAYLGLIRQ
AQRAFDMDYCVQLAFDNVNAPEMNGYGVDHVAVAEGLGCKALRVFKPEEIAPALERARQMTAEFSVPVVVEVILERVTNI
SMGAEIDAINEFEDLADKAEHAPTAISLLD

Specific function: Catalyzes the condensation of two molecules of glyoxylate to give 2-hydroxy-3-oxopropanoate (also termed tartronate semialdehyde) [H]

COG id: COG3960

COG function: function code R; Glyoxylate carboligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=562, Percent_Identity=23.8434163701068, Blast_Score=130, Evalue=5e-30,
Organism=Homo sapiens, GI21361361, Length=597, Percent_Identity=24.1206030150754, Blast_Score=115, Evalue=2e-25,
Organism=Escherichia coli, GI1786717, Length=590, Percent_Identity=78.9830508474576, Blast_Score=980, Evalue=0.0,
Organism=Escherichia coli, GI87081685, Length=544, Percent_Identity=34.0073529411765, Blast_Score=305, Evalue=4e-84,
Organism=Escherichia coli, GI1790104, Length=491, Percent_Identity=33.8085539714868, Blast_Score=263, Evalue=2e-71,
Organism=Escherichia coli, GI1787096, Length=551, Percent_Identity=26.3157894736842, Blast_Score=168, Evalue=1e-42,
Organism=Escherichia coli, GI1788716, Length=476, Percent_Identity=25.4201680672269, Blast_Score=133, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI17542570, Length=553, Percent_Identity=24.9547920433996, Blast_Score=109, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI17531301, Length=543, Percent_Identity=22.8360957642726, Blast_Score=100, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI17531299, Length=543, Percent_Identity=22.8360957642726, Blast_Score=99, Evalue=6e-21,
Organism=Saccharomyces cerevisiae, GI6323755, Length=601, Percent_Identity=29.783693843594, Blast_Score=255, Evalue=1e-68,
Organism=Saccharomyces cerevisiae, GI6320816, Length=564, Percent_Identity=24.468085106383, Blast_Score=109, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6321524, Length=492, Percent_Identity=20.9349593495935, Blast_Score=71, Evalue=6e-13,
Organism=Saccharomyces cerevisiae, GI6323073, Length=514, Percent_Identity=22.568093385214, Blast_Score=64, Evalue=9e-11,
Organism=Drosophila melanogaster, GI19922626, Length=572, Percent_Identity=26.048951048951, Blast_Score=149, Evalue=4e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006397
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =4.1.1.47 [H]

Molecular weight: Translated: 64008; Mature: 63877

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARMRAVDAAVLVLEKEGVQTAFGVPGAAINPLYSALRRSGAIGHVLARHVEGASHMAEG
CCCCHHHCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHC
YTRAAPGNIGVCIGTSGPAGTDMITGLYSASADSIPILAITGQAPRARLHKEDFQAVDIE
CCCCCCCCEEEEEECCCCCCHHHHHHHHHCCCCCCEEEEEECCCCHHHHCHHCCCEECHH
SIAKPVTKWAVTVREPALVPRVFQQAFHLMRSGRPGPVLIDLPIDVQLAEIEFDIATYEP
HHHHHHHHHEEEECCCHHHHHHHHHHHHHHHCCCCCCEEEECCCCEEEEEEEEEEECCCC
LPVYKPRATRAQIEAALAMLNDAERPLIVSGGGVINAAAEELLVQFAETLGVPVIPTLMS
CCCCCCCCHHHHHHHHHHHHCCCCCCEEEECCCEEHHHHHHHHHHHHHHCCCCHHHHHHH
WGAIADDHPLMAGMVGLQTSHRYGNATLLASDFVLGIGNRWANRHTGSIEVYTKGRKFVH
CCCCCCCCCHHHHHHHHCCCCCCCCEEEEECCCEEECCCCCCCCCCCEEEEEECCCEEEE
VDIEPTQIGRVFGPDLGIVSDAKAALELFVEIAREWKAAGKLKNRGAWVADCQQRKRTLQ
EECCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
RKTHFDDVPIKPQRVYEEMNKAFGRDTCYVSTIGLSQIAAAQFLHVFKARNWINCGQAGP
HHCCCCCCCCCHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHCCCCCCCCCCC
LGWTIPAALGVRAADPQRPIVALSGDYDFQFMIEELAAGAQFKLPYVHVVVNNAYLGLIR
CCEEHHHHHCCCCCCCCCCEEEEECCCCHHHHHHHHCCCCEECCCEEEEEECCHHHHHHH
QAQRAFDMDYCVQLAFDNVNAPEMNGYGVDHVAVAEGLGCKALRVFKPEEIAPALERARQ
HHHHHHCHHHHHHEEECCCCCCCCCCCCCCHHHHHCCCCCCEEEECCCHHHHHHHHHHHH
MTAEFSVPVVVEVILERVTNISMGAEIDAINEFEDLADKAEHAPTAISLLD
HHHHCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECC
>Mature Secondary Structure 
ARMRAVDAAVLVLEKEGVQTAFGVPGAAINPLYSALRRSGAIGHVLARHVEGASHMAEG
CCCHHHCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHC
YTRAAPGNIGVCIGTSGPAGTDMITGLYSASADSIPILAITGQAPRARLHKEDFQAVDIE
CCCCCCCCEEEEEECCCCCCHHHHHHHHHCCCCCCEEEEEECCCCHHHHCHHCCCEECHH
SIAKPVTKWAVTVREPALVPRVFQQAFHLMRSGRPGPVLIDLPIDVQLAEIEFDIATYEP
HHHHHHHHHEEEECCCHHHHHHHHHHHHHHHCCCCCCEEEECCCCEEEEEEEEEEECCCC
LPVYKPRATRAQIEAALAMLNDAERPLIVSGGGVINAAAEELLVQFAETLGVPVIPTLMS
CCCCCCCCHHHHHHHHHHHHCCCCCCEEEECCCEEHHHHHHHHHHHHHHCCCCHHHHHHH
WGAIADDHPLMAGMVGLQTSHRYGNATLLASDFVLGIGNRWANRHTGSIEVYTKGRKFVH
CCCCCCCCCHHHHHHHHCCCCCCCCEEEEECCCEEECCCCCCCCCCCEEEEEECCCEEEE
VDIEPTQIGRVFGPDLGIVSDAKAALELFVEIAREWKAAGKLKNRGAWVADCQQRKRTLQ
EECCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
RKTHFDDVPIKPQRVYEEMNKAFGRDTCYVSTIGLSQIAAAQFLHVFKARNWINCGQAGP
HHCCCCCCCCCHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHCCCCCCCCCCC
LGWTIPAALGVRAADPQRPIVALSGDYDFQFMIEELAAGAQFKLPYVHVVVNNAYLGLIR
CCEEHHHHHCCCCCCCCCCEEEEECCCCHHHHHHHHCCCCEECCCEEEEEECCHHHHHHH
QAQRAFDMDYCVQLAFDNVNAPEMNGYGVDHVAVAEGLGCKALRVFKPEEIAPALERARQ
HHHHHHCHHHHHHEEECCCCCCCCCCCCCCHHHHHCCCCCCEEEECCCHHHHHHHHHHHH
MTAEFSVPVVVEVILERVTNISMGAEIDAINEFEDLADKAEHAPTAISLLD
HHHHCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]