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The map label for this gene is fadD21 [H]

Identifier: 52842442

GI number: 52842442

Start: 2519995

End: 2521740

Strand: Direct

Name: fadD21 [H]

Synonym: lpg2229

Alternate gene names: 52842442

Gene position: 2519995-2521740 (Clockwise)

Preceding gene: 52842441

Following gene: 52842443

Centisome position: 74.17

GC content: 41.07

Gene sequence:

>1746_bases
GTGAAAAAAGAATATTTGCAGTGCCAGTCTCTGGTTGACGTCGTCAGGTTACGTGCTTTACACAGCCCTAACAAGAAAAG
CTGTACTTTTCTGAACAAAGAGTTGGAAGAGACGATGACTTATGAGCAACTGGATCAACACGCCAAAGCAATTGCGGCAA
CTTTGCAAGCAGAAGGAGCAAAACCTGGGGATAGGGTCTTGTTATTGTTTGCACCTGGATTACCCCTTATCCAGGCATTT
TTGGGCTGCCTTTATGCAGGCTGCATTGCTGTACCCATTTACCCACCAGCCCAAGAAAAATTATTGGACAAGGCACAACG
CATAGTTACCAACTCAAAACCGGTCATAGTACTGATGATTGCGGATCACATCAAAAAATTCACCGCAGACGAATTAAATA
CAAATCCCAAATTCCTGAAAATTCCTGCTATTGCGCTTGAGAGCATTGAGTTAAACAGAAGTAGTAGTTGGCAACCAACC
TCCATTAAGAGCAATGACATTGCGTTTCTGCAATACACTTCCGGCTCAACCATGCACCCTAAAGGAGTGATGGTAAGCCA
CCATAATTTACTGGATAATCTGAATAAAATTTTTACCTCTTTTCATATGAATGATGAAACCATTATTTTCAGCTGGCTGC
CCCCACATCATGATATGGGTTTGATTGGCTGCATTCTGACCCCCATCTATGGTGGAATTCAGGCAATCATGATGTCCCCT
TTCTCATTTTTACAAAACCCGCTTTCCTGGTTAAAACATATTACCAAATACAAAGCAACTATCAGTGGAAGCCCTAACTT
CGCTTACGATTATTGTGTCAAACGAATCAGGGAAGAAAAAAAAGAAGGGCTGGATTTAAGTTCATGGGTGACTGCTTTCA
ACGGTGCTGAGCCAGTACGAGAAGAAACCATGGAACATTTTTATCAGGCATTTAAAGAGTTTGGATTTCGTAAAGAAGCC
TTCTATCCATGCTATGGCCTGGCTGAGGCCACTTTGTTAGTGACGGGAGGAACACCAGGAAGTTCATACAAAACATTAAC
TCTGGCCAAAGAACAATTTCAAGATCATCGCGTGCATTTTGCAGACGATAACAGTCCAGGCAGTTACAAGTTAGTCAGCA
GTGGTAATCCCATTCAAGAAGTTAAAATTATAGATCCTGATACCTTGATCCCATGTGATTTTGACCAGGTTGGTGAAATT
TGGGTACAAAGTAACAGTGTCGCCAAAGGATATTGGAATCAACCCGAAGAAACAAGGCATGCGTTCGCAGGAAAAATTAA
AGACGATGAGCGTAGCGCAATCTATTTAAGAACCGGGGACTTGGGCTTTCTCCATGAAAATGAGTTATACGTTACTGGAC
GCATTAAAGACTTAATTATTATTTATGGTAAAAATCATTATCCTCAGGACATAGAGTTCAGCCTGATGCATTCTCCGCTC
CATCACGTATTGGGCAAATGCGCTGCTTTTGTGATTCAGGAGGAGCATGAATATAAACTGACTGTGATGTGTGAAGTAAA
AAATCGATTCATGGATGACGTAGCTCAAGACAATTTATTCAATGAGATTTTTGAGCTTGTTTACGAAAACCACCAATTGG
AGGTACATACTATAGTCCTGATTCCTCTTAAAGCAATGCCACATACTACCAGCGGAAAAATTCGCAGGAATTTTTGTCGA
AAACATCTTTTGGATAAAACTCTGCCAATAGTGGCTACCTGGCAACTCAATAAAATTGAGGAATAA

Upstream 100 bases:

>100_bases
CAGGGAAAATTCTCCTGATGAGTGTTGGTGGTGGTCTATCCTATGCGGGACAAGTCCTAAACTATCAAAAAGCATCATAT
AGCAATTAAGGTGTTTTATA

Downstream 100 bases:

>100_bases
AACCATGCTGTTTTTGGCACCAGATGTTCCAAATAAAGTAGCAATCACTCAGGGCAATCGAGTAATAACCTTTGATGAAT
TAAGGCATGAAGTTTTAGCA

Product: saframycin Mx1 synthetase B

Products: AMP; diphosphate +an acyl-CoA [C]

Alternate protein names: Acyl-CoA synthetase [H]

Number of amino acids: Translated: 581; Mature: 581

Protein sequence:

>581_residues
MKKEYLQCQSLVDVVRLRALHSPNKKSCTFLNKELEETMTYEQLDQHAKAIAATLQAEGAKPGDRVLLLFAPGLPLIQAF
LGCLYAGCIAVPIYPPAQEKLLDKAQRIVTNSKPVIVLMIADHIKKFTADELNTNPKFLKIPAIALESIELNRSSSWQPT
SIKSNDIAFLQYTSGSTMHPKGVMVSHHNLLDNLNKIFTSFHMNDETIIFSWLPPHHDMGLIGCILTPIYGGIQAIMMSP
FSFLQNPLSWLKHITKYKATISGSPNFAYDYCVKRIREEKKEGLDLSSWVTAFNGAEPVREETMEHFYQAFKEFGFRKEA
FYPCYGLAEATLLVTGGTPGSSYKTLTLAKEQFQDHRVHFADDNSPGSYKLVSSGNPIQEVKIIDPDTLIPCDFDQVGEI
WVQSNSVAKGYWNQPEETRHAFAGKIKDDERSAIYLRTGDLGFLHENELYVTGRIKDLIIIYGKNHYPQDIEFSLMHSPL
HHVLGKCAAFVIQEEHEYKLTVMCEVKNRFMDDVAQDNLFNEIFELVYENHQLEVHTIVLIPLKAMPHTTSGKIRRNFCR
KHLLDKTLPIVATWQLNKIEE

Sequences:

>Translated_581_residues
MKKEYLQCQSLVDVVRLRALHSPNKKSCTFLNKELEETMTYEQLDQHAKAIAATLQAEGAKPGDRVLLLFAPGLPLIQAF
LGCLYAGCIAVPIYPPAQEKLLDKAQRIVTNSKPVIVLMIADHIKKFTADELNTNPKFLKIPAIALESIELNRSSSWQPT
SIKSNDIAFLQYTSGSTMHPKGVMVSHHNLLDNLNKIFTSFHMNDETIIFSWLPPHHDMGLIGCILTPIYGGIQAIMMSP
FSFLQNPLSWLKHITKYKATISGSPNFAYDYCVKRIREEKKEGLDLSSWVTAFNGAEPVREETMEHFYQAFKEFGFRKEA
FYPCYGLAEATLLVTGGTPGSSYKTLTLAKEQFQDHRVHFADDNSPGSYKLVSSGNPIQEVKIIDPDTLIPCDFDQVGEI
WVQSNSVAKGYWNQPEETRHAFAGKIKDDERSAIYLRTGDLGFLHENELYVTGRIKDLIIIYGKNHYPQDIEFSLMHSPL
HHVLGKCAAFVIQEEHEYKLTVMCEVKNRFMDDVAQDNLFNEIFELVYENHQLEVHTIVLIPLKAMPHTTSGKIRRNFCR
KHLLDKTLPIVATWQLNKIEE
>Mature_581_residues
MKKEYLQCQSLVDVVRLRALHSPNKKSCTFLNKELEETMTYEQLDQHAKAIAATLQAEGAKPGDRVLLLFAPGLPLIQAF
LGCLYAGCIAVPIYPPAQEKLLDKAQRIVTNSKPVIVLMIADHIKKFTADELNTNPKFLKIPAIALESIELNRSSSWQPT
SIKSNDIAFLQYTSGSTMHPKGVMVSHHNLLDNLNKIFTSFHMNDETIIFSWLPPHHDMGLIGCILTPIYGGIQAIMMSP
FSFLQNPLSWLKHITKYKATISGSPNFAYDYCVKRIREEKKEGLDLSSWVTAFNGAEPVREETMEHFYQAFKEFGFRKEA
FYPCYGLAEATLLVTGGTPGSSYKTLTLAKEQFQDHRVHFADDNSPGSYKLVSSGNPIQEVKIIDPDTLIPCDFDQVGEI
WVQSNSVAKGYWNQPEETRHAFAGKIKDDERSAIYLRTGDLGFLHENELYVTGRIKDLIIIYGKNHYPQDIEFSLMHSPL
HHVLGKCAAFVIQEEHEYKLTVMCEVKNRFMDDVAQDNLFNEIFELVYENHQLEVHTIVLIPLKAMPHTTSGKIRRNFCR
KHLLDKTLPIVATWQLNKIEE

Specific function: Esterification, Concomitant With Transport, Of Exogenous Long-Chain Fatty Acids Into Metabolically Active CoA Thioesters For Subsequent Degradation Or Incorporation Into Phospholipids. [C]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI45827692, Length=602, Percent_Identity=27.5747508305648, Blast_Score=175, Evalue=1e-43,
Organism=Homo sapiens, GI225735629, Length=602, Percent_Identity=27.5747508305648, Blast_Score=175, Evalue=1e-43,
Organism=Homo sapiens, GI44888818, Length=596, Percent_Identity=26.510067114094, Blast_Score=168, Evalue=2e-41,
Organism=Homo sapiens, GI55749758, Length=514, Percent_Identity=27.8210116731518, Blast_Score=159, Evalue=5e-39,
Organism=Homo sapiens, GI225735625, Length=564, Percent_Identity=22.8723404255319, Blast_Score=122, Evalue=1e-27,
Organism=Homo sapiens, GI45827694, Length=514, Percent_Identity=22.9571984435798, Blast_Score=103, Evalue=5e-22,
Organism=Homo sapiens, GI45827696, Length=462, Percent_Identity=23.3766233766234, Blast_Score=97, Evalue=5e-20,
Organism=Homo sapiens, GI225735627, Length=462, Percent_Identity=23.3766233766234, Blast_Score=97, Evalue=6e-20,
Organism=Homo sapiens, GI45827698, Length=434, Percent_Identity=23.7327188940092, Blast_Score=86, Evalue=1e-16,
Organism=Escherichia coli, GI1788107, Length=517, Percent_Identity=26.6924564796905, Blast_Score=119, Evalue=7e-28,
Organism=Caenorhabditis elegans, GI193202679, Length=540, Percent_Identity=23.7037037037037, Blast_Score=137, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI17531443, Length=575, Percent_Identity=24.1739130434783, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24654656, Length=466, Percent_Identity=27.6824034334764, Blast_Score=154, Evalue=1e-37,
Organism=Drosophila melanogaster, GI24581924, Length=555, Percent_Identity=23.963963963964, Blast_Score=94, Evalue=3e-19,
Organism=Drosophila melanogaster, GI18859661, Length=552, Percent_Identity=23.0072463768116, Blast_Score=87, Evalue=5e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.2.1.3 [C]

Molecular weight: Translated: 66039; Mature: 66039

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKEYLQCQSLVDVVRLRALHSPNKKSCTFLNKELEETMTYEQLDQHAKAIAATLQAEGA
CCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
KPGDRVLLLFAPGLPLIQAFLGCLYAGCIAVPIYPPAQEKLLDKAQRIVTNSKPVIVLMI
CCCCEEEEEEECCHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHCCCCCEEEEEE
ADHIKKFTADELNTNPKFLKIPAIALESIELNRSSSWQPTSIKSNDIAFLQYTSGSTMHP
HHHHHHHHHHHCCCCCCEEEECCEEEEEEECCCCCCCCCCCCCCCCEEEEEECCCCCCCC
KGVMVSHHNLLDNLNKIFTSFHMNDETIIFSWLPPHHDMGLIGCILTPIYGGIQAIMMSP
CCEEEEHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCH
FSFLQNPLSWLKHITKYKATISGSPNFAYDYCVKRIREEKKEGLDLSSWVTAFNGAEPVR
HHHHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCHHH
EETMEHFYQAFKEFGFRKEAFYPCYGLAEATLLVTGGTPGSSYKTLTLAKEQFQDHRVHF
HHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEECCCCCCCCEEEEEEHHHHHCCCEEE
ADDNSPGSYKLVSSGNPIQEVKIIDPDTLIPCDFDQVGEIWVQSNSVAKGYWNQPEETRH
ECCCCCCCEEEEECCCCCEEEEEECCCCCCCCCHHHHCCEEEECCCEECCCCCCCHHHHH
AFAGKIKDDERSAIYLRTGDLGFLHENELYVTGRIKDLIIIYGKNHYPQDIEFSLMHSPL
HHCCCCCCCCCCEEEEEECCCCEEECCCEEEEEEEEEEEEEECCCCCCCCCCHHHHHHHH
HHVLGKCAAFVIQEEHEYKLTVMCEVKNRFMDDVAQDNLFNEIFELVYENHQLEVHTIVL
HHHHHHHHHHHEECCCCEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEE
IPLKAMPHTTSGKIRRNFCRKHLLDKTLPIVATWQLNKIEE
EEEECCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCC
>Mature Secondary Structure
MKKEYLQCQSLVDVVRLRALHSPNKKSCTFLNKELEETMTYEQLDQHAKAIAATLQAEGA
CCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
KPGDRVLLLFAPGLPLIQAFLGCLYAGCIAVPIYPPAQEKLLDKAQRIVTNSKPVIVLMI
CCCCEEEEEEECCHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHCCCCCEEEEEE
ADHIKKFTADELNTNPKFLKIPAIALESIELNRSSSWQPTSIKSNDIAFLQYTSGSTMHP
HHHHHHHHHHHCCCCCCEEEECCEEEEEEECCCCCCCCCCCCCCCCEEEEEECCCCCCCC
KGVMVSHHNLLDNLNKIFTSFHMNDETIIFSWLPPHHDMGLIGCILTPIYGGIQAIMMSP
CCEEEEHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCH
FSFLQNPLSWLKHITKYKATISGSPNFAYDYCVKRIREEKKEGLDLSSWVTAFNGAEPVR
HHHHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCHHH
EETMEHFYQAFKEFGFRKEAFYPCYGLAEATLLVTGGTPGSSYKTLTLAKEQFQDHRVHF
HHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEECCCCCCCCEEEEEEHHHHHCCCEEE
ADDNSPGSYKLVSSGNPIQEVKIIDPDTLIPCDFDQVGEIWVQSNSVAKGYWNQPEETRH
ECCCCCCCEEEEECCCCCEEEEEECCCCCCCCCHHHHCCEEEECCCEECCCCCCCHHHHH
AFAGKIKDDERSAIYLRTGDLGFLHENELYVTGRIKDLIIIYGKNHYPQDIEFSLMHSPL
HHCCCCCCCCCCEEEEEECCCCEEECCCEEEEEEEEEEEEEECCCCCCCCCCHHHHHHHH
HHVLGKCAAFVIQEEHEYKLTVMCEVKNRFMDDVAQDNLFNEIFELVYENHQLEVHTIVL
HHHHHHHHHHHEECCCCEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEE
IPLKAMPHTTSGKIRRNFCRKHLLDKTLPIVATWQLNKIEE
EEEECCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Salts [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0408 {octanoate}} 0.0125 {octanoate}} 0.0059 {octanoate}} 0.00072 {octanoate}} 0.0013 {undecanoate}} 0.0022 {pentadecanoate}} 0.053 {CoA}} 0.035 {CoA}} 0.034 {CoA}} 0.0034 {tridecanoate}} 0.002 {laurate}} 0.0019 {laurate}} 0.0

Substrates: ATP; a long-chain carboxylic acid; CoA [C]

Specific reaction: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate +an acyl-CoA [C]

General reaction: Acid-thiol ligation; Phosphorylation [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]