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The map label for this gene is spoT

Identifier: 52842226

GI number: 52842226

Start: 2247838

End: 2249985

Strand: Reverse

Name: spoT

Synonym: lpg2009

Alternate gene names: 52842226

Gene position: 2249985-2247838 (Counterclockwise)

Preceding gene: 52842227

Following gene: 52842225

Centisome position: 66.22

GC content: 41.71

Gene sequence:

>2148_bases
ATGTTGGATACTAGGGAGTACTGCGTGAGCTACTTTAAAGAGCTCGATGAAGAGCTTAAATGCTACCTTGAGCAGGCGCA
AATAGAGAAATGTTATCAAGCTTATTTGGTGGCTGAAAAAGCACACCATGGCCAAATGCGTCGTTCAGGTGAGCCTTATA
TTACACACCCTGTAGCAGCAGCCCTCATTCTGGCAAGAATGAGACTGGACTATCAAACTATCATGGCTACTCTGCTTCAT
GACGTCGTTGAAGATACCTCCATAAGTAAAGAAGAACTGGTTAATCAATTTGGTGAAGAAGTCACTGCCCTTGTTGATGG
CGTCACCAAACTCACCAAAATTAAATTTGAATCAAAGGCCGAAGCTCAAGCGGAAAATTTTCGCAAGATGGTTTTGGCTA
TGGTTAAGGACATTCGCGTTATTATCGTTAAATTGGCTGATCGCTTACATAATATGCGCACTTTAGGATCAATGCCCGCA
GCCAAGCGCAGACGAATTGCAATTGAAACGCTCGAGATTTATGCCCCTATTGCCAATCGTTTGGGTATGCATTCTATTTA
TATCGGCCTTGAGGATTTAGGGTTTCAAGCTTTATATCCTATGCGTTACAGGGCAATTAAATCTGCTGTTGAAAAATCTC
GGGGTAATCGAAGAGAGTTGACACAGACCATAGAGAAAGATTTGCAACACGCCTTATCGCAGTTGAACATTCCCTATGAG
CAAGTATTTGGCAGGCAAAAGCATTTATATAGTATTTATAGAAAAATGAAGCAAAAGAAAGCATCCTTTACAGAAATTAC
GGATGTGTTTGCATTTCGCATCATTACAGAAGACATTGACTCCTGTTATAGAATTTTGGGAGCTTTGCACTGTACTTATA
AGCCTGTACCACAACGCTTTAAAGACTATATCGGAATTCCCAAAGCCAATGGCTATCAGTCATTGCATACCACTTTGTTT
GGGCCTTTTGGTGTTCCTCTTGAGGTTCAAATACGCACAAGAGAGATGGATAAAGTAGCTGATAATGGTGTAGCAGCGCA
CTGGATCTATAAATCTTCCGGATTGGAAGTCAATGAAGCTCAACTTCGTGCCAGAGAGTGGGTTCAAAGTTTGCTTGAAA
TGCAAAGGAGCACAGGCAGTTCTCTTGAATTTATCGAAAACGTTAAAATAGATTTATTTCCTGATGAAGTATATGTTTTT
ACTCCTAAAGGGCACATTATGGAGTTGCCGAAAGGAGCCACTCCTGTGGATTTTGCTTACACGGTACACTCTGGAGTGGG
AAATAGTTGTGTTGCAGCGAAAGTGAACAGGCGTTTGGTTCCTTTAAGCATTCCTTTATCCAATGGTCAAACCGTAGAGA
TTATTACAGCCCCGGGAGCACACCCCAACCCGGCTTGGCTGAATTTTGTTGTGACTGGAAAGGCAAGATCAAATATTCGT
CATTTTCTGAAAAGCCAACAACACGCTGAGTCTATTGTTTTAGGCAAACGATTATTGGAGCAATCTTTGGCTGAATTATC
CAGTGATTACGCCAAAGTGCCTCCGGAAACATTACAGGCATTGCTCAGCGATTTACACTATAAAACGGTTGATGACTTAT
TGTATGCCATAGGAATAGGTAATCAGATGGCAATGGTTATTGCCAAGCGGTTGGTGGTTTCGCAAGACGCCAATGAATCG
GATAAGGGCAATAAAAAACCTTTGGCTATTAAAGGCACTGAAGGGATGGTTGTGCATTTTGGTGAATGCTGCCAACCAAT
ACCTGGAGATAATATAGTTGGTAAATTTCAACAAGGCAGAGGCATTATTGTTCATGCCAGTGACTGCACTACACTAAAAA
CAACTCGTAGTAATCCGGAACAGTTTATTGCTTTGCGTTGGGATGAGCAGGTTCAGGGTGAATATTGGGTAGACATTACT
GTAGATGTAGCCAACGAAAGAGGTGTATTGGCTGCTTTAGCAACTGCTATTTCAGAAGCAGACTCTAATATAGGGAATAT
TAATGTGGATCCCAGAGATGGTCGTCATAATGCGGTCACTTTTTCCATCAGTGTTCGCGACAGAACCCATTTGGCTCGAG
TCATGCGTCGATTAAGGGCAAATAAAGTAGTTATGCGGTTATATCGAAAAAAACAAGGGGAGATTTAA

Upstream 100 bases:

>100_bases
ATATACCTCTTGGTAATCAAGTTATGGTATTTAAGCAAAGCAAAGGGAACAAGGGCGAGGCATAAAACCGTATTTTGAGT
AATGTGAGGTATAAATAGCC

Downstream 100 bases:

>100_bases
TGCAACCAATCAATACAAAATTGGCGCCAAAAGCGATAGGCACGTACAGTCAAGCGATAAAAAGTGGTGAGATCGTTTTT
CTTTCAGGGCAGATCCCATT

Product: guanosine-3, 5-bis(diphosphate)-3-pyrophosphohydrolase

Products: NA

Alternate protein names: Penta-phosphate guanosine-3'-pyrophosphohydrolase; (ppGpp)ase [H]

Number of amino acids: Translated: 715; Mature: 715

Protein sequence:

>715_residues
MLDTREYCVSYFKELDEELKCYLEQAQIEKCYQAYLVAEKAHHGQMRRSGEPYITHPVAAALILARMRLDYQTIMATLLH
DVVEDTSISKEELVNQFGEEVTALVDGVTKLTKIKFESKAEAQAENFRKMVLAMVKDIRVIIVKLADRLHNMRTLGSMPA
AKRRRIAIETLEIYAPIANRLGMHSIYIGLEDLGFQALYPMRYRAIKSAVEKSRGNRRELTQTIEKDLQHALSQLNIPYE
QVFGRQKHLYSIYRKMKQKKASFTEITDVFAFRIITEDIDSCYRILGALHCTYKPVPQRFKDYIGIPKANGYQSLHTTLF
GPFGVPLEVQIRTREMDKVADNGVAAHWIYKSSGLEVNEAQLRAREWVQSLLEMQRSTGSSLEFIENVKIDLFPDEVYVF
TPKGHIMELPKGATPVDFAYTVHSGVGNSCVAAKVNRRLVPLSIPLSNGQTVEIITAPGAHPNPAWLNFVVTGKARSNIR
HFLKSQQHAESIVLGKRLLEQSLAELSSDYAKVPPETLQALLSDLHYKTVDDLLYAIGIGNQMAMVIAKRLVVSQDANES
DKGNKKPLAIKGTEGMVVHFGECCQPIPGDNIVGKFQQGRGIIVHASDCTTLKTTRSNPEQFIALRWDEQVQGEYWVDIT
VDVANERGVLAALATAISEADSNIGNINVDPRDGRHNAVTFSISVRDRTHLARVMRRLRANKVVMRLYRKKQGEI

Sequences:

>Translated_715_residues
MLDTREYCVSYFKELDEELKCYLEQAQIEKCYQAYLVAEKAHHGQMRRSGEPYITHPVAAALILARMRLDYQTIMATLLH
DVVEDTSISKEELVNQFGEEVTALVDGVTKLTKIKFESKAEAQAENFRKMVLAMVKDIRVIIVKLADRLHNMRTLGSMPA
AKRRRIAIETLEIYAPIANRLGMHSIYIGLEDLGFQALYPMRYRAIKSAVEKSRGNRRELTQTIEKDLQHALSQLNIPYE
QVFGRQKHLYSIYRKMKQKKASFTEITDVFAFRIITEDIDSCYRILGALHCTYKPVPQRFKDYIGIPKANGYQSLHTTLF
GPFGVPLEVQIRTREMDKVADNGVAAHWIYKSSGLEVNEAQLRAREWVQSLLEMQRSTGSSLEFIENVKIDLFPDEVYVF
TPKGHIMELPKGATPVDFAYTVHSGVGNSCVAAKVNRRLVPLSIPLSNGQTVEIITAPGAHPNPAWLNFVVTGKARSNIR
HFLKSQQHAESIVLGKRLLEQSLAELSSDYAKVPPETLQALLSDLHYKTVDDLLYAIGIGNQMAMVIAKRLVVSQDANES
DKGNKKPLAIKGTEGMVVHFGECCQPIPGDNIVGKFQQGRGIIVHASDCTTLKTTRSNPEQFIALRWDEQVQGEYWVDIT
VDVANERGVLAALATAISEADSNIGNINVDPRDGRHNAVTFSISVRDRTHLARVMRRLRANKVVMRLYRKKQGEI
>Mature_715_residues
MLDTREYCVSYFKELDEELKCYLEQAQIEKCYQAYLVAEKAHHGQMRRSGEPYITHPVAAALILARMRLDYQTIMATLLH
DVVEDTSISKEELVNQFGEEVTALVDGVTKLTKIKFESKAEAQAENFRKMVLAMVKDIRVIIVKLADRLHNMRTLGSMPA
AKRRRIAIETLEIYAPIANRLGMHSIYIGLEDLGFQALYPMRYRAIKSAVEKSRGNRRELTQTIEKDLQHALSQLNIPYE
QVFGRQKHLYSIYRKMKQKKASFTEITDVFAFRIITEDIDSCYRILGALHCTYKPVPQRFKDYIGIPKANGYQSLHTTLF
GPFGVPLEVQIRTREMDKVADNGVAAHWIYKSSGLEVNEAQLRAREWVQSLLEMQRSTGSSLEFIENVKIDLFPDEVYVF
TPKGHIMELPKGATPVDFAYTVHSGVGNSCVAAKVNRRLVPLSIPLSNGQTVEIITAPGAHPNPAWLNFVVTGKARSNIR
HFLKSQQHAESIVLGKRLLEQSLAELSSDYAKVPPETLQALLSDLHYKTVDDLLYAIGIGNQMAMVIAKRLVVSQDANES
DKGNKKPLAIKGTEGMVVHFGECCQPIPGDNIVGKFQQGRGIIVHASDCTTLKTTRSNPEQFIALRWDEQVQGEYWVDIT
VDVANERGVLAALATAISEADSNIGNINVDPRDGRHNAVTFSISVRDRTHLARVMRRLRANKVVMRLYRKKQGEI

Specific function: In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes the degradation of ppGpp into G

COG id: COG0317

COG function: function code TK; Guanosine polyphosphate pyrophosphohydrolases/synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HD domain [H]

Homologues:

Organism=Escherichia coli, GI1790082, Length=700, Percent_Identity=53.1428571428571, Blast_Score=785, Evalue=0.0,
Organism=Escherichia coli, GI1789147, Length=659, Percent_Identity=35.660091047041, Blast_Score=398, Evalue=1e-112,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012675
- InterPro:   IPR003607
- InterPro:   IPR007685
- InterPro:   IPR004811
- InterPro:   IPR004095
- InterPro:   IPR012676 [H]

Pfam domain/function: PF04607 RelA_SpoT; PF02824 TGS [H]

EC number: =3.1.7.2 [H]

Molecular weight: Translated: 80672; Mature: 80672

Theoretical pI: Translated: 8.99; Mature: 8.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDTREYCVSYFKELDEELKCYLEQAQIEKCYQAYLVAEKAHHGQMRRSGEPYITHPVAA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCEEHHHHH
ALILARMRLDYQTIMATLLHDVVEDTSISKEELVNQFGEEVTALVDGVTKLTKIKFESKA
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
EAQAENFRKMVLAMVKDIRVIIVKLADRLHNMRTLGSMPAAKRRRIAIETLEIYAPIANR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
LGMHSIYIGLEDLGFQALYPMRYRAIKSAVEKSRGNRRELTQTIEKDLQHALSQLNIPYE
CCCCEEEEEHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCHH
QVFGRQKHLYSIYRKMKQKKASFTEITDVFAFRIITEDIDSCYRILGALHCTYKPVPQRF
HHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
KDYIGIPKANGYQSLHTTLFGPFGVPLEVQIRTREMDKVADNGVAAHWIYKSSGLEVNEA
HHHCCCCCCCCCHHHHHHHCCCCCCCEEEEEEHHHHHHHHCCCCEEEEEEECCCCCCHHH
QLRAREWVQSLLEMQRSTGSSLEFIENVKIDLFPDEVYVFTPKGHIMELPKGATPVDFAY
HHHHHHHHHHHHHHHHCCCCCHHHHHCCEEEEECCCEEEECCCCCEEECCCCCCCCEEEE
TVHSGVGNSCVAAKVNRRLVPLSIPLSNGQTVEIITAPGAHPNPAWLNFVVTGKARSNIR
EECCCCCCCHHHHHHCCEEEEEEEECCCCCEEEEEECCCCCCCCCEEEEEEECCHHHHHH
HFLKSQQHAESIVLGKRLLEQSLAELSSDYAKVPPETLQALLSDLHYKTVDDLLYAIGIG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCHHHHHHHHHHHCCC
NQMAMVIAKRLVVSQDANESDKGNKKPLAIKGTEGMVVHFGECCQPIPGDNIVGKFQQGR
CHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCEEEEHHHCCCCCCCCCHHHHCCCC
GIIVHASDCTTLKTTRSNPEQFIALRWDEQVQGEYWVDITVDVANERGVLAALATAISEA
EEEEECCCCCEEEECCCCCCCEEEEEECCCCCCCEEEEEEEEECCCCCHHHHHHHHHHHH
DSNIGNINVDPRDGRHNAVTFSISVRDRTHLARVMRRLRANKVVMRLYRKKQGEI
CCCCCCEECCCCCCCCCEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MLDTREYCVSYFKELDEELKCYLEQAQIEKCYQAYLVAEKAHHGQMRRSGEPYITHPVAA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCEEHHHHH
ALILARMRLDYQTIMATLLHDVVEDTSISKEELVNQFGEEVTALVDGVTKLTKIKFESKA
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
EAQAENFRKMVLAMVKDIRVIIVKLADRLHNMRTLGSMPAAKRRRIAIETLEIYAPIANR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
LGMHSIYIGLEDLGFQALYPMRYRAIKSAVEKSRGNRRELTQTIEKDLQHALSQLNIPYE
CCCCEEEEEHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCHH
QVFGRQKHLYSIYRKMKQKKASFTEITDVFAFRIITEDIDSCYRILGALHCTYKPVPQRF
HHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
KDYIGIPKANGYQSLHTTLFGPFGVPLEVQIRTREMDKVADNGVAAHWIYKSSGLEVNEA
HHHCCCCCCCCCHHHHHHHCCCCCCCEEEEEEHHHHHHHHCCCCEEEEEEECCCCCCHHH
QLRAREWVQSLLEMQRSTGSSLEFIENVKIDLFPDEVYVFTPKGHIMELPKGATPVDFAY
HHHHHHHHHHHHHHHHCCCCCHHHHHCCEEEEECCCEEEECCCCCEEECCCCCCCCEEEE
TVHSGVGNSCVAAKVNRRLVPLSIPLSNGQTVEIITAPGAHPNPAWLNFVVTGKARSNIR
EECCCCCCCHHHHHHCCEEEEEEEECCCCCEEEEEECCCCCCCCCEEEEEEECCHHHHHH
HFLKSQQHAESIVLGKRLLEQSLAELSSDYAKVPPETLQALLSDLHYKTVDDLLYAIGIG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCHHHHHHHHHHHCCC
NQMAMVIAKRLVVSQDANESDKGNKKPLAIKGTEGMVVHFGECCQPIPGDNIVGKFQQGR
CHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCEEEEHHHCCCCCCCCCHHHHCCCC
GIIVHASDCTTLKTTRSNPEQFIALRWDEQVQGEYWVDITVDVANERGVLAALATAISEA
EEEEECCCCCEEEECCCCCCCEEEEEECCCCCCCEEEEEEEEECCCCCHHHHHHHHHHHH
DSNIGNINVDPRDGRHNAVTFSISVRDRTHLARVMRRLRANKVVMRLYRKKQGEI
CCCCCCEECCCCCCCCCEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]