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The map label for this gene is pnp

Identifier: 52842967

GI number: 52842967

Start: 3117904

End: 3120093

Strand: Reverse

Name: pnp

Synonym: lpg2768

Alternate gene names: 52842967

Gene position: 3120093-3117904 (Counterclockwise)

Preceding gene: 52842968

Following gene: 52842966

Centisome position: 91.83

GC content: 41.55

Gene sequence:

>2190_bases
GTGGCAAAAATTACAAAAGAAATAGTATTCGGTAACCATAAATTAATCTTGGAAACAGGTGAAGTAGCACGACAGGCTGA
TGGTGCCGTAATGGCAAGTATGAATGGTACACAAGTGCTAGTGACTGTAGTTTGGAAAAAGGATGGTGGTGAAAGTAATG
ATTTTTTCCCATTAACAGTGAATTATCAAGAAAAGTTCTATGCCATAGGTAAAATCCCTGGTGGCTTTAACAAACGTGAA
GGACGGCCATCTGATAATGAAACATTAATTTCTCGGTTAATAGATAGACCGATCCGGCCATTATTCCCGGATAATTTTTT
TAACGAAGTACAGATAATTGCTACTGTACTGTCCTTAAATCCCGAGGTATCTCCTGATATTATCGCTATGATTGGAGCCT
CCGCAGCGCTTTCAATTTCTGGCGTGCCATTTAATGGCCCTATAGGTGCCGCTCGAGTAGGCTATAAAGATGGCGTTTAT
CTGCTCAATCCAAGTAGAAAAGAACAAGAAGAGTCTAAGCTTGATTTGGTTATTGCGGGAACAAAAGATGCCATTTTGAT
GGTGGAATCAGAAGCGCAGGAATTAAGTGAAGACATCATGCGTGGTGCTATGTTATATGGCCATGAAATGATGAAAAATG
TCATAAAATCAATAGAAGAACTTGCTAGAGACGTGGGTAAGAGTAAACCTGAGTGGAAAGCACCAGAAATTGATACAGTA
CTAAAAGCTAGAATCAATGACGTAGCTAGGAATGAAGTTGAAGCCGCTTATCTTATTAAGGACAAGCAACAACGGTATCA
GCGATTAGACGAGTTAAGAGAGCAAACTATTTCTGCTTTACTGGCTGAAAATGATGAGTTAAATGCAGATGTTATTGCCA
ATATGTTTGGTGAGCTAGAACGTTCTATAGTACGTAATCGTATTCTTGACGGCGAGCCTCGCATCGATGGTCGTGATCAC
AGAACAGTTAGGCCTATTTCTATTCGTACCAAATTCTTGGAAAGAACTCATGGTTCCTGTTTGTTTACCAGAGGAGAAAC
TCAAGCTATTGTTGTTGCCACTTTGGGTAATGAACGTGATGCACAGATATTAGACGGAATTAGTGGCGAAAGCAGAGATC
GATTTATGCTTCATTATAATTTCCCTCCTTATTCTGTCGGCGAAACAGGGCAAGTGGGCAGTCCTAAACGGCGTGAAATT
GGGCATGGCCGTTTAGCCAAGCGTGCTTTAATGGCTGTACTGCCTGATGCTAATGAGTTTCCTTATGTGCTACGTATTGT
ATCTGAGATTACGGAATCCAATGGCTCCAGTTCTATGGCTACTGTTTGTGGAACAAGTCTGGCATTGATGGATGCCGGGG
TACCTTTAAAAGCACCAGTCGCTGGTGTGGCCATGGGCTTAATCAAGGAAGGTGATCGTTATGCTGTATTAACAGACATA
TTGGGTGATGAGGATCATTTAGGTGATATGGACTTTAAAGTGGCCGGTACGGAAAAAGGGATTACTGCTTTGCAGATGGA
TATCAAAATTTCCGGGATTACCAATGAAATTATGGAACAGGCTTTGGAGCAAGCATTGGAAGGTCGTACCCATATTCTTG
GTGTCATGAATAATGCTCTTGCCGAGCATAGAACCGAGTTATCTCAGCACGCTCCAAGAATCACAACCATGAAAGTCGCT
GAAGATAAAATCCGCACAATTATTGGCAAAGGTGGGGCAACGATTAAAGGGCTTATTGAAAGTACCGGCGTATCTATAGA
TATTGATGACTCAGGGGTTGTCCAATTATTTTCTCCGGATAAGATGGCTTTGGAAGAGGCGCAAAAACAAATTAAAGCTT
TAATTGCTGAAATTGAAGTGGGTCAGACTTATCAAGGGAAAGTAAGCAAGATAGTTGACTTTGGAGCTTTTATCAATTTA
CTGCCTGGTAAAGATGGCTTACTGCATATTTCACAAATATGTGCCGACAGAACACAAAAGGTAGAAGAGGTATTGCAGGA
AGGTCAGGAAATTGAGGTCTTTGTTGCTGGGATAGATAAGCAAGGGCGAGTCAAACTGGAGTGGAAGGATAAGCCGCAAG
CTGAAGCCAAAGAGGTCGAAGACGCCCCGGTATCTGCTACGTTTCTTACAATGGAAGAGCAATCTGAAGAAATTAATTCA
GGCAACAAAATCTCTGAAGAAGAGGAATAA

Upstream 100 bases:

>100_bases
GTCTCTATGGGGACGCATCAAATAATAAATCAGTTTTAGAATAAAAGGCGCAGCGTTCTGCGCCTTTTTTTTCTTATATT
TACTTAATGGGGAACATTAC

Downstream 100 bases:

>100_bases
TAAGATAGTAGCATTTCAAGTTCTATTTATTATAAACTCGTGGAAGGCATGAGCTTTTCGCGAGTTTTAACTGAGTGCTT
GCAATTGGTATATATGACCG

Product: polynucleotide phosphorylase/polyadenylase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase [H]

Number of amino acids: Translated: 729; Mature: 728

Protein sequence:

>729_residues
MAKITKEIVFGNHKLILETGEVARQADGAVMASMNGTQVLVTVVWKKDGGESNDFFPLTVNYQEKFYAIGKIPGGFNKRE
GRPSDNETLISRLIDRPIRPLFPDNFFNEVQIIATVLSLNPEVSPDIIAMIGASAALSISGVPFNGPIGAARVGYKDGVY
LLNPSRKEQEESKLDLVIAGTKDAILMVESEAQELSEDIMRGAMLYGHEMMKNVIKSIEELARDVGKSKPEWKAPEIDTV
LKARINDVARNEVEAAYLIKDKQQRYQRLDELREQTISALLAENDELNADVIANMFGELERSIVRNRILDGEPRIDGRDH
RTVRPISIRTKFLERTHGSCLFTRGETQAIVVATLGNERDAQILDGISGESRDRFMLHYNFPPYSVGETGQVGSPKRREI
GHGRLAKRALMAVLPDANEFPYVLRIVSEITESNGSSSMATVCGTSLALMDAGVPLKAPVAGVAMGLIKEGDRYAVLTDI
LGDEDHLGDMDFKVAGTEKGITALQMDIKISGITNEIMEQALEQALEGRTHILGVMNNALAEHRTELSQHAPRITTMKVA
EDKIRTIIGKGGATIKGLIESTGVSIDIDDSGVVQLFSPDKMALEEAQKQIKALIAEIEVGQTYQGKVSKIVDFGAFINL
LPGKDGLLHISQICADRTQKVEEVLQEGQEIEVFVAGIDKQGRVKLEWKDKPQAEAKEVEDAPVSATFLTMEEQSEEINS
GNKISEEEE

Sequences:

>Translated_729_residues
MAKITKEIVFGNHKLILETGEVARQADGAVMASMNGTQVLVTVVWKKDGGESNDFFPLTVNYQEKFYAIGKIPGGFNKRE
GRPSDNETLISRLIDRPIRPLFPDNFFNEVQIIATVLSLNPEVSPDIIAMIGASAALSISGVPFNGPIGAARVGYKDGVY
LLNPSRKEQEESKLDLVIAGTKDAILMVESEAQELSEDIMRGAMLYGHEMMKNVIKSIEELARDVGKSKPEWKAPEIDTV
LKARINDVARNEVEAAYLIKDKQQRYQRLDELREQTISALLAENDELNADVIANMFGELERSIVRNRILDGEPRIDGRDH
RTVRPISIRTKFLERTHGSCLFTRGETQAIVVATLGNERDAQILDGISGESRDRFMLHYNFPPYSVGETGQVGSPKRREI
GHGRLAKRALMAVLPDANEFPYVLRIVSEITESNGSSSMATVCGTSLALMDAGVPLKAPVAGVAMGLIKEGDRYAVLTDI
LGDEDHLGDMDFKVAGTEKGITALQMDIKISGITNEIMEQALEQALEGRTHILGVMNNALAEHRTELSQHAPRITTMKVA
EDKIRTIIGKGGATIKGLIESTGVSIDIDDSGVVQLFSPDKMALEEAQKQIKALIAEIEVGQTYQGKVSKIVDFGAFINL
LPGKDGLLHISQICADRTQKVEEVLQEGQEIEVFVAGIDKQGRVKLEWKDKPQAEAKEVEDAPVSATFLTMEEQSEEINS
GNKISEEEE
>Mature_728_residues
AKITKEIVFGNHKLILETGEVARQADGAVMASMNGTQVLVTVVWKKDGGESNDFFPLTVNYQEKFYAIGKIPGGFNKREG
RPSDNETLISRLIDRPIRPLFPDNFFNEVQIIATVLSLNPEVSPDIIAMIGASAALSISGVPFNGPIGAARVGYKDGVYL
LNPSRKEQEESKLDLVIAGTKDAILMVESEAQELSEDIMRGAMLYGHEMMKNVIKSIEELARDVGKSKPEWKAPEIDTVL
KARINDVARNEVEAAYLIKDKQQRYQRLDELREQTISALLAENDELNADVIANMFGELERSIVRNRILDGEPRIDGRDHR
TVRPISIRTKFLERTHGSCLFTRGETQAIVVATLGNERDAQILDGISGESRDRFMLHYNFPPYSVGETGQVGSPKRREIG
HGRLAKRALMAVLPDANEFPYVLRIVSEITESNGSSSMATVCGTSLALMDAGVPLKAPVAGVAMGLIKEGDRYAVLTDIL
GDEDHLGDMDFKVAGTEKGITALQMDIKISGITNEIMEQALEQALEGRTHILGVMNNALAEHRTELSQHAPRITTMKVAE
DKIRTIIGKGGATIKGLIESTGVSIDIDDSGVVQLFSPDKMALEEAQKQIKALIAEIEVGQTYQGKVSKIVDFGAFINLL
PGKDGLLHISQICADRTQKVEEVLQEGQEIEVFVAGIDKQGRVKLEWKDKPQAEAKEVEDAPVSATFLTMEEQSEEINSG
NKISEEEE

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction [H]

COG id: COG1185

COG function: function code J; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Homo sapiens, GI188528628, Length=747, Percent_Identity=36.813922356091, Blast_Score=448, Evalue=1e-126,
Organism=Escherichia coli, GI145693187, Length=687, Percent_Identity=59.6797671033479, Blast_Score=855, Evalue=0.0,
Organism=Caenorhabditis elegans, GI115534063, Length=660, Percent_Identity=34.8484848484849, Blast_Score=347, Evalue=2e-95,
Organism=Drosophila melanogaster, GI281362905, Length=710, Percent_Identity=37.6056338028169, Blast_Score=456, Evalue=1e-128,
Organism=Drosophila melanogaster, GI24651641, Length=710, Percent_Identity=37.6056338028169, Blast_Score=456, Evalue=1e-128,
Organism=Drosophila melanogaster, GI24651643, Length=710, Percent_Identity=37.6056338028169, Blast_Score=456, Evalue=1e-128,
Organism=Drosophila melanogaster, GI161079377, Length=643, Percent_Identity=37.4805598755832, Blast_Score=422, Evalue=1e-118,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967 [H]

Pfam domain/function: PF00013 KH_1; PF03726 PNPase; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1 [H]

EC number: =2.7.7.8 [H]

Molecular weight: Translated: 80108; Mature: 79977

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: PS50084 KH_TYPE_1 ; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKITKEIVFGNHKLILETGEVARQADGAVMASMNGTQVLVTVVWKKDGGESNDFFPLTV
CCCCCHHHHCCCCEEEEECCHHHHHCCCCEEEECCCCEEEEEEEEECCCCCCCCEEEEEE
NYQEKFYAIGKIPGGFNKREGRPSDNETLISRLIDRPIRPLFPDNFFNEVQIIATVLSLN
ECCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCC
PEVSPDIIAMIGASAALSISGVPFNGPIGAARVGYKDGVYLLNPSRKEQEESKLDLVIAG
CCCCCHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCCCEEEECCCCCCCHHCCEEEEEEC
TKDAILMVESEAQELSEDIMRGAMLYGHEMMKNVIKSIEELARDVGKSKPEWKAPEIDTV
CCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHH
LKARINDVARNEVEAAYLIKDKQQRYQRLDELREQTISALLAENDELNADVIANMFGELE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
RSIVRNRILDGEPRIDGRDHRTVRPISIRTKFLERTHGSCLFTRGETQAIVVATLGNERD
HHHHHHHCCCCCCCCCCCCCCEECCHHHHHHHHHHCCCCEEEECCCCCEEEEEECCCCCC
AQILDGISGESRDRFMLHYNFPPYSVGETGQVGSPKRREIGHGRLAKRALMAVLPDANEF
HHHHCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCHHHHCCCHHHHHHHHHHHCCCCCCC
PYVLRIVSEITESNGSSSMATVCGTSLALMDAGVPLKAPVAGVAMGLIKEGDRYAVLTDI
HHHHHHHHHHHHCCCCCCHHHHHCCHHHHHCCCCCCCCCHHHHHHHHHHCCCCEEEEEEC
LGDEDHLGDMDFKVAGTEKGITALQMDIKISGITNEIMEQALEQALEGRTHILGVMNNAL
CCCCCCCCCCCEEEECCCCCCEEEEEEEEEECCHHHHHHHHHHHHHCCCHHHHHHHHHHH
AEHRTELSQHAPRITTMKVAEDKIRTIIGKGGATIKGLIESTGVSIDIDDSGVVQLFSPD
HHHHHHHHHHCCCEEEEHHHHHHHHHHHCCCCCHHHHHHHHCCCEEEECCCCEEEEECCC
KMALEEAQKQIKALIAEIEVGQTYQGKVSKIVDFGAFINLLPGKDGLLHISQICADRTQK
HHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHH
VEEVLQEGQEIEVFVAGIDKQGRVKLEWKDKPQAEAKEVEDAPVSATFLTMEEQSEEINS
HHHHHHCCCEEEEEEEECCCCCEEEEEECCCCCCHHHHCCCCCCCEEEEEEHHHHHHHCC
GNKISEEEE
CCCCCCCCC
>Mature Secondary Structure 
AKITKEIVFGNHKLILETGEVARQADGAVMASMNGTQVLVTVVWKKDGGESNDFFPLTV
CCCCHHHHCCCCEEEEECCHHHHHCCCCEEEECCCCEEEEEEEEECCCCCCCCEEEEEE
NYQEKFYAIGKIPGGFNKREGRPSDNETLISRLIDRPIRPLFPDNFFNEVQIIATVLSLN
ECCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCC
PEVSPDIIAMIGASAALSISGVPFNGPIGAARVGYKDGVYLLNPSRKEQEESKLDLVIAG
CCCCCHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCCCEEEECCCCCCCHHCCEEEEEEC
TKDAILMVESEAQELSEDIMRGAMLYGHEMMKNVIKSIEELARDVGKSKPEWKAPEIDTV
CCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHH
LKARINDVARNEVEAAYLIKDKQQRYQRLDELREQTISALLAENDELNADVIANMFGELE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
RSIVRNRILDGEPRIDGRDHRTVRPISIRTKFLERTHGSCLFTRGETQAIVVATLGNERD
HHHHHHHCCCCCCCCCCCCCCEECCHHHHHHHHHHCCCCEEEECCCCCEEEEEECCCCCC
AQILDGISGESRDRFMLHYNFPPYSVGETGQVGSPKRREIGHGRLAKRALMAVLPDANEF
HHHHCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCHHHHCCCHHHHHHHHHHHCCCCCCC
PYVLRIVSEITESNGSSSMATVCGTSLALMDAGVPLKAPVAGVAMGLIKEGDRYAVLTDI
HHHHHHHHHHHHCCCCCCHHHHHCCHHHHHCCCCCCCCCHHHHHHHHHHCCCCEEEEEEC
LGDEDHLGDMDFKVAGTEKGITALQMDIKISGITNEIMEQALEQALEGRTHILGVMNNAL
CCCCCCCCCCCEEEECCCCCCEEEEEEEEEECCHHHHHHHHHHHHHCCCHHHHHHHHHHH
AEHRTELSQHAPRITTMKVAEDKIRTIIGKGGATIKGLIESTGVSIDIDDSGVVQLFSPD
HHHHHHHHHHCCCEEEEHHHHHHHHHHHCCCCCHHHHHHHHCCCEEEECCCCEEEEECCC
KMALEEAQKQIKALIAEIEVGQTYQGKVSKIVDFGAFINLLPGKDGLLHISQICADRTQK
HHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHH
VEEVLQEGQEIEVFVAGIDKQGRVKLEWKDKPQAEAKEVEDAPVSATFLTMEEQSEEINS
HHHHHHCCCEEEEEEEECCCCCEEEEEECCCCCCHHHHCCCCCCCEEEEEEHHHHHHHCC
GNKISEEEE
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA