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The map label for this gene is fabI

Identifier: 52842081

GI number: 52842081

Start: 2076229

End: 2077035

Strand: Direct

Name: fabI

Synonym: lpg1854

Alternate gene names: 52842081

Gene position: 2076229-2077035 (Clockwise)

Preceding gene: 52842080

Following gene: 52842088

Centisome position: 61.11

GC content: 40.64

Gene sequence:

>807_bases
ATGGGAGGAGATACTATTGTGGGATTTTTAACTGGAAAAAAAGCACTTATTGTTGGATTGGCAAGCAACAGATCGATTGC
ATATGGCATTGCTAAAGCATTCCATAATCAAGGCGCTGAGTTGGCTTTTACTTACCAAAACGAAAAATTACAGTCACGTG
TTGAAACAATGGCATCGGAATTTAATTCAACTCTAGTTTTTCCTTGTGATGTTGCAAGTGATGAAGAAATAAAAGCCGTG
TTTGATAATTTGCGTAATCACTGGGATAAATTAGATATCCTGGTCCACTCTGTAGCTTATGCTCCTGCTGATCAGATCAG
TGGTGATTTTGTAGAATGTGCAAATAGAGAAGGATTTAGAATAGCTCATGATATTAGTGCCTATAGCCTCATAGGTTTGT
CTCAAGCCGCATTGCCTATGATGCTAGATACTCAGGGTTCAATTTTAACCTTAAGCTATTATGGGGCAGAAAAAGCCGTA
CCAAACTATAACGTTATGGGTGTTGCGAAGGCAAGCTTGGAAGCCTCTGTGCGCTATCTGGCAGCCAGCTTAGGATCCAG
AGGATTAAGAATTAATGCCATATCAGCTGGACCAATTAAAACTCTTGCCGCTGCTGGAATTAAAGATTTTCGTAAAATTC
ATGCTGCCTATGCAAACATTACTCCATTACAAAGAAATGTTACTGCTGATGAAGTCGGAAATACTGCTGCCTTCTTATGC
TCTGATCTGGCATCCGGGATTACTGGTGAAGTGCTACATGTTGATGCAGGATATCACGCAGTATCAGCGATGAGCGAACT
AGGTTAG

Upstream 100 bases:

>100_bases
GTTGATGTTTTATTAGGCTGTTTAAGAGCAACCTAATTGATATTGTGGTTTTAGTCCAGGATCGAAGTAACACTCACTTC
GCCCTGCTAAATTAAGAATA

Downstream 100 bases:

>100_bases
ATATATATTAGTCCGCTTTAAATTGCTGACTTAATTCTTAGTTGTACTTAAAAAAATTCAATAAAGCTAAATTATATTGG
GGCTGAGCAAATAGCGAGGC

Product: enoyl reductase

Products: NA

Alternate protein names: NADH-dependent enoyl-ACP reductase [H]

Number of amino acids: Translated: 268; Mature: 267

Protein sequence:

>268_residues
MGGDTIVGFLTGKKALIVGLASNRSIAYGIAKAFHNQGAELAFTYQNEKLQSRVETMASEFNSTLVFPCDVASDEEIKAV
FDNLRNHWDKLDILVHSVAYAPADQISGDFVECANREGFRIAHDISAYSLIGLSQAALPMMLDTQGSILTLSYYGAEKAV
PNYNVMGVAKASLEASVRYLAASLGSRGLRINAISAGPIKTLAAAGIKDFRKIHAAYANITPLQRNVTADEVGNTAAFLC
SDLASGITGEVLHVDAGYHAVSAMSELG

Sequences:

>Translated_268_residues
MGGDTIVGFLTGKKALIVGLASNRSIAYGIAKAFHNQGAELAFTYQNEKLQSRVETMASEFNSTLVFPCDVASDEEIKAV
FDNLRNHWDKLDILVHSVAYAPADQISGDFVECANREGFRIAHDISAYSLIGLSQAALPMMLDTQGSILTLSYYGAEKAV
PNYNVMGVAKASLEASVRYLAASLGSRGLRINAISAGPIKTLAAAGIKDFRKIHAAYANITPLQRNVTADEVGNTAAFLC
SDLASGITGEVLHVDAGYHAVSAMSELG
>Mature_267_residues
GGDTIVGFLTGKKALIVGLASNRSIAYGIAKAFHNQGAELAFTYQNEKLQSRVETMASEFNSTLVFPCDVASDEEIKAVF
DNLRNHWDKLDILVHSVAYAPADQISGDFVECANREGFRIAHDISAYSLIGLSQAALPMMLDTQGSILTLSYYGAEKAVP
NYNVMGVAKASLEASVRYLAASLGSRGLRINAISAGPIKTLAAAGIKDFRKIHAAYANITPLQRNVTADEVGNTAAFLCS
DLASGITGEVLHVDAGYHAVSAMSELG

Specific function: Fatty acid biosynthesis pathway; second reduction step. [C]

COG id: COG0623

COG function: function code I; Enoyl-[acyl-carrier-protein] reductase (NADH)

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family. FabI subfamily [H]

Homologues:

Organism=Homo sapiens, GI59889578, Length=234, Percent_Identity=26.9230769230769, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI10190704, Length=268, Percent_Identity=26.1194029850746, Blast_Score=65, Evalue=6e-11,
Organism=Escherichia coli, GI1787545, Length=261, Percent_Identity=57.8544061302682, Blast_Score=321, Evalue=3e-89,
Organism=Escherichia coli, GI1787905, Length=253, Percent_Identity=29.2490118577075, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI87082100, Length=260, Percent_Identity=27.3076923076923, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1789378, Length=256, Percent_Identity=25.78125, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1787335, Length=262, Percent_Identity=29.0076335877863, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI2367365, Length=255, Percent_Identity=27.843137254902, Blast_Score=62, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI71994604, Length=258, Percent_Identity=25.1937984496124, Blast_Score=75, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI71994600, Length=259, Percent_Identity=25.0965250965251, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17536025, Length=272, Percent_Identity=27.2058823529412, Blast_Score=68, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI193204405, Length=269, Percent_Identity=25.6505576208178, Blast_Score=68, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI17560676, Length=261, Percent_Identity=25.2873563218391, Blast_Score=66, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17544670, Length=273, Percent_Identity=23.8095238095238, Blast_Score=65, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17561402, Length=264, Percent_Identity=25, Blast_Score=64, Evalue=7e-11,
Organism=Saccharomyces cerevisiae, GI6324126, Length=268, Percent_Identity=27.6119402985075, Blast_Score=64, Evalue=2e-11,
Organism=Drosophila melanogaster, GI21355319, Length=256, Percent_Identity=26.5625, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24644339, Length=257, Percent_Identity=26.8482490272374, Blast_Score=71, Evalue=7e-13,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR014358
- InterPro:   IPR002347
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: =1.3.1.9 [H]

Molecular weight: Translated: 28467; Mature: 28335

Theoretical pI: Translated: 5.88; Mature: 5.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGGDTIVGFLTGKKALIVGLASNRSIAYGIAKAFHNQGAELAFTYQNEKLQSRVETMASE
CCCCEEEEEEECCEEEEEEECCCCCHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHH
FNSTLVFPCDVASDEEIKAVFDNLRNHWDKLDILVHSVAYAPADQISGDFVECANREGFR
HCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCHHHHHCCCCCCE
IAHDISAYSLIGLSQAALPMMLDTQGSILTLSYYGAEKAVPNYNVMGVAKASLEASVRYL
EEECCCHHHHHCCCHHHCCEEEECCCCEEEEEECCCCCCCCCCCEEEEEHHHHHHHHHHH
AASLGSRGLRINAISAGPIKTLAAAGIKDFRKIHAAYANITPLQRNVTADEVGNTAAFLC
HHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCHHHHHH
SDLASGITGEVLHVDAGYHAVSAMSELG
HHHHCCCCCCEEEEECCHHHHHHHHHCC
>Mature Secondary Structure 
GGDTIVGFLTGKKALIVGLASNRSIAYGIAKAFHNQGAELAFTYQNEKLQSRVETMASE
CCCEEEEEEECCEEEEEEECCCCCHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHH
FNSTLVFPCDVASDEEIKAVFDNLRNHWDKLDILVHSVAYAPADQISGDFVECANREGFR
HCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCHHHHHCCCCCCE
IAHDISAYSLIGLSQAALPMMLDTQGSILTLSYYGAEKAVPNYNVMGVAKASLEASVRYL
EEECCCHHHHHCCCHHHCCEEEECCCCEEEEEECCCCCCCCCCCEEEEEHHHHHHHHHHH
AASLGSRGLRINAISAGPIKTLAAAGIKDFRKIHAAYANITPLQRNVTADEVGNTAAFLC
HHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCHHHHHH
SDLASGITGEVLHVDAGYHAVSAMSELG
HHHHCCCCCCEEEEECCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 10464225; 10984043 [H]