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The map label for this gene is bla [H]

Identifier: 52841846

GI number: 52841846

Start: 1786233

End: 1787075

Strand: Reverse

Name: bla [H]

Synonym: lpg1618

Alternate gene names: 52841846

Gene position: 1787075-1786233 (Counterclockwise)

Preceding gene: 52841847

Following gene: 52841844

Centisome position: 52.6

GC content: 37.96

Gene sequence:

>843_bases
ATGGGTTTGCATATCCATATATTCAAAAATGTTGAGGTTGTTATGAAGAAAATAATTATATTTTTGTGTACAGGATTATT
ATTTTGCGTCTCTACTTGGGCTCAAGGCACCTGTTTTTTAGCAAAAGAAAATCAAACTGTTTTAAAACGTGAGGGCAATG
ATTGCGATCAACGCTATTCGCCAGCATCAACTTTTAAAATTGCCCTTAGCCTCATGGGCTTTGATTCTGGAATATTAAAG
GATGAGTTGCATCCTGAATGGCCATACAAAAAAGAATATGAACTTTATCTGAATGTATGGAAGTACCCTCAAAACCCGCA
TACCTGGATCAGAGATTCCTGTGTTTGGTATTCACAGGCATTAACTCGACAATTGGGCATGAAACGGTTTAAAGGGTATG
TCGACGCATTTCACTACGGTAATCAGGATGTTTCGGGAGACAGAGGTCAAAATAACGGCTTAACCCATGCCTGGCTTTCC
AGCTCACTTTCTATCTCACCCACAGAGCAAATTCAATTTCTACAAAAAATAATCTACAAGAAACTACCGGTGAGTCAAAA
AGCTTATATCATGACTAAAAATATCATGTACATCCAGGAGTTGCCTGGTGGGTGGAAGCTTTATGGAAAAACAGGAACTG
GTCGGCAGTTAACAAAAGATAAAAGCCAAAAATTGCCTTTGCAACATGGATGGTTCGTAGGTTGGATTGAGAAGGATGAG
CGTGTAATTACCTTTGCAAAGCATATTGCAGATAGTAAAGAGAATAACACTTTTGCCAGTTTTAGAGCGAAAAATGATGC
TCTGATTCAATTGTTTAACCTCATTAATGAGTTGGAGAAATAA

Upstream 100 bases:

>100_bases
AAAAATGGAAAGACATTAGTTTCGGGAGGAGAATTGGCCGCACCGGTATTCCGAAATGTCGCAATGGATGCTCTGAGCAG
TGCAAATCGATAACTAGCTT

Downstream 100 bases:

>100_bases
TCTAAGACATTGTCGGAGCAGGATTATTCTGTTCCGATTACCCAAATCTGCCAACTATAGTCACACACATTCCAGGAGTT
GCTGCTGCTTGTCATCGGTA

Product: beta-lactamase AmpS

Products: NA

Alternate protein names: Penicillinase [H]

Number of amino acids: Translated: 280; Mature: 279

Protein sequence:

>280_residues
MGLHIHIFKNVEVVMKKIIIFLCTGLLFCVSTWAQGTCFLAKENQTVLKREGNDCDQRYSPASTFKIALSLMGFDSGILK
DELHPEWPYKKEYELYLNVWKYPQNPHTWIRDSCVWYSQALTRQLGMKRFKGYVDAFHYGNQDVSGDRGQNNGLTHAWLS
SSLSISPTEQIQFLQKIIYKKLPVSQKAYIMTKNIMYIQELPGGWKLYGKTGTGRQLTKDKSQKLPLQHGWFVGWIEKDE
RVITFAKHIADSKENNTFASFRAKNDALIQLFNLINELEK

Sequences:

>Translated_280_residues
MGLHIHIFKNVEVVMKKIIIFLCTGLLFCVSTWAQGTCFLAKENQTVLKREGNDCDQRYSPASTFKIALSLMGFDSGILK
DELHPEWPYKKEYELYLNVWKYPQNPHTWIRDSCVWYSQALTRQLGMKRFKGYVDAFHYGNQDVSGDRGQNNGLTHAWLS
SSLSISPTEQIQFLQKIIYKKLPVSQKAYIMTKNIMYIQELPGGWKLYGKTGTGRQLTKDKSQKLPLQHGWFVGWIEKDE
RVITFAKHIADSKENNTFASFRAKNDALIQLFNLINELEK
>Mature_279_residues
GLHIHIFKNVEVVMKKIIIFLCTGLLFCVSTWAQGTCFLAKENQTVLKREGNDCDQRYSPASTFKIALSLMGFDSGILKD
ELHPEWPYKKEYELYLNVWKYPQNPHTWIRDSCVWYSQALTRQLGMKRFKGYVDAFHYGNQDVSGDRGQNNGLTHAWLSS
SLSISPTEQIQFLQKIIYKKLPVSQKAYIMTKNIMYIQELPGGWKLYGKTGTGRQLTKDKSQKLPLQHGWFVGWIEKDER
VITFAKHIADSKENNTFASFRAKNDALIQLFNLINELEK

Specific function: Has a broad substrate profile, hydrolyzes amoxicillin, ticarcillin, cephalothin, ceftazidime, cefotaxime, and aztreonam, but not imipenem or cephamycins [H]

COG id: COG2602

COG function: function code V; Beta-lactamase class D

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-D beta-lactamase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR002137
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00905 Transpeptidase [H]

EC number: =3.5.2.6 [H]

Molecular weight: Translated: 32444; Mature: 32313

Theoretical pI: Translated: 9.60; Mature: 9.60

Prosite motif: PS00337 BETA_LACTAMASE_D

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGLHIHIFKNVEVVMKKIIIFLCTGLLFCVSTWAQGTCFLAKENQTVLKREGNDCDQRYS
CCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHCCCCCHHHHCC
PASTFKIALSLMGFDSGILKDELHPEWPYKKEYELYLNVWKYPQNPHTWIRDSCVWYSQA
CHHHHHHHHHHHCCCCCCHHCCCCCCCCCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHH
LTRQLGMKRFKGYVDAFHYGNQDVSGDRGQNNGLTHAWLSSSLSISPTEQIQFLQKIIYK
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHH
KLPVSQKAYIMTKNIMYIQELPGGWKLYGKTGTGRQLTKDKSQKLPLQHGWFVGWIEKDE
HCCCCCCEEEEECCEEEEEECCCCEEEEECCCCCCCCCCCHHHCCCCCCCCEEEEEECCC
RVITFAKHIADSKENNTFASFRAKNDALIQLFNLINELEK
HHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
GLHIHIFKNVEVVMKKIIIFLCTGLLFCVSTWAQGTCFLAKENQTVLKREGNDCDQRYS
CEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHCCCCCHHHHCC
PASTFKIALSLMGFDSGILKDELHPEWPYKKEYELYLNVWKYPQNPHTWIRDSCVWYSQA
CHHHHHHHHHHHCCCCCCHHCCCCCCCCCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHH
LTRQLGMKRFKGYVDAFHYGNQDVSGDRGQNNGLTHAWLSSSLSISPTEQIQFLQKIIYK
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHH
KLPVSQKAYIMTKNIMYIQELPGGWKLYGKTGTGRQLTKDKSQKLPLQHGWFVGWIEKDE
HCCCCCCEEEEECCEEEEEECCCCEEEEECCCCCCCCCCCHHHCCCCCCCCEEEEEECCC
RVITFAKHIADSKENNTFASFRAKNDALIQLFNLINELEK
HHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9333046 [H]