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The map label for this gene is ghrA [H]

Identifier: 53717975

GI number: 53717975

Start: 356748

End: 357689

Strand: Reverse

Name: ghrA [H]

Synonym: BPSL0334

Alternate gene names: 53717975

Gene position: 357689-356748 (Counterclockwise)

Preceding gene: 53717976

Following gene: 53717974

Centisome position: 8.78

GC content: 73.14

Gene sequence:

>942_bases
ATGCGCATTCTGCTCTACACGCCGCATCGGGACGCGCACGACTGGAAGCGCGACGTCGAGCGCGCGCTGCCCGGCGCGCA
GTTGCGCACGTGGACGCCCGGCGACGACGCAAGCGCCGATTACGCGCTCGTCTGGCGCGCGCCGCGCGAGTTCTTCGCGC
CGCGCGCGGGTTTGAAGGCGGTGTTCAATCTCGGCGCGGGCGTCGATGCGATACTCGCGCTCGAGCGCGAGCATCCGGGC
CTGCTGCCGCGCGACGTGCCGCTCGTGCGCCTCGAGGACGCCGGGATGGCGCGGCAGATGGTCGAGTACGCGACGCACGC
GGCGCTGCGCTATCTGCGCCGCTTCGACGAATACGCGCTGCTGCAGCGCGAGCGCCGCTGGCAGGTGCTCGCGCCGCATG
CGCGCGAAACCTTCGTCGTCGGCGTGCTCGGGCTCGGCGCGCTCGGCGCCGAAGTCGCGCGCGCGCTCGCCGCGCTCGGG
CTGCCGGTGCGCGGCTACAGCCGCGCGCCGAAGGCGATCGACGGTGTCACGACGTTCGCGGGCGCCGCGCAGCTCGACGC
GTTCCTCGGCGGCGCGAAGCTGCTCGTGAACCTGCTGCCGAGCACGGCGGCTACCGACGGCATCCTGAACGCGCGCACGT
TCTCGAAGCTCGCGCACGGCGCGTATCTCGTGAACCTCGCGCGCGGCGCGCACCTCGTCGAAGCGGATCTGCTCGACGCG
CTCGCGAGCGGCCGCGTCGCCGCGGCGACGCTCGACGTGTTCGCGACCGAACCGCTGCCGCCCGATCACCCGTTCTGGCA
CGAGCCGCGCATCACGATCACGCCGCACTGCTCGGCCGATACGCTGCGCGCGGAGGCGGTCGGGCAGATCGCGGCGAAGA
TCGGCGCGCTCGAACGCGGCGAGCCGATCGGCGGCATCGTCGATCGCGATCGCGGCTACTGA

Upstream 100 bases:

>100_bases
GCGGGCGAGCAGGCGACGATGTTCTTCGTCGATCCATGCGGCAACGCGCTGGAATTCAAGGCTTTCGCCGACATCGGCCA
GTTGTTCGCGAAGTAGACGC

Downstream 100 bases:

>100_bases
CCGCGGCCCGCGAACGCGCGCCGCACGAGCCCATGCGCCGCAACAGCCGTTACGTTCATCAGAAAACAGGAATCAGGAGA
CTCGGATGACGCTCCCCCAA

Product: hypothetical protein

Products: NA

Alternate protein names: 2-ketoacid reductase [H]

Number of amino acids: Translated: 313; Mature: 313

Protein sequence:

>313_residues
MRILLYTPHRDAHDWKRDVERALPGAQLRTWTPGDDASADYALVWRAPREFFAPRAGLKAVFNLGAGVDAILALEREHPG
LLPRDVPLVRLEDAGMARQMVEYATHAALRYLRRFDEYALLQRERRWQVLAPHARETFVVGVLGLGALGAEVARALAALG
LPVRGYSRAPKAIDGVTTFAGAAQLDAFLGGAKLLVNLLPSTAATDGILNARTFSKLAHGAYLVNLARGAHLVEADLLDA
LASGRVAAATLDVFATEPLPPDHPFWHEPRITITPHCSADTLRAEAVGQIAAKIGALERGEPIGGIVDRDRGY

Sequences:

>Translated_313_residues
MRILLYTPHRDAHDWKRDVERALPGAQLRTWTPGDDASADYALVWRAPREFFAPRAGLKAVFNLGAGVDAILALEREHPG
LLPRDVPLVRLEDAGMARQMVEYATHAALRYLRRFDEYALLQRERRWQVLAPHARETFVVGVLGLGALGAEVARALAALG
LPVRGYSRAPKAIDGVTTFAGAAQLDAFLGGAKLLVNLLPSTAATDGILNARTFSKLAHGAYLVNLARGAHLVEADLLDA
LASGRVAAATLDVFATEPLPPDHPFWHEPRITITPHCSADTLRAEAVGQIAAKIGALERGEPIGGIVDRDRGY
>Mature_313_residues
MRILLYTPHRDAHDWKRDVERALPGAQLRTWTPGDDASADYALVWRAPREFFAPRAGLKAVFNLGAGVDAILALEREHPG
LLPRDVPLVRLEDAGMARQMVEYATHAALRYLRRFDEYALLQRERRWQVLAPHARETFVVGVLGLGALGAEVARALAALG
LPVRGYSRAPKAIDGVTTFAGAAQLDAFLGGAKLLVNLLPSTAATDGILNARTFSKLAHGAYLVNLARGAHLVEADLLDA
LASGRVAAATLDVFATEPLPPDHPFWHEPRITITPHCSADTLRAEAVGQIAAKIGALERGEPIGGIVDRDRGY

Specific function: Catalyzes the NADPH-dependent reduction of glyoxylate and hydroxypyruvate into glycolate and glycerate, respectively [H]

COG id: COG0111

COG function: function code HE; Phosphoglycerate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. GhrA subfamily [H]

Homologues:

Organism=Homo sapiens, GI6912396, Length=256, Percent_Identity=28.515625, Blast_Score=89, Evalue=5e-18,
Organism=Homo sapiens, GI23308577, Length=263, Percent_Identity=27.7566539923954, Blast_Score=81, Evalue=1e-15,
Organism=Escherichia coli, GI87081824, Length=313, Percent_Identity=51.4376996805112, Blast_Score=313, Evalue=8e-87,
Organism=Escherichia coli, GI87082289, Length=148, Percent_Identity=29.7297297297297, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17532191, Length=272, Percent_Identity=27.2058823529412, Blast_Score=78, Evalue=5e-15,
Organism=Saccharomyces cerevisiae, GI6322116, Length=197, Percent_Identity=24.8730964467005, Blast_Score=64, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6320925, Length=197, Percent_Identity=24.8730964467005, Blast_Score=63, Evalue=5e-11,
Organism=Drosophila melanogaster, GI28574286, Length=235, Percent_Identity=28.5106382978723, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24585514, Length=238, Percent_Identity=27.3109243697479, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI28574282, Length=238, Percent_Identity=27.3109243697479, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI45552429, Length=238, Percent_Identity=27.3109243697479, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI28574284, Length=238, Percent_Identity=27.3109243697479, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI45551003, Length=238, Percent_Identity=27.3109243697479, Blast_Score=79, Evalue=4e-15,
Organism=Drosophila melanogaster, GI24585516, Length=169, Percent_Identity=30.7692307692308, Blast_Score=75, Evalue=7e-14,
Organism=Drosophila melanogaster, GI28571528, Length=156, Percent_Identity=32.0512820512821, Blast_Score=73, Evalue=3e-13,
Organism=Drosophila melanogaster, GI19921140, Length=174, Percent_Identity=31.6091954022989, Blast_Score=68, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02826 2-Hacid_dh_C [H]

EC number: =1.1.1.79; =1.1.1.81 [H]

Molecular weight: Translated: 33871; Mature: 33871

Theoretical pI: Translated: 7.88; Mature: 7.88

Prosite motif: PS00065 D_2_HYDROXYACID_DH_1 ; PS00671 D_2_HYDROXYACID_DH_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRILLYTPHRDAHDWKRDVERALPGAQLRTWTPGDDASADYALVWRAPREFFAPRAGLKA
CEEEEECCCCCHHHHHHHHHHHCCCCCEEECCCCCCCCCCEEEEEECCHHHHCCCHHHHH
VFNLGAGVDAILALEREHPGLLPRDVPLVRLEDAGMARQMVEYATHAALRYLRRFDEYAL
HHHCCCCHHHHEEEECCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LQRERRWQVLAPHARETFVVGVLGLGALGAEVARALAALGLPVRGYSRAPKAIDGVTTFA
HHHHCCCCEECCCCCCEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHH
GAAQLDAFLGGAKLLVNLLPSTAATDGILNARTFSKLAHGAYLVNLARGAHLVEADLLDA
HHHHHHHHHHHHHHHHHHHCCHHHHCCCHHHHHHHHHHHHHHEEEHHCCCHHHHHHHHHH
LASGRVAAATLDVFATEPLPPDHPFWHEPRITITPHCSADTLRAEAVGQIAAKIGALERG
HHCCCEEEEEEEHHCCCCCCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHCCCCCC
EPIGGIVDRDRGY
CCCCCCCCCCCCC
>Mature Secondary Structure
MRILLYTPHRDAHDWKRDVERALPGAQLRTWTPGDDASADYALVWRAPREFFAPRAGLKA
CEEEEECCCCCHHHHHHHHHHHCCCCCEEECCCCCCCCCCEEEEEECCHHHHCCCHHHHH
VFNLGAGVDAILALEREHPGLLPRDVPLVRLEDAGMARQMVEYATHAALRYLRRFDEYAL
HHHCCCCHHHHEEEECCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LQRERRWQVLAPHARETFVVGVLGLGALGAEVARALAALGLPVRGYSRAPKAIDGVTTFA
HHHHCCCCEECCCCCCEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHH
GAAQLDAFLGGAKLLVNLLPSTAATDGILNARTFSKLAHGAYLVNLARGAHLVEADLLDA
HHHHHHHHHHHHHHHHHHHCCHHHHCCCHHHHHHHHHHHHHHEEEHHCCCHHHHHHHHHH
LASGRVAAATLDVFATEPLPPDHPFWHEPRITITPHCSADTLRAEAVGQIAAKIGALERG
HHCCCEEEEEEEHHCCCCCCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHCCCCCC
EPIGGIVDRDRGY
CCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA