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The map label for this gene is purR [H]

Identifier: 53717963

GI number: 53717963

Start: 342488

End: 343507

Strand: Reverse

Name: purR [H]

Synonym: BPSL0322

Alternate gene names: 53717963

Gene position: 343507-342488 (Counterclockwise)

Preceding gene: 53717964

Following gene: 53717962

Centisome position: 8.43

GC content: 71.76

Gene sequence:

>1020_bases
ATGGGCACCACCATTCGCGACGTTGCGCAGGCGGCCAACGTGTCGATCGGCACCGTGTCGCGCGCGCTCAAGAACCAGCC
CGGTCTGTCCGAGGCGACGCGCGCGCGCATCGTCGAGATCGCGCACCGCATGAACTACGATCCGACGCAACTGCGTCCGC
GGATCAAGCGGCTCACGTTCCTGCTGCATCGCCAGCACAACAACTTCGCGACGACGCCGTTCTTCTCGCATGTGCTGCAC
GGCGTCGAGGACGCGTGCCGCGAGCGCGGCATCGTGCCGTCGCTGCTGACGACGGGCCCGACCGACGACGTGATCCGCCA
GATGCGCCCGCACGCGCCCGACGCGATCGCGGTCGCGGGCTTCATGGAGCCGGAGACGCTCGAGGCGCTCGCGGCGACCG
GCCGGCCGCTCGTCCTCATCGACATCCGCGCGCCCGGCCTGCGCTCGATCAACGTCGACAACGCGGGCGGCGCGGCGCTC
GCGATGCATCACCTGTTCGCGTCGGGCCGCCGGCGCGTCGCGTTCATCGGCGGCTCGCTCGCGCACTACAGCATCGCGCA
GCGCGCGCTCGGCTATCGCCGCGCCTTCTTCGACGCGGGCATGCTGTTCGATCCTTCGCTCGAGCTGTCGATCGACACGA
GCCTCGGCCCCGATGCCGGCGCGGCAAACGCGATGCGGCAGTTGCTCGATGCGGGCGGCGCGCTGCCCGACGCGGTGTTC
GCGTACAACGACGCGGCCGCGCTCGCCGCGATGCGCGTGTGCGTCGAGCGCGGCGTGCGCGTGCCCGAGGACATCGCATT
CGTCGGCTTCGACGACATCCCGGCCGCCGCGCACGCGGCCCCACCCCTCACGACGCTCGCGATCGACAAGGAAGCGCTCG
GCCGGCAAGGCGTCGAGCTGCTGCTCGCCGATTCGCCCGCGCAAGCCGAACTCAGCCTCCCCGTGCAGCTCGTCGCGCGG
GCCAGCAGCGCAGGCGCCGCACGCGCGCCGCGCACCCTCACCTCGGTACTCGGATCATGA

Upstream 100 bases:

>100_bases
ATAAATAAGCGCTTATAAGCAGCACCCGCCCCACCCCGGAACGTTTCGCGGCGTTTTTTACTATACAATCGCCAACACCC
TAAACATCCCGGGTCAAACG

Downstream 100 bases:

>100_bases
CGACCTCAGCCTCGCCCGCCCCCCGTCCGGCGCCCGCCGCGCCGCCCGCCGCCGCGCCGTACGTCGCGAGCTTTCGCGAC
CCGCAGTTCCTGCTCGCGCA

Product: LacI family regulatory protein

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor [H]

Number of amino acids: Translated: 339; Mature: 338

Protein sequence:

>339_residues
MGTTIRDVAQAANVSIGTVSRALKNQPGLSEATRARIVEIAHRMNYDPTQLRPRIKRLTFLLHRQHNNFATTPFFSHVLH
GVEDACRERGIVPSLLTTGPTDDVIRQMRPHAPDAIAVAGFMEPETLEALAATGRPLVLIDIRAPGLRSINVDNAGGAAL
AMHHLFASGRRRVAFIGGSLAHYSIAQRALGYRRAFFDAGMLFDPSLELSIDTSLGPDAGAANAMRQLLDAGGALPDAVF
AYNDAAALAAMRVCVERGVRVPEDIAFVGFDDIPAAAHAAPPLTTLAIDKEALGRQGVELLLADSPAQAELSLPVQLVAR
ASSAGAARAPRTLTSVLGS

Sequences:

>Translated_339_residues
MGTTIRDVAQAANVSIGTVSRALKNQPGLSEATRARIVEIAHRMNYDPTQLRPRIKRLTFLLHRQHNNFATTPFFSHVLH
GVEDACRERGIVPSLLTTGPTDDVIRQMRPHAPDAIAVAGFMEPETLEALAATGRPLVLIDIRAPGLRSINVDNAGGAAL
AMHHLFASGRRRVAFIGGSLAHYSIAQRALGYRRAFFDAGMLFDPSLELSIDTSLGPDAGAANAMRQLLDAGGALPDAVF
AYNDAAALAAMRVCVERGVRVPEDIAFVGFDDIPAAAHAAPPLTTLAIDKEALGRQGVELLLADSPAQAELSLPVQLVAR
ASSAGAARAPRTLTSVLGS
>Mature_338_residues
GTTIRDVAQAANVSIGTVSRALKNQPGLSEATRARIVEIAHRMNYDPTQLRPRIKRLTFLLHRQHNNFATTPFFSHVLHG
VEDACRERGIVPSLLTTGPTDDVIRQMRPHAPDAIAVAGFMEPETLEALAATGRPLVLIDIRAPGLRSINVDNAGGAALA
MHHLFASGRRRVAFIGGSLAHYSIAQRALGYRRAFFDAGMLFDPSLELSIDTSLGPDAGAANAMRQLLDAGGALPDAVFA
YNDAAALAAMRVCVERGVRVPEDIAFVGFDDIPAAAHAAPPLTTLAIDKEALGRQGVELLLADSPAQAELSLPVQLVARA
SSAGAARAPRTLTSVLGS

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790369, Length=324, Percent_Identity=31.7901234567901, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1790194, Length=313, Percent_Identity=31.629392971246, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI1789456, Length=315, Percent_Identity=27.6190476190476, Blast_Score=106, Evalue=3e-24,
Organism=Escherichia coli, GI1789202, Length=293, Percent_Identity=30.7167235494881, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1787948, Length=315, Percent_Identity=27.9365079365079, Blast_Score=98, Evalue=8e-22,
Organism=Escherichia coli, GI1786540, Length=365, Percent_Identity=27.9452054794521, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1788474, Length=329, Percent_Identity=27.0516717325228, Blast_Score=91, Evalue=8e-20,
Organism=Escherichia coli, GI1789068, Length=310, Percent_Identity=27.0967741935484, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1787580, Length=315, Percent_Identity=26.031746031746, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI48994940, Length=315, Percent_Identity=28.2539682539683, Blast_Score=81, Evalue=8e-17,
Organism=Escherichia coli, GI1787906, Length=307, Percent_Identity=26.7100977198697, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI1790715, Length=317, Percent_Identity=25.2365930599369, Blast_Score=66, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 35895; Mature: 35763

Theoretical pI: Translated: 7.79; Mature: 7.79

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGTTIRDVAQAANVSIGTVSRALKNQPGLSEATRARIVEIAHRMNYDPTQLRPRIKRLTF
CCCHHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
LLHRQHNNFATTPFFSHVLHGVEDACRERGIVPSLLTTGPTDDVIRQMRPHAPDAIAVAG
HHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHCCCCCHHHHHHHCCCCCCCEEEEC
FMEPETLEALAATGRPLVLIDIRAPGLRSINVDNAGGAALAMHHLFASGRRRVAFIGGSL
CCCHHHHHHHHHCCCCEEEEEECCCCCCEECCCCCCCHHHHHHHHHHCCCCEEEEECCCH
AHYSIAQRALGYRRAFFDAGMLFDPSLELSIDTSLGPDAGAANAMRQLLDAGGALPDAVF
HHHHHHHHHHHHHHHHHHCCCEECCCCEEEEECCCCCCCCHHHHHHHHHHCCCCCCCHHH
AYNDAAALAAMRVCVERGVRVPEDIAFVGFDDIPAAAHAAPPLTTLAIDKEALGRQGVEL
HCCHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCHHHCCCCCEEEEECHHHHCCCCCEE
LLADSPAQAELSLPVQLVARASSAGAARAPRTLTSVLGS
EEECCCCCCCCCCCHHHHHHCCCCCCCCCHHHHHHHHCC
>Mature Secondary Structure 
GTTIRDVAQAANVSIGTVSRALKNQPGLSEATRARIVEIAHRMNYDPTQLRPRIKRLTF
CCHHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
LLHRQHNNFATTPFFSHVLHGVEDACRERGIVPSLLTTGPTDDVIRQMRPHAPDAIAVAG
HHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHCCCCCHHHHHHHCCCCCCCEEEEC
FMEPETLEALAATGRPLVLIDIRAPGLRSINVDNAGGAALAMHHLFASGRRRVAFIGGSL
CCCHHHHHHHHHCCCCEEEEEECCCCCCEECCCCCCCHHHHHHHHHHCCCCEEEEECCCH
AHYSIAQRALGYRRAFFDAGMLFDPSLELSIDTSLGPDAGAANAMRQLLDAGGALPDAVF
HHHHHHHHHHHHHHHHHHCCCEECCCCEEEEECCCCCCCCHHHHHHHHHHCCCCCCCHHH
AYNDAAALAAMRVCVERGVRVPEDIAFVGFDDIPAAAHAAPPLTTLAIDKEALGRQGVEL
HCCHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCHHHCCCCCEEEEECHHHHCCCCCEE
LLADSPAQAELSLPVQLVARASSAGAARAPRTLTSVLGS
EEECCCCCCCCCCCHHHHHHCCCCCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA