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The map label for this gene is menE [H]

Identifier: 52842443

GI number: 52842443

Start: 2521730

End: 2523124

Strand: Direct

Name: menE [H]

Synonym: lpg2230

Alternate gene names: 52842443

Gene position: 2521730-2523124 (Clockwise)

Preceding gene: 52842442

Following gene: 52842444

Centisome position: 74.22

GC content: 39.21

Gene sequence:

>1395_bases
TTGAGGAATAAAACCATGCTGTTTTTGGCACCAGATGTTCCAAATAAAGTAGCAATCACTCAGGGCAATCGAGTAATAAC
CTTTGATGAATTAAGGCATGAAGTTTTAGCAAATTCAAAGCAACTCATGAAACTTCCAAAAACCATCATTGTCCTCCATG
CAACACCTGAGATTGGATTTATTATTCAACTATTGGCCTGTTTGGAAACAAATCGCCCCATTGCCCTTTTCCCCAATTCC
ATTTCAGAGGAAGAAAAGCAAAGACGGCTTTCTCTTTTAGGTAATGCCGTAATGATAAATGAAGAAGGGGAATTGCAGGA
AATATGTGAAAATAAGACGATCCAGCCCCATCCCCAGACAGCACTTATTCTCTTCACCTCAGGAAGCACAGGAGCAGTCA
AAGCAGTTCAACTATCAAGCATCAACATAAAAGCCAACTGCCATGCGGTCATAAAGGCGCTGGAATTTAAAAAAGTTCAA
GATCAATTGCTCTTTTTACCTTTATCCTATTCTTTTGGCCTTCTGGGCCAATTGCTCCCTGGTTTAATGTTTGGCCTGAC
CACTCGACTAATCACTCATTTTACAGACATTAAAATGCTAATGGAGCAAGGTACCGTTCCTCAAATGTGGAGTGGCGTAC
CTTCTCACTGGGTAGCTATCAATGCCATAGCAAAACGATACCCTGATAGCGCAGCCAAAATTAACGCGATTGTTTCTGCT
GGCGCTCCTTTATCCACTACACTCAGAGCGGATTTGAAACAAATATTTCCTAACGCTATCATCTATAACAATTACGGTTT
AACTGAAGCATCTCCGAGAGTGCTGACTTATTCCAGTAAGGATCCTTTATTCACAGAAAATTATGCAGGTTATCCTGTTG
GCGATTGGAAGGTCAAACTTTCTGCTGAACATGAATTATTAATTAGCGGCACTCAAATGATGCTGGGTTATCTTGGTGAA
AAAGACAGTACTAAAATACAAAATGGCTGGTTATCAACAGGAGATATTGCGGAAATATTACCCAATGGGTTAGTTGCAAT
CAAAGGACGTCGAGACAGTCTTGTTAATATTGGGGGAGAAAAAGTTAACCTCTCTGAGATCGAGCAAGCAATTTGCCAAA
TTGATGTAATAAAAGAGGCCATTGTTATCCCTCAGGAAGACAAGATATATGGGACACGATTAATTGTCTGTATCGAAAAA
AAATCGCTGCCTTCTTCCATTTCTGAACAAAATTTAATAGAAAAAATACAAACGCATCTACTACCCAGGAAATTGCCTAT
TCAATTACAGCTCATGTCATCCCTACCCAGAAATCAACATGGGAAGCTTGATAGAAAAGCGTTGGTTTTGAATATTGATG
GTTTAGATAATAAGGAAAAAAATAATGCTAAGTAA

Upstream 100 bases:

>100_bases
ATGCCACATACTACCAGCGGAAAAATTCGCAGGAATTTTTGTCGAAAACATCTTTTGGATAAAACTCTGCCAATAGTGGC
TACCTGGCAACTCAATAAAA

Downstream 100 bases:

>100_bases
TCGAATTGAACAAGTACTGGAACGAATTGGTCTTACCAATCTTCCTAAAAACAAACAGGCAAAACTTGAGCAAGGGGGCT
TGGATAGTTTGATGCTGGCC

Product: acyl CoA ligase

Products: NA

Alternate protein names: OSB-CoA synthetase; o-succinylbenzoyl-CoA synthetase [H]

Number of amino acids: Translated: 464; Mature: 464

Protein sequence:

>464_residues
MRNKTMLFLAPDVPNKVAITQGNRVITFDELRHEVLANSKQLMKLPKTIIVLHATPEIGFIIQLLACLETNRPIALFPNS
ISEEEKQRRLSLLGNAVMINEEGELQEICENKTIQPHPQTALILFTSGSTGAVKAVQLSSINIKANCHAVIKALEFKKVQ
DQLLFLPLSYSFGLLGQLLPGLMFGLTTRLITHFTDIKMLMEQGTVPQMWSGVPSHWVAINAIAKRYPDSAAKINAIVSA
GAPLSTTLRADLKQIFPNAIIYNNYGLTEASPRVLTYSSKDPLFTENYAGYPVGDWKVKLSAEHELLISGTQMMLGYLGE
KDSTKIQNGWLSTGDIAEILPNGLVAIKGRRDSLVNIGGEKVNLSEIEQAICQIDVIKEAIVIPQEDKIYGTRLIVCIEK
KSLPSSISEQNLIEKIQTHLLPRKLPIQLQLMSSLPRNQHGKLDRKALVLNIDGLDNKEKNNAK

Sequences:

>Translated_464_residues
MRNKTMLFLAPDVPNKVAITQGNRVITFDELRHEVLANSKQLMKLPKTIIVLHATPEIGFIIQLLACLETNRPIALFPNS
ISEEEKQRRLSLLGNAVMINEEGELQEICENKTIQPHPQTALILFTSGSTGAVKAVQLSSINIKANCHAVIKALEFKKVQ
DQLLFLPLSYSFGLLGQLLPGLMFGLTTRLITHFTDIKMLMEQGTVPQMWSGVPSHWVAINAIAKRYPDSAAKINAIVSA
GAPLSTTLRADLKQIFPNAIIYNNYGLTEASPRVLTYSSKDPLFTENYAGYPVGDWKVKLSAEHELLISGTQMMLGYLGE
KDSTKIQNGWLSTGDIAEILPNGLVAIKGRRDSLVNIGGEKVNLSEIEQAICQIDVIKEAIVIPQEDKIYGTRLIVCIEK
KSLPSSISEQNLIEKIQTHLLPRKLPIQLQLMSSLPRNQHGKLDRKALVLNIDGLDNKEKNNAK
>Mature_464_residues
MRNKTMLFLAPDVPNKVAITQGNRVITFDELRHEVLANSKQLMKLPKTIIVLHATPEIGFIIQLLACLETNRPIALFPNS
ISEEEKQRRLSLLGNAVMINEEGELQEICENKTIQPHPQTALILFTSGSTGAVKAVQLSSINIKANCHAVIKALEFKKVQ
DQLLFLPLSYSFGLLGQLLPGLMFGLTTRLITHFTDIKMLMEQGTVPQMWSGVPSHWVAINAIAKRYPDSAAKINAIVSA
GAPLSTTLRADLKQIFPNAIIYNNYGLTEASPRVLTYSSKDPLFTENYAGYPVGDWKVKLSAEHELLISGTQMMLGYLGE
KDSTKIQNGWLSTGDIAEILPNGLVAIKGRRDSLVNIGGEKVNLSEIEQAICQIDVIKEAIVIPQEDKIYGTRLIVCIEK
KSLPSSISEQNLIEKIQTHLLPRKLPIQLQLMSSLPRNQHGKLDRKALVLNIDGLDNKEKNNAK

Specific function: Converts 2-succinylbenzoate (OSB) to 2-succinylbenzoyl- CoA (OSB-CoA) [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family. MenE subfamily [H]

Homologues:

Organism=Homo sapiens, GI187761345, Length=380, Percent_Identity=26.3157894736842, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI187761343, Length=380, Percent_Identity=26.3157894736842, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI156151445, Length=359, Percent_Identity=27.2980501392758, Blast_Score=84, Evalue=4e-16,
Organism=Escherichia coli, GI1788107, Length=358, Percent_Identity=27.3743016759777, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI145693145, Length=351, Percent_Identity=25.0712250712251, Blast_Score=89, Evalue=8e-19,
Organism=Escherichia coli, GI1786801, Length=550, Percent_Identity=26, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1786810, Length=413, Percent_Identity=22.0338983050847, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1788595, Length=351, Percent_Identity=25.6410256410256, Blast_Score=65, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI17558820, Length=403, Percent_Identity=25.3101736972705, Blast_Score=109, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI17531443, Length=366, Percent_Identity=26.2295081967213, Blast_Score=93, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI32563687, Length=346, Percent_Identity=23.9884393063584, Blast_Score=88, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17557194, Length=360, Percent_Identity=25.8333333333333, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17538037, Length=360, Percent_Identity=25.5555555555556, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17559526, Length=365, Percent_Identity=25.7534246575342, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17560308, Length=300, Percent_Identity=25, Blast_Score=72, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI17556356, Length=242, Percent_Identity=27.2727272727273, Blast_Score=66, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6319699, Length=354, Percent_Identity=24.8587570621469, Blast_Score=82, Evalue=2e-16,
Organism=Drosophila melanogaster, GI18859661, Length=350, Percent_Identity=27.1428571428571, Blast_Score=86, Evalue=4e-17,
Organism=Drosophila melanogaster, GI24581924, Length=357, Percent_Identity=28.0112044817927, Blast_Score=82, Evalue=9e-16,
Organism=Drosophila melanogaster, GI21355181, Length=378, Percent_Identity=25.3968253968254, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24648676, Length=379, Percent_Identity=23.4828496042216, Blast_Score=76, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000873
- InterPro:   IPR010192
- InterPro:   IPR023015 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.26 [H]

Molecular weight: Translated: 51394; Mature: 51394

Theoretical pI: Translated: 8.87; Mature: 8.87

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRNKTMLFLAPDVPNKVAITQGNRVITFDELRHEVLANSKQLMKLPKTIIVLHATPEIGF
CCCCEEEEECCCCCCEEEEECCCEEEEHHHHHHHHHHCCHHHHHCCCEEEEEECCCCHHH
IIQLLACLETNRPIALFPNSISEEEKQRRLSLLGNAVMINEEGELQEICENKTIQPHPQT
HHHHHHHHCCCCCEEECCCCCCHHHHHHHHHHHCCEEEECCCCHHHHHHCCCCCCCCCCE
ALILFTSGSTGAVKAVQLSSINIKANCHAVIKALEFKKVQDQLLFLPLSYSFGLLGQLLP
EEEEEECCCCCCEEEEEEEEEEEECHHHHHHHHHHHHHHCCEEEEEECCCCHHHHHHHHH
GLMFGLTTRLITHFTDIKMLMEQGTVPQMWSGVPSHWVAINAIAKRYPDSAAKINAIVSA
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCHHHHHHHHHHHCCCCHHHHHHEEEC
GAPLSTTLRADLKQIFPNAIIYNNYGLTEASPRVLTYSSKDPLFTENYAGYPVGDWKVKL
CCCCHHHHHHHHHHHCCCEEEEECCCCCCCCCCEEEECCCCCCEECCCCCCCCCCEEEEE
SAEHELLISGTQMMLGYLGEKDSTKIQNGWLSTGDIAEILPNGLVAIKGRRDSLVNIGGE
ECCCEEEEECHHHHHHHCCCCCCCCCCCCCCCCCCHHHHCCCCEEEEECCCCCEEECCCC
KVNLSEIEQAICQIDVIKEAIVIPQEDKIYGTRLIVCIEKKSLPSSISEQNLIEKIQTHL
EECHHHHHHHHHHHHHHHHHHCCCCCCCEECEEEEEEEECCCCCCCCHHHHHHHHHHHHH
LPRKLPIQLQLMSSLPRNQHGKLDRKALVLNIDGLDNKEKNNAK
CCCCCCEEEEHHHCCCCCCCCCCCCEEEEEEECCCCCCCCCCCC
>Mature Secondary Structure
MRNKTMLFLAPDVPNKVAITQGNRVITFDELRHEVLANSKQLMKLPKTIIVLHATPEIGF
CCCCEEEEECCCCCCEEEEECCCEEEEHHHHHHHHHHCCHHHHHCCCEEEEEECCCCHHH
IIQLLACLETNRPIALFPNSISEEEKQRRLSLLGNAVMINEEGELQEICENKTIQPHPQT
HHHHHHHHCCCCCEEECCCCCCHHHHHHHHHHHCCEEEECCCCHHHHHHCCCCCCCCCCE
ALILFTSGSTGAVKAVQLSSINIKANCHAVIKALEFKKVQDQLLFLPLSYSFGLLGQLLP
EEEEEECCCCCCEEEEEEEEEEEECHHHHHHHHHHHHHHCCEEEEEECCCCHHHHHHHHH
GLMFGLTTRLITHFTDIKMLMEQGTVPQMWSGVPSHWVAINAIAKRYPDSAAKINAIVSA
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCHHHHHHHHHHHCCCCHHHHHHEEEC
GAPLSTTLRADLKQIFPNAIIYNNYGLTEASPRVLTYSSKDPLFTENYAGYPVGDWKVKL
CCCCHHHHHHHHHHHCCCEEEEECCCCCCCCCCEEEECCCCCCEECCCCCCCCCCEEEEE
SAEHELLISGTQMMLGYLGEKDSTKIQNGWLSTGDIAEILPNGLVAIKGRRDSLVNIGGE
ECCCEEEEECHHHHHHHCCCCCCCCCCCCCCCCCCHHHHCCCCEEEEECCCCCEEECCCC
KVNLSEIEQAICQIDVIKEAIVIPQEDKIYGTRLIVCIEKKSLPSSISEQNLIEKIQTHL
EECHHHHHHHHHHHHHHHHHHCCCCCCCEECEEEEEEEECCCCCCCCHHHHHHHHHHHHH
LPRKLPIQLQLMSSLPRNQHGKLDRKALVLNIDGLDNKEKNNAK
CCCCCCEEEEHHHCCCCCCCCCCCCEEEEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA