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The map label for this gene is miaB [H]

Identifier: 53718317

GI number: 53718317

Start: 767263

End: 768636

Strand: Reverse

Name: miaB [H]

Synonym: BPSL0674

Alternate gene names: 53718317

Gene position: 768636-767263 (Counterclockwise)

Preceding gene: 53718318

Following gene: 53718316

Centisome position: 18.86

GC content: 65.28

Gene sequence:

>1374_bases
ATGACCAAGAAAGTTTACGTAAAAACCTTCGGCTGCCAGATGAACGAGTACGACTCCGACAAGATGGTCGACGTGCTCAA
TGCGGCCGAGGGCCTCGAGAAAACCGACACCCCGGAAGACGCGGACATCATCCTGTTCAACACCTGCTCGGTGCGAGAAA
AGGCGCAGGAGAAGGTGTTCTCCGATCTCGGCCGCGTGCGCGAGCTGAAGGAAGCGAAGCCGGACCTGCTGATCGGCGTC
GGCGGCTGCGTGGCGAGCCAGGAAGGCGCGTCGATCGTCGCGCGCGCGCCGTACGTCGATCTCGTGTTCGGCCCGCAAAC
GCTGCACCGCCTGCCGCAGATGATCGACGCGCGCCGCGAAAGCGGCCGCGCGCAGGTCGACATCACGTTCCCCGAAATCG
AGAAGTTCGACCATCTGCCGCCCGCTCGCGTCGAAGGGCCGAGCGCGTTCGTGTCGATCATGGAAGGCTGCTCGAAGTAC
TGCAGCTACTGCGTCGTGCCGTACACGCGCGGCGATGAAGTGTCGCGCCCGCTCGACGACGTCTTGACCGAGGTCGCGGG
CCTCGCCGACCAGGGCGTGCGCGAAGTCACGCTGCTCGGCCAGAACGTGAACGCCTACCGCGGCGCGATCGCGGCCGGCT
CGGCCGAGATCGCCGATTTCGCGACGCTGATCGAATATGTCGCCGACATCCCCGGCATCGAGCGGATCCGCTACACGACA
TCGCATCCGAAGGAGTTCACGCAGCGCCTGCTCGACGTCTACGCGAAGGTGCCGAAGCTCGTCGACCATCTGCACCTGCC
CGTCCAGCACGGCTCGGACCGCATCCTGATGGCGATGAAGCGCGGCTACACCGTGCTCGAATACAAATCGGTGATCCGCA
AGCTGCGCGCGATCCGCCCGAATCTGTCGCTGTCCACGGACATCATCGTGGGTTTCCCCGGCGAGACCGACGCCGACTTC
GACAAGACGATGGCGCTCGTTCACGAGATGAGCTACGACACGAGCTTCTCGTTCATCTACAGCCCGCGGCCCGGCACGCC
GGCCGCGAATCTCGCCGACGACACGCCGCGCGAGCTCAAGCTCAAGCGCCTGCAACATCTGCAGGCGACGATCGAGGAGA
ACGTCGCGAGGATCAGCCAGTCGATGCTCGGCAAGGTCGAGCGAATCCTCGTCGAGGGGCCGTCGCGCAAGGATCCGAAC
GAGCTCGCGGGCCGCACCGAGAACAACCGGGTCGTGAATTTTCCCGCGCCGAGCGCCGCGCACCCGCGCCTGATCGGCCA
GATGATCGACGTGAAGATCAATCACGCGTATCCCCACTCGCTGCGCGGCGAGCTCGTGCTCGCGCACGGCGACGCGAGCG
CCGCCACGCACTGA

Upstream 100 bases:

>100_bases
GTACGCGGCGCGCATCGCGCGGCTGGCGGCGCGCCGATCCGTCCCCTGACTACACTTTGAACGACGCCCCCAGGTCAACC
ACTGCGAACGGCGAACCCTC

Downstream 100 bases:

>100_bases
CGCAACAGGAACCCGACGCCACTTTGAAAACCGCTCAAGCACTGGAATTCACCGCGCCGCGCGACGACAACGCGCGCCTC
GCGAACCTCTGCGGCCCGCT

Product: (dimethylallyl)adenosine tRNA methylthiotransferase

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase [H]

Number of amino acids: Translated: 457; Mature: 456

Protein sequence:

>457_residues
MTKKVYVKTFGCQMNEYDSDKMVDVLNAAEGLEKTDTPEDADIILFNTCSVREKAQEKVFSDLGRVRELKEAKPDLLIGV
GGCVASQEGASIVARAPYVDLVFGPQTLHRLPQMIDARRESGRAQVDITFPEIEKFDHLPPARVEGPSAFVSIMEGCSKY
CSYCVVPYTRGDEVSRPLDDVLTEVAGLADQGVREVTLLGQNVNAYRGAIAAGSAEIADFATLIEYVADIPGIERIRYTT
SHPKEFTQRLLDVYAKVPKLVDHLHLPVQHGSDRILMAMKRGYTVLEYKSVIRKLRAIRPNLSLSTDIIVGFPGETDADF
DKTMALVHEMSYDTSFSFIYSPRPGTPAANLADDTPRELKLKRLQHLQATIEENVARISQSMLGKVERILVEGPSRKDPN
ELAGRTENNRVVNFPAPSAAHPRLIGQMIDVKINHAYPHSLRGELVLAHGDASAATH

Sequences:

>Translated_457_residues
MTKKVYVKTFGCQMNEYDSDKMVDVLNAAEGLEKTDTPEDADIILFNTCSVREKAQEKVFSDLGRVRELKEAKPDLLIGV
GGCVASQEGASIVARAPYVDLVFGPQTLHRLPQMIDARRESGRAQVDITFPEIEKFDHLPPARVEGPSAFVSIMEGCSKY
CSYCVVPYTRGDEVSRPLDDVLTEVAGLADQGVREVTLLGQNVNAYRGAIAAGSAEIADFATLIEYVADIPGIERIRYTT
SHPKEFTQRLLDVYAKVPKLVDHLHLPVQHGSDRILMAMKRGYTVLEYKSVIRKLRAIRPNLSLSTDIIVGFPGETDADF
DKTMALVHEMSYDTSFSFIYSPRPGTPAANLADDTPRELKLKRLQHLQATIEENVARISQSMLGKVERILVEGPSRKDPN
ELAGRTENNRVVNFPAPSAAHPRLIGQMIDVKINHAYPHSLRGELVLAHGDASAATH
>Mature_456_residues
TKKVYVKTFGCQMNEYDSDKMVDVLNAAEGLEKTDTPEDADIILFNTCSVREKAQEKVFSDLGRVRELKEAKPDLLIGVG
GCVASQEGASIVARAPYVDLVFGPQTLHRLPQMIDARRESGRAQVDITFPEIEKFDHLPPARVEGPSAFVSIMEGCSKYC
SYCVVPYTRGDEVSRPLDDVLTEVAGLADQGVREVTLLGQNVNAYRGAIAAGSAEIADFATLIEYVADIPGIERIRYTTS
HPKEFTQRLLDVYAKVPKLVDHLHLPVQHGSDRILMAMKRGYTVLEYKSVIRKLRAIRPNLSLSTDIIVGFPGETDADFD
KTMALVHEMSYDTSFSFIYSPRPGTPAANLADDTPRELKLKRLQHLQATIEENVARISQSMLGKVERILVEGPSRKDPNE
LAGRTENNRVVNFPAPSAAHPRLIGQMIDVKINHAYPHSLRGELVLAHGDASAATH

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine [H]

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI28872784, Length=478, Percent_Identity=36.6108786610879, Blast_Score=301, Evalue=6e-82,
Organism=Homo sapiens, GI28872782, Length=478, Percent_Identity=36.6108786610879, Blast_Score=301, Evalue=9e-82,
Organism=Homo sapiens, GI93277076, Length=466, Percent_Identity=28.3261802575107, Blast_Score=176, Evalue=3e-44,
Organism=Escherichia coli, GI1786882, Length=446, Percent_Identity=61.8834080717489, Blast_Score=571, Evalue=1e-164,
Organism=Escherichia coli, GI1787057, Length=461, Percent_Identity=28.4164859002169, Blast_Score=160, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI17553146, Length=464, Percent_Identity=34.4827586206897, Blast_Score=269, Evalue=3e-72,
Organism=Caenorhabditis elegans, GI71996771, Length=257, Percent_Identity=31.1284046692607, Blast_Score=118, Evalue=7e-27,
Organism=Drosophila melanogaster, GI21356207, Length=475, Percent_Identity=33.2631578947368, Blast_Score=285, Evalue=6e-77,
Organism=Drosophila melanogaster, GI19922432, Length=459, Percent_Identity=29.1938997821351, Blast_Score=157, Evalue=2e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792 [H]

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004 [H]

EC number: NA

Molecular weight: Translated: 50512; Mature: 50381

Theoretical pI: Translated: 6.20; Mature: 6.20

Prosite motif: PS50926 TRAM ; PS01278 UPF0004

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKKVYVKTFGCQMNEYDSDKMVDVLNAAEGLEKTDTPEDADIILFNTCSVREKAQEKVF
CCCEEEEEEECCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCHHHHHHHHHH
SDLGRVRELKEAKPDLLIGVGGCVASQEGASIVARAPYVDLVFGPQTLHRLPQMIDARRE
HHHHHHHHHHHCCCCEEEECCCCCCCCCCCEEEEECCEEEEEECHHHHHHHHHHHHHHHH
SGRAQVDITFPEIEKFDHLPPARVEGPSAFVSIMEGCSKYCSYCVVPYTRGDEVSRPLDD
CCCEEEEEECHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCEEEEECCCCCCCCCCHHH
VLTEVAGLADQGVREVTLLGQNVNAYRGAIAAGSAEIADFATLIEYVADIPGIERIRYTT
HHHHHHHHHHCCCCEEEEECCCCHHHHCHHHCCCHHHHHHHHHHHHHHCCCCCCEEEECC
SHPKEFTQRLLDVYAKVPKLVDHLHLPVQHGSDRILMAMKRGYTVLEYKSVIRKLRAIRP
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEHHHCCCCHHHHHHHHHHHHHHCC
NLSLSTDIIVGFPGETDADFDKTMALVHEMSYDTSFSFIYSPRPGTPAANLADDTPRELK
CCCCCCEEEEECCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCHHHHH
LKRLQHLQATIEENVARISQSMLGKVERILVEGPSRKDPNELAGRTENNRVVNFPAPSAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCCCEEEECCCCCCC
HPRLIGQMIDVKINHAYPHSLRGELVLAHGDASAATH
CHHHHHHHHEEEEECCCCCCCCCCEEEEECCCCCCCC
>Mature Secondary Structure 
TKKVYVKTFGCQMNEYDSDKMVDVLNAAEGLEKTDTPEDADIILFNTCSVREKAQEKVF
CCEEEEEEECCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCHHHHHHHHHH
SDLGRVRELKEAKPDLLIGVGGCVASQEGASIVARAPYVDLVFGPQTLHRLPQMIDARRE
HHHHHHHHHHHCCCCEEEECCCCCCCCCCCEEEEECCEEEEEECHHHHHHHHHHHHHHHH
SGRAQVDITFPEIEKFDHLPPARVEGPSAFVSIMEGCSKYCSYCVVPYTRGDEVSRPLDD
CCCEEEEEECHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCEEEEECCCCCCCCCCHHH
VLTEVAGLADQGVREVTLLGQNVNAYRGAIAAGSAEIADFATLIEYVADIPGIERIRYTT
HHHHHHHHHHCCCCEEEEECCCCHHHHCHHHCCCHHHHHHHHHHHHHHCCCCCCEEEECC
SHPKEFTQRLLDVYAKVPKLVDHLHLPVQHGSDRILMAMKRGYTVLEYKSVIRKLRAIRP
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEHHHCCCCHHHHHHHHHHHHHHCC
NLSLSTDIIVGFPGETDADFDKTMALVHEMSYDTSFSFIYSPRPGTPAANLADDTPRELK
CCCCCCEEEEECCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCHHHHH
LKRLQHLQATIEENVARISQSMLGKVERILVEGPSRKDPNELAGRTENNRVVNFPAPSAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCCCEEEECCCCCCC
HPRLIGQMIDVKINHAYPHSLRGELVLAHGDASAATH
CHHHHHHHHEEEEECCCCCCCCCCEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA