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The map label for this gene is fucA [C]

Identifier: 53718579

GI number: 53718579

Start: 1088737

End: 1089519

Strand: Reverse

Name: fucA [C]

Synonym: BPSL0937

Alternate gene names: 53718579

Gene position: 1089519-1088737 (Counterclockwise)

Preceding gene: 53718586

Following gene: 53718578

Centisome position: 26.74

GC content: 68.45

Gene sequence:

>783_bases
ATGGTCGACACATTGAAGATGGGCAAGGCCGAGCTCGTCGCGCTCGCGCAGCGGCGGCTCGACAACGAGATCAAGGACAG
CGGGTGGAGCGCGCGCGAGAAGCTCGCGCTCACCTGCCGGATCCTGTTCGACGACGGGCACGACTCCGGCCTCGCCGGGC
AGATCACGTGCCGCGCCGACGCGCCGCACACGTACTACACGCAGCGCCTCGGGCTCGGCTTCGACGAGATCGCCGAATCG
AACCTGCTGCAGGTCGATCAGGACCTCGAGGTCATCGGCGGCCAGGGCATTCCGAATCCCGCGAACCGCTTTCACACGTG
GATTTATCGCGCGCGCCCCGACGTCAACTGCATCATCCACACGCACCCGACGCACGTCGCCGCGCTGTCGATGCTCGAGA
CGCCGCTCGTCGTGTCGCACATGGACACCTGTCCGCTTCACGGCGACTGCGCGTTCCTGAAGGATTGGCCGGGCGTGCCC
GTCGGCAACGAGGAAGGCGAGATCATCTCGAACGCGCTCGGCGACAAGCGCGCGATCCTGCTGTCGCATCACGGCCAGCT
CGTCGTCGGCAAGACGATCGAAGAGGCGTGCATGCTCGCGATCCTGATCGAGCGCGCGGCGCGGCTGCAACTGCTCGCGA
TGGCGGCCGGCGCGATCGCGCCGATTCCGCCCGCGCTCGCGGCCGACGCGCACGACTGGATCTCGACGCCCAAGCGCGAC
GCGGTGACCTTCGCGTACTACGCGCGCCGCGCGCTACGCCGCCACCCCGACTGCCTCGACTGA

Upstream 100 bases:

>100_bases
ATCTCATGACACTTTGTGTCCTATAATCGACTTCGTGTTCTTGATGTGTCGCAGCGACGCACCAAGAACACCGTACATTC
GTTCACGTCATGGAGGCGGT

Downstream 100 bases:

>100_bases
TCCGCCGAATCGCCCACGCGTCGCACCGGCGGCGAGCCGACGACACCCGAAAACATCGAACATTCCGGAGCACCCCGACT
CATGTCCAACCTCTTGCAAG

Product: class II aldolase/adducin domain-containing protein

Products: Dihydroxyacetone phosphate; L-Lactaldehyde [C]

Alternate protein names: NA

Number of amino acids: Translated: 260; Mature: 260

Protein sequence:

>260_residues
MVDTLKMGKAELVALAQRRLDNEIKDSGWSAREKLALTCRILFDDGHDSGLAGQITCRADAPHTYYTQRLGLGFDEIAES
NLLQVDQDLEVIGGQGIPNPANRFHTWIYRARPDVNCIIHTHPTHVAALSMLETPLVVSHMDTCPLHGDCAFLKDWPGVP
VGNEEGEIISNALGDKRAILLSHHGQLVVGKTIEEACMLAILIERAARLQLLAMAAGAIAPIPPALAADAHDWISTPKRD
AVTFAYYARRALRRHPDCLD

Sequences:

>Translated_260_residues
MVDTLKMGKAELVALAQRRLDNEIKDSGWSAREKLALTCRILFDDGHDSGLAGQITCRADAPHTYYTQRLGLGFDEIAES
NLLQVDQDLEVIGGQGIPNPANRFHTWIYRARPDVNCIIHTHPTHVAALSMLETPLVVSHMDTCPLHGDCAFLKDWPGVP
VGNEEGEIISNALGDKRAILLSHHGQLVVGKTIEEACMLAILIERAARLQLLAMAAGAIAPIPPALAADAHDWISTPKRD
AVTFAYYARRALRRHPDCLD
>Mature_260_residues
MVDTLKMGKAELVALAQRRLDNEIKDSGWSAREKLALTCRILFDDGHDSGLAGQITCRADAPHTYYTQRLGLGFDEIAES
NLLQVDQDLEVIGGQGIPNPANRFHTWIYRARPDVNCIIHTHPTHVAALSMLETPLVVSHMDTCPLHGDCAFLKDWPGVP
VGNEEGEIISNALGDKRAILLSHHGQLVVGKTIEEACMLAILIERAARLQLLAMAAGAIAPIPPALAADAHDWISTPKRD
AVTFAYYARRALRRHPDCLD

Specific function: Fucose metabolism; third step. [C]

COG id: COG0235

COG function: function code G; Ribulose-5-phosphate 4-epimerase and related epimerases and aldolases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aldolase class II family [H]

Homologues:

Organism=Homo sapiens, GI29826325, Length=193, Percent_Identity=27.979274611399, Blast_Score=68, Evalue=9e-12,
Organism=Homo sapiens, GI29826323, Length=193, Percent_Identity=27.979274611399, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI29826321, Length=193, Percent_Identity=27.979274611399, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI29826319, Length=191, Percent_Identity=28.2722513089005, Blast_Score=66, Evalue=3e-11,
Organism=Homo sapiens, GI9943848, Length=156, Percent_Identity=32.6923076923077, Blast_Score=65, Evalue=5e-11,
Organism=Homo sapiens, GI9951927, Length=156, Percent_Identity=32.6923076923077, Blast_Score=65, Evalue=6e-11,
Organism=Homo sapiens, GI62912453, Length=156, Percent_Identity=32.6923076923077, Blast_Score=65, Evalue=6e-11,
Organism=Escherichia coli, GI1789164, Length=199, Percent_Identity=28.643216080402, Blast_Score=71, Evalue=8e-14,
Organism=Caenorhabditis elegans, GI71989560, Length=221, Percent_Identity=29.8642533936652, Blast_Score=74, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI71989550, Length=221, Percent_Identity=29.8642533936652, Blast_Score=74, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI71989555, Length=221, Percent_Identity=29.8642533936652, Blast_Score=74, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI71989544, Length=221, Percent_Identity=29.8642533936652, Blast_Score=74, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI71989541, Length=221, Percent_Identity=29.8642533936652, Blast_Score=74, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI115533218, Length=193, Percent_Identity=26.9430051813472, Blast_Score=70, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI115533216, Length=193, Percent_Identity=26.9430051813472, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI281363755, Length=192, Percent_Identity=32.8125, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI281363751, Length=192, Percent_Identity=32.8125, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24655690, Length=192, Percent_Identity=32.8125, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24655685, Length=192, Percent_Identity=32.8125, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI281363763, Length=192, Percent_Identity=32.8125, Blast_Score=76, Evalue=3e-14,
Organism=Drosophila melanogaster, GI281363757, Length=192, Percent_Identity=32.8125, Blast_Score=76, Evalue=3e-14,
Organism=Drosophila melanogaster, GI17136738, Length=192, Percent_Identity=32.8125, Blast_Score=76, Evalue=3e-14,
Organism=Drosophila melanogaster, GI281363761, Length=192, Percent_Identity=32.8125, Blast_Score=76, Evalue=3e-14,
Organism=Drosophila melanogaster, GI281363759, Length=192, Percent_Identity=32.8125, Blast_Score=76, Evalue=3e-14,
Organism=Drosophila melanogaster, GI281363753, Length=192, Percent_Identity=32.8125, Blast_Score=76, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001303 [H]

Pfam domain/function: PF00596 Aldolase_II [H]

EC number: 4.1.2.17 [C]

Molecular weight: Translated: 28505; Mature: 28505

Theoretical pI: Translated: 6.22; Mature: 6.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVDTLKMGKAELVALAQRRLDNEIKDSGWSAREKLALTCRILFDDGHDSGLAGQITCRAD
CCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEEEECCCCCCCEEEEEEECC
APHTYYTQRLGLGFDEIAESNLLQVDQDLEVIGGQGIPNPANRFHTWIYRARPDVNCIIH
CCCHHHHHHHCCCHHHHHCCCCEECCCCHHEECCCCCCCHHHHHEEEEEECCCCCEEEEE
THPTHVAALSMLETPLVVSHMDTCPLHGDCAFLKDWPGVPVGNEEGEIISNALGDKRAIL
ECCHHHHHHHHHHCCHHHHCCCCCCCCCCEEEECCCCCCCCCCCCCHHHHHHCCCCEEEE
LSHHGQLVVGKTIEEACMLAILIERAARLQLLAMAAGAIAPIPPALAADAHDWISTPKRD
EECCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCHHHHCCCCCH
AVTFAYYARRALRRHPDCLD
HHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MVDTLKMGKAELVALAQRRLDNEIKDSGWSAREKLALTCRILFDDGHDSGLAGQITCRAD
CCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEEEECCCCCCCEEEEEEECC
APHTYYTQRLGLGFDEIAESNLLQVDQDLEVIGGQGIPNPANRFHTWIYRARPDVNCIIH
CCCHHHHHHHCCCHHHHHCCCCEECCCCHHEECCCCCCCHHHHHEEEEEECCCCCEEEEE
THPTHVAALSMLETPLVVSHMDTCPLHGDCAFLKDWPGVPVGNEEGEIISNALGDKRAIL
ECCHHHHHHHHHHCCHHHHCCCCCCCCCCEEEECCCCCCCCCCCCCHHHHHHCCCCEEEE
LSHHGQLVVGKTIEEACMLAILIERAARLQLLAMAAGAIAPIPPALAADAHDWISTPKRD
EECCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCHHHHCCCCCH
AVTFAYYARRALRRHPDCLD
HHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Ca2+; Co2+; Mg2+; Mn2+; Zn2+ [C]

Kcat value (1/min): 19.3 [C]

Specific activity: 21.0

Km value (mM): 0.7 {L-fuculose} [C]

Substrates: L-Fuculose 1-phosphate [C]

Specific reaction: L-Fuculose 1-phosphate <==> Dihydroxyacetone phosphate + L-Lactaldehyde [C]

General reaction: Elimination of an aldehyde C-C bond; Cleavage [C]

Inhibitor: EDTA; Phosphoglycolohydroxamate [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11259647 [H]