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The map label for this gene is proP [H]

Identifier: 53718412

GI number: 53718412

Start: 898930

End: 900600

Strand: Reverse

Name: proP [H]

Synonym: BPSL0773

Alternate gene names: 53718412

Gene position: 900600-898930 (Counterclockwise)

Preceding gene: 53718413

Following gene: 53718411

Centisome position: 22.1

GC content: 64.45

Gene sequence:

>1671_bases
TTGGAGACATTCATGGCAACGGTTGGCGGACAAATTGCGCACGCGCCGATGACGGGCGACGAAAAGCGCGTGATCTTCGC
ATCGTCGCTCGGCACCGTGTTCGAGTGGTACGACTTCTATCTGGCCGGCTCGCTCGCGGCCTACATCAGCAAGAGCTTCT
TTTCAGGCGTCAACCCGACCGCCGCGTTCATCTTCACGCTGCTCGGCTTCGCGGCGGGCTTCGCCGTCCGACCGTTCGGC
GCGATCGTGTTCGGCCGCCTCGGCGACCTCGTGGGACGCAAGCATACGTTCCTCATCACGATCGTGATCATGGGGATCTC
GACGTTCGTCGTCGGCTTCCTGCCGGGCTACGCGTCGATCGGCATCGCCGCGCCCGTGATCTTCATCGCGATGCGGCTGC
TGCAAGGCCTCGCGCTCGGCGGCGAATACGGCGGCGCGGCGACATACGTGGCCGAGCATGCACCCTCGCACCGCCGCGGC
TTCTATACGTCGTGGATCCAGACGACCGCGACGCTCGGCCTGTTCCTGTCGCTCCTCGTGATTCTCGGCGTGCGCACCGC
GATCGGCGAGGAAGCATTCGGCAGTTGGGGCTGGCGCGTGCCGTTCGTCGCGTCGATCCTGCTGCTCGCCGTATCCGTGT
GGATCCGCCTGCAACTGAACGAATCGCCGGTGTTCCTGCGGATCAAGGCCGAGGGCAAGACGTCGAAGGCGCCGCTCACC
GAAGCGTTCGGCCAGTGGAAGAACCTGAAGATCGTGATCCTCGCGCTCATCGGGCTGACGGCGGGCCAGGCGGTGGTCTG
GTACACGGGCCAGTTCTATGCGCTCTTCTTCCTCACGCAAACGCTGAAGGTCGACGGCGCGAGCGCGAACATCCTGATCG
CGCTCGCCCTTCTGATCGGCACGCCGTTCTTCGTGTTCTTCGGCTCGCTGTCCGACAGGATCGGCCGCAAGCCGATCATC
CTCGCCGGCTGCCTGATCGCCGCGCTCACCTACTTCCCGCTCTTCAAGGCGCTCACGCACTTCGCGAATCCGGCGCTCGA
GGCGGCGACGCAAAAGGCGCCGATCACGGTGGTCGCCAATCCCGACGAATGCTCGTTCCAGTTCAACCCGGTGGGCACGG
CGAAGTTCACGTCGTCCTGCGACATCGCGAAAAGCGCGCTCGCGAAATCGGGCCTCAATTACGAGAACGTCGCCGCGCCG
GCGGGCACGCTTGCGCAGATCAAGATCGGCGACACGGCGATCGCCACGTACGACGGCAAGGCCGCCGACGCAAAAGACCA
GGCGAAGGCGTTCGACAAGACGCTCGCGTCGACGCTGAAGGCGGCGGGCTACCCGGCGAAAGCCGACCCGGCCCGGCTCA
ACTGGCCGATGACGACGGTGATCCTCGCGATACTCGTGATCTACGTGACGATGGTGTACGGGCCGATCGCGGCGATGCTG
GTCGAGATGTTCCCGACGCGGATTCGCTATACGTCGATGTCGCTGCCGTATCACATCGGCAACGGCTGGTTCGGCGGCTT
CCTGCCGGCGACGGCGTTCGCGATCGTCGCGGCGAAGGGCGACATCTATTCGGGGCTCTGGTATCCGATCGGCGTCGCGC
TCGTCACGTTCGTGATCGGACTGCTGTTCGTGAAGGAAACCAAGGATTCGAATATCTACGCGCAGGATTGA

Upstream 100 bases:

>100_bases
GCGCTTTTTTGACGCGCCGAGTCAGTGCGCCGTAAGTTTGCCCTCCAATAATCGACTCGGAGCCGCCCTCCCGGGAGGGC
GCGCGACCCCTATATCAAAA

Downstream 100 bases:

>100_bases
CGAAGGCGGGCTGCGGCGCGCCGGCGTATGGGAGAGGGCGGGCGGCGGGGGGCCGGCGGTGGAGGTTTGGACCACCGCCG
GACGAAGGTCGGCGGCACGC

Product: putative transmembrane transport protein

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Proline porter II; PPII [H]

Number of amino acids: Translated: 556; Mature: 556

Protein sequence:

>556_residues
METFMATVGGQIAHAPMTGDEKRVIFASSLGTVFEWYDFYLAGSLAAYISKSFFSGVNPTAAFIFTLLGFAAGFAVRPFG
AIVFGRLGDLVGRKHTFLITIVIMGISTFVVGFLPGYASIGIAAPVIFIAMRLLQGLALGGEYGGAATYVAEHAPSHRRG
FYTSWIQTTATLGLFLSLLVILGVRTAIGEEAFGSWGWRVPFVASILLLAVSVWIRLQLNESPVFLRIKAEGKTSKAPLT
EAFGQWKNLKIVILALIGLTAGQAVVWYTGQFYALFFLTQTLKVDGASANILIALALLIGTPFFVFFGSLSDRIGRKPII
LAGCLIAALTYFPLFKALTHFANPALEAATQKAPITVVANPDECSFQFNPVGTAKFTSSCDIAKSALAKSGLNYENVAAP
AGTLAQIKIGDTAIATYDGKAADAKDQAKAFDKTLASTLKAAGYPAKADPARLNWPMTTVILAILVIYVTMVYGPIAAML
VEMFPTRIRYTSMSLPYHIGNGWFGGFLPATAFAIVAAKGDIYSGLWYPIGVALVTFVIGLLFVKETKDSNIYAQD

Sequences:

>Translated_556_residues
METFMATVGGQIAHAPMTGDEKRVIFASSLGTVFEWYDFYLAGSLAAYISKSFFSGVNPTAAFIFTLLGFAAGFAVRPFG
AIVFGRLGDLVGRKHTFLITIVIMGISTFVVGFLPGYASIGIAAPVIFIAMRLLQGLALGGEYGGAATYVAEHAPSHRRG
FYTSWIQTTATLGLFLSLLVILGVRTAIGEEAFGSWGWRVPFVASILLLAVSVWIRLQLNESPVFLRIKAEGKTSKAPLT
EAFGQWKNLKIVILALIGLTAGQAVVWYTGQFYALFFLTQTLKVDGASANILIALALLIGTPFFVFFGSLSDRIGRKPII
LAGCLIAALTYFPLFKALTHFANPALEAATQKAPITVVANPDECSFQFNPVGTAKFTSSCDIAKSALAKSGLNYENVAAP
AGTLAQIKIGDTAIATYDGKAADAKDQAKAFDKTLASTLKAAGYPAKADPARLNWPMTTVILAILVIYVTMVYGPIAAML
VEMFPTRIRYTSMSLPYHIGNGWFGGFLPATAFAIVAAKGDIYSGLWYPIGVALVTFVIGLLFVKETKDSNIYAQD
>Mature_556_residues
METFMATVGGQIAHAPMTGDEKRVIFASSLGTVFEWYDFYLAGSLAAYISKSFFSGVNPTAAFIFTLLGFAAGFAVRPFG
AIVFGRLGDLVGRKHTFLITIVIMGISTFVVGFLPGYASIGIAAPVIFIAMRLLQGLALGGEYGGAATYVAEHAPSHRRG
FYTSWIQTTATLGLFLSLLVILGVRTAIGEEAFGSWGWRVPFVASILLLAVSVWIRLQLNESPVFLRIKAEGKTSKAPLT
EAFGQWKNLKIVILALIGLTAGQAVVWYTGQFYALFFLTQTLKVDGASANILIALALLIGTPFFVFFGSLSDRIGRKPII
LAGCLIAALTYFPLFKALTHFANPALEAATQKAPITVVANPDECSFQFNPVGTAKFTSSCDIAKSALAKSGLNYENVAAP
AGTLAQIKIGDTAIATYDGKAADAKDQAKAFDKTLASTLKAAGYPAKADPARLNWPMTTVILAILVIYVTMVYGPIAAML
VEMFPTRIRYTSMSLPYHIGNGWFGGFLPATAFAIVAAKGDIYSGLWYPIGVALVTFVIGLLFVKETKDSNIYAQD

Specific function: Proton symporter that senses osmotic shifts and responds by importing osmolytes such as proline, glycine betaine, stachydrine, pipecolic acid, ectoine and taurine. It is both an osmosensor and an osmoregulator which is available to participate early in th

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=328, Percent_Identity=35.9756097560976, Blast_Score=197, Evalue=1e-51,
Organism=Escherichia coli, GI1788292, Length=328, Percent_Identity=35.0609756097561, Blast_Score=186, Evalue=4e-48,
Organism=Escherichia coli, GI1788942, Length=333, Percent_Identity=34.8348348348348, Blast_Score=171, Evalue=1e-43,
Organism=Escherichia coli, GI1789941, Length=352, Percent_Identity=35.7954545454545, Blast_Score=151, Evalue=1e-37,
Organism=Escherichia coli, GI87082231, Length=223, Percent_Identity=29.1479820627803, Blast_Score=79, Evalue=7e-16,
Organism=Escherichia coli, GI87082404, Length=317, Percent_Identity=24.2902208201893, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR015041
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF08946 Osmo_CC; PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 59502; Mature: 59502

Theoretical pI: Translated: 9.60; Mature: 9.60

Prosite motif: PS50850 MFS ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METFMATVGGQIAHAPMTGDEKRVIFASSLGTVFEWYDFYLAGSLAAYISKSFFSGVNPT
CCCHHHHHCCCEEECCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
AAFIFTLLGFAAGFAVRPFGAIVFGRLGDLVGRKHTFLITIVIMGISTFVVGFLPGYASI
HHHHHHHHHHHHCCHHCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCHHHH
GIAAPVIFIAMRLLQGLALGGEYGGAATYVAEHAPSHRRGFYTSWIQTTATLGLFLSLLV
HHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHH
ILGVRTAIGEEAFGSWGWRVPFVASILLLAVSVWIRLQLNESPVFLRIKAEGKTSKAPLT
HHHHHHHHCHHHHCCCCCCHHHHHHHHHHHHHHHEEEEECCCCEEEEEEECCCCCCCCHH
EAFGQWKNLKIVILALIGLTAGQAVVWYTGQFYALFFLTQTLKVDGASANILIALALLIG
HHHCCCCCHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHEECCCCCHHHHHHHHHHC
TPFFVFFGSLSDRIGRKPIILAGCLIAALTYFPLFKALTHFANPALEAATQKAPITVVAN
CHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCEEEEEC
PDECSFQFNPVGTAKFTSSCDIAKSALAKSGLNYENVAAPAGTLAQIKIGDTAIATYDGK
CCCCCEEECCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCEEEEECCCC
AADAKDQAKAFDKTLASTLKAAGYPAKADPARLNWPMTTVILAILVIYVTMVYGPIAAML
CCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
VEMFPTRIRYTSMSLPYHIGNGWFGGFLPATAFAIVAAKGDIYSGLWYPIGVALVTFVIG
HHHHHHHEEEEEECCCEEECCCCCCHHHHHHHHHHHEECCCHHCCHHHHHHHHHHHHHHH
LLFVKETKDSNIYAQD
HHHHHCCCCCCCEECC
>Mature Secondary Structure
METFMATVGGQIAHAPMTGDEKRVIFASSLGTVFEWYDFYLAGSLAAYISKSFFSGVNPT
CCCHHHHHCCCEEECCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
AAFIFTLLGFAAGFAVRPFGAIVFGRLGDLVGRKHTFLITIVIMGISTFVVGFLPGYASI
HHHHHHHHHHHHCCHHCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCHHHH
GIAAPVIFIAMRLLQGLALGGEYGGAATYVAEHAPSHRRGFYTSWIQTTATLGLFLSLLV
HHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHH
ILGVRTAIGEEAFGSWGWRVPFVASILLLAVSVWIRLQLNESPVFLRIKAEGKTSKAPLT
HHHHHHHHCHHHHCCCCCCHHHHHHHHHHHHHHHEEEEECCCCEEEEEEECCCCCCCCHH
EAFGQWKNLKIVILALIGLTAGQAVVWYTGQFYALFFLTQTLKVDGASANILIALALLIG
HHHCCCCCHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHEECCCCCHHHHHHHHHHC
TPFFVFFGSLSDRIGRKPIILAGCLIAALTYFPLFKALTHFANPALEAATQKAPITVVAN
CHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCEEEEEC
PDECSFQFNPVGTAKFTSSCDIAKSALAKSGLNYENVAAPAGTLAQIKIGDTAIATYDGK
CCCCCEEECCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCEEEEECCCC
AADAKDQAKAFDKTLASTLKAAGYPAKADPARLNWPMTTVILAILVIYVTMVYGPIAAML
CCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
VEMFPTRIRYTSMSLPYHIGNGWFGGFLPATAFAIVAAKGDIYSGLWYPIGVALVTFVIG
HHHHHHHEEEEEECCCEEECCCCCCHHHHHHHHHHHEECCCHHCCHHHHHHHHHHHHHHH
LLFVKETKDSNIYAQD
HHHHHCCCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]