Click here to switch to the map view.

The map label for this gene is bioB

Identifier: 52841702

GI number: 52841702

Start: 1626117

End: 1627064

Strand: Direct

Name: bioB

Synonym: lpg1472

Alternate gene names: 52841702

Gene position: 1626117-1627064 (Clockwise)

Preceding gene: 52841700

Following gene: 52841703

Centisome position: 47.86

GC content: 38.4

Gene sequence:

>948_bases
GTGTCTGAAGAAAAAAAACAGTGGTCAATTTCTGATATTGAAGCAATTTATCAACAACCATTTAATGATTTGCTTTATCA
AGCACATACCATTCATAGGACATATCACGATCCTAATTCTTTACAATTTGCAACCTTGTTAAGCATTAAAACGGGCGCAT
GCCCAGAAGATTGTGGCTATTGCTCTCAAAGTGGTCACTATAAAACGCATGTAGAAAAAGAAAAATTGATGTCAGTTGAA
GAGGTATTGCAATGTGCCAAAGAAGCAAAGGAGGGTGGGGCAAAACGTTTTTGTATGGGAGCTGCATGGCGTTGTCCACC
TGATAAAGCAATTCCGCAGCTAAAAGAAATGATAGAAGGAGTAAAATCTTTAGGGCTTGAAACCTGTATGACTTTAGGCA
TGCTTACCAAAGAACAGGCCTCTCATTTAAAGGAGGCTGGATTGGATTATTATAACCATAATATTGATACCTCACCATCC
TATTACGATAAGGTGGTAACCACTCGAAAATTCAGTGATCGATTAGATACTTTAAATAACGTTCGTTCAGCGGGAATTAA
TGTATGTTGTGGTGGTATTTTAGGATTGGGGGAAACCAGAGAAGATCGAATCGAATTTTTATTAACATTAGCTAATATGG
AAACTCCTCCGGAAAGTGTGCCAATTAATCGTTTAATTCCAGTGGAGGGAACACCTTTAGCCCAAGCTGAGAGAGTAGAA
GGGATTGAATTGGTAAGAACCATTGCTACAGCAAGAATTTTAATGCCTAAAAGTGCTATTAGATTGACAGCTGGTCGAAC
CGAAATGAGCGACGAGTTACAAGCACTTTGCTATTTTGCCGGAGCTAATTCTGTGTTTATTGGCGATAAATTATTGACAG
AAGATAATCCCCAGCGCTTCAAAGACAAAACTCTTTTTAACAAACTGGGTTTGACTGAGATGGTATAA

Upstream 100 bases:

>100_bases
TTGCAATAAGTTGATCTGTGTTTACCATTTTAAAAATTTTGGTTGACAAGTATAGGAGCGACTTTATCATGTTCACTATT
CTGAATTAAAGGGGGTAGCC

Downstream 100 bases:

>100_bases
ATGACTCTTAATATAAAAATTAATGAGTATACAGCACAGCTGCAAGAACAAGGATTATTGCGTACAAGACAAATTTTAGA
AACGGATAAGATAGATTATA

Product: biotin synthase BioB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 315; Mature: 314

Protein sequence:

>315_residues
MSEEKKQWSISDIEAIYQQPFNDLLYQAHTIHRTYHDPNSLQFATLLSIKTGACPEDCGYCSQSGHYKTHVEKEKLMSVE
EVLQCAKEAKEGGAKRFCMGAAWRCPPDKAIPQLKEMIEGVKSLGLETCMTLGMLTKEQASHLKEAGLDYYNHNIDTSPS
YYDKVVTTRKFSDRLDTLNNVRSAGINVCCGGILGLGETREDRIEFLLTLANMETPPESVPINRLIPVEGTPLAQAERVE
GIELVRTIATARILMPKSAIRLTAGRTEMSDELQALCYFAGANSVFIGDKLLTEDNPQRFKDKTLFNKLGLTEMV

Sequences:

>Translated_315_residues
MSEEKKQWSISDIEAIYQQPFNDLLYQAHTIHRTYHDPNSLQFATLLSIKTGACPEDCGYCSQSGHYKTHVEKEKLMSVE
EVLQCAKEAKEGGAKRFCMGAAWRCPPDKAIPQLKEMIEGVKSLGLETCMTLGMLTKEQASHLKEAGLDYYNHNIDTSPS
YYDKVVTTRKFSDRLDTLNNVRSAGINVCCGGILGLGETREDRIEFLLTLANMETPPESVPINRLIPVEGTPLAQAERVE
GIELVRTIATARILMPKSAIRLTAGRTEMSDELQALCYFAGANSVFIGDKLLTEDNPQRFKDKTLFNKLGLTEMV
>Mature_314_residues
SEEKKQWSISDIEAIYQQPFNDLLYQAHTIHRTYHDPNSLQFATLLSIKTGACPEDCGYCSQSGHYKTHVEKEKLMSVEE
VLQCAKEAKEGGAKRFCMGAAWRCPPDKAIPQLKEMIEGVKSLGLETCMTLGMLTKEQASHLKEAGLDYYNHNIDTSPSY
YDKVVTTRKFSDRLDTLNNVRSAGINVCCGGILGLGETREDRIEFLLTLANMETPPESVPINRLIPVEGTPLAQAERVEG
IELVRTIATARILMPKSAIRLTAGRTEMSDELQALCYFAGANSVFIGDKLLTEDNPQRFKDKTLFNKLGLTEMV

Specific function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism

COG id: COG0502

COG function: function code H; Biotin synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Biotin synthase family

Homologues:

Organism=Escherichia coli, GI1786992, Length=307, Percent_Identity=56.3517915309446, Blast_Score=389, Evalue=1e-109,
Organism=Saccharomyces cerevisiae, GI6321725, Length=322, Percent_Identity=47.2049689440994, Blast_Score=320, Evalue=3e-88,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BIOB_LEGPA (Q5X592)

Other databases:

- EMBL:   CR628336
- RefSeq:   YP_123752.1
- HSSP:   P12996
- ProteinModelPortal:   Q5X592
- SMR:   Q5X592
- STRING:   Q5X592
- GeneID:   3117229
- GenomeReviews:   CR628336_GR
- KEGG:   lpp:lpp1428
- LegioList:   lpp1428
- eggNOG:   COG0502
- HOGENOM:   HBG405569
- OMA:   MGMETCM
- ProtClustDB:   CLSK2390976
- BioCyc:   LPNE297246:LPP1428-MONOMER
- HAMAP:   MF_01694
- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR002684
- InterPro:   IPR006638
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00876
- SMART:   SM00729
- TIGRFAMs:   TIGR00433

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM

EC number: =2.8.1.6

Molecular weight: Translated: 35178; Mature: 35046

Theoretical pI: Translated: 5.69; Mature: 5.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEEKKQWSISDIEAIYQQPFNDLLYQAHTIHRTYHDPNSLQFATLLSIKTGACPEDCGY
CCCCHHCCCHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCHHHEEEECCCCCCHHHCC
CSQSGHYKTHVEKEKLMSVEEVLQCAKEAKEGGAKRFCMGAAWRCPPDKAIPQLKEMIEG
CCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHCCCCEEHHCCCCCCCCHHHHHHHHHHHHH
VKSLGLETCMTLGMLTKEQASHLKEAGLDYYNHNIDTSPSYYDKVVTTRKFSDRLDTLNN
HHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
VRSAGINVCCGGILGLGETREDRIEFLLTLANMETPPESVPINRLIPVEGTPLAQAERVE
HHHCCCHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCEECCCCCCCHHHHHHH
GIELVRTIATARILMPKSAIRLTAGRTEMSDELQALCYFAGANSVFIGDKLLTEDNPQRF
HHHHHHHHHHHHHHCCCHHHEEECCCCHHHHHHHHHHHHCCCCEEEECCCHHCCCCHHHH
KDKTLFNKLGLTEMV
HHHHHHHHCCHHHCC
>Mature Secondary Structure 
SEEKKQWSISDIEAIYQQPFNDLLYQAHTIHRTYHDPNSLQFATLLSIKTGACPEDCGY
CCCHHCCCHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCHHHEEEECCCCCCHHHCC
CSQSGHYKTHVEKEKLMSVEEVLQCAKEAKEGGAKRFCMGAAWRCPPDKAIPQLKEMIEG
CCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHCCCCEEHHCCCCCCCCHHHHHHHHHHHHH
VKSLGLETCMTLGMLTKEQASHLKEAGLDYYNHNIDTSPSYYDKVVTTRKFSDRLDTLNN
HHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
VRSAGINVCCGGILGLGETREDRIEFLLTLANMETPPESVPINRLIPVEGTPLAQAERVE
HHHCCCHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCEECCCCCCCHHHHHHH
GIELVRTIATARILMPKSAIRLTAGRTEMSDELQALCYFAGANSVFIGDKLLTEDNPQRF
HHHHHHHHHHHHHHCCCHHHEEECCCCHHHHHHHHHHHHCCCCEEEECCCHHCCCCHHHH
KDKTLFNKLGLTEMV
HHHHHHHHCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA