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The map label for this gene is ttcA

Identifier: 53717955

GI number: 53717955

Start: 335475

End: 336470

Strand: Reverse

Name: ttcA

Synonym: BPSL0314

Alternate gene names: 53717955

Gene position: 336470-335475 (Counterclockwise)

Preceding gene: 53717956

Following gene: 53717954

Centisome position: 8.26

GC content: 65.76

Gene sequence:

>996_bases
ATGAATGCGCCCCATACCCCGCATCTGAACGAAGCCGAAGCGGCCGCCGCCGTCGAGGCGAACGCGGCCGAGCTCGGCCG
CCGCGCGCTCACGCGCCGCGAGCAGAAGGAAGCGTACGAGAACAACAAGCTGTTCAAGCGGCTCGTGCGCCAGGTCGGCC
AGGCGATCGGCGATTACAACATGATCGAGCACGGCGACAAGGTGATGGTCTGCCTGTCGGGCGGCAAGGACAGCTACGCG
CTGCTCGACATCCTGCTGCGGCTGCGCGAGCGCGCGCCGATCGATTTCGACATCGTCGCCGTGAACCTCGACCAGAAGCA
GCCGGGCTTCCCGGAGCACGTGCTGCCCGAGTACCTGACGAAGATCGGCGTGCCGTTCCACATCGAGAATCAGGATACGT
ACAGCATCGTCAAGCGCCTCGTGCCCGAAGGCAAGACCACCTGCTCGCTGTGCTCGCGGCTGCGCCGCGGGATCCTGTAC
CGCGTCGCGGGCGAGCTCGGCGCGACGAAGATCGCGCTCGGCCACCACCGCGACGACATCGTGCAGACGCTGCTGCTGAA
CATGTTCTACGGCGGCAAGCTGAAGGGGATGCCGCCGAAGCTGCAATCGGACGACGGCAAGAACATCGTGATCCGTCCGC
TCGCGTACGCGAAGGAAACCGATCTCGAGAAATACGCGGAACTGCGCGAATTCCCGATCATTCCGTGCAACCTGTGCGGC
AGCCAGCCGAACCTGAAGCGCGCGGAAATGAAGGCGCTGATCCGCGACTGGGACAAGCGCTTCCCGGGCCGCGTCGACAA
CATGTTCAACGCGCTCGCGAACGTCGTGCCCTCGCACCTGATGGACGCGCGGCTCTTCCCGTTCGCCGGCCTGCGTGCGA
CGGGCGAAGCCGATCCGAACGGCGACATCGCGTTCGACGAGGACCCGTGCGGCACCGACGCGAGCGCGCCGGGCGGCGCG
AAATCCGTGTCGATCGTGCAGTTCGACGATCTGTAA

Upstream 100 bases:

>100_bases
CGGTGCGCGCGGTGCGCGTGTCCACCGAGAAGCCGGACGCCTACCCCGATTGCGACGCCGTCGGCGTCGAAGTATTTCGC
ATCAAGGACGAGGAGCGAGC

Downstream 100 bases:

>100_bases
TCCGTCAATCGCGGCGCGCGCCGCGAGCCGGAACGAATCCGCGGCGAACGGCGCGCGCGGCACGCCGCCCGCGCGCCACG
CCCGACGCACGCCGACGCCC

Product: C32 tRNA thiolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 331; Mature: 331

Protein sequence:

>331_residues
MNAPHTPHLNEAEAAAAVEANAAELGRRALTRREQKEAYENNKLFKRLVRQVGQAIGDYNMIEHGDKVMVCLSGGKDSYA
LLDILLRLRERAPIDFDIVAVNLDQKQPGFPEHVLPEYLTKIGVPFHIENQDTYSIVKRLVPEGKTTCSLCSRLRRGILY
RVAGELGATKIALGHHRDDIVQTLLLNMFYGGKLKGMPPKLQSDDGKNIVIRPLAYAKETDLEKYAELREFPIIPCNLCG
SQPNLKRAEMKALIRDWDKRFPGRVDNMFNALANVVPSHLMDARLFPFAGLRATGEADPNGDIAFDEDPCGTDASAPGGA
KSVSIVQFDDL

Sequences:

>Translated_331_residues
MNAPHTPHLNEAEAAAAVEANAAELGRRALTRREQKEAYENNKLFKRLVRQVGQAIGDYNMIEHGDKVMVCLSGGKDSYA
LLDILLRLRERAPIDFDIVAVNLDQKQPGFPEHVLPEYLTKIGVPFHIENQDTYSIVKRLVPEGKTTCSLCSRLRRGILY
RVAGELGATKIALGHHRDDIVQTLLLNMFYGGKLKGMPPKLQSDDGKNIVIRPLAYAKETDLEKYAELREFPIIPCNLCG
SQPNLKRAEMKALIRDWDKRFPGRVDNMFNALANVVPSHLMDARLFPFAGLRATGEADPNGDIAFDEDPCGTDASAPGGA
KSVSIVQFDDL
>Mature_331_residues
MNAPHTPHLNEAEAAAAVEANAAELGRRALTRREQKEAYENNKLFKRLVRQVGQAIGDYNMIEHGDKVMVCLSGGKDSYA
LLDILLRLRERAPIDFDIVAVNLDQKQPGFPEHVLPEYLTKIGVPFHIENQDTYSIVKRLVPEGKTTCSLCSRLRRGILY
RVAGELGATKIALGHHRDDIVQTLLLNMFYGGKLKGMPPKLQSDDGKNIVIRPLAYAKETDLEKYAELREFPIIPCNLCG
SQPNLKRAEMKALIRDWDKRFPGRVDNMFNALANVVPSHLMDARLFPFAGLRATGEADPNGDIAFDEDPCGTDASAPGGA
KSVSIVQFDDL

Specific function: Required for the thiolation of cytidine in position 32 of tRNA, to form 2-thiocytidine (s(2)C32)

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TtcA family

Homologues:

Organism=Escherichia coli, GI1787606, Length=274, Percent_Identity=68.2481751824817, Blast_Score=408, Evalue=1e-115,
Organism=Caenorhabditis elegans, GI193211090, Length=285, Percent_Identity=30.1754385964912, Blast_Score=127, Evalue=6e-30,
Organism=Caenorhabditis elegans, GI17540090, Length=237, Percent_Identity=29.1139240506329, Blast_Score=84, Evalue=8e-17,
Organism=Drosophila melanogaster, GI24651972, Length=246, Percent_Identity=27.2357723577236, Blast_Score=87, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TTCA_BURM7 (A3MND8)

Other databases:

- EMBL:   CP000548
- RefSeq:   YP_001081774.1
- ProteinModelPortal:   A3MND8
- SMR:   A3MND8
- GeneID:   4892199
- GenomeReviews:   CP000548_GR
- KEGG:   bmn:BMA10247_2245
- TIGR:   BMA10247_2245
- HOGENOM:   HBG347038
- OMA:   EHILPEY
- ProtClustDB:   PRK10696
- BioCyc:   BMAL320389:BMA10247_2245-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01850
- InterPro:   IPR012089
- InterPro:   IPR014729
- InterPro:   IPR011063
- Gene3D:   G3DSA:3.40.50.620
- PIRSF:   PIRSF004976

Pfam domain/function: PF01171 ATP_bind_3

EC number: NA

Molecular weight: Translated: 36720; Mature: 36720

Theoretical pI: Translated: 6.94; Mature: 6.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAPHTPHLNEAEAAAAVEANAAELGRRALTRREQKEAYENNKLFKRLVRQVGQAIGDYN
CCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
MIEHGDKVMVCLSGGKDSYALLDILLRLRERAPIDFDIVAVNLDQKQPGFPEHVLPEYLT
HHCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCHHHHHHHHH
KIGVPFHIENQDTYSIVKRLVPEGKTTCSLCSRLRRGILYRVAGELGATKIALGHHRDDI
HCCCCEEECCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHH
VQTLLLNMFYGGKLKGMPPKLQSDDGKNIVIRPLAYAKETDLEKYAELREFPIIPCNLCG
HHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEECCHHHHHCCHHHHHHHHHCCCCCHHCCC
SQPNLKRAEMKALIRDWDKRFPGRVDNMFNALANVVPSHLMDARLFPFAGLRATGEADPN
CCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
GDIAFDEDPCGTDASAPGGAKSVSIVQFDDL
CCEEECCCCCCCCCCCCCCCCEEEEEEECCC
>Mature Secondary Structure
MNAPHTPHLNEAEAAAAVEANAAELGRRALTRREQKEAYENNKLFKRLVRQVGQAIGDYN
CCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
MIEHGDKVMVCLSGGKDSYALLDILLRLRERAPIDFDIVAVNLDQKQPGFPEHVLPEYLT
HHCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCHHHHHHHHH
KIGVPFHIENQDTYSIVKRLVPEGKTTCSLCSRLRRGILYRVAGELGATKIALGHHRDDI
HCCCCEEECCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHH
VQTLLLNMFYGGKLKGMPPKLQSDDGKNIVIRPLAYAKETDLEKYAELREFPIIPCNLCG
HHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEECCHHHHHCCHHHHHHHHHCCCCCHHCCC
SQPNLKRAEMKALIRDWDKRFPGRVDNMFNALANVVPSHLMDARLFPFAGLRATGEADPN
CCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
GDIAFDEDPCGTDASAPGGAKSVSIVQFDDL
CCEEECCCCCCCCCCCCCCCCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA