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The map label for this gene is gltX

Identifier: 52842129

GI number: 52842129

Start: 2132087

End: 2133571

Strand: Reverse

Name: gltX

Synonym: lpg1911

Alternate gene names: 52842129

Gene position: 2133571-2132087 (Counterclockwise)

Preceding gene: 52842132

Following gene: 52842128

Centisome position: 62.79

GC content: 40.07

Gene sequence:

>1485_bases
TTGAATCTTTGCGTTCATGGTAAACTCGGCTATTATAACTTGATTATTTTATTCCTTACAATGGAAGATATTATGACTGT
TAGAACCCGATTTGCCCCAAGCCCTACTGGTTTTTTACACGTTGGTGGGGTAAGGACTGCATTATTTTCATGGCTATATG
CAAAACATCACAATGGGCAATTTATACTGCGTATAGAAGATACGGACAGAGAGCGTTCAACTCAAGAATCTGTACAGGCT
ATTTTAGACGGAATGGCCTGGTTAGGGCTTAACTTCGATGAAGGTCCTTATTATCAGACTGAGCGATATGCTCGTTATCA
ACAAGTAGCGCAGCAACTTTTGGAAGAAGGAAAGGCTTATCGCTGTCAATGCAGTAAGGAAAGACTGGAAGCTTTAAGAG
AGGCTCAATTAGCCGCCAAGGAAAAGCCACGCTATGACGGACATTGCAGAAACCAAAGTCTTCCTGATAGTGGAATTCCT
TATGTTATTCGCTTTAGAAATCCTGATGCGGGTATAGTGTCTTTCCACGATGAGGTTTATGGTGACATTCATGTGGATAA
TAGCGAACTGGATGATTTGATTTTGGTTCGTTCTGATGGGCATCCCACCTATAATTTTGCTGTAGTTATCGATGATTGGG
ATATGAAAATAACTCATGTTATTCGCGGGGATGATCATATCAATAATACTCCGAGACAAATTAATTTATTCAAGGCATTG
GATGCACCGGTACCTGTTTTTGCCCATTTACCCATGATTTTGGGTGAAGATGGCAAACGGCTGTCAAAACGACATGGTGC
AGTAAGTGTCTTGCAGTTCAAGGAGCTGGGTGTATTGCCTCATGCTTTATTGAATTATTTAGTTCGTTTAGGATGGTCTC
ATGGCGATCAGGAAATTTTTAGTGTACAAGAAATGATAACCAGCTTTGACTTGAAAAATGTCAGCCGAGGTGTCTCAAGT
TTCAATTATGATAAGTTGTATTGGTTGAATCAGCATTATCAAAAAAGTGACTCTCCAGAATCAGTTGCTAATGCGTTGCA
ATGGCATTTTGAGCAGGCAGGAATTGATTTGAATCAAGGTCCCGATTTGAAGGATTTGGTCGCAGTTCAGGCAGAGAGAT
GTAAGTCATTAGCAGAAATGTGTCAAATTAGTCAGTATTTTTATACAGATATCATTGAATATAATGAAGATGCTGTTAAA
AAACACTTGCGGCCAGTTGTTCTGGAGCCTTTAATGGTATTGCATGAGCGCCTAAAAGCGCTTGACGAATGGAAAAATGA
TAAAATTCAAGAATGCATTAATGATGTGAGCCTGCAATTTGATTTGAATTTAGGTAAAATCGCTCAACCGTTGCGTGTCG
CCGTGACCGGATCCGGAACATCTCCTTCCATTGACATGACATTGGCTTTGTTAGGTAAAAACAAATCAATCAAACGTCTT
GAAGACGCTTTGGAGAAAATACGAGCCAGAGCCAGTGTTGTTTAG

Upstream 100 bases:

>100_bases
AGCAAGGCGACTAAAAAGGCAGGAATAAGAGTAGTGATTCTTAGCTGATATTTAATACCAATACTTTTCAACATAGCTCC
TTTTAGTCTTAATTCAGCAG

Downstream 100 bases:

>100_bases
AATCTAAATATTTATTGATTTTAAGCTGAATGCAAAAAAATCTGGATTTGGAGAAGTATTCATATTTCAAGTGGGCAATG
AAAACTACAATTTCGCTGGA

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS [H]

Number of amino acids: Translated: 494; Mature: 494

Protein sequence:

>494_residues
MNLCVHGKLGYYNLIILFLTMEDIMTVRTRFAPSPTGFLHVGGVRTALFSWLYAKHHNGQFILRIEDTDRERSTQESVQA
ILDGMAWLGLNFDEGPYYQTERYARYQQVAQQLLEEGKAYRCQCSKERLEALREAQLAAKEKPRYDGHCRNQSLPDSGIP
YVIRFRNPDAGIVSFHDEVYGDIHVDNSELDDLILVRSDGHPTYNFAVVIDDWDMKITHVIRGDDHINNTPRQINLFKAL
DAPVPVFAHLPMILGEDGKRLSKRHGAVSVLQFKELGVLPHALLNYLVRLGWSHGDQEIFSVQEMITSFDLKNVSRGVSS
FNYDKLYWLNQHYQKSDSPESVANALQWHFEQAGIDLNQGPDLKDLVAVQAERCKSLAEMCQISQYFYTDIIEYNEDAVK
KHLRPVVLEPLMVLHERLKALDEWKNDKIQECINDVSLQFDLNLGKIAQPLRVAVTGSGTSPSIDMTLALLGKNKSIKRL
EDALEKIRARASVV

Sequences:

>Translated_494_residues
MNLCVHGKLGYYNLIILFLTMEDIMTVRTRFAPSPTGFLHVGGVRTALFSWLYAKHHNGQFILRIEDTDRERSTQESVQA
ILDGMAWLGLNFDEGPYYQTERYARYQQVAQQLLEEGKAYRCQCSKERLEALREAQLAAKEKPRYDGHCRNQSLPDSGIP
YVIRFRNPDAGIVSFHDEVYGDIHVDNSELDDLILVRSDGHPTYNFAVVIDDWDMKITHVIRGDDHINNTPRQINLFKAL
DAPVPVFAHLPMILGEDGKRLSKRHGAVSVLQFKELGVLPHALLNYLVRLGWSHGDQEIFSVQEMITSFDLKNVSRGVSS
FNYDKLYWLNQHYQKSDSPESVANALQWHFEQAGIDLNQGPDLKDLVAVQAERCKSLAEMCQISQYFYTDIIEYNEDAVK
KHLRPVVLEPLMVLHERLKALDEWKNDKIQECINDVSLQFDLNLGKIAQPLRVAVTGSGTSPSIDMTLALLGKNKSIKRL
EDALEKIRARASVV
>Mature_494_residues
MNLCVHGKLGYYNLIILFLTMEDIMTVRTRFAPSPTGFLHVGGVRTALFSWLYAKHHNGQFILRIEDTDRERSTQESVQA
ILDGMAWLGLNFDEGPYYQTERYARYQQVAQQLLEEGKAYRCQCSKERLEALREAQLAAKEKPRYDGHCRNQSLPDSGIP
YVIRFRNPDAGIVSFHDEVYGDIHVDNSELDDLILVRSDGHPTYNFAVVIDDWDMKITHVIRGDDHINNTPRQINLFKAL
DAPVPVFAHLPMILGEDGKRLSKRHGAVSVLQFKELGVLPHALLNYLVRLGWSHGDQEIFSVQEMITSFDLKNVSRGVSS
FNYDKLYWLNQHYQKSDSPESVANALQWHFEQAGIDLNQGPDLKDLVAVQAERCKSLAEMCQISQYFYTDIIEYNEDAVK
KHLRPVVLEPLMVLHERLKALDEWKNDKIQECINDVSLQFDLNLGKIAQPLRVAVTGSGTSPSIDMTLALLGKNKSIKRL
EDALEKIRARASVV

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) [H]

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI134288884, Length=491, Percent_Identity=33.8085539714868, Blast_Score=238, Evalue=9e-63,
Organism=Homo sapiens, GI4826960, Length=108, Percent_Identity=37.037037037037, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI62241042, Length=212, Percent_Identity=24.5283018867925, Blast_Score=70, Evalue=6e-12,
Organism=Escherichia coli, GI1788743, Length=467, Percent_Identity=59.5289079229122, Blast_Score=600, Evalue=1e-173,
Organism=Escherichia coli, GI226510925, Length=294, Percent_Identity=33.6734693877551, Blast_Score=150, Evalue=1e-37,
Organism=Escherichia coli, GI1786895, Length=108, Percent_Identity=29.6296296296296, Blast_Score=65, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI71993394, Length=481, Percent_Identity=34.7193347193347, Blast_Score=236, Evalue=3e-62,
Organism=Saccharomyces cerevisiae, GI6324540, Length=506, Percent_Identity=34.1897233201581, Blast_Score=265, Evalue=1e-71,
Organism=Saccharomyces cerevisiae, GI6324742, Length=220, Percent_Identity=28.1818181818182, Blast_Score=85, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6321192, Length=208, Percent_Identity=25.4807692307692, Blast_Score=74, Evalue=6e-14,
Organism=Drosophila melanogaster, GI24665321, Length=491, Percent_Identity=31.9755600814664, Blast_Score=258, Evalue=5e-69,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00749 tRNA-synt_1c [H]

EC number: =6.1.1.17 [H]

Molecular weight: Translated: 56371; Mature: 56371

Theoretical pI: Translated: 6.33; Mature: 6.33

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLCVHGKLGYYNLIILFLTMEDIMTVRTRFAPSPTGFLHVGGVRTALFSWLYAKHHNGQ
CCEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECHHHHHHHHHHHHEECCCE
FILRIEDTDRERSTQESVQAILDGMAWLGLNFDEGPYYQTERYARYQQVAQQLLEEGKAY
EEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCE
RCQCSKERLEALREAQLAAKEKPRYDGHCRNQSLPDSGIPYVIRFRNPDAGIVSFHDEVY
EEECCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCEEEECHHHC
GDIHVDNSELDDLILVRSDGHPTYNFAVVIDDWDMKITHVIRGDDHINNTPRQINLFKAL
CEEEECCCCCCCEEEEECCCCCCEEEEEEEECCCCEEEEEEECCCCCCCCCCCEEHHHHC
DAPVPVFAHLPMILGEDGKRLSKRHGAVSVLQFKELGVLPHALLNYLVRLGWSHGDQEIF
CCCCCHHHHCHHHCCCCCHHHHHHCCCEEEEHHHHHCCCHHHHHHHHHHHCCCCCHHHHH
SVQEMITSFDLKNVSRGVSSFNYDKLYWLNQHYQKSDSPESVANALQWHFEQAGIDLNQG
HHHHHHHHHHHHHHHHHHCCCCCCEEEEECHHHCCCCCHHHHHHHHHHHHHHCCCCCCCC
PDLKDLVAVQAERCKSLAEMCQISQYFYTDIIEYNEDAVKKHLRPVVLEPLMVLHERLKA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCHHHHHHHHHHHHHHHH
LDEWKNDKIQECINDVSLQFDLNLGKIAQPLRVAVTGSGTSPSIDMTLALLGKNKSIKRL
HHHHCCCHHHHHHHHCEEEEECCHHHHCCCEEEEEECCCCCCCCEEEEEEECCCCCHHHH
EDALEKIRARASVV
HHHHHHHHHHHCCC
>Mature Secondary Structure
MNLCVHGKLGYYNLIILFLTMEDIMTVRTRFAPSPTGFLHVGGVRTALFSWLYAKHHNGQ
CCEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECHHHHHHHHHHHHEECCCE
FILRIEDTDRERSTQESVQAILDGMAWLGLNFDEGPYYQTERYARYQQVAQQLLEEGKAY
EEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCE
RCQCSKERLEALREAQLAAKEKPRYDGHCRNQSLPDSGIPYVIRFRNPDAGIVSFHDEVY
EEECCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCEEEECHHHC
GDIHVDNSELDDLILVRSDGHPTYNFAVVIDDWDMKITHVIRGDDHINNTPRQINLFKAL
CEEEECCCCCCCEEEEECCCCCCEEEEEEEECCCCEEEEEEECCCCCCCCCCCEEHHHHC
DAPVPVFAHLPMILGEDGKRLSKRHGAVSVLQFKELGVLPHALLNYLVRLGWSHGDQEIF
CCCCCHHHHCHHHCCCCCHHHHHHCCCEEEEHHHHHCCCHHHHHHHHHHHCCCCCHHHHH
SVQEMITSFDLKNVSRGVSSFNYDKLYWLNQHYQKSDSPESVANALQWHFEQAGIDLNQG
HHHHHHHHHHHHHHHHHHCCCCCCEEEEECHHHCCCCCHHHHHHHHHHHHHHCCCCCCCC
PDLKDLVAVQAERCKSLAEMCQISQYFYTDIIEYNEDAVKKHLRPVVLEPLMVLHERLKA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCHHHHHHHHHHHHHHHH
LDEWKNDKIQECINDVSLQFDLNLGKIAQPLRVAVTGSGTSPSIDMTLALLGKNKSIKRL
HHHHCCCHHHHHHHHCEEEEECCHHHHCCCEEEEEECCCCCCCCEEEEEEECCCCCHHHH
EDALEKIRARASVV
HHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA