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The map label for this gene is glpK

Identifier: 53718330

GI number: 53718330

Start: 782878

End: 784380

Strand: Reverse

Name: glpK

Synonym: BPSL0687

Alternate gene names: 53718330

Gene position: 784380-782878 (Counterclockwise)

Preceding gene: 53718331

Following gene: 53718329

Centisome position: 19.25

GC content: 67.33

Gene sequence:

>1503_bases
ATGCAGGATCAGTACATCCTCGCGCTCGACCAGGGCACGACCAGCTCCCGCGCCATGCTGTTCGATCGACAAGGCAACAT
CGTATCGATCGCGCAGAAGGAATTCGAACAAATTTATCCGCAGCCGGGCTGGGTCGAGCACGATCCCCAGGAGATCTGGT
CGACGCAGGCGGGCGTCGCGGCCGAGGCCGTCACGCGCGTCGGCCTGAACGGCACGGCGATCGCCGCGATCGGCATCACG
AACCAGCGCGAGACGACGATCGTCTGGGATCGCGAGACGGGTCATCCGATCTACAACGCGATCGTCTGGCAGGATCGCCG
CACCGCCGATTTCTGCGACAAGCTCAAGGCGCAGGGCCTCAGCGAGAAGGTGCGCGCGAAGACGGGCCTGCCGATCGACT
CGTACTTCTCGGCCACCAAGATCCGCTGGATTCTCGACAACGTCGAGGGCGCGCGCGAGAAGGCGCGCCAGGGCAAGCTC
GCGTTCGGCACGGTCGACAGCTGGCTCGTGTGGAACTTCACGAAGCACGAGCTGCACGTGACCGACGTGACGAACGCGTC
GCGCACGATGCTCTTCAACATCCACACGCTCGACTGGGACGACGAGTTGCTCGACGCACTCGAGATTCCGCGCAGCATGC
TGCCGCAAGTGCGCGCGTCCTCCGAGATCTACGGGCCGACGAAGACCACCGTGTTCGCCTCGAAGATTCCGCTCGCCGGC
ATCGCGGGCGATCAGCAGGCCGCGCTCTTCGGCCAGATGTGCACGAGCTCGGGCATGGTGAAGAACACCTACGGCACCGG
CTGCTTCCTGATGATGAACACCGGCGAGACGCCGATCGAATCGCGGAACAACCTCGTCACGACGATCGCCTGGCAAGTCG
ACGGCAAGGTCAGCTACGCGCTCGAAGGCAGCATCTTCATCGCGGGCGCGGTCGTGCAGTGGCTGCGCGACGGGCTCGGC
ATCATCAAGAGCGCGTCGGAGATCGAGGCGCTCGCCGCCGGCGTGCCGCACACGGACGGCGTCTATCTCGTGCCCGCGTT
CGCCGGCCTCGGCGCGCCGCACTGGAACGCGCGCGCCCGCGGCTCGCTGTTCGGCGTCACGCGCGGCACGACGTCCGCGC
ACCTCGCGCGCGCGGCGCTCGATTCGATCGCGTATCAATCGCTCGACGTGCTCAAGGCCATGGAGGCCGATTCGGGCATC
CGCATCGGCGAGCTGCGCGTGGACGGCGGCGCGAGCGCGAACAACCTGCTGATGCAGTTCCAGGCGGATCTGCTCGGCGT
CGACGCGGTGCGCCCGCGCATCACCGAGACGACCGCGCTCGGCGCCGCGTATCTCGCGGGCCTCGCGATCGGCTACTGGC
AGAACGTCGACGAGCTGCACAGCCAATGGCAGCTCGAGCGCCGCTTCGCGCCTTCGATGCAGGCCGAGCAGGTGGGCTCT
TGCCTCGCCGGCTGGCAGCGCGCGGTGCGCGCGGCGAAGGCATGGGCGGACGACACGCAGTAA

Upstream 100 bases:

>100_bases
GCGCACGACGCCGGCACCGACGAACGACGACAGGGAAACATCAGGAGTGCGCCGCGCCCGCGGCGCCAATCACAACTACG
TACGGATGGAGATGAGACAG

Downstream 100 bases:

>100_bases
GCCCGTTCGTTCGGCATCCGTATCGATCACATTTCAAACACATATTCACAACAATCAGGCGGGCCGCGCGACGCAAGCCG
CGAGCCCGCCATACCGGTAG

Product: glycerol kinase

Products: NA

Alternate protein names: ATP:glycerol 3-phosphotransferase; Glycerokinase; GK

Number of amino acids: Translated: 500; Mature: 500

Protein sequence:

>500_residues
MQDQYILALDQGTTSSRAMLFDRQGNIVSIAQKEFEQIYPQPGWVEHDPQEIWSTQAGVAAEAVTRVGLNGTAIAAIGIT
NQRETTIVWDRETGHPIYNAIVWQDRRTADFCDKLKAQGLSEKVRAKTGLPIDSYFSATKIRWILDNVEGAREKARQGKL
AFGTVDSWLVWNFTKHELHVTDVTNASRTMLFNIHTLDWDDELLDALEIPRSMLPQVRASSEIYGPTKTTVFASKIPLAG
IAGDQQAALFGQMCTSSGMVKNTYGTGCFLMMNTGETPIESRNNLVTTIAWQVDGKVSYALEGSIFIAGAVVQWLRDGLG
IIKSASEIEALAAGVPHTDGVYLVPAFAGLGAPHWNARARGSLFGVTRGTTSAHLARAALDSIAYQSLDVLKAMEADSGI
RIGELRVDGGASANNLLMQFQADLLGVDAVRPRITETTALGAAYLAGLAIGYWQNVDELHSQWQLERRFAPSMQAEQVGS
CLAGWQRAVRAAKAWADDTQ

Sequences:

>Translated_500_residues
MQDQYILALDQGTTSSRAMLFDRQGNIVSIAQKEFEQIYPQPGWVEHDPQEIWSTQAGVAAEAVTRVGLNGTAIAAIGIT
NQRETTIVWDRETGHPIYNAIVWQDRRTADFCDKLKAQGLSEKVRAKTGLPIDSYFSATKIRWILDNVEGAREKARQGKL
AFGTVDSWLVWNFTKHELHVTDVTNASRTMLFNIHTLDWDDELLDALEIPRSMLPQVRASSEIYGPTKTTVFASKIPLAG
IAGDQQAALFGQMCTSSGMVKNTYGTGCFLMMNTGETPIESRNNLVTTIAWQVDGKVSYALEGSIFIAGAVVQWLRDGLG
IIKSASEIEALAAGVPHTDGVYLVPAFAGLGAPHWNARARGSLFGVTRGTTSAHLARAALDSIAYQSLDVLKAMEADSGI
RIGELRVDGGASANNLLMQFQADLLGVDAVRPRITETTALGAAYLAGLAIGYWQNVDELHSQWQLERRFAPSMQAEQVGS
CLAGWQRAVRAAKAWADDTQ
>Mature_500_residues
MQDQYILALDQGTTSSRAMLFDRQGNIVSIAQKEFEQIYPQPGWVEHDPQEIWSTQAGVAAEAVTRVGLNGTAIAAIGIT
NQRETTIVWDRETGHPIYNAIVWQDRRTADFCDKLKAQGLSEKVRAKTGLPIDSYFSATKIRWILDNVEGAREKARQGKL
AFGTVDSWLVWNFTKHELHVTDVTNASRTMLFNIHTLDWDDELLDALEIPRSMLPQVRASSEIYGPTKTTVFASKIPLAG
IAGDQQAALFGQMCTSSGMVKNTYGTGCFLMMNTGETPIESRNNLVTTIAWQVDGKVSYALEGSIFIAGAVVQWLRDGLG
IIKSASEIEALAAGVPHTDGVYLVPAFAGLGAPHWNARARGSLFGVTRGTTSAHLARAALDSIAYQSLDVLKAMEADSGI
RIGELRVDGGASANNLLMQFQADLLGVDAVRPRITETTALGAAYLAGLAIGYWQNVDELHSQWQLERRFAPSMQAEQVGS
CLAGWQRAVRAAKAWADDTQ

Specific function: Key enzyme in the regulation of glycerol uptake and metabolism

COG id: COG0554

COG function: function code C; Glycerol kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FGGY kinase family

Homologues:

Organism=Homo sapiens, GI189409120, Length=502, Percent_Identity=45.6175298804781, Blast_Score=442, Evalue=1e-124,
Organism=Homo sapiens, GI4504007, Length=502, Percent_Identity=45.6175298804781, Blast_Score=442, Evalue=1e-124,
Organism=Homo sapiens, GI41393575, Length=502, Percent_Identity=45.2191235059761, Blast_Score=440, Evalue=1e-123,
Organism=Homo sapiens, GI42794763, Length=507, Percent_Identity=45.1676528599605, Blast_Score=439, Evalue=1e-123,
Organism=Homo sapiens, GI88196792, Length=511, Percent_Identity=31.7025440313112, Blast_Score=310, Evalue=2e-84,
Organism=Escherichia coli, GI1790361, Length=497, Percent_Identity=60.5633802816901, Blast_Score=659, Evalue=0.0,
Organism=Escherichia coli, GI1790005, Length=504, Percent_Identity=25.5952380952381, Blast_Score=118, Evalue=8e-28,
Organism=Escherichia coli, GI1789168, Length=493, Percent_Identity=24.5436105476673, Blast_Score=113, Evalue=3e-26,
Organism=Escherichia coli, GI1789987, Length=483, Percent_Identity=24.0165631469979, Blast_Score=106, Evalue=4e-24,
Organism=Escherichia coli, GI1787789, Length=478, Percent_Identity=25.1046025104602, Blast_Score=91, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17535599, Length=500, Percent_Identity=42, Blast_Score=401, Evalue=1e-112,
Organism=Caenorhabditis elegans, GI71986140, Length=515, Percent_Identity=24.2718446601942, Blast_Score=175, Evalue=4e-44,
Organism=Caenorhabditis elegans, GI71986147, Length=255, Percent_Identity=30.1960784313725, Blast_Score=125, Evalue=5e-29,
Organism=Saccharomyces cerevisiae, GI6321755, Length=491, Percent_Identity=37.6782077393075, Blast_Score=305, Evalue=8e-84,
Organism=Drosophila melanogaster, GI17864214, Length=505, Percent_Identity=41.980198019802, Blast_Score=395, Evalue=1e-110,
Organism=Drosophila melanogaster, GI24655555, Length=483, Percent_Identity=42.0289855072464, Blast_Score=385, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24655560, Length=483, Percent_Identity=42.0289855072464, Blast_Score=385, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24655570, Length=483, Percent_Identity=42.0289855072464, Blast_Score=385, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24655574, Length=483, Percent_Identity=42.0289855072464, Blast_Score=385, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24655565, Length=483, Percent_Identity=42.0289855072464, Blast_Score=385, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24654573, Length=445, Percent_Identity=42.4719101123596, Blast_Score=363, Evalue=1e-100,
Organism=Drosophila melanogaster, GI19922058, Length=532, Percent_Identity=32.7067669172932, Blast_Score=275, Evalue=4e-74,
Organism=Drosophila melanogaster, GI24652871, Length=499, Percent_Identity=33.4669338677355, Blast_Score=261, Evalue=8e-70,
Organism=Drosophila melanogaster, GI24656367, Length=513, Percent_Identity=30.2144249512671, Blast_Score=237, Evalue=1e-62,
Organism=Drosophila melanogaster, GI21357867, Length=525, Percent_Identity=29.3333333333333, Blast_Score=234, Evalue=9e-62,
Organism=Drosophila melanogaster, GI24656372, Length=367, Percent_Identity=29.700272479564, Blast_Score=171, Evalue=1e-42,
Organism=Drosophila melanogaster, GI45552909, Length=308, Percent_Identity=28.2467532467532, Blast_Score=127, Evalue=2e-29,

Paralogues:

None

Copy number: 2,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): GLPK_BURPS (Q63X50)

Other databases:

- EMBL:   BX571965
- RefSeq:   YP_107316.1
- HSSP:   P0A6F3
- ProteinModelPortal:   Q63X50
- SMR:   Q63X50
- GeneID:   3092942
- GenomeReviews:   BX571965_GR
- KEGG:   bps:BPSL0687
- HOGENOM:   HBG511469
- OMA:   KPSSEVY
- ProtClustDB:   PRK00047
- BioCyc:   BPSE272560:BPSL0687-MONOMER
- BRENDA:   2.7.1.30
- HAMAP:   MF_00186
- InterPro:   IPR000577
- InterPro:   IPR018485
- InterPro:   IPR018483
- InterPro:   IPR018484
- InterPro:   IPR005999
- PANTHER:   PTHR10196
- PANTHER:   PTHR10196:SF9
- TIGRFAMs:   TIGR01311

Pfam domain/function: PF02782 FGGY_C; PF00370 FGGY_N

EC number: =2.7.1.30

Molecular weight: Translated: 54534; Mature: 54534

Theoretical pI: Translated: 5.47; Mature: 5.47

Prosite motif: PS00933 FGGY_KINASES_1; PS00445 FGGY_KINASES_2

Important sites: BINDING 13-13 BINDING 17-17 BINDING 83-83 BINDING 135-135 BINDING 244-244 BINDING 266-266 BINDING 309-309

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQDQYILALDQGTTSSRAMLFDRQGNIVSIAQKEFEQIYPQPGWVEHDPQEIWSTQAGVA
CCCCEEEEEECCCCCCEEEEEECCCCEEEHHHHHHHHHCCCCCCCCCCHHHHHCCCCCHH
AEAVTRVGLNGTAIAAIGITNQRETTIVWDRETGHPIYNAIVWQDRRTADFCDKLKAQGL
HHHHHHCCCCCCEEEEEEECCCCCEEEEEECCCCCCHHEEEEECCCCCHHHHHHHHHCCC
SEKVRAKTGLPIDSYFSATKIRWILDNVEGAREKARQGKLAFGTVDSWLVWNFTKHELHV
HHHHHHHCCCCHHHHHHHHEEEEEHHCCHHHHHHHHCCCEEEECCCCEEEEEEECCEEEE
TDVTNASRTMLFNIHTLDWDDELLDALEIPRSMLPQVRASSEIYGPTKTTVFASKIPLAG
EECCCCCEEEEEEEEECCCCHHHHHHHHCCHHHHHHHHCCCCCCCCCCCEEEECCCCEEE
IAGDQQAALFGQMCTSSGMVKNTYGTGCFLMMNTGETPIESRNNLVTTIAWQVDGKVSYA
CCCCCHHHHHHHHHHCCCCEEECCCCEEEEEEECCCCCCCCCCCEEEEEEEEECCEEEEE
LEGSIFIAGAVVQWLRDGLGIIKSASEIEALAAGVPHTDGVYLVPAFAGLGAPHWNARAR
ECCCEEEHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCEEEEEEHHCCCCCCCCCCCC
GSLFGVTRGTTSAHLARAALDSIAYQSLDVLKAMEADSGIRIGELRVDGGASANNLLMQF
CCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHH
QADLLGVDAVRPRITETTALGAAYLAGLAIGYWQNVDELHSQWQLERRFAPSMQAEQVGS
HHHHHCCHHCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCHHHHHH
CLAGWQRAVRAAKAWADDTQ
HHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MQDQYILALDQGTTSSRAMLFDRQGNIVSIAQKEFEQIYPQPGWVEHDPQEIWSTQAGVA
CCCCEEEEEECCCCCCEEEEEECCCCEEEHHHHHHHHHCCCCCCCCCCHHHHHCCCCCHH
AEAVTRVGLNGTAIAAIGITNQRETTIVWDRETGHPIYNAIVWQDRRTADFCDKLKAQGL
HHHHHHCCCCCCEEEEEEECCCCCEEEEEECCCCCCHHEEEEECCCCCHHHHHHHHHCCC
SEKVRAKTGLPIDSYFSATKIRWILDNVEGAREKARQGKLAFGTVDSWLVWNFTKHELHV
HHHHHHHCCCCHHHHHHHHEEEEEHHCCHHHHHHHHCCCEEEECCCCEEEEEEECCEEEE
TDVTNASRTMLFNIHTLDWDDELLDALEIPRSMLPQVRASSEIYGPTKTTVFASKIPLAG
EECCCCCEEEEEEEEECCCCHHHHHHHHCCHHHHHHHHCCCCCCCCCCCEEEECCCCEEE
IAGDQQAALFGQMCTSSGMVKNTYGTGCFLMMNTGETPIESRNNLVTTIAWQVDGKVSYA
CCCCCHHHHHHHHHHCCCCEEECCCCEEEEEEECCCCCCCCCCCEEEEEEEEECCEEEEE
LEGSIFIAGAVVQWLRDGLGIIKSASEIEALAAGVPHTDGVYLVPAFAGLGAPHWNARAR
ECCCEEEHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCEEEEEEHHCCCCCCCCCCCC
GSLFGVTRGTTSAHLARAALDSIAYQSLDVLKAMEADSGIRIGELRVDGGASANNLLMQF
CCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHH
QADLLGVDAVRPRITETTALGAAYLAGLAIGYWQNVDELHSQWQLERRFAPSMQAEQVGS
HHHHHCCHHCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCHHHHHH
CLAGWQRAVRAAKAWADDTQ
HHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA