Click here to switch to the map view.

The map label for this gene is rpoE

Identifier: 52841807

GI number: 52841807

Start: 1742704

End: 1743267

Strand: Reverse

Name: rpoE

Synonym: lpg1577

Alternate gene names: 52841807

Gene position: 1743267-1742704 (Counterclockwise)

Preceding gene: 52841808

Following gene: 52841806

Centisome position: 51.31

GC content: 32.98

Gene sequence:

>564_bases
ATGCATATTGAGACCTCTTTAAGTGATGAATCTCTAATTGAATTAGCTCAAAAAGGCAACGGTTTAGCTTTTGATAAACT
GTTTGAGCGTTATCAGGGCAAAATTAGACAAATGATTTATTTTTACACGCATGACTGGACAAATGTCAATGATTTAACTC
AGGAAGTTCTTATCAAAGTATTTAGGCATTTACCTTATTTTAATGAACGCAGTCAGTTTTCAACCTGGCTATATAAAATT
ACTCAAAATACTATTAAAAATTATTTTCGTACCAATAATTTATATGCTGATTCAGAAATGCAGTATATGGATGAACAATT
TTCTACACCACATAATTCTCCAGAGTCAGACATAATGGGATTAGAGTTTGAGAAACAAATAGAAGCAGCAATGTCATGTT
TATCTGAAGAGTTACGATTATGTTATGGGCTGCACATATTTGATGGACAAACTTATCAGGATATTGCAAAAGAAATGCAT
TGCCCCATCGGAACTGTACGCTCACGTATATTTAGAGCTCGAAAACAGCTGATGGATATTGTAAGTGAGAATCACACTTT
ATAA

Upstream 100 bases:

>100_bases
TATATAATAATACATAATGATTAAAATAAATCCAAGTTAACAATGATTAGACTTTATCTTATCCTAACGTAATAACAAGC
AAAGGAATAATGAGGAAGCT

Downstream 100 bases:

>100_bases
TATCATTTTGATATCATATAAAATCATCAACCTTAATCGTGAAATCAATTGCATTTAATTATTAAATAAATAAGCTTACA
AAGATCTGCTATTAGATAAA

Product: RNA polymerase sigma E factor RpoE

Products: NA

Alternate protein names: Sigma-30 [H]

Number of amino acids: Translated: 187; Mature: 187

Protein sequence:

>187_residues
MHIETSLSDESLIELAQKGNGLAFDKLFERYQGKIRQMIYFYTHDWTNVNDLTQEVLIKVFRHLPYFNERSQFSTWLYKI
TQNTIKNYFRTNNLYADSEMQYMDEQFSTPHNSPESDIMGLEFEKQIEAAMSCLSEELRLCYGLHIFDGQTYQDIAKEMH
CPIGTVRSRIFRARKQLMDIVSENHTL

Sequences:

>Translated_187_residues
MHIETSLSDESLIELAQKGNGLAFDKLFERYQGKIRQMIYFYTHDWTNVNDLTQEVLIKVFRHLPYFNERSQFSTWLYKI
TQNTIKNYFRTNNLYADSEMQYMDEQFSTPHNSPESDIMGLEFEKQIEAAMSCLSEELRLCYGLHIFDGQTYQDIAKEMH
CPIGTVRSRIFRARKQLMDIVSENHTL
>Mature_187_residues
MHIETSLSDESLIELAQKGNGLAFDKLFERYQGKIRQMIYFYTHDWTNVNDLTQEVLIKVFRHLPYFNERSQFSTWLYKI
TQNTIKNYFRTNNLYADSEMQYMDEQFSTPHNSPESDIMGLEFEKQIEAAMSCLSEELRLCYGLHIFDGQTYQDIAKEMH
CPIGTVRSRIFRARKQLMDIVSENHTL

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor regulates genes such as algD, involved in alginate biosynthesis [H]

COG id: COG1595

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma24 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. ECF subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788926, Length=178, Percent_Identity=37.0786516853933, Blast_Score=114, Evalue=5e-27,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000838
- InterPro:   IPR007627
- InterPro:   IPR013249
- InterPro:   IPR014286
- InterPro:   IPR013325
- InterPro:   IPR013324 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF08281 Sigma70_r4_2 [H]

EC number: NA

Molecular weight: Translated: 22205; Mature: 22205

Theoretical pI: Translated: 5.57; Mature: 5.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHIETSLSDESLIELAQKGNGLAFDKLFERYQGKIRQMIYFYTHDWTNVNDLTQEVLIKV
CCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
FRHLPYFNERSQFSTWLYKITQNTIKNYFRTNNLYADSEMQYMDEQFSTPHNSPESDIMG
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCCC
LEFEKQIEAAMSCLSEELRLCYGLHIFDGQTYQDIAKEMHCPIGTVRSRIFRARKQLMDI
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
VSENHTL
HHCCCCC
>Mature Secondary Structure
MHIETSLSDESLIELAQKGNGLAFDKLFERYQGKIRQMIYFYTHDWTNVNDLTQEVLIKV
CCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
FRHLPYFNERSQFSTWLYKITQNTIKNYFRTNNLYADSEMQYMDEQFSTPHNSPESDIMG
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCCC
LEFEKQIEAAMSCLSEELRLCYGLHIFDGQTYQDIAKEMHCPIGTVRSRIFRARKQLMDI
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
VSENHTL
HHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8432708; 8378309; 7961421; 10984043; 7737518 [H]