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The map label for this gene is plcN2 [H]

Identifier: 53717979

GI number: 53717979

Start: 359487

End: 361604

Strand: Reverse

Name: plcN2 [H]

Synonym: BPSL0338

Alternate gene names: 53717979

Gene position: 361604-359487 (Counterclockwise)

Preceding gene: 53717980

Following gene: 53717978

Centisome position: 8.87

GC content: 68.08

Gene sequence:

>2118_bases
ATGACATCAGAAAGCCGCCGCCGTTTCCTGCATACCGTCGCGCAATCCGCGGGCGCCGCCGCCGCGCTCACCGTGTTTCC
CGAATCGATTCGCCGCGCACTCGCGATTCCCGCCGCGTCGCGCACGGGCACGCTGCGCGATGTCGAGCACATCGTCGTGT
TCATGCAGGAAAACCGCTCGTTCGACCATTACTTCGGACACCTGCGCGGTGTGCGCGGCTACAACGACCGCTTTCCGATT
CCGCTGCCGAACGGCAAACCGGTCTGGTATCAGCCGTCGAAGGCGAATCCGGGCAAGCCGGTGCTGCCGTTTCGCCTGAA
CACGCTGACGACGAGCGCCCAGTGCATCGGCGATCTCGATCACTCGTGGTACAAGACGCATGCGGCGATCGACGGCGGCC
GCTACGATCAGTGGCCCGCGAACAAGACCGACATGACGATGGGCTACCACGTGCGCGAGGACATCCCGTTCCACTACGCG
CTCGCCGACGCGTTCACCGTCTGCGACAACTACTTCTGCTCGCTGCCGGGCCCGACGCATCCGAACCGCTCGTATCTGAT
GACGGGCACGGTCGATCCGACCGGCCGGTACGGCGGCCCGCTGCTCGACAACAGCGATTACGTGGACGGCGACGTGCCGC
CGAAATACGATCTGCTCACCTGGACGACGTACCCCGAACGCCTCGAGGCGAACGGCATTTCGTGGCAGATCTATCAGCAG
GGCACGACGGGGGCCGATCCGCTGAACGGCAACTACGGGACGAACATCCTGCAGAACTTCGCGAACTTCATCAACGCGAA
GCCGGGCTCGTCGCTGTATCGGCGCGCGCAGACCGCGCGCACGCTCGACGATCTGAAGGCCGACGTGCTCGCGAACAAGC
TGCCGCAGGTGTCGTGGCTGCTGCCGCCCGCGGCGTTCTCCGAGCATCCGAGATACACGCCCGCGTACGGCGCGAACTAC
ACGTCGCAGATCCTCGACGCGCTGACGTCGAACCCCGACGTGTGGCGCAAGACCGTGCTCTTCATCATGTACGACGAGAA
CGACGGCTTCTTCGATCACGTCGTGCCGCCGCAGCCGGCGACGACGCGCGCGCAGGGCCTGTCGACCGTGACCGTCGACG
GCGAGATCCACGACGTCGTGAACCCGGGCCGCGGCGGCAGCTACACGGCCGACGGCCTGCCGTACGGCCTCGGCCCGCGC
GTGCCGATGACGGTCGTGTCGCCGTGGACGAAGGGCGGCTTCGTGTGCTCGCAGGTGTTCGACCATACGTCGGTGATCCG
CTTCATCGCCGCGCGCTTCGGCATCGACGAGCCGAACATCACGCCGTGGCGCCGCGCGGTGTGCGGCGATCTCACGTCGG
CGTTCGACTTCCGCACGCCGGACGCGACGCTGCCGCCGTTGCCCGACACGAGCGGCTATCGGTCGATCGCCGATCAGCTC
TGCTCGACGAAGCCCGCGCCCGCCGTGCCCGCCGCGCCGAGCCCCGTCGATCCGCAGGAGCCCGGCGTGCGCCCGGCGCG
CGCGCTGCCTTACGAGCTGCACGTGAACGCCGAGGTGCACGGCGGCGCGCGCCTGCGCATCGAGTTCGCGAATCGCGGCG
AGCAAGGCGCGCACTTCTATGTGTACGCATCGAATCGCAGCGACGGCCCGTGGCGCTACACGGTCGGCGCGCACCGCTCG
TTGCACGAAACGTTCGATCTCGCCGCGACGAACGGCGCGTACGCGTTCGCCGTGTATGGTCCGAACGGCTTCGTGCGCGT
GTTCGAAGGCCTCGCCGCGGCCGGAGGCCACGGCCGGCAAGCGGCCGCGCGCCCCGAGGTGAAGGCGGGCTACGACGTCG
CGAACGGCAACCTGTATCTGAAGCTCGCGAATCATGGCGCACAGAGCGTGCAACTGACGCTCGCCGACAACGCGTACGGC
GCGCCGGCGCGCCGGATCACGCTGCGCGGCGGCGATGAGCAGATCGAATCGTGGGCGCTGGCGTCGAGCCATCAATGGTA
TGACGTGACGGTGTCGGACGGCGCGACCGGCCAGTTCGTGCGCCGCTTCGCGGGGCACGTCGAGAACGGCAAGCCGAGCT
ATTCCGATCCGGCGGCGATCGCGCCCGTCACGGGCTGA

Upstream 100 bases:

>100_bases
CGACGCATCCGGCGCGGCGGCGGCGTCCGCGCAGAGCGCCTCGCATCCTGGCTGATCGCCTTTGCCGCGCCCTCTTCCCC
GCAGAGCCCCAGGTGAAAAC

Downstream 100 bases:

>100_bases
ACACGGCCGCGCCGGCGGCGGCAACCGTCTGAACGGCGGGGCGACGAACCGGTAGTCCCGCCGCGCCGCCCGGCTTCGAC
GGCACGCATCGCGGCATGCT

Product: non-hemolytic phospholipase C

Products: NA

Alternate protein names: PLC-N; Phosphatidylcholine cholinephosphohydrolase; Phosphatidylcholine-hydrolyzing phospholipase C; PC-PLC [H]

Number of amino acids: Translated: 705; Mature: 704

Protein sequence:

>705_residues
MTSESRRRFLHTVAQSAGAAAALTVFPESIRRALAIPAASRTGTLRDVEHIVVFMQENRSFDHYFGHLRGVRGYNDRFPI
PLPNGKPVWYQPSKANPGKPVLPFRLNTLTTSAQCIGDLDHSWYKTHAAIDGGRYDQWPANKTDMTMGYHVREDIPFHYA
LADAFTVCDNYFCSLPGPTHPNRSYLMTGTVDPTGRYGGPLLDNSDYVDGDVPPKYDLLTWTTYPERLEANGISWQIYQQ
GTTGADPLNGNYGTNILQNFANFINAKPGSSLYRRAQTARTLDDLKADVLANKLPQVSWLLPPAAFSEHPRYTPAYGANY
TSQILDALTSNPDVWRKTVLFIMYDENDGFFDHVVPPQPATTRAQGLSTVTVDGEIHDVVNPGRGGSYTADGLPYGLGPR
VPMTVVSPWTKGGFVCSQVFDHTSVIRFIAARFGIDEPNITPWRRAVCGDLTSAFDFRTPDATLPPLPDTSGYRSIADQL
CSTKPAPAVPAAPSPVDPQEPGVRPARALPYELHVNAEVHGGARLRIEFANRGEQGAHFYVYASNRSDGPWRYTVGAHRS
LHETFDLAATNGAYAFAVYGPNGFVRVFEGLAAAGGHGRQAAARPEVKAGYDVANGNLYLKLANHGAQSVQLTLADNAYG
APARRITLRGGDEQIESWALASSHQWYDVTVSDGATGQFVRRFAGHVENGKPSYSDPAAIAPVTG

Sequences:

>Translated_705_residues
MTSESRRRFLHTVAQSAGAAAALTVFPESIRRALAIPAASRTGTLRDVEHIVVFMQENRSFDHYFGHLRGVRGYNDRFPI
PLPNGKPVWYQPSKANPGKPVLPFRLNTLTTSAQCIGDLDHSWYKTHAAIDGGRYDQWPANKTDMTMGYHVREDIPFHYA
LADAFTVCDNYFCSLPGPTHPNRSYLMTGTVDPTGRYGGPLLDNSDYVDGDVPPKYDLLTWTTYPERLEANGISWQIYQQ
GTTGADPLNGNYGTNILQNFANFINAKPGSSLYRRAQTARTLDDLKADVLANKLPQVSWLLPPAAFSEHPRYTPAYGANY
TSQILDALTSNPDVWRKTVLFIMYDENDGFFDHVVPPQPATTRAQGLSTVTVDGEIHDVVNPGRGGSYTADGLPYGLGPR
VPMTVVSPWTKGGFVCSQVFDHTSVIRFIAARFGIDEPNITPWRRAVCGDLTSAFDFRTPDATLPPLPDTSGYRSIADQL
CSTKPAPAVPAAPSPVDPQEPGVRPARALPYELHVNAEVHGGARLRIEFANRGEQGAHFYVYASNRSDGPWRYTVGAHRS
LHETFDLAATNGAYAFAVYGPNGFVRVFEGLAAAGGHGRQAAARPEVKAGYDVANGNLYLKLANHGAQSVQLTLADNAYG
APARRITLRGGDEQIESWALASSHQWYDVTVSDGATGQFVRRFAGHVENGKPSYSDPAAIAPVTG
>Mature_704_residues
TSESRRRFLHTVAQSAGAAAALTVFPESIRRALAIPAASRTGTLRDVEHIVVFMQENRSFDHYFGHLRGVRGYNDRFPIP
LPNGKPVWYQPSKANPGKPVLPFRLNTLTTSAQCIGDLDHSWYKTHAAIDGGRYDQWPANKTDMTMGYHVREDIPFHYAL
ADAFTVCDNYFCSLPGPTHPNRSYLMTGTVDPTGRYGGPLLDNSDYVDGDVPPKYDLLTWTTYPERLEANGISWQIYQQG
TTGADPLNGNYGTNILQNFANFINAKPGSSLYRRAQTARTLDDLKADVLANKLPQVSWLLPPAAFSEHPRYTPAYGANYT
SQILDALTSNPDVWRKTVLFIMYDENDGFFDHVVPPQPATTRAQGLSTVTVDGEIHDVVNPGRGGSYTADGLPYGLGPRV
PMTVVSPWTKGGFVCSQVFDHTSVIRFIAARFGIDEPNITPWRRAVCGDLTSAFDFRTPDATLPPLPDTSGYRSIADQLC
STKPAPAVPAAPSPVDPQEPGVRPARALPYELHVNAEVHGGARLRIEFANRGEQGAHFYVYASNRSDGPWRYTVGAHRSL
HETFDLAATNGAYAFAVYGPNGFVRVFEGLAAAGGHGRQAAARPEVKAGYDVANGNLYLKLANHGAQSVQLTLADNAYGA
PARRITLRGGDEQIESWALASSHQWYDVTVSDGATGQFVRRFAGHVENGKPSYSDPAAIAPVTG

Specific function: Hydrolyzes phosphatidylserine as well as phosphatidylcholine [H]

COG id: COG3511

COG function: function code M; Phospholipase C

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial phospholipase C family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007312
- InterPro:   IPR008475
- InterPro:   IPR017767
- InterPro:   IPR006311 [H]

Pfam domain/function: PF05506 DUF756; PF04185 Phosphoesterase [H]

EC number: =3.1.4.3 [H]

Molecular weight: Translated: 77022; Mature: 76890

Theoretical pI: Translated: 7.01; Mature: 7.01

Prosite motif: PS00389 ATPASE_DELTA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSESRRRFLHTVAQSAGAAAALTVFPESIRRALAIPAASRTGTLRDVEHIVVFMQENRS
CCCHHHHHHHHHHHHHCCCEEEEEECHHHHHHHHCCCCCCCCCCHHHHEEEEEEEECCCC
FDHYFGHLRGVRGYNDRFPIPLPNGKPVWYQPSKANPGKPVLPFRLNTLTTSAQCIGDLD
HHHHHHHHCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCEEECCCCCHHHHHHCCC
HSWYKTHAAIDGGRYDQWPANKTDMTMGYHVREDIPFHYALADAFTVCDNYFCSLPGPTH
HHHHHHHEEECCCCCCCCCCCCCCEEECEEECCCCCEEHHHHHHHHHHCCCEECCCCCCC
PNRSYLMTGTVDPTGRYGGPLLDNSDYVDGDVPPKYDLLTWTTYPERLEANGISWQIYQQ
CCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCHHHCCCCCEEEEEEC
GTTGADPLNGNYGTNILQNFANFINAKPGSSLYRRAQTARTLDDLKADVLANKLPQVSWL
CCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEE
LPPAAFSEHPRYTPAYGANYTSQILDALTSNPDVWRKTVLFIMYDENDGFFDHVVPPQPA
CCCHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEEEEEEEECCCCCCCCCCCCCCC
TTRAQGLSTVTVDGEIHDVVNPGRGGSYTADGLPYGLGPRVPMTVVSPWTKGGFVCSQVF
CHHHCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCHHHHHH
DHTSVIRFIAARFGIDEPNITPWRRAVCGDLTSAFDFRTPDATLPPLPDTSGYRSIADQL
HHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHH
CSTKPAPAVPAAPSPVDPQEPGVRPARALPYELHVNAEVHGGARLRIEFANRGEQGAHFY
HCCCCCCCCCCCCCCCCCCCCCCCCHHCCCEEEEEEEEECCCEEEEEEECCCCCCCCEEE
VYASNRSDGPWRYTVGAHRSLHETFDLAATNGAYAFAVYGPNGFVRVFEGLAAAGGHGRQ
EEECCCCCCCEEEEECCCHHHHHHHHHEECCCEEEEEEECCCCHHHHHHHHHHCCCCCCC
AAARPEVKAGYDVANGNLYLKLANHGAQSVQLTLADNAYGAPARRITLRGGDEQIESWAL
CCCCCCCCCCCEEECCEEEEEEECCCCCEEEEEEECCCCCCCCEEEEECCCHHHHHHHHH
ASSHQWYDVTVSDGATGQFVRRFAGHVENGKPSYSDPAAIAPVTG
CCCCCEEEEEECCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TSESRRRFLHTVAQSAGAAAALTVFPESIRRALAIPAASRTGTLRDVEHIVVFMQENRS
CCHHHHHHHHHHHHHCCCEEEEEECHHHHHHHHCCCCCCCCCCHHHHEEEEEEEECCCC
FDHYFGHLRGVRGYNDRFPIPLPNGKPVWYQPSKANPGKPVLPFRLNTLTTSAQCIGDLD
HHHHHHHHCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCEEECCCCCHHHHHHCCC
HSWYKTHAAIDGGRYDQWPANKTDMTMGYHVREDIPFHYALADAFTVCDNYFCSLPGPTH
HHHHHHHEEECCCCCCCCCCCCCCEEECEEECCCCCEEHHHHHHHHHHCCCEECCCCCCC
PNRSYLMTGTVDPTGRYGGPLLDNSDYVDGDVPPKYDLLTWTTYPERLEANGISWQIYQQ
CCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCHHHCCCCCEEEEEEC
GTTGADPLNGNYGTNILQNFANFINAKPGSSLYRRAQTARTLDDLKADVLANKLPQVSWL
CCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEE
LPPAAFSEHPRYTPAYGANYTSQILDALTSNPDVWRKTVLFIMYDENDGFFDHVVPPQPA
CCCHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEEEEEEEECCCCCCCCCCCCCCC
TTRAQGLSTVTVDGEIHDVVNPGRGGSYTADGLPYGLGPRVPMTVVSPWTKGGFVCSQVF
CHHHCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCHHHHHH
DHTSVIRFIAARFGIDEPNITPWRRAVCGDLTSAFDFRTPDATLPPLPDTSGYRSIADQL
HHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHH
CSTKPAPAVPAAPSPVDPQEPGVRPARALPYELHVNAEVHGGARLRIEFANRGEQGAHFY
HCCCCCCCCCCCCCCCCCCCCCCCCHHCCCEEEEEEEEECCCEEEEEEECCCCCCCCEEE
VYASNRSDGPWRYTVGAHRSLHETFDLAATNGAYAFAVYGPNGFVRVFEGLAAAGGHGRQ
EEECCCCCCCEEEEECCCHHHHHHHHHEECCCEEEEEEECCCCHHHHHHHHHHCCCCCCC
AAARPEVKAGYDVANGNLYLKLANHGAQSVQLTLADNAYGAPARRITLRGGDEQIESWAL
CCCCCCCCCCCEEECCEEEEEEECCCCCEEEEEEECCCCCCCCEEEEECCCHHHHHHHHH
ASSHQWYDVTVSDGATGQFVRRFAGHVENGKPSYSDPAAIAPVTG
CCCCCEEEEEECCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10523590 [H]