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The map label for this gene is metG [H]

Identifier: 53718640

GI number: 53718640

Start: 1159815

End: 1161992

Strand: Reverse

Name: metG [H]

Synonym: BPSL0998

Alternate gene names: 53718640

Gene position: 1161992-1159815 (Counterclockwise)

Preceding gene: 53718641

Following gene: 53718635

Centisome position: 28.52

GC content: 67.08

Gene sequence:

>2178_bases
ATGTCCGCATCCGACCTCACTTCCGTGCAGGCCGGCGCGCCGCAAGGCCGCCGCCAGATCCTCGTCACGTCCGCGCTGCC
CTATGCGAACGGGCAGATTCACATCGGCCATCTGGTCGAGTACATCCAGACCGACATCTGGGTGCGGACGATGCGAATGC
ACGGTCACGAGATCTACTACATCGGCGCGGACGACACGCACGGCACGCCCGTCATGCTGCGCGCCGAGCAGGAAGGCGTG
AGCCCGAAGCAGTTGATCGAGCGCGTGTGGCGCGAGCACAAGCGCGATTTCGACAGCTTCGGCGTGTCGTTCGACAACTT
CTACACGACCGATTCGGACGAGAACCGCGTGCTCAGCGAGACGATCTATCTCGCGCTGAAGGAGGCGGGCTTCATCGCCG
AGCGCGAGATCGAGCAGGCGTACGACCCCGTCAGGCAAATGTTCCTGCCGGACCGCTTCATCAAGGGCGAATGCCCGAAG
TGCCACGCGAAGGACCAGTACGGCGACAGCTGCGAGGTGTGCGGCACGACGTACCAGCCGACGGACCTCATCCATCCGTA
CTCGGTCGTCTCGGGCGCCGCGCCCGTGCGCAAGACGTCGACGCACTACTTCTTCCGCCTGTCCGATCCGCGCTGCGAGG
CGTTCCTGCGCGAATGGGTGAGCGGCCTCGCGCAGCCCGAGGCGACCAACAAGATGCGCGAATGGCTCGGCGAGGCGGGC
GAGGCGAAGCTCGCCGACTGGGACATCTCGCGCGACGCGCCGTACTTCGGCTTCGAGATTCCCGGCGCGCCCGGCAAGTA
TTTCTACGTATGGCTCGACGCGCCCGTCGGCTACTACGCGAGCTTCAAGAACCTGTGCCAGCGGCGCGGCCTCGATTTCG
ACGCGTGGATCCGCAAGGATTCGACGACCGAGCAGTACCACTTCATCGGCAAGGACATCCTGTACTTCCATACGCTGTTC
TGGCCCGCGATGCTCGAGTTCTCGGGCCACCGCACGCCGACCAACGTGTTCGCGCACGGCTTCCTGACGGTCGACGGCGC
GAAGATGTCGAAATCGCGCGGCACGTTCATCACCGCGCAGAGCTACATCGACACGGGCCTGAACCCCGAATGGCTGCGCT
ACTACTTCGCCGCGAAGCTGAACGCGACGATGGAAGACATCGACCTGAACCTCGAGGATTTCCAGGCGCGCGTCAATAGC
GACCTCGTCGGCAAGTACGTGAACATCGCGAGCCGCGCGGCGGGCTTCCTGCTCAAGCGCTTCGACGGCCGCGTGCAGGC
GAGCGCGATGAACCATCCGCTTCTCGCGACGCTGCGCGGCGCGATCCCGCAGATCGCCGCGCACTACGAGGCGCGCGAGT
ACGGCCGCGCGCTGCGCCAGACGATGGAGCTCGCGGATGCGGTGAACGGCTACGTCGACTCCGCGAAGCCGTGGGAGCTC
GCGAAGGATCCGGCGAACGCGGTCGCGCTGCATGAAACCTGCAGCGTGAGCCTCGAGGCGTTCCGCCTGCTGTCGCTCGC
GCTCAAGCCGGTGCTGCCGCGCGTCGCGCAAGGCGTCGAGGCGTTCCTCGGCATCGCGCCGCTCACGTGGGCCGATGCGG
GCATGCCGCTGTCGCCCGAGCAGCCGGTCCGCGCATACCAGCATCTGATGACCCGCGTCGATCCGAAGCAGATCGACGCG
CTGCTCGCGGCGAACCGCGGCTCGCTGCAAGGCACGGCGGCGGCGGCCGAAGCCGGCGCGGCGAACGGCAACGGCGCCGG
CTCGAAGAACGGCAAAGGCGCCAAGGCGGCGGCGCAGCCCGCGGCGAGCGCGGCGAATGCCGACGACGGCGCGTCGCCGA
TCATCTCGATCGACGATTTCGCGAAGATCGACCTGCGCATCGCGAAGATCGTCGCGTGCCAGGCGGTCGAAGGCTCGGAC
AAGCTGCTGCAGCTCACGCTCGACGTCGGCGAGGAGAGGACCCGCAACGTGTTCTCCGGCATCAAGTCCGCATACCGGCC
CGAGCAGCTCGTCGGCAAGCTGACGGTGATGGTCGCGAACCTCGCGCCGCGCAAGATGAAGTTCGGCCTGTCCGAAGGGA
TGGTGCTCGCCGCGTCGGCGGCCGACGAGAAGGCCGAGCCCGGCCTCTACATCCTCGAGCCGCACAGCGGCGCGAAGCCC
GGCATGCGCGTGAAGTAA

Upstream 100 bases:

>100_bases
GCGCGATTCCGCGCGGTCCGCCCGCAGGTCGGCGCGCGGCCGCCCTGCTAGAATCGCGGCTTCCCTACGATTTTCCGCAA
GCAGTCCGTTCAGAGACCCT

Downstream 100 bases:

>100_bases
GCGCGCATCGCCACGGCGACGCATCCGCAAGGGCCGCGCAAGCGGCCCTTGTCGTTCTTGCGCGTCAGGCTCTCGCGCGT
GAGCGGCGCGGGGTCGTGTG

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS [H]

Number of amino acids: Translated: 725; Mature: 724

Protein sequence:

>725_residues
MSASDLTSVQAGAPQGRRQILVTSALPYANGQIHIGHLVEYIQTDIWVRTMRMHGHEIYYIGADDTHGTPVMLRAEQEGV
SPKQLIERVWREHKRDFDSFGVSFDNFYTTDSDENRVLSETIYLALKEAGFIAEREIEQAYDPVRQMFLPDRFIKGECPK
CHAKDQYGDSCEVCGTTYQPTDLIHPYSVVSGAAPVRKTSTHYFFRLSDPRCEAFLREWVSGLAQPEATNKMREWLGEAG
EAKLADWDISRDAPYFGFEIPGAPGKYFYVWLDAPVGYYASFKNLCQRRGLDFDAWIRKDSTTEQYHFIGKDILYFHTLF
WPAMLEFSGHRTPTNVFAHGFLTVDGAKMSKSRGTFITAQSYIDTGLNPEWLRYYFAAKLNATMEDIDLNLEDFQARVNS
DLVGKYVNIASRAAGFLLKRFDGRVQASAMNHPLLATLRGAIPQIAAHYEAREYGRALRQTMELADAVNGYVDSAKPWEL
AKDPANAVALHETCSVSLEAFRLLSLALKPVLPRVAQGVEAFLGIAPLTWADAGMPLSPEQPVRAYQHLMTRVDPKQIDA
LLAANRGSLQGTAAAAEAGAANGNGAGSKNGKGAKAAAQPAASAANADDGASPIISIDDFAKIDLRIAKIVACQAVEGSD
KLLQLTLDVGEERTRNVFSGIKSAYRPEQLVGKLTVMVANLAPRKMKFGLSEGMVLAASAADEKAEPGLYILEPHSGAKP
GMRVK

Sequences:

>Translated_725_residues
MSASDLTSVQAGAPQGRRQILVTSALPYANGQIHIGHLVEYIQTDIWVRTMRMHGHEIYYIGADDTHGTPVMLRAEQEGV
SPKQLIERVWREHKRDFDSFGVSFDNFYTTDSDENRVLSETIYLALKEAGFIAEREIEQAYDPVRQMFLPDRFIKGECPK
CHAKDQYGDSCEVCGTTYQPTDLIHPYSVVSGAAPVRKTSTHYFFRLSDPRCEAFLREWVSGLAQPEATNKMREWLGEAG
EAKLADWDISRDAPYFGFEIPGAPGKYFYVWLDAPVGYYASFKNLCQRRGLDFDAWIRKDSTTEQYHFIGKDILYFHTLF
WPAMLEFSGHRTPTNVFAHGFLTVDGAKMSKSRGTFITAQSYIDTGLNPEWLRYYFAAKLNATMEDIDLNLEDFQARVNS
DLVGKYVNIASRAAGFLLKRFDGRVQASAMNHPLLATLRGAIPQIAAHYEAREYGRALRQTMELADAVNGYVDSAKPWEL
AKDPANAVALHETCSVSLEAFRLLSLALKPVLPRVAQGVEAFLGIAPLTWADAGMPLSPEQPVRAYQHLMTRVDPKQIDA
LLAANRGSLQGTAAAAEAGAANGNGAGSKNGKGAKAAAQPAASAANADDGASPIISIDDFAKIDLRIAKIVACQAVEGSD
KLLQLTLDVGEERTRNVFSGIKSAYRPEQLVGKLTVMVANLAPRKMKFGLSEGMVLAASAADEKAEPGLYILEPHSGAKP
GMRVK
>Mature_724_residues
SASDLTSVQAGAPQGRRQILVTSALPYANGQIHIGHLVEYIQTDIWVRTMRMHGHEIYYIGADDTHGTPVMLRAEQEGVS
PKQLIERVWREHKRDFDSFGVSFDNFYTTDSDENRVLSETIYLALKEAGFIAEREIEQAYDPVRQMFLPDRFIKGECPKC
HAKDQYGDSCEVCGTTYQPTDLIHPYSVVSGAAPVRKTSTHYFFRLSDPRCEAFLREWVSGLAQPEATNKMREWLGEAGE
AKLADWDISRDAPYFGFEIPGAPGKYFYVWLDAPVGYYASFKNLCQRRGLDFDAWIRKDSTTEQYHFIGKDILYFHTLFW
PAMLEFSGHRTPTNVFAHGFLTVDGAKMSKSRGTFITAQSYIDTGLNPEWLRYYFAAKLNATMEDIDLNLEDFQARVNSD
LVGKYVNIASRAAGFLLKRFDGRVQASAMNHPLLATLRGAIPQIAAHYEAREYGRALRQTMELADAVNGYVDSAKPWELA
KDPANAVALHETCSVSLEAFRLLSLALKPVLPRVAQGVEAFLGIAPLTWADAGMPLSPEQPVRAYQHLMTRVDPKQIDAL
LAANRGSLQGTAAAAEAGAANGNGAGSKNGKGAKAAAQPAASAANADDGASPIISIDDFAKIDLRIAKIVACQAVEGSDK
LLQLTLDVGEERTRNVFSGIKSAYRPEQLVGKLTVMVANLAPRKMKFGLSEGMVLAASAADEKAEPGLYILEPHSGAKPG
MRVK

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation [H]

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI14043022, Length=526, Percent_Identity=27.3764258555133, Blast_Score=208, Evalue=2e-53,
Organism=Homo sapiens, GI24308436, Length=553, Percent_Identity=26.7631103074141, Blast_Score=160, Evalue=4e-39,
Organism=Escherichia coli, GI1788432, Length=715, Percent_Identity=52.7272727272727, Blast_Score=748, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17540794, Length=693, Percent_Identity=26.8398268398268, Blast_Score=236, Evalue=3e-62,
Organism=Caenorhabditis elegans, GI212645096, Length=548, Percent_Identity=22.992700729927, Blast_Score=123, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI71992781, Length=290, Percent_Identity=25.1724137931034, Blast_Score=94, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6321704, Length=564, Percent_Identity=26.9503546099291, Blast_Score=221, Evalue=2e-58,
Organism=Saccharomyces cerevisiae, GI6321610, Length=545, Percent_Identity=24.2201834862385, Blast_Score=117, Evalue=6e-27,
Organism=Drosophila melanogaster, GI24655311, Length=544, Percent_Identity=28.6764705882353, Blast_Score=222, Evalue=8e-58,
Organism=Drosophila melanogaster, GI21358483, Length=518, Percent_Identity=26.2548262548263, Blast_Score=136, Evalue=7e-32,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR015413
- InterPro:   IPR004495
- InterPro:   IPR014758
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR014729
- InterPro:   IPR002547
- InterPro:   IPR009080 [H]

Pfam domain/function: PF09334 tRNA-synt_1g; PF01588 tRNA_bind [H]

EC number: =6.1.1.10 [H]

Molecular weight: Translated: 79914; Mature: 79783

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS50886 TRBD ; PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSASDLTSVQAGAPQGRRQILVTSALPYANGQIHIGHLVEYIQTDIWVRTMRMHGHEIYY
CCCCCCCHHCCCCCCCCCEEEEEECCCCCCCCEEHHHHHHHHHHHHHHHHHHHCCCEEEE
IGADDTHGTPVMLRAEQEGVSPKQLIERVWREHKRDFDSFGVSFDNFYTTDSDENRVLSE
EECCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCHHHHCCCCCCCCCCCCCCCHHHHH
TIYLALKEAGFIAEREIEQAYDPVRQMFLPDRFIKGECPKCHAKDQYGDSCEVCGTTYQP
HHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCCCCCHHCCCCCCCC
TDLIHPYSVVSGAAPVRKTSTHYFFRLSDPRCEAFLREWVSGLAQPEATNKMREWLGEAG
CCCCCCHHHHCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCC
EAKLADWDISRDAPYFGFEIPGAPGKYFYVWLDAPVGYYASFKNLCQRRGLDFDAWIRKD
CCEEECCCCCCCCCCCCEECCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCHHHHCCCC
STTEQYHFIGKDILYFHTLFWPAMLEFSGHRTPTNVFAHGFLTVDGAKMSKSRGTFITAQ
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHEEEEEEEECCCCCCCCCCCEEEEH
SYIDTGLNPEWLRYYFAAKLNATMEDIDLNLEDFQARVNSDLVGKYVNIASRAAGFLLKR
HHHCCCCCHHHHHHHHHHHHCCCHHHHCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHH
FDGRVQASAMNHPLLATLRGAIPQIAAHYEAREYGRALRQTMELADAVNGYVDSAKPWEL
HCCCEEEHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
AKDPANAVALHETCSVSLEAFRLLSLALKPVLPRVAQGVEAFLGIAPLTWADAGMPLSPE
CCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
QPVRAYQHLMTRVDPKQIDALLAANRGSLQGTAAAAEAGAANGNGAGSKNGKGAKAAAQP
CHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCHHHCC
AASAANADDGASPIISIDDFAKIDLRIAKIVACQAVEGSDKLLQLTLDVGEERTRNVFSG
CCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHH
IKSAYRPEQLVGKLTVMVANLAPRKMKFGLSEGMVLAASAADEKAEPGLYILEPHSGAKP
HHHHCCHHHHHHHHHHHHHHCCCHHHHCCCCCCEEEEECCCCCCCCCCEEEECCCCCCCC
GMRVK
CCCCC
>Mature Secondary Structure 
SASDLTSVQAGAPQGRRQILVTSALPYANGQIHIGHLVEYIQTDIWVRTMRMHGHEIYY
CCCCCCHHCCCCCCCCCEEEEEECCCCCCCCEEHHHHHHHHHHHHHHHHHHHCCCEEEE
IGADDTHGTPVMLRAEQEGVSPKQLIERVWREHKRDFDSFGVSFDNFYTTDSDENRVLSE
EECCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCHHHHCCCCCCCCCCCCCCCHHHHH
TIYLALKEAGFIAEREIEQAYDPVRQMFLPDRFIKGECPKCHAKDQYGDSCEVCGTTYQP
HHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCCCCCHHCCCCCCCC
TDLIHPYSVVSGAAPVRKTSTHYFFRLSDPRCEAFLREWVSGLAQPEATNKMREWLGEAG
CCCCCCHHHHCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCC
EAKLADWDISRDAPYFGFEIPGAPGKYFYVWLDAPVGYYASFKNLCQRRGLDFDAWIRKD
CCEEECCCCCCCCCCCCEECCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCHHHHCCCC
STTEQYHFIGKDILYFHTLFWPAMLEFSGHRTPTNVFAHGFLTVDGAKMSKSRGTFITAQ
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHEEEEEEEECCCCCCCCCCCEEEEH
SYIDTGLNPEWLRYYFAAKLNATMEDIDLNLEDFQARVNSDLVGKYVNIASRAAGFLLKR
HHHCCCCCHHHHHHHHHHHHCCCHHHHCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHH
FDGRVQASAMNHPLLATLRGAIPQIAAHYEAREYGRALRQTMELADAVNGYVDSAKPWEL
HCCCEEEHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
AKDPANAVALHETCSVSLEAFRLLSLALKPVLPRVAQGVEAFLGIAPLTWADAGMPLSPE
CCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
QPVRAYQHLMTRVDPKQIDALLAANRGSLQGTAAAAEAGAANGNGAGSKNGKGAKAAAQP
CHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCHHHCC
AASAANADDGASPIISIDDFAKIDLRIAKIVACQAVEGSDKLLQLTLDVGEERTRNVFSG
CCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHH
IKSAYRPEQLVGKLTVMVANLAPRKMKFGLSEGMVLAASAADEKAEPGLYILEPHSGAKP
HHHHCCHHHHHHHHHHHHHHCCCHHHHCCCCCCEEEEECCCCCCCCCCEEEECCCCCCCC
GMRVK
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA