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The map label for this gene is rnhA [H]

Identifier: 53718981

GI number: 53718981

Start: 1567199

End: 1567645

Strand: Reverse

Name: rnhA [H]

Synonym: BPSL1342

Alternate gene names: 53718981

Gene position: 1567645-1567199 (Counterclockwise)

Preceding gene: 53718982

Following gene: 53718980

Centisome position: 38.47

GC content: 66.67

Gene sequence:

>447_bases
ATGACGTTGCAAACCATCGACATCTATACCGACGGCGCCTGCAAGGGCAACCCCGGCCCGGGCGGCTGGGGCGCGCTGCT
GCGCTACGGCGCGCAGGAGAAAGAGCTTTTCGGCGGCGAACCCGGCACGACCAACAACCGGATGGAGCTCACCGCGGTGA
TCGCCGCGCTCGAGGCGCTCAAGCGCCCGTGCAAGGTGGTCGTCCACACCGATTCGCAATACGTGCAAAAAGGCATCAGC
GAATGGATCCACGGCTGGAAGAAGAAAGGCTGGGTCACCGCCGCGAAAACGCCCGTGAAGAACGCGGACCTCTGGCAGCG
GCTCGATGCGCTCGTCGCGCAGCACGACGTCGAATGGCGCTGGGTGAAGGGGCACGCCGGCCATCCGGAGAACGAGCGCG
CGGACGCGCTCGCGAACCGCGGCGTCGAATCGCTCGCGCAGGCATGA

Upstream 100 bases:

>100_bases
TGCGCGGGATGCGGCTCGTCGGCCCGATCAAGATGAAGAAGCCGGTGCTCGCGCCGGGCCTCACGCCCGCCGCCACCCCG
ACCACTCACCAAGAACATTC

Downstream 100 bases:

>100_bases
GCCCGCTCGCGCCCCCCTTTGCCCTTCACGACTGACTCGAATTTCCGTTTTCCCGACATGCGCCAGATCATTCTCGATAC
CGAAACCACCGGCCTGAACG

Product: ribonuclease HI

Products: NA

Alternate protein names: RNase H [H]

Number of amino acids: Translated: 148; Mature: 147

Protein sequence:

>148_residues
MTLQTIDIYTDGACKGNPGPGGWGALLRYGAQEKELFGGEPGTTNNRMELTAVIAALEALKRPCKVVVHTDSQYVQKGIS
EWIHGWKKKGWVTAAKTPVKNADLWQRLDALVAQHDVEWRWVKGHAGHPENERADALANRGVESLAQA

Sequences:

>Translated_148_residues
MTLQTIDIYTDGACKGNPGPGGWGALLRYGAQEKELFGGEPGTTNNRMELTAVIAALEALKRPCKVVVHTDSQYVQKGIS
EWIHGWKKKGWVTAAKTPVKNADLWQRLDALVAQHDVEWRWVKGHAGHPENERADALANRGVESLAQA
>Mature_147_residues
TLQTIDIYTDGACKGNPGPGGWGALLRYGAQEKELFGGEPGTTNNRMELTAVIAALEALKRPCKVVVHTDSQYVQKGISE
WIHGWKKKGWVTAAKTPVKNADLWQRLDALVAQHDVEWRWVKGHAGHPENERADALANRGVESLAQA

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids [H]

COG id: COG0328

COG function: function code L; Ribonuclease HI

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 RNase H domain [H]

Homologues:

Organism=Homo sapiens, GI21359816, Length=150, Percent_Identity=36.6666666666667, Blast_Score=79, Evalue=2e-15,
Organism=Escherichia coli, GI1786408, Length=136, Percent_Identity=65.4411764705882, Blast_Score=198, Evalue=1e-52,
Organism=Caenorhabditis elegans, GI115532542, Length=143, Percent_Identity=39.8601398601399, Blast_Score=98, Evalue=2e-21,
Organism=Drosophila melanogaster, GI45552509, Length=143, Percent_Identity=35.6643356643357, Blast_Score=81, Evalue=2e-16,
Organism=Drosophila melanogaster, GI17137476, Length=141, Percent_Identity=36.1702127659575, Blast_Score=81, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022892
- InterPro:   IPR012337
- InterPro:   IPR002156 [H]

Pfam domain/function: PF00075 RnaseH [H]

EC number: =3.1.26.4 [H]

Molecular weight: Translated: 16255; Mature: 16123

Theoretical pI: Translated: 8.24; Mature: 8.24

Prosite motif: PS50879 RNASE_H

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLQTIDIYTDGACKGNPGPGGWGALLRYGAQEKELFGGEPGTTNNRMELTAVIAALEAL
CCCEEEEEEECCCCCCCCCCCHHHHHHHCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHH
KRPCKVVVHTDSQYVQKGISEWIHGWKKKGWVTAAKTPVKNADLWQRLDALVAQHDVEWR
CCCCEEEEECCHHHHHHHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHHHHHCCCCEE
WVKGHAGHPENERADALANRGVESLAQA
EEECCCCCCCCHHHHHHHHCCHHHHHCC
>Mature Secondary Structure 
TLQTIDIYTDGACKGNPGPGGWGALLRYGAQEKELFGGEPGTTNNRMELTAVIAALEAL
CCEEEEEEECCCCCCCCCCCHHHHHHHCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHH
KRPCKVVVHTDSQYVQKGISEWIHGWKKKGWVTAAKTPVKNADLWQRLDALVAQHDVEWR
CCCCEEEEECCHHHHHHHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHHHHHCCCCEE
WVKGHAGHPENERADALANRGVESLAQA
EEECCCCCCCCHHHHHHHHCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14500782 [H]