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The map label for this gene is rne

Identifier: 52842507

GI number: 52842507

Start: 2594148

End: 2596151

Strand: Direct

Name: rne

Synonym: lpg2297

Alternate gene names: 52842507

Gene position: 2594148-2596151 (Clockwise)

Preceding gene: 52842506

Following gene: 52842509

Centisome position: 76.35

GC content: 41.17

Gene sequence:

>2004_bases
ATGGGGTTATACATGGAAAAAATGCTAATCAATGCAATGCAAACAGAAGAAATTCGTGTTGCACTAATTAAAAACAATCA
ATTATTTGATCTGGACATTGAATGCCCGGGAGAAATAAAGAAAAAAGGTAATATTTATAAAGCCATTGTAACAAGAAGAG
AACCCAGCCTTGATGCTGTTTTCGTTGAATATGGTTCCAAACGCCAAGGTTTCTTACCCCTAAAAGAAATAGCGCCTGAA
TACTTAAGCAAGAATCCGGAAGAGTTTGGAGATGAGAAACCTCCAATTACCAGCCTCATCCGTGAAGGTCAAGAATTACT
TATCCAAGTGGATAAAGAAGAAAGAGGGAATAAAGGAGCAGCACTGACAACTTTTATTACCCTGGCAGGTTGCTACCTGG
TTTTAATGCCCAATAACCCTCATTCGGGCGGAATATCAAGACGTATTGAAGGTGATGAGCGTGATGAATTACGCGAAACA
TTAAATGCTTTAACACTCCCTGAAGACATGGGCCTTATTATTCGCACTGCAGGTGTTGGAAAAAGTCTGGAAGAACTGCA
AGATGACTTAAACATGCTTTGCAATCAGTGGCAATCCATCAAGGAAGCTTATAATTCAGAACTGGCACCTTGCCTGATTC
ATCAAGAGGGCGATGTCATTATTCGTTCCATTCGTGACAATTTGCGAAAATCCATCAGTGAAATTATTATTGATGATCAA
ATATCCTATATCAAAGCAAAACAATACATTGAACGCGTAAAACCAGAATTTTTACCTAATCTAAAACTCTATAACAGCAG
CATCCCTCTTTTTAATTTCTATCAGATTGAAAGCCAAATTGAGACAGCTTATCAGCGCCAGGTCATGCTTCCTTCTGGAG
GTGCCTTGGTCATAGATAGAACTGAAGCTCTGGTATCAATTGATATTAACTCAGCCAAGGCTACAGGCGGTGCCGACATT
GAAGCGACTGCATTAAATACCAACCTTGAAGCAGCTGATGAAATTGCTCGCCAGCTTCGCCTGCGTGATTTAGGTGGTTT
GGTAGTTATTGATTTTATTGACATGAGTTCTAGTAAAAATCAAAGAGAGGTAGAAAATCGACTCAAAGAAGCACTGCAGG
CTGATAGGGCCAGAATTCAAGTAGGGCGAATTTCACGCTTTGGCCTTCTTGAAATGTCAAGGCAACGCCTCCGATTATCA
TTAGGAGAAACTGCCCAGGAAATTTGTCCACGTTGTGAAGGTAGAGGAACTGTACGCAGTATACAGTCTCATGGCTTATC
AATTACCCGCCTTATCGAAGAAGAGGCGCTCAAGGAAAAAACAGCTGAAATTCAAGTGCAATTGCCACCAGAAATGGCTA
CATTCATTATGAATGAAAAACGTAATTTTATCAGGGACATTGAAAAAAGGCATGGTGTCAGTGTTGTTGTTATTGCGAAT
CCTTATATGCACCCTCCACAATATACGATTACTCGCCTGAAAGAAGACAACGTAGGCAAATCTAAAAAACCAAGTTACTC
TTTGATTCAGCAACCTGAAGTACATGTTCCCAGCAGTGATAAAGAACCCGTGTCACATGATGAACCTGCTGTGAAATCAT
TTGCCGAACACAGGGTCATCAAACACCCTCAAACCAGTTTTATTAAACGATTATGGACTAGTTTATTTGGTGGTCATTCA
GTTGCCCACACCGTAACTCAAGAACCCGGTAAAAAACATCATCGTGCTCAAAATCAAGGTAAACACGCATCGTCCAAAAG
CTCAGGAGGAGACAAGCGTCAACAAGGCAGCAACGCAAATCGTAGACGTCGTTCAGGAGGAGGCCAGCAGCGTTCTTCAT
CAGGAAATACAAATGCTGCGGCCAGCACACATCAACAACGAAAAAAATCTAACGCAAATCAGCAAAGTCATGGCAGCCAA
CAAAGCAATCGTTCAATTCCTGCAAAAAATGAAGGCAACAAAAAAAGGGAAGCCATACTGGCTAAAGAGCCAGAGGAGAA
ATAG

Upstream 100 bases:

>100_bases
GGACCCGGAAAATTAACTTCATGGCAAGAAAACTGTAGAAATGATCATACGGCTATACTGAGCCTTACACTATCTCGATG
TAAATGCATGCGCCCTAAAT

Downstream 100 bases:

>100_bases
GCCGCCATATCAATTATTTGCCTATTCACCTTTTCAATAAGCACTTATCCGCACAGTCCAGTTAACTCTGGATTGTGCGG
GTAAAACACAGCACTCTTTC

Product: ribonuclease E

Products: NA

Alternate protein names: RNase E [H]

Number of amino acids: Translated: 667; Mature: 666

Protein sequence:

>667_residues
MGLYMEKMLINAMQTEEIRVALIKNNQLFDLDIECPGEIKKKGNIYKAIVTRREPSLDAVFVEYGSKRQGFLPLKEIAPE
YLSKNPEEFGDEKPPITSLIREGQELLIQVDKEERGNKGAALTTFITLAGCYLVLMPNNPHSGGISRRIEGDERDELRET
LNALTLPEDMGLIIRTAGVGKSLEELQDDLNMLCNQWQSIKEAYNSELAPCLIHQEGDVIIRSIRDNLRKSISEIIIDDQ
ISYIKAKQYIERVKPEFLPNLKLYNSSIPLFNFYQIESQIETAYQRQVMLPSGGALVIDRTEALVSIDINSAKATGGADI
EATALNTNLEAADEIARQLRLRDLGGLVVIDFIDMSSSKNQREVENRLKEALQADRARIQVGRISRFGLLEMSRQRLRLS
LGETAQEICPRCEGRGTVRSIQSHGLSITRLIEEEALKEKTAEIQVQLPPEMATFIMNEKRNFIRDIEKRHGVSVVVIAN
PYMHPPQYTITRLKEDNVGKSKKPSYSLIQQPEVHVPSSDKEPVSHDEPAVKSFAEHRVIKHPQTSFIKRLWTSLFGGHS
VAHTVTQEPGKKHHRAQNQGKHASSKSSGGDKRQQGSNANRRRRSGGGQQRSSSGNTNAAASTHQQRKKSNANQQSHGSQ
QSNRSIPAKNEGNKKREAILAKEPEEK

Sequences:

>Translated_667_residues
MGLYMEKMLINAMQTEEIRVALIKNNQLFDLDIECPGEIKKKGNIYKAIVTRREPSLDAVFVEYGSKRQGFLPLKEIAPE
YLSKNPEEFGDEKPPITSLIREGQELLIQVDKEERGNKGAALTTFITLAGCYLVLMPNNPHSGGISRRIEGDERDELRET
LNALTLPEDMGLIIRTAGVGKSLEELQDDLNMLCNQWQSIKEAYNSELAPCLIHQEGDVIIRSIRDNLRKSISEIIIDDQ
ISYIKAKQYIERVKPEFLPNLKLYNSSIPLFNFYQIESQIETAYQRQVMLPSGGALVIDRTEALVSIDINSAKATGGADI
EATALNTNLEAADEIARQLRLRDLGGLVVIDFIDMSSSKNQREVENRLKEALQADRARIQVGRISRFGLLEMSRQRLRLS
LGETAQEICPRCEGRGTVRSIQSHGLSITRLIEEEALKEKTAEIQVQLPPEMATFIMNEKRNFIRDIEKRHGVSVVVIAN
PYMHPPQYTITRLKEDNVGKSKKPSYSLIQQPEVHVPSSDKEPVSHDEPAVKSFAEHRVIKHPQTSFIKRLWTSLFGGHS
VAHTVTQEPGKKHHRAQNQGKHASSKSSGGDKRQQGSNANRRRRSGGGQQRSSSGNTNAAASTHQQRKKSNANQQSHGSQ
QSNRSIPAKNEGNKKREAILAKEPEEK
>Mature_666_residues
GLYMEKMLINAMQTEEIRVALIKNNQLFDLDIECPGEIKKKGNIYKAIVTRREPSLDAVFVEYGSKRQGFLPLKEIAPEY
LSKNPEEFGDEKPPITSLIREGQELLIQVDKEERGNKGAALTTFITLAGCYLVLMPNNPHSGGISRRIEGDERDELRETL
NALTLPEDMGLIIRTAGVGKSLEELQDDLNMLCNQWQSIKEAYNSELAPCLIHQEGDVIIRSIRDNLRKSISEIIIDDQI
SYIKAKQYIERVKPEFLPNLKLYNSSIPLFNFYQIESQIETAYQRQVMLPSGGALVIDRTEALVSIDINSAKATGGADIE
ATALNTNLEAADEIARQLRLRDLGGLVVIDFIDMSSSKNQREVENRLKEALQADRARIQVGRISRFGLLEMSRQRLRLSL
GETAQEICPRCEGRGTVRSIQSHGLSITRLIEEEALKEKTAEIQVQLPPEMATFIMNEKRNFIRDIEKRHGVSVVVIANP
YMHPPQYTITRLKEDNVGKSKKPSYSLIQQPEVHVPSSDKEPVSHDEPAVKSFAEHRVIKHPQTSFIKRLWTSLFGGHSV
AHTVTQEPGKKHHRAQNQGKHASSKSSGGDKRQQGSNANRRRRSGGGQQRSSSGNTNAAASTHQQRKKSNANQQSHGSQQ
SNRSIPAKNEGNKKREAILAKEPEEK

Specific function: Matures 5S rRNA from its precursors from all the rRNA genes. Major endoribonuclease participating in mRNA turnover. It initiates the decay of RNAs by cutting them internally near their 5'-end. It is able to remove poly(A) tails by an endonucleolytic proce

COG id: COG1530

COG function: function code J; Ribonucleases G and E

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1787325, Length=540, Percent_Identity=58.1481481481482, Blast_Score=614, Evalue=1e-177,
Organism=Escherichia coli, GI87082235, Length=489, Percent_Identity=34.560327198364, Blast_Score=239, Evalue=4e-64,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR021968
- InterPro:   IPR003029
- InterPro:   IPR019307
- InterPro:   IPR022967
- InterPro:   IPR004659 [H]

Pfam domain/function: PF12111 PNPase_C; PF10150 RNase_E_G; PF00575 S1 [H]

EC number: =3.1.26.12 [H]

Molecular weight: Translated: 74884; Mature: 74753

Theoretical pI: Translated: 9.00; Mature: 9.00

Prosite motif: PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGLYMEKMLINAMQTEEIRVALIKNNQLFDLDIECPGEIKKKGNIYKAIVTRREPSLDAV
CCHHHHHHHHHHHCCCCEEEEEEECCEEEEEECCCCCHHHHCCCEEEEHHCCCCCCCCEE
FVEYGSKRQGFLPLKEIAPEYLSKNPEEFGDEKPPITSLIREGQELLIQVDKEERGNKGA
EEECCCCCCCCCCHHHHCHHHHCCCCHHHCCCCCCHHHHHHCCHHHEEEECCCCCCCCCC
ALTTFITLAGCYLVLMPNNPHSGGISRRIEGDERDELRETLNALTLPEDMGLIIRTAGVG
HHHHHHHHHCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEECCCC
KSLEELQDDLNMLCNQWQSIKEAYNSELAPCLIHQEGDVIIRSIRDNLRKSISEIIIDDQ
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHCH
ISYIKAKQYIERVKPEFLPNLKLYNSSIPLFNFYQIESQIETAYQRQVMLPSGGALVIDR
HHHHHHHHHHHHHCHHHCCCCEEECCCCCEEEHHHHHHHHHHHHHHEEECCCCCEEEEEC
TEALVSIDINSAKATGGADIEATALNTNLEAADEIARQLRLRDLGGLVVIDFIDMSSSKN
CCEEEEEECCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCC
QREVENRLKEALQADRARIQVGRISRFGLLEMSRQRLRLSLGETAQEICPRCEGRGTVRS
HHHHHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHH
IQSHGLSITRLIEEEALKEKTAEIQVQLPPEMATFIMNEKRNFIRDIEKRHGVSVVVIAN
HHHCCCHHHHHHHHHHHHHHCCEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEC
PYMHPPQYTITRLKEDNVGKSKKPSYSLIQQPEVHVPSSDKEPVSHDEPAVKSFAEHRVI
CCCCCCCHHHHEECCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
KHPQTSFIKRLWTSLFGGHSVAHTVTQEPGKKHHRAQNQGKHASSKSSGGDKRQQGSNAN
CCCHHHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCCHHHCCCCCH
RRRRSGGGQQRSSSGNTNAAASTHQQRKKSNANQQSHGSQQSNRSIPAKNEGNKKREAIL
HHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHCCCCHHCCCCCCCCCCCHHHHHHH
AKEPEEK
CCCCCCC
>Mature Secondary Structure 
GLYMEKMLINAMQTEEIRVALIKNNQLFDLDIECPGEIKKKGNIYKAIVTRREPSLDAV
CHHHHHHHHHHHCCCCEEEEEEECCEEEEEECCCCCHHHHCCCEEEEHHCCCCCCCCEE
FVEYGSKRQGFLPLKEIAPEYLSKNPEEFGDEKPPITSLIREGQELLIQVDKEERGNKGA
EEECCCCCCCCCCHHHHCHHHHCCCCHHHCCCCCCHHHHHHCCHHHEEEECCCCCCCCCC
ALTTFITLAGCYLVLMPNNPHSGGISRRIEGDERDELRETLNALTLPEDMGLIIRTAGVG
HHHHHHHHHCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEECCCC
KSLEELQDDLNMLCNQWQSIKEAYNSELAPCLIHQEGDVIIRSIRDNLRKSISEIIIDDQ
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHCH
ISYIKAKQYIERVKPEFLPNLKLYNSSIPLFNFYQIESQIETAYQRQVMLPSGGALVIDR
HHHHHHHHHHHHHCHHHCCCCEEECCCCCEEEHHHHHHHHHHHHHHEEECCCCCEEEEEC
TEALVSIDINSAKATGGADIEATALNTNLEAADEIARQLRLRDLGGLVVIDFIDMSSSKN
CCEEEEEECCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCC
QREVENRLKEALQADRARIQVGRISRFGLLEMSRQRLRLSLGETAQEICPRCEGRGTVRS
HHHHHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHH
IQSHGLSITRLIEEEALKEKTAEIQVQLPPEMATFIMNEKRNFIRDIEKRHGVSVVVIAN
HHHCCCHHHHHHHHHHHHHHCCEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEC
PYMHPPQYTITRLKEDNVGKSKKPSYSLIQQPEVHVPSSDKEPVSHDEPAVKSFAEHRVI
CCCCCCCHHHHEECCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
KHPQTSFIKRLWTSLFGGHSVAHTVTQEPGKKHHRAQNQGKHASSKSSGGDKRQQGSNAN
CCCHHHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCCHHHCCCCCH
RRRRSGGGQQRSSSGNTNAAASTHQQRKKSNANQQSHGSQQSNRSIPAKNEGNKKREAIL
HHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHCCCCHHCCCCCCCCCCCHHHHHHH
AKEPEEK
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]