Click here to switch to the map view.

The map label for this gene is glpF [H]

Identifier: 53718329

GI number: 53718329

Start: 782049

End: 782765

Strand: Reverse

Name: glpF [H]

Synonym: BPSL0686

Alternate gene names: 53718329

Gene position: 782765-782049 (Counterclockwise)

Preceding gene: 53718330

Following gene: 53718328

Centisome position: 19.21

GC content: 66.95

Gene sequence:

>717_bases
ATGTCACCATACATCGCGGAGTTCATCGGCACGGCGCTGCTCGTGCTGCTCGGCAACGGCGCCGTTGCGAACGTGCTGCT
CGCGAAAACCAAAGGCAAGGGCGCGGACCTCATCGTCATCGTGATGGGTTGGGCGATGGCGGTATTCGTCGCGGTCTACG
TAACCGCATCGTTCTCCGGCGCGCACCTGAATCCGATCGTCACGATCAGCCTCGCGCTCGCGGGCAAGTTCGCATGGGCG
AAGGTGGGCGGCTACGTCGCATCGCAGATGCTCGGCGGCATGGCGGGCGCGTTCCTCGTGTGGCTCGCGTATCGCCAGCA
CTTCGCGAAGGAGGCCGATCCCGATCTGAAGCTCGCCGTGTTCTGCACCGCGCCCGCGATCCGCAGCGTCACGCACAACG
TGTTGACGGAGGCGATCTGCACTTTCGTGCTGATCCTCGGCGTGCTGTATCTCGCGTCGCCGCAAGTCGGCCTGGGCGCG
CTCGACGCGCTGCCCGTCGGCCTGCTCGTACTCGGCATCGGCATCTCGCTCGGCGGCCCGACGGGCTACGCGATGAGCCC
CGCGCGCGACCTGTCGCCGCGCATCATGCACGCGCTGCTGCCGATTCCCGGCAAGCGCGATAGCGACTGGCGCTATGCAT
GGGTGCCGGTGCTCGGGCCGCTCGTCGGCGGCGTGCTCGCGGCGAACCTGTACCTGTATCTGCATACGACGCACTGA

Upstream 100 bases:

>100_bases
GGCATCCGTATCGATCACATTTCAAACACATATTCACAACAATCAGGCGGGCCGCGCGACGCAAGCCGCGAGCCCGCCAT
ACCGGTAGAGAGACGACATC

Downstream 100 bases:

>100_bases
ACACGCTGCAGCGGCGCGCCGCACGCCGCGCCGCCGAAATTTCCGCCGCTCGCCGGCGGCCCCCACCCCCCTCGCCCGGC
GACGCGCGAGGGGGTTTTCT

Product: putative glycerol uptake facilitator protein

Products: glycerol [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 238; Mature: 237

Protein sequence:

>238_residues
MSPYIAEFIGTALLVLLGNGAVANVLLAKTKGKGADLIVIVMGWAMAVFVAVYVTASFSGAHLNPIVTISLALAGKFAWA
KVGGYVASQMLGGMAGAFLVWLAYRQHFAKEADPDLKLAVFCTAPAIRSVTHNVLTEAICTFVLILGVLYLASPQVGLGA
LDALPVGLLVLGIGISLGGPTGYAMSPARDLSPRIMHALLPIPGKRDSDWRYAWVPVLGPLVGGVLAANLYLYLHTTH

Sequences:

>Translated_238_residues
MSPYIAEFIGTALLVLLGNGAVANVLLAKTKGKGADLIVIVMGWAMAVFVAVYVTASFSGAHLNPIVTISLALAGKFAWA
KVGGYVASQMLGGMAGAFLVWLAYRQHFAKEADPDLKLAVFCTAPAIRSVTHNVLTEAICTFVLILGVLYLASPQVGLGA
LDALPVGLLVLGIGISLGGPTGYAMSPARDLSPRIMHALLPIPGKRDSDWRYAWVPVLGPLVGGVLAANLYLYLHTTH
>Mature_237_residues
SPYIAEFIGTALLVLLGNGAVANVLLAKTKGKGADLIVIVMGWAMAVFVAVYVTASFSGAHLNPIVTISLALAGKFAWAK
VGGYVASQMLGGMAGAFLVWLAYRQHFAKEADPDLKLAVFCTAPAIRSVTHNVLTEAICTFVLILGVLYLASPQVGLGAL
DALPVGLLVLGIGISLGGPTGYAMSPARDLSPRIMHALLPIPGKRDSDWRYAWVPVLGPLVGGVLAANLYLYLHTTH

Specific function: Glycerol enters the cell via the glycerol diffusion facilitator protein. This membrane protein facilitates the movement of glycerol across the cytoplasmic membrane [H]

COG id: COG0580

COG function: function code G; Glycerol uptake facilitator and related permeases (Major Intrinsic Protein Family)

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MIP/aquaporin (TC 1.A.8) family [H]

Homologues:

Organism=Homo sapiens, GI157266307, Length=248, Percent_Identity=33.8709677419355, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI4826645, Length=245, Percent_Identity=32.2448979591837, Blast_Score=105, Evalue=4e-23,
Organism=Homo sapiens, GI22538420, Length=248, Percent_Identity=31.0483870967742, Blast_Score=101, Evalue=8e-22,
Organism=Homo sapiens, GI4502187, Length=241, Percent_Identity=33.195020746888, Blast_Score=96, Evalue=4e-20,
Organism=Homo sapiens, GI4502179, Length=235, Percent_Identity=30.6382978723404, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI4755125, Length=235, Percent_Identity=25.9574468085106, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI4502181, Length=235, Percent_Identity=25.9574468085106, Blast_Score=67, Evalue=1e-11,
Organism=Escherichia coli, GI1790362, Length=252, Percent_Identity=35.3174603174603, Blast_Score=102, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI71994009, Length=243, Percent_Identity=31.2757201646091, Blast_Score=103, Evalue=7e-23,
Organism=Caenorhabditis elegans, GI17544068, Length=248, Percent_Identity=31.0483870967742, Blast_Score=97, Evalue=9e-21,
Organism=Caenorhabditis elegans, GI17531431, Length=243, Percent_Identity=27.1604938271605, Blast_Score=85, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17531429, Length=243, Percent_Identity=27.1604938271605, Blast_Score=85, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17558372, Length=238, Percent_Identity=30.672268907563, Blast_Score=80, Evalue=8e-16,
Organism=Caenorhabditis elegans, GI71992966, Length=239, Percent_Identity=25.9414225941423, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI71993722, Length=234, Percent_Identity=29.4871794871795, Blast_Score=65, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6321054, Length=211, Percent_Identity=31.7535545023697, Blast_Score=93, Evalue=4e-20,
Organism=Saccharomyces cerevisiae, GI6322985, Length=279, Percent_Identity=26.5232974910394, Blast_Score=79, Evalue=8e-16,
Organism=Drosophila melanogaster, GI19922828, Length=231, Percent_Identity=31.1688311688312, Blast_Score=68, Evalue=6e-12,
Organism=Drosophila melanogaster, GI24762342, Length=236, Percent_Identity=26.271186440678, Blast_Score=64, Evalue=8e-11,
Organism=Drosophila melanogaster, GI24652747, Length=232, Percent_Identity=26.2931034482759, Blast_Score=64, Evalue=8e-11,
Organism=Drosophila melanogaster, GI45551084, Length=232, Percent_Identity=26.2931034482759, Blast_Score=64, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012269
- InterPro:   IPR000425
- InterPro:   IPR022357 [H]

Pfam domain/function: PF00230 MIP [H]

EC number: NA

Molecular weight: Translated: 24909; Mature: 24778

Theoretical pI: Translated: 9.29; Mature: 9.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPYIAEFIGTALLVLLGNGAVANVLLAKTKGKGADLIVIVMGWAMAVFVAVYVTASFSG
CCCHHHHHHHHHHHHHHCCCHHHHHEEEECCCCCCCEEEHHHHHHHHHHHHHHHHHCCCC
AHLNPIVTISLALAGKFAWAKVGGYVASQMLGGMAGAFLVWLAYRQHFAKEADPDLKLAV
CCCCHHEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEE
FCTAPAIRSVTHNVLTEAICTFVLILGVLYLASPQVGLGALDALPVGLLVLGIGISLGGP
EECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCC
TGYAMSPARDLSPRIMHALLPIPGKRDSDWRYAWVPVLGPLVGGVLAANLYLYLHTTH
CCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCEEHHHHHHHHHHHHHHHEEEEEEEECC
>Mature Secondary Structure 
SPYIAEFIGTALLVLLGNGAVANVLLAKTKGKGADLIVIVMGWAMAVFVAVYVTASFSG
CCHHHHHHHHHHHHHHCCCHHHHHEEEECCCCCCCEEEHHHHHHHHHHHHHHHHHCCCC
AHLNPIVTISLALAGKFAWAKVGGYVASQMLGGMAGAFLVWLAYRQHFAKEADPDLKLAV
CCCCHHEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEE
FCTAPAIRSVTHNVLTEAICTFVLILGVLYLASPQVGLGALDALPVGLLVLGIGISLGGP
EECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCC
TGYAMSPARDLSPRIMHALLPIPGKRDSDWRYAWVPVLGPLVGGVLAANLYLYLHTTH
CCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCEEHHHHHHHHHHHHHHHEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: glycerol [Periplasm] [C]

Specific reaction: glycerol [Periplasm] = glycerol [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 10360571 [H]