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The map label for this gene is dacB [C]

Identifier: 52841700

GI number: 52841700

Start: 1622738

End: 1624399

Strand: Direct

Name: dacB [C]

Synonym: lpg1470

Alternate gene names: 52841700

Gene position: 1622738-1624399 (Clockwise)

Preceding gene: 52841695

Following gene: 52841702

Centisome position: 47.76

GC content: 36.82

Gene sequence:

>1662_bases
ATGGAAATGCGAATGATGAAGAGATACTCGAAAAGATTACTGACTTATATTTATTTACTTATGAAAAGCATACCTCTAGT
GTTCACGATGCAACAACTATCATATAGCACAGATAAAATCCCTATACCAACCAATAAACTACCTGATGACCTAAACAAAG
TGATGAATAAATCTATTTATAAAAAAGCGTTGTGGGGATTACGTGTTGTTGATCTTGATACTGGTAATGTATTGCTGAAT
CTCAACTCAAGTACGCAGTTTTATATTGGTTCTGTGCGCAAGATATTTAGTGTAGGCGAACTATTGAATGAAAGAGGAGC
TGATTATCAATCTGTTACTAGAGTCCATTATGATGGTGTAGTACACAATGGTAATCTGAATGGGAATCTGGTACTCGTTG
CTTCAGGTGATTTGACCATGGGAGGACGAACTTTACCTGATGGACATGTCGCTATCACTGAATACGATCATAACGAGGCA
AATTCATTGGGTAATGCAATTATTACACAGACTAATCTTTTAGCAGGTTATCAATATCTCGCTAAACAAGTTTACAAAGC
TGGCATTCGCTCTATTTCAGGCGACATTGTGATAGACGATAGATTGTTTGAACCATTTAATTTCAGGGGTGAATTTGATG
TAACACCTATTTTCATAAACGATGATGTAGTTGATATTGTAATTCTTCCAAGTTTGATAAACGAGAATGCAAATGTATCG
TGGCTGCCACATTCATCGGCGTTCACCGTTATCAACCAACTTCTTACAGTTAATCCGCATGAAAAATATAATCTTGAACT
TGAGTCAAAATTACCTGCCTGCATAGGTAAACCAGGCTGTTCTGGCATCATTAAAGGCCATTTGCCTATCAATTTTATAC
CGCCACTTACTCACAACTACCCATTAGTACAAACTTTTCGAATCACTAAACCATCTAATTATGCCAGAACTATTTTTATC
GAGGCACTACAAGAAGCAGGCATCAACGTTTCATCGATTGATAAAACCAAAGAAAATCCGAAAAAACTACTAAAATCTAC
TAACTTGTACAATAAGAATAACAAAATTGCTGCTTTGAAATCATTGCCATTGGGAGAGCATGTAAAATATATTCTCAAAG
TTAGTTATAATATCGGAGCAGATACCAGCCTGGTGTTGTACGGACTAACAAAAGGCGCGCGCTCCATGATTGAAAGTCTA
AACGTGGAAAAAAAAATCCTGGATAAAAAATATGGCATATCCCCATCAGAATATCATTTTATTGATGGCAGCGGCGGCGG
CGAAACGAAGGCAACCAATACCGCTATAATAAAATGGCTTACTATAATGTCTAAAAGACCAGTATTTAACATATTTTACA
ATAGTCTGCCTATTCTTGCAGTAGATGGTTCACTTGGCTCAATTAATAACTTTCAATCAGATCCATCGCTTTCTGGGGCG
ACAGGTCGAGTGCATGCAAAAACTGGAACTTATGTATTGGGAAATGAGTCGGGTATGTTAGTTAAGGGGCGCTCTCTTGC
TGGTTACATTATTAGTAAAAAAAATCACCGACTTGCATTTGCCTTAGTTGTTAATAATATACCTATACAGTCACTAGAAG
ATTTATTTGCTGTAGCTCAGGATCAAGGCATAATAGCATCAATTCTATGGCGAGATTATTGA

Upstream 100 bases:

>100_bases
TTAATGTGATTTCTGCTAATTTCCCAATAATTTTTTTATCTGTGTAACAACAGTTATTATGCCAAATTTGACAAACCTAA
AAGAATAAAAACATATATCA

Downstream 100 bases:

>100_bases
TTATTAATATAAATAATATTTCATTCAACGATTGAAAGACATTATCGAAATGGAGATGTTTATAAAATAAACATTTGTCT
AATTTATTGTCCACTCAATA

Product: serine-type D-Ala-D-Ala carboxypeptidase

Products: NA

Alternate protein names: Peptidase; D-Alanyl-D-Alanine Carboxypeptidase; D-Ala-D-Ala Carboxypeptidase

Number of amino acids: Translated: 553; Mature: 553

Protein sequence:

>553_residues
MEMRMMKRYSKRLLTYIYLLMKSIPLVFTMQQLSYSTDKIPIPTNKLPDDLNKVMNKSIYKKALWGLRVVDLDTGNVLLN
LNSSTQFYIGSVRKIFSVGELLNERGADYQSVTRVHYDGVVHNGNLNGNLVLVASGDLTMGGRTLPDGHVAITEYDHNEA
NSLGNAIITQTNLLAGYQYLAKQVYKAGIRSISGDIVIDDRLFEPFNFRGEFDVTPIFINDDVVDIVILPSLINENANVS
WLPHSSAFTVINQLLTVNPHEKYNLELESKLPACIGKPGCSGIIKGHLPINFIPPLTHNYPLVQTFRITKPSNYARTIFI
EALQEAGINVSSIDKTKENPKKLLKSTNLYNKNNKIAALKSLPLGEHVKYILKVSYNIGADTSLVLYGLTKGARSMIESL
NVEKKILDKKYGISPSEYHFIDGSGGGETKATNTAIIKWLTIMSKRPVFNIFYNSLPILAVDGSLGSINNFQSDPSLSGA
TGRVHAKTGTYVLGNESGMLVKGRSLAGYIISKKNHRLAFALVVNNIPIQSLEDLFAVAQDQGIIASILWRDY

Sequences:

>Translated_553_residues
MEMRMMKRYSKRLLTYIYLLMKSIPLVFTMQQLSYSTDKIPIPTNKLPDDLNKVMNKSIYKKALWGLRVVDLDTGNVLLN
LNSSTQFYIGSVRKIFSVGELLNERGADYQSVTRVHYDGVVHNGNLNGNLVLVASGDLTMGGRTLPDGHVAITEYDHNEA
NSLGNAIITQTNLLAGYQYLAKQVYKAGIRSISGDIVIDDRLFEPFNFRGEFDVTPIFINDDVVDIVILPSLINENANVS
WLPHSSAFTVINQLLTVNPHEKYNLELESKLPACIGKPGCSGIIKGHLPINFIPPLTHNYPLVQTFRITKPSNYARTIFI
EALQEAGINVSSIDKTKENPKKLLKSTNLYNKNNKIAALKSLPLGEHVKYILKVSYNIGADTSLVLYGLTKGARSMIESL
NVEKKILDKKYGISPSEYHFIDGSGGGETKATNTAIIKWLTIMSKRPVFNIFYNSLPILAVDGSLGSINNFQSDPSLSGA
TGRVHAKTGTYVLGNESGMLVKGRSLAGYIISKKNHRLAFALVVNNIPIQSLEDLFAVAQDQGIIASILWRDY
>Mature_553_residues
MEMRMMKRYSKRLLTYIYLLMKSIPLVFTMQQLSYSTDKIPIPTNKLPDDLNKVMNKSIYKKALWGLRVVDLDTGNVLLN
LNSSTQFYIGSVRKIFSVGELLNERGADYQSVTRVHYDGVVHNGNLNGNLVLVASGDLTMGGRTLPDGHVAITEYDHNEA
NSLGNAIITQTNLLAGYQYLAKQVYKAGIRSISGDIVIDDRLFEPFNFRGEFDVTPIFINDDVVDIVILPSLINENANVS
WLPHSSAFTVINQLLTVNPHEKYNLELESKLPACIGKPGCSGIIKGHLPINFIPPLTHNYPLVQTFRITKPSNYARTIFI
EALQEAGINVSSIDKTKENPKKLLKSTNLYNKNNKIAALKSLPLGEHVKYILKVSYNIGADTSLVLYGLTKGARSMIESL
NVEKKILDKKYGISPSEYHFIDGSGGGETKATNTAIIKWLTIMSKRPVFNIFYNSLPILAVDGSLGSINNFQSDPSLSGA
TGRVHAKTGTYVLGNESGMLVKGRSLAGYIISKKNHRLAFALVVNNIPIQSLEDLFAVAQDQGIIASILWRDY

Specific function: Not Involved In Transpeptidation But Exclusively Catalyzes A Dd-Carboxypeptidase And Dd-Endopeptidase Reaction. [C]

COG id: COG2027

COG function: function code M; D-alanyl-D-alanine carboxypeptidase (penicillin-binding protein 4)

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 3.4.16.4; 3.4.99.- [C]

Molecular weight: Translated: 61280; Mature: 61280

Theoretical pI: Translated: 9.59; Mature: 9.59

Prosite motif: PS00146 BETA_LACTAMASE_A

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEMRMMKRYSKRLLTYIYLLMKSIPLVFTMQQLSYSTDKIPIPTNKLPDDLNKVMNKSIY
CCHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHCCCCCCCCCCCCCCCHHHHHHHHHHHH
KKALWGLRVVDLDTGNVLLNLNSSTQFYIGSVRKIFSVGELLNERGADYQSVTRVHYDGV
HHHHHCEEEEEECCCCEEEEECCCCEEEHHHHHHHHHHHHHHHHCCCCHHHHHEEEECCE
VHNGNLNGNLVLVASGDLTMGGRTLPDGHVAITEYDHNEANSLGNAIITQTNLLAGYQYL
EECCCCCCCEEEEECCCEEECCEECCCCCEEEEECCCCHHHHHCCCCEEHHHHHHHHHHH
AKQVYKAGIRSISGDIVIDDRLFEPFNFRGEFDVTPIFINDDVVDIVILPSLINENANVS
HHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCEEEEEECCCEEEEEEEHHHHCCCCCEE
WLPHSSAFTVINQLLTVNPHEKYNLELESKLPACIGKPGCSGIIKGHLPINFIPPLTHNY
EECCCHHHHHHHHHHCCCCCHHEEEEECCCCCHHHCCCCCCCEEECCCCEEEECCCCCCC
PLVQTFRITKPSNYARTIFIEALQEAGINVSSIDKTKENPKKLLKSTNLYNKNNKIAALK
CCEEEEEECCCCCCHHHHHHHHHHHCCCCHHHCCCCHHHHHHHHHHCCCCCCCCCEEEEE
SLPLGEHVKYILKVSYNIGADTSLVLYGLTKGARSMIESLNVEKKILDKKYGISPSEYHF
CCCCHHHEEEEEEEEECCCCCCEEEEEECCHHHHHHHHHCCHHHHHHHHHCCCCCCCEEE
IDGSGGGETKATNTAIIKWLTIMSKRPVFNIFYNSLPILAVDGSLGSINNFQSDPSLSGA
EECCCCCCCCCCHHHHHHHHHHHCCCCEEEEHHCCCCEEEECCCCCCCCCCCCCCCCCCC
TGRVHAKTGTYVLGNESGMLVKGRSLAGYIISKKNHRLAFALVVNNIPIQSLEDLFAVAQ
CCEEEECCCCEEEECCCCEEEECCCEEEEEEECCCCEEEEEEEECCCCHHHHHHHHHHHC
DQGIIASILWRDY
CCCCEEEHEECCC
>Mature Secondary Structure
MEMRMMKRYSKRLLTYIYLLMKSIPLVFTMQQLSYSTDKIPIPTNKLPDDLNKVMNKSIY
CCHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHCCCCCCCCCCCCCCCHHHHHHHHHHHH
KKALWGLRVVDLDTGNVLLNLNSSTQFYIGSVRKIFSVGELLNERGADYQSVTRVHYDGV
HHHHHCEEEEEECCCCEEEEECCCCEEEHHHHHHHHHHHHHHHHCCCCHHHHHEEEECCE
VHNGNLNGNLVLVASGDLTMGGRTLPDGHVAITEYDHNEANSLGNAIITQTNLLAGYQYL
EECCCCCCCEEEEECCCEEECCEECCCCCEEEEECCCCHHHHHCCCCEEHHHHHHHHHHH
AKQVYKAGIRSISGDIVIDDRLFEPFNFRGEFDVTPIFINDDVVDIVILPSLINENANVS
HHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCEEEEEECCCEEEEEEEHHHHCCCCCEE
WLPHSSAFTVINQLLTVNPHEKYNLELESKLPACIGKPGCSGIIKGHLPINFIPPLTHNY
EECCCHHHHHHHHHHCCCCCHHEEEEECCCCCHHHCCCCCCCEEECCCCEEEECCCCCCC
PLVQTFRITKPSNYARTIFIEALQEAGINVSSIDKTKENPKKLLKSTNLYNKNNKIAALK
CCEEEEEECCCCCCHHHHHHHHHHHCCCCHHHCCCCHHHHHHHHHHCCCCCCCCCEEEEE
SLPLGEHVKYILKVSYNIGADTSLVLYGLTKGARSMIESLNVEKKILDKKYGISPSEYHF
CCCCHHHEEEEEEEEECCCCCCEEEEEECCHHHHHHHHHCCHHHHHHHHHCCCCCCCEEE
IDGSGGGETKATNTAIIKWLTIMSKRPVFNIFYNSLPILAVDGSLGSINNFQSDPSLSGA
EECCCCCCCCCCHHHHHHHHHHHCCCCEEEEHHCCCCEEEECCCCCCCCCCCCCCCCCCC
TGRVHAKTGTYVLGNESGMLVKGRSLAGYIISKKNHRLAFALVVNNIPIQSLEDLFAVAQ
CCEEEECCCCEEEECCCCEEEECCCEEEEEEECCCCEEEEEEEECCCCHHHHHHHHHHHC
DQGIIASILWRDY
CCCCEEEHEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: Preferential cleavage is (Ac)2-L-Lys-D-Ala D-Ala and Also transpeptidation of peptidyl-alanyl moieties that are N-acyl substituents of D-alanine [C]

General reaction: Hydrolase; Acting on peptide bonds [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA